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Citations for Bio-Rad :
151 - 200 of 10000+ citations for GA binding protein alpha chain GABPA Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The polymerase chain reaction was carried out using primers (Supplementary Table S5) in a CFX96 amplifier (Bio-Rad, USA) using the following program ...
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bioRxiv - Bioengineering 2021Quote: We evaluated Blasticidin and RPP30 Copy Numbers using Droplet Digital™ Polymerase Chain Reaction (ddPCR) technology (Bio-Rad Technologies), according to manufacturer’s specifications ...
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bioRxiv - Cell Biology 2022Quote: ... Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) was performed using SsoAdvanced Universal SYBR® Green Supermix (1725274; BioRad) according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2022Quote: Quantitative polymerase chain reaction (qPCR) was conducted in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, USA), using 96-well PCR plates ...
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bioRxiv - Molecular Biology 2023Quote: ... for 2 min at temperatures from 36°C to 43°C with 1°C increments The samples were analyzed by 8% SDS-PAGE with Coomassie Blue staining and the collagen α1(I) and α2(I) chains were quantified using Image Lab 6.1.0 (Bio-Rad). Tm value was calculated with GraphPad Prism 9.3.1 (GraphPad Software ...
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bioRxiv - Immunology 2023Quote: ... Quantitative reverse-transcriptase polymerase chain reaction (qPCR) was performed on a CFX96 or CFX384 Real-Time System (Bio-Rad) using PerfeCTa SYBR Green SuperMix (Quanta Biosciences) ...
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bioRxiv - Molecular Biology 2024Quote: ... https://di-ning.com.cn/) was used for cDNA analysis on the iQ5 multicolor real-time Polymerase Chain Reaction Detection System (Bio-Rad). Data were analyzed using iQ5 2.0 standard optical system analysis software and the 2−ΔΔCT method (Livak and Schmittgen 2001).
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bioRxiv - Neuroscience 2020Quote: ... Aspecific binding sites were blocked for 30 min using TBS + 1% casein (BioRad, Temse, Belgium). Western blot was performed using the following primary antibodies overnight (4°C) ...
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bioRxiv - Cancer Biology 2020Quote: ... Unspecific binding was blocked by using PBS with 5% human AB serum (Bio-Rad, Germany) and 5% mouse serum (Agilent ...
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bioRxiv - Immunology 2020Quote: ... Binding of mAb to glycan was determined with A Bio-Plex 200 HTS (Bio-Rad) machine with the Bioplex manager software (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Specific binding was detected by enhanced chemoluminescence using Clarity Western ECL Substrate (BioRad, Hercules, CA) and visualized with a FUSION SOLO 4M system (Vilber Lourmat ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 μL of the binding mixture reactions were applied onto a Bio-Dot apparatus (BioRad) assembled with membranes pre-equilibrated with binding buffer without salts (top ...
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bioRxiv - Immunology 2020Quote: ... We used FITC-conjugated mouse anti-pig CD4 alpha (clone MIL17, Bio-Rad AbD Serotec, Puchheim, Germany, 1:20) and Alexa Fluor 647-conjugated mouse anti-pig CD8α (clone 76-2-11 ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins reacting with antibodies were detected with ECL Prime (Citiva) and visualized with ChemiDoc Touch (Bio-Rad). Acquired images were processed using Image Lab 5.2.1 (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The specific protein bands were visualized with a secondary antibody (anti-rabbit IgG-HRP conjugate; Bio-Rad) using a chemiluminescent substrate (Amersham ECL Western Blotting Detection Reagents ...
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bioRxiv - Cancer Biology 2024Quote: ... conjugated antibody was used to detect the desired protein by chemoluminescence with ECL (Bio-Rad, #170-5061).
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bioRxiv - Cell Biology 2024Quote: ... Proteins of interest were detected using HRP-conjugated secondary antibodies against mouse (1:7500, Bio-Rad Laboratories). The proteins were visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: Polymerase Chain Reaction (PCR) was carried out in 0.2 mL volume PCR in a thermal cycler (T100 Thermal cycler, Biorad, USA). HF Phusion Master mix (M0531S ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed using iTaq Universal SYBR Green qPCR Master Mix (Biorad, Hercules, CA), according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: A reverse transcription polymerase chain reaction (RT-PCR) was carried out on the RNA using the mix iScript Reverse Transcription Supermix (Biorad) and the following thermocycler program ...
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bioRxiv - Genetics 2021Quote: ... For quantitative real-time reverse-transcriptase polymerase chain reaction (qPCR) 20ng of cDNA per reaction and the iTaq Universal SYBR Green Supermix (Biorad) was employed ...
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bioRxiv - Neuroscience 2022Quote: ... was used for the Reverse Transcription-Polymerase Chain Reaction (RT-PCR) by CFX96 Touch qPCR Detection System (Bio-Rad, 1855196). Data was analysed by CFX Manager software (Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription-quantitative Polymerase Chain Reaction (RT-qPCR) was performed using TaqMan probes with iTaq Universal Probe Supermix on a C1000 Thermal Cycler (BioRad). The following TaqMan (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: The mRNA levels were assessed by real-time quantitative polymerase chain reaction (RT-qPCR) with Bio-Rad CFX96 (Bio-Rad), and a 20 μL of reaction ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed with the SYBR Green Supermix (Bio-Rad, Hercules, CA, USA) by a Bio-Rad CFX96™ Real-Time PCR System according to the manufacturer’s manual ...
