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Citations for Bio-Rad :
2101 - 2150 of 6365 citations for Mouse Cytochrome C Oxidase Subunit II COX2 MT CO2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... were centrifuged at 5000 x g for 15 min at 4°C and resolved by SDS-PAGE prior to transfer to a PVDF membrane (BioRad, Hercules, CA, USA). The membrane was then incubated with SnTox3 primary antibodies ...
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bioRxiv - Physiology 2021Quote: ... The solution with MSP1E3D1 was mixed at 4°C for 10 min before addition of 130 mg of Biobeads SM2 (Bio-Rad, Hercules, CA). Biobeads (washed into methanol ...
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bioRxiv - Plant Biology 2021Quote: ... from Eurofins Genomics (https://www.eurofinsgenomics.eu/) were mixed and melted at 95◻°C for 5◻min in TE buffer in a T100™ thermocycler (Bio-rad). Then the denatured oligonucleotides were allowed to anneal as the temperature was reduced by 1 °C every 20 sec ...
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bioRxiv - Biochemistry 2020Quote: Surface biotinylation samples were heated to 50°C for 10 minutes before separation using 4-20% polyacrylamide gradient gels (Bio-Rad, Hercules, CA). After separation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were heated at 95°C for 5 min prior to loading onto 4-20% Mini-PROTEAN® TGX™precast polyacrylamide gel (Bio-Rad). Gel electrophoresis was done at 100 V for 90 min ...
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bioRxiv - Neuroscience 2019Quote: ... RNA probes were then alkaline hydrolyzed (80mM NaHCO3, 120mM Na2CO3) for 60°C for 15 minutes and purified using a microcolumn (Bio-Rad 732-6223). Probe integrity was assessed by agarose gel and kept at −80°C when not in use.
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bioRxiv - Bioengineering 2021Quote: ... samples of 5-10 μg protein were reduced with 50 mM dithiothreitol (DTT) for 15 minutes at 90°C and mixed with 4× sample loading buffer (Bio-Rad, Munich, Germany). Protein separation was carried out via SDS-PAGE in 12% polyacrylamide gels (Mini-PROTEAN® TGX™ Precast Gels ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated briefly at 90°C and then transferred to a Trans-Blot Turbo mini nitrocellulose membrane using Trans-Blot Turbo Transfer system (Bio-Rad, Hercules, CA). The membrane was incubated overnight in antibody buffer (50mM tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: Cellular lysates were incubated at 95°C for 5 min and separated in 12% Mini-PROTEAN TGX Precast Protein gels (BIO-RAD, Cat #4561044). Proteins were then transferred to 0.45 μm PVDF membrane at 100V for 1 hr ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the 30°C incubation step was shortened to 5 hr using a Bio-Rad C1000 Touch thermal cycler (Bio-Rad, Hercules, CA, USA). DNA concentrations were measured by Qubit ...
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bioRxiv - Molecular Biology 2020Quote: ... Sample DNA was digested at 37°C for 15 minutes and loaded in ddPCR DG8™ Cartridge with QX200™ droplet generation oil (Bio-Rad). Droplets were made using QX200™ droplet generator (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... the 30°C incubation step was shortened to 5 hr using a Bio-Rad C1000 Touch thermal cycler (Bio-Rad, Hercules, CA, USA). DNA concentrations were measured by Qubit ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were heated 5 min at 95°C and separated on 4-20% Mini-PROTEAN® TGX Stain-Free™ Precast gels (Bio-Rad) or on home-made 7% polyacrylamide gel for OPA1 immunodetection ...
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bioRxiv - Molecular Biology 2020Quote: ... and the sample incubated at 90°C for 5 min and resolved by electrophoresis through a 4–20% gradient polyacrylamide/SDS gel (Bio-Rad Laboratories, 5671085). Next ...
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bioRxiv - Biochemistry 2022Quote: REST-FL was purified in two-step chromatography and carried out in a cold room at 4°C with a chromatography system (NGC™ Chromatography System Quest™ 10 Plus, Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... carried out in a cold room at 4°C with a chromatography system (NGC™ Chromatography System Quest™ 10 Plus, Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... Expression of tGFP was monitored for 5 h at 30°C using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Inc.).
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bioRxiv - Molecular Biology 2022Quote: ... followed by a final extension step at 68 °C for 5 min on the T100™ thermal cycler (Bio-Rad, Hercules, CA, USA). During protocol optimization ...
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bioRxiv - Biochemistry 2024Quote: ... The fluorescence signal was measured at 30 s intervals at 37°C using a CFX96 touch real-time PCR system (Bio-Rad, CA, USA).
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bioRxiv - Biochemistry 2023Quote: ... and incubated at 4 °C for 30 min and heated at 37 °C for 5 min prior to analysis on precast “any KD” Mini-PROTEAN TGX (Bio-rad, Cat #4569033) polyacrylamide gels using 1x TBE as running buffer ...
