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Citations for Bio-Rad :
1901 - 1950 of 6365 citations for Mouse Cytochrome C Oxidase Subunit II COX2 MT CO2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Samples were electrophoretically resolved on gelatin-containing gels at 125 V for 110 min at 4 °C using a Power Pack supply (Bio-Rad, USA) Next ...
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bioRxiv - Microbiology 2020Quote: ... The suspension was mixed on an orbital shaker for 60 – 90 minutes at 4 °C and then transferred to a glass column (BIO-RAD EconoColumn). Non-His-Tagged proteins were eluted with lysis buffer until the absorbance at 280 nm matched background levels utilizing 50 – 100 mL of lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... the plates were incubated at 37°C for 24 h in ambient air and growth was assessed at OD595 using microplate reader (iMark, Bio-Rad, Japan). The lowest concentration that completely prevented bacterial growth was categorized as MIC.
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were reduced with 1 M NaBH4 in 50 mM KOH for 3 h at 50°C then desalted and enriched offline using AG 50W-X8 (Bio-Rad, Australia) strong cation exchange followed by porous graphitized carbon (PGC ...
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bioRxiv - Molecular Biology 2022Quote: ... The synthetic ssDNA oligo was converted to dsDNA by primer extension reaction at 60° C using iProof DNA polymerase (Bio-Rad, USA). The resulting duplex was cloned between the EcoRI and HindIII sites of the pUC57 vector ...
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bioRxiv - Developmental Biology 2022Quote: ... lieberkuehni developmental stages were mixed in 2x Laemmli sample buffer with 10 % 2-β-mercaptoethanol and heated at 97 °C for 5 min before loading on a 12 % (w/v) TGX FastCast SDS/PAGE gel (Bio-Rad, USA). Membranes were blocked in 3 % (w/v ...
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bioRxiv - Neuroscience 2019Quote: ... and samples were then transferred at 100 V at 4°C onto an activated 2 um polyvinylidene difluoride (PVDF) membrane (Bio-Rad 1620177), blocked with 3% BSA in 0.2% Tween-20 (Sigma P9614 ...
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bioRxiv - Microbiology 2019Quote: ... followed by 40 cycles at 95°C for 10 seconds and 60°C for 30 seconds in a Bio-Rad CFX96™ Real-Time System coupled to a C1000™ Thermal Cycler (BioRad). Expression of target genes was normalized by the β-actin endogenous transcript levels for each RNA prevenient from the culture conditions [47] ...
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bioRxiv - Developmental Biology 2019Quote: Oocytes were collected in 0.1% PVP in DPBS and boiled for 3 mins at 95°C in 1x Laemmli Sample Buffer (Bio-Rad, 161-0747) supplemented with β-mercaptoethanol ...
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bioRxiv - Microbiology 2019Quote: ... pH 8 for 1 h at 37°C and then loaded into the wells of 1% pulse-field certified agarose gel (Bio-Rad Laboratories) in 0.5X TBE (45mM Tris HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein samples were combined with the 4x Laemli sample buffer (900 μl of sample buffer and 100 μl β-Mercaptoethanol), heated (95°C, 5mins) and run on SDS-PAGE (protean TGX pre-casted gradient gel, 4%-20%, Bio-rad) and transferred to the Nitro-Cellulose membrane (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Final extension was at 72°C for 7min and the reaction was stopped by holding tubes at 4°C until removed from the thermal cycler (MyCycler™ Thermal Cycler BIORAD, USA). The PCR products were electrophoresed in a 1.5% agarose gel stained with SYBR Safe DNA Gel stain (Invitrogen ...
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bioRxiv - Bioengineering 2020Quote: ... the 96-well microplate was incubated at 37 °C for 1h and the end-point image was taken by ChemiDoc™ MP Imaging System (Bio-Rad).
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bioRxiv - Bioengineering 2020Quote: ... The DAMR systtem for one-pot RPA/CRISPR-Cas12a detection was incubated in PCR tubes at 37 °C and the real-time fluorescence signal was monitored by CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad). After reaction ...
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bioRxiv - Molecular Biology 2019Quote: ... Aliquots of 50 μg protein in SDS-PAGE sample buffer containing 0.2 M dithiothreitol were heat-denatured for 5 min at 95 °C then electrophoresed on AnyKD midi criterion™ gels (Bio-Rad) in ProSieve™ EX running buffer (Lonza) ...
