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Citations for Bio-Rad :
1 - 50 of 10000+ citations for GA binding protein alpha chain GABPA Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Chain concentration was determined by DC protein assay (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... Beads were then transferred to fresh low- protein binding tubes and antibody-protein complexes were eluted by incubating in 60 μL 1x Laemmli Sample Buffer (Biorad, cat ...
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bioRxiv - Biochemistry 2022Quote: ... The binding to protein G magnetic beads (Biorad) was performed for 1 hour at 4 ºC (with previous washes with a RIPA buffer) ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... the light chain by the anti-human kappa light chain antibody (1:2,500; Biorad, cat.no. STAR 127) and GAPDH by the anti-GAPDH monoclonal antibody (1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibody binding was visualized using chemiluminescence (BioRad) on a BioRad ChemiDocTM Imaging System.
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bioRxiv - Cell Biology 2023Quote: ... we used primary alpha-tubulin rat antibody (Bio-rad) diluted 1:200 in 1% BSA in PBS for 1.5 hours at room temperature and secondary antibody anti-rat Alexa Fluor 647 (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... Protein binding was detected by using ECL substrate (BioRad) and visualised by ImageQuant4000 (GE Healthcare ...
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bioRxiv - Plant Biology 2022Quote: ... Goat anti-mouse Kappa Light Chain antibody (Bio-Rad, #105001G) and Goat Anti-Rabbit (IgG (H + L)-HRP Conjugate #1706515 ...
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bioRxiv - Plant Biology 2024Quote: ... Goat anti-mouse Kappa Light Chain antibody (Bio-Rad, #105001G) and Goat Anti-Rabbit (IgG (H + L)-HRP Conjugate #1706515 ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined by the coomasie dye-binding assay (BioRad) and enzyme activity was normalized to total protein.
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bioRxiv - Biochemistry 2021Quote: ... The protein concentration was determined using the Bradford protein dye binding assay (#5000113 + #5000114, Bio-Rad). 50μg of protein was separated by electrophoresis in an SDS-polyacrylamide gel which was then transferred using the semi dry method ...
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bioRxiv - Neuroscience 2022Quote: ... Antibody binding was detected by chemiluminescence(Clarity kit, Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... Antibody binding was detected by chemiluminescence (Clarity kit, Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... Proteins were quantified with the Coomassie dye binding method (BioRad, 500-0006) as described by the manufacturer.
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bioRxiv - Cell Biology 2020Quote: ... the process was repeated with an alpha-tubulin antibody (BioRad MCA78G, 1:2000) to minimize cross-reaction ...
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bioRxiv - Cell Biology 2020Quote: ... The primary antibody binding was processed for ECL detection (Bio-Rad) with appropriate HRP-conjugated secondary antibodies (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2021Quote: ... Antibody binding was visualised using Clarity Western ECL Substrate (Biorad, UK) and viewed under the Gel Doc imaging system (GeneSys software ...
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bioRxiv - Microbiology 2024Quote: ... Antibody binding was visualised using Clarity Western ECL Substrate (Biorad, UK) and viewed under the Gel Doc imaging system (GeneSys software ...
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bioRxiv - Biochemistry 2020Quote: ... Protein concentrations were determined by the dye-binding method of Bradford assay (Bio-Rad) and bovine serum albumin as the calibration standard ...
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bioRxiv - Cell Biology 2024Quote: ... The pull-down binding proteins on beads were mixed with loading buffer (Bio-Rad). The beads mixture was loaded into 4-20% gradient gel for SDS-PAGE analysis ...
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bioRxiv - Systems Biology 2020Quote: ... Unspecific antibody binding was blocked with PBS/10% AB-serum (Bio-Rad) for 5 min at 4°C prior to staining with CD19 or CD3 ...
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bioRxiv - Cell Biology 2023Quote: ... Antibody binding was detected using an ECL chemiluminescence kit (Bio-Rad, 1705061). The following antibodies were used ...
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bioRxiv - Neuroscience 2024Quote: ... Antibody binding was detected using an ECL chemiluminescence kit (Bio-Rad, 1705061). In order to detect multiple proteins for the same set of samples ...
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bioRxiv - Microbiology 2024Quote: ... The antibody binding was detected using Clarity™ Western ECL substrate (Bio-Rad). Western blot experiments ...
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bioRxiv - Immunology 2020Quote: ... 18.10 is a murine IgG1 anti-human HVEM mAb and was produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-rad). The mouse IgG1 isotype control (clone MOPC-21 clone) ...
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bioRxiv - Immunology 2023Quote: ... and membrane GAS protein fractions were separated on SDS-PAGE (Criterion TGX Gels, 4%–20% precast gels, Bio-Rad) and proteins were transferred to PVDF membranes using the trans-blot turbo transfer system (BioRad ...
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bioRxiv - Microbiology 2021Quote: ... The antibody binding was imaged with Clarity Western ECL Substrate (Bio-Rad, CA, USA).