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bioRxiv - Developmental Biology 2023Quote: ... we ran real-time quantitative polymerase chain reactions (qPCR) on a BioRad CFX96 Touch Real-Time PCR System (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2024Quote: ... Quantitative polymerase chain reaction (qPCR) analysis was performed with SsoAdvanced™ Universal SYBR® Green Supermix (#1725271; Bio-Rad Laboratories) and the following primers:
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bioRxiv - Biochemistry 2024Quote: DNA at selected regions of the genome were quantitated in isolated and desalted DNA fractions by quantitative polymerase chain reaction (qPCR) on the iCycler platform using the iTaq SYBR green supermix (BioRad). Primer pairs amplifying replication origins and corresponding adjacent background sites were synthesised by Sigma-Aldrich and used in PCR reactions at 0.5 µM for each primer ...
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bioRxiv - Cancer Biology 2024Quote: ... real-time quantitative polymerase chain reaction (RT-qPCR) was performed (CFX Connect and iTaq Universal SYBR Green SMX, Bio-Rad). All PCR reactions were performed with primers (Supplemental Table 1 ...
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bioRxiv - Neuroscience 2021Quote: ... Non-specific binding sites were blocked with 5% fat-free dry milk (Bio-Rad Hercules, California) in PBS containing 0.1% Tween-20 (PBST ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Single antigen-binding B cells were sorted using an S3 cell sorter (Bio-Rad, Hercules, CA), and deposited into a PCR tube with 5 μL lysis buffer (Takara Bio ...
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bioRxiv - Bioengineering 2024Quote: ... the membrane was incubated with HRP-conjugated anti-mouse secondary antibody (1:1000) and streptactin antibody (specific to the standard protein marker – Bio-Rad, Cat# 1610376; 1:1000 dilution) for 1 h at 25°C before washing it six more times ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was then washed thrice for 5 mins in PBS-Tween 20 before incubation with an HRP-conjugated anti-mouse secondary antibody (1:1000) and streptactin antibody (specific to the standard protein marker – Bio-Rad, Cat# 1610376; 1:1000 dilution) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Depletion of biotinylated proteins from lysates was confirmed by immunoblotting using Streptavidin HRP antibody (STAR 5B, Bio-rad).
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was performed using a CFX384 Touch Real-Time PCR Detection System (Bio-Rad). Quantification of mRNA expression was calculated by the DCT method with 18S ribosomal RNA as the reference gene.
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bioRxiv - Genetics 2020Quote: Polymerase chain reactions (PCR) were carried out on an MJ Research DNA Engine Tetrad PTC-225 (Bio-Rad Laboratories, Watford, UK). Forward and reverse primers were designed according to previously published work (Cothran et al. ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Real-time polymerase chain reaction was carried out in a final volume of 20 μl with iTaq Universal SYBR Green Supermix (Bio-Rad) containing 2 μl of five times diluted cDNA and monitored by CFX Connect Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) of the RNA was performed using a CFX96 Real-Time PCR Detection System (Bio-Rad) using the KAPA SYBR FAST One-Step qRT-PCR kit (Kapa Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... messenger RNA expression was analyzed by quantitative real-time polymerase chain reaction on a BioRad CFX machine using pre-validated primers with SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). GAPDH gene expression was used as the reference gene and the specificity of the assay was confirmed by melting curve analysis.
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bioRxiv - Immunology 2022Quote: ... Real-time polymerase chain reaction (PCR) was performed on a CFX96 real-time PCR detection system (Bio-Rad, Hercules, CA, USA) with SYBR Green PCR mix (Vazyme Biotech) ...
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bioRxiv - Immunology 2020Quote: ... cDNA was quantified using SYBR-green for quantitative reverse transcription polymerase chain reaction on a CFX Connect Real-Time System (Bio-Rad) with the following amplification scheme ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time quantitative reverse-transcription polymerase chain reaction (RT-qPCR) was performed using the iTaq Univer SYBR Green 1-Step Kit (Bio-Rad) on a StepOnePlus Real-time PCR system (Applied BioSystems ...
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bioRxiv - Cell Biology 2021Quote: ... Real-Time polymerase chain reaction (RT-PCR) was performed on the CFX 384 Touch™ Real-Time detection system (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... Amplification was performed through polymerase chain reaction (PCR) in a Bio-Rad C1000 TouchTM Thermal Cycler (BIO-RAD, Hercules, CA, USA). The V4 region of the 16S rDNA gene was amplified using dual-index (forward and reverse ...
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bioRxiv - Immunology 2024Quote: ... quantitative real-time polymerase chain reaction (qRT-PCR) using gene-specific primers and universal SYBR Green Supermix (#1725274, Bio-Rad Laboratories) was performed ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) assays were conducted on the CFX96 real-time detection system (Bio-Rad, USA) with the SYBR Green PCR Mix (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: The iTaq-Universal SYBR Green Supermix was used for quantitative reverse transcription-polymerase chain reaction (qRT-PCR) by following the manufacturer’s instructions (Bio-Rad, USA). The first strand cDNA samples were diluted 3 times prior to qRT-PCR ...
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bioRxiv - Physiology 2023Quote: ... was used to run the quantitative polymerase chain reaction (qPCR) using the CFX 380 Real-Time system C1000 TM Thermal Cycler (Bio-Rad), with the following parameters ...
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bioRxiv - Cell Biology 2023Quote: 5 to 10 ng of cDNA were used to perform quantitative polymerase chain reaction using 1X SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). All RT-qPCR reactions were performed in a CFX Connect Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Physiology 2024Quote: ... Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) was performed using a CFX96 Real-Time PCR Detection System (Bio-Rad) with Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) ...