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bioRxiv - Biochemistry 2021Quote: ... The membranes were incubated in the dark with the CSPD reagent for 30 min at 37 °C before imaging with ChemiDoc™ MP Imaging System (BIO-RAD). tRNA-Glu northern blot was synthesized by IDT and the sequence was listed below ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were centrifuged at 20,000 x g for 10 min at 4°C and protein concentration in the supernatant was determined by Bradford assay (Bio-Rad, 5000202) and normalized across samples ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were heated to 95°C for 5 min and then analyzed on a 4-12% gel (Bio-Rad Laboratories, Hercules, CA) using SDS running buffer ...
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bioRxiv - Immunology 2019Quote: ... Upfront tagmentation of ~35,000 nuclei was performed for 1 h at 37°C in a C1000 Touch thermal cycler with 96-Deep Well Reaction Module (Bio-Rad, 1851197). Tagmented nuclei were mixed the master mix and loaded onto a Chromium Chip with Gel beads and partitioning oil on different wells ...
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bioRxiv - Cell Biology 2019Quote: ... The reaction was quenched by incubation with 1 mM TCEP for 20 minutes at 37°C and 60% of the eluate was run on an SDS-PAGE gel (Mini-Protean TGX, 4-15%, Bio-Rad #4561084). The gel was stained with colloidal Coomassie (Bio-Rad #1610803 ...
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bioRxiv - Bioengineering 2022Quote: ... pH 8.3 at 100 V for 2 h at 2-8 °C using PowerPac Basic setup (Bio-Rad Laboratories Inc., Hercules, CA). Post-electrophoresis ...
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bioRxiv - Cancer Biology 2022Quote: ... 5’ - GAT GGA GAA AGC TCT GAG CAT C -3’ Reverse: 5’ - TTG CTC CAC AGA TGG AGT TG -3’ RHAMM PrimePCR primers were obtained from BioRad (Cat#: 10025636)
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bioRxiv - Immunology 2022Quote: ... aliquots of frozen mEVs were thawed at 37°C and Bio-Rad Protein Assay (Bradford 1976) was performed following manufacturer’s instructions (500-0006 Bio-Rad. München, Germany). Samples were diluted with filtered PBS 1:50 and bovine serum albumin (BioRad Laboratories ...
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bioRxiv - Genetics 2022Quote: ... Signals were visualized using X-ray film and a film processor (pSer63 c-Jun) or using Chemidoc™ MP Imaging System (Bio-Rad). Membranes were re-incubated with anti α-Tubulin (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Table 1) was performed at 4°C overnight at a 1:1000 dilution in 5% nonfat dry milk (Bio-Rad No. 1706404). Incubation of HRP conjugated secondary antibody (different species ...
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bioRxiv - Neuroscience 2022Quote: ... while brain cytokine analyses for Studies C and D were conducted using a custom 10-plex Bioplex (Bio-rad Laboratories, Hercules, CA) as previously described (Finnell ...
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bioRxiv - Cell Biology 2022Quote: The GST-FERRY interactor screen was performed at 4 °C in gravity flow filter columns (Poly- Prep® Chromatography Column, Bio-Rad). 500 μl Glutathione Sepharose 4B (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were eluted from sepharose beads by boiling at 95 - 100 °C for 5 min with 40 µl sample buffer diluted from 4x sample buffer (Bio-Rad, 1610747) with Lysis Buffer ...
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bioRxiv - Plant Biology 2023Quote: ... The samples were then separated by 6.5% (w/v) native polyacrylamide gels at 4 °C and the biotin-labeled probes was captured with ChemiDoc™XRS+ (Bio-Rad). The oligonucleotide probes used in this study are listed in Supplemental Data Set S1.
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bioRxiv - Biochemistry 2023Quote: ... applying a constant current of 300 mA at 4°C for 1 hour using a Mini Trans-Blot® Cell (Bio-Rad). For the immunodetection of specific protein targets ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein samples were boiled for 10 minutes at 95 °C and loaded onto Mini-PROTEAN TGX™ Precast Gels (BIO-RAD:4569036), and electrophoresis was performed according to manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2023Quote: ... The denatured lysate was then clarified by centrifugation (13200 rpm, 10 min at 4°C) and protein concentration was determined using the DC™ Protein Assay (Bio-Rad). Either equal amounts or a serial dilution of the lysates from various strains were resolved using SDS-PAGE and transferred onto a polyvinylidene difluoride membrane ...