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bioRxiv - Cell Biology 2024Quote: ... The antibody binding was assessed with a ChemiDoc Imaging System (Bio-Rad, Madrid, Spain) and the density of bands was measured using ImageJ software v.1.8.0_172 (NIH ...
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bioRxiv - Microbiology 2022Quote: ... anti-alpha-Tubulin (VMA00051, BIO-RAD), anti-Lamin B1 (66095-1-Ig ...
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bioRxiv - Cell Biology 2020Quote: ... alpha-Tubulin (BioRad MCA78G, 1:2000), Cdc20 (Santa Cruz sc-8358 ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using a colorimetric assay based on the Bradford dye-binding method (Bio-Rad Protein Assay Dye Reagent ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were incubated with goat anti-rabbit IgG (H+L chains) HRP-conjugated secondary antibodies (Bio-Rad) and detected using SuperSignal West Pico ECL (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-Rad, Marnes-La-Coquette, France). Mouse IgG1 isotype control was purchased from Miltenyi Biotec (Bergisch Gladbach ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHHs was detected using a mouse anti-HIS-tag antibody (Biorad, MCA1396, 1/2000) and an AF647 conjugated donkey anti mouse IgG antibody (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... antibody binding was detected with either the Clarity or Clarity Max Western ECL substrate (Bio-Rad) and ChemiDoc MP Imaging System (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were blocked for 1hr in PBS with 1mg/ml and 0.1% triton and then stained overnight with anti-alpha Tubulin antibody (1:100, MCA78G, BioRad). Cells were then washed ...
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bioRxiv - Plant Biology 2023Quote: ... Ten µl of the supernatant were used to determine protein concentrations by the dye-binding Bradford assay (Bio-Rad). Twenty-five µg of total proteins in a final volume of 100 µl were pipetted into a white 96 well plate (Greiner Bio-One ...
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bioRxiv - Microbiology 2021Quote: ... Binding of the anti-NTD antibody was detected using a goat anti-human kappa light chain conjugated to horseradish peroxidase (HPR) (BioRad). To normalize for the amount of the bound probes ...
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bioRxiv - Cell Biology 2022Quote: ... a DNA-binding fluorescent dye (BioRad, SsoAdvanced Universal SYBR Green Supermix ...
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bioRxiv - Cell Biology 2022Quote: ... Total cell lysates were prepared with icecold lysis buffer and the protein concentration was measured by Coomassie binding (Bio-Rad). Equal amounts of protein were added to each well of a G-LISA plate and incubated for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: All binding assays were performed on a ProteOn XPR36 SPR Protein Interaction Array System (Bio-Rad Laboratories, Hercules, CA, USA). The instrument temperature was set at 25 °C for all kinetic analyses ...
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bioRxiv - Immunology 2020Quote: ... The binding was detected by an HRP-conjugated anti-human IgG (Fc) CH2 Domain antibody (Bio-Rad MCA647P) used at a 1:5,000 dilution at RT for 1 h and developed as described above.
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bioRxiv - Biochemistry 2024Quote: ... Antibody binding was visualized using Clarity Western ECL Substrate and a ChemiDoc Touch Imaging System (both Bio-Rad).
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bioRxiv - Microbiology 2020Quote: Surface Plasmon Resonance (SPR) binding experiments were carried out on a ProteOn XPR36 6×6 channels protein interaction array system (Bio-Rad). Proteins BRD4-BD1 an BRD4-BD2 were diluted to a final concentration of 50μg/ml in HEPES buffer 25 mM pH 7.0 and immobilized to a GLM chip using amine coupling ...
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bioRxiv - Immunology 2020Quote: ... and IgA (heavy chain specific; Bio-Rad, Hercules, CA, USA) secondary antibodies diluted 1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... using a DNA-binding fluorescent dye (BioRad, SsoAdvanced Universal SYBR Green Supermix ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and stored at -80 until α5 subunit and its binding partners were immunoprecipitated using SureBeads protein G magnetic beads according to the manufacturer’s protocol (Biorad, Hercules, CA, USA). Western blots (detailed in section 2.8 ...
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bioRxiv - Biophysics 2024Quote: ... The pellet was then resuspended in binding buffer (50mM Tris, 120 mM NaCl, pH 7.4) Bradford protein assay (Bio-Rad, Hercules, CA) was used to determine the protein concentration and the membranes were diluted to 1000 μg/mL and stored in a −80 °C freezer for later use ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using a colorimetric assay based on the Bradford dye-binding method (Bio-Rad Protein Assay Dye Reagent, Bio-Rad Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the whole cell membrane pellets were resuspended in buffer and protein concentration was estimated by dye binding (Coomassie Brilliant Blue R-250; Bio-Rad, USA). The binding reaction was carried out in a final volume of 0.1 ml of Na+-containing buffer (20 mM Tris-HCl ...