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bioRxiv - Biochemistry 2024Quote: ... were annealed at a ratio of 3:1 molar ratio Incubated at 93°C for 3 minutes and slowly cooling down in C1000 Touch™ Thermal Cycler (Bio-Rad). The pre-annealed RNA-TS (32 µM ...
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bioRxiv - Biochemistry 2023Quote: ... followed by heat-inactivation at 75°C for 5 min and desalting using Micro Bio-Spin P-6 columns (Bio-Rad, 7326221). Dephosphorylated guide RNAs were then radiolabeled using T4 Polynucleotide Kinase (0.33 U/µL ...
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DNA Recognition and Induced Genome Modification by a Hydroxymethyl-γ Tail-Clamp Peptide Nucleic AcidbioRxiv - Genetics 2023Quote: ... Droplets were allowed to rest at 4 °C for at least 30 min after cycling and were then read using the QX200 ™ Droplet Reader (Bio-Rad). Data were analyzed using QuantaSoft™ software ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were stained in different cycles overnight at 4°C with the following antibodies: anti-CD3 (clone CD3-12; #MCA1477, Bio-Rad, USA), anti-CD206 (clone E6T5J ...
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bioRxiv - Molecular Biology 2023Quote: ... the sample boiled for five minutes at 95 °C and size-separated by SDS-PAGE using a 4–20% Mini-PROTEAN® TGX™ Gel (Biorad). Proteins were transferred onto a nitrocellulose membrane (Biorad ...
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bioRxiv - Biochemistry 2022Quote: ... adjusted to equal concentration for each sample and boiled at 95°C for 10 minutes in Laemmli buffer (Bio-Rad #161-0747).
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bioRxiv - Bioengineering 2023Quote: ... before injecting into 1 mL Hitrap Heparin HP column pre-equilibrated with Wash Buffer C operated in the BioLogic DuoFlow™ FPLC system (Bio-rad). The protein was eluted from the column by running a gradient flow rate that exchanges Wash Buffer C and Elution Buffer D (2000 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were heated to 50°C for 2 to 3 min before the addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594), vortexed vigorously for 5 sec before pipetting in plug mould (Bio-Rad 1703713) ...
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bioRxiv - Biophysics 2023Quote: ... The mixture was incubated for 1-2 h at 4 °C and incubated overnight with pre-washed bio-beads (Bio-Rad Laboratories) at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... alum pellets were resuspended in sample loading buffer and boiled at 95 °C for ten minutes before being applied to a precast polyacrylamide gel (4–20% Mini-PROTEAN®, Bio-Rad). Proteins were then transferred to a nitrocellulose membrane using the Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... heated up for 10 minutes at 95°C and centrifuged before loading onto a 10 well 5-20% gradient gel (BioRad 456-1093). The finished gels were blotted via semi dry method (20V ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were washed 3 times with ice-cold PBS and boiled at 90 °C in 2x Laemnli sample buffer (Bio-Rad 1610737) for 5 minutes the following day for elution.
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at full speed for 10 minutes at 4⁰C and the total protein concentration in the supernatant was quantified using the detergent compatible (DC) protein assay (BioRad, 500-0114). Equal amounts of protein were mixed with anionic detergent (4x LDS loading buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were boiled at 95°C for 5 min and loaded into 4-12% Criterion XT-Bis-Tris polyacrylamide gels (Bio-Rad 3450125). Gel electrophoresis was performed in 1X MES running buffer (Bio-Rad 1610789 ...
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bioRxiv - Neuroscience 2023Quote: ... The cell suspension was collected and centrifuged at 4°C for 15 minutes at 10,000g and cell protein extracts were stored at −80°C or brought forward for total protein quantification using Bradford assay (Bio-Rad; USA). Cell protein samples were suspended in 2x Laemmli buffer (Bio-Rad ...
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bioRxiv - Genetics 2023Quote: ... and 0.2 μl of 10X NEB 2.1 buffer were mixed gently in a PCR tube and incubated at 25°C for 15 minutes in a Thermal Cycler (Bio-Rad T100) and then 1 μL ssDNA repair template [IDT] and Phenol red (0.3 μL ...
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bioRxiv - Biophysics 2024Quote: ... This mixture was then incubated at 4 °C for 1 hour and further subjected to a 4-hour incubation with bio-beads (Bio-Rad, USA). After incubation ...
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bioRxiv - Biochemistry 2024Quote: ... and boiled at 95° C for 5 minutes before separation on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad). In-gel fluorescence was imaged first ...