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bioRxiv - Bioengineering 2023Quote: ... and boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Samples were heated for 10 min at 70°C and then loaded on a 12% Mini-protean TGX gel (Bio-Rad Cat# 4561044). The gels were run for approximately 1 h at 100 V ...
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bioRxiv - Cell Biology 2023Quote: ... then centrifuged at 13,000 rpm for 30 min at 4 °C and the resulting supernatants were subjected to Bradford protein assay (Bio-Rad, Hercules, California, USA) for measuring total protein concentration ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were denatured at 95 °C for 3 minutes prior to loading onto a 16.5% Mini-PROTEAN® Tris-Tricine Gel (Biorad Cat. No. 4563066). Gels were transferred to a nitrocellulose membrane (Millipore Sigma Cat ...
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bioRxiv - Physiology 2023Quote: ... 50µL from this mixture was transferred to a new tube containing 950µL of PBS and stored at 5°C for subsequent the cell counting (using a TC20 Automated Cell Counter, Bio-Rad, Solna, Sweden) 10 to 30 min later (when the mitochondrial measurements had started) ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative real-time PCR (RT-qPCR) analysis was performed using a CFX384 Real-Time System (C 1000 Touch Thermal Cycler, Bio-Rad, Hercules, California) using SensiFAST SYBR No-ROS kit (Bioline ...
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bioRxiv - Neuroscience 2023Quote: ... heated for 5 min at 95°C and loaded onto 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Germany). Proteins were transferred on nitrocellulose membranes and membranes were blocked with 5% BSA in PBS-Tween ...
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bioRxiv - Microbiology 2023Quote: ... heated in water bath for 5 minutes at 99°C and analysed by SDS-PAGE using Any-kD Mini-PROTEAN TGX Stain-Free gels (Bio-Rad, California, USA) in a 2-minute run for sample clean-up purposes ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants were collected and stored at −80□°C and cells were lysed in 1x Laemmli Buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA) for further analysis.
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at 16,000Xg for 30 min at 4°C then protein concentrations were determined by Quick Start™ Bradford 1x Dye Reagent (Bio-Rad #5000205). For each sample ...
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bioRxiv - Genetics 2023Quote: ... Co-IP reactions were rotated overnight at 4°C with 100 µl of SureBeads Protein G Magnetic Beads (BioRad; Catalog# 1614013; Hercules, CA). The bead lysates were washed five times in the co-IP buffer using a DynaMag Magnet (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: ... at 100V with a constant current for 60 mins at 4°C in a Bio-Rad Mini-Transfer Cell (Bio-Rad, Cat# 1703930) according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C in a humidified chamber with the following primary antibodies: Guinea-pig anti-Insulin antibody (Biorad 5330-0104G, 1:250). Following overnight incubation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We incubated the reaction for 1 hour at 50°C in a thermal cycler (Bio-Rad C1000 Touch Thermal Cycler, Bio-Rad, USA).
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bioRxiv - Cell Biology 2023Quote: ... and the lysates were heated for 15 minutes at 50 °C before being loaded onto 4-15% or 4-20% Mini-PROTEAN SDS-PAGE gels (Bio-Rad, Hercules, CA) in 1X TGS solution (250 mM Tris ...
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bioRxiv - Developmental Biology 2024Quote: ... and the reactions were stopped by boiling them at 95°C for 5 minutes after adding Laemmli sample buffer (Bio-Rad, 161-0747) supplemented with β-mercaptoethanol (Gibco 21985023) ...
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bioRxiv - Cell Biology 2023Quote: ... denaturated at 95 °C for 5 min and separated on Bis-Tris polyacrylamide gels using the Mini-PROTEAN® Tetra Handcast System (Biorad, cat.no. 1658029FC). For visualization of total protein load ...
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bioRxiv - Physiology 2023Quote: ... heated (70°C for 5 min) and loaded (10 µg per lane) onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA). Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min ...
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Post-stroke rapamycin treatment improves post-recanalization cerebral blood flow and outcome in ratsbioRxiv - Neuroscience 2023Quote: ... 50 µg of protein was denatured (95°C for 5 minutes) using lysis and Laemmli sample buffer (161-0737, Bio-Rad, with DTT). Samples were separated on a 10 % gradient gel (Criterion TGX Precast Gel ...
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bioRxiv - Neuroscience 2023Quote: ... and boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... samples were placed on a heat block (+90°C) for 10 minutes and separated by SDS–polyacrylamide gel electrophoresis (4–20% Mini-PROTEAN TGX gels, Bio-Rad, 456-1096). Proteins were transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... was then added and the samples were boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Samples were boiled at 95°C for 5 min and resolved in 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). Proteins were transferred to a nitrocellulose membrane and probed overnight at 4°C with primary antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... blots were incubated with 1: 5000 dilution of HRP-conjugated anti-mouse/ rabbit IgG secondary antibodies (Bio-Rad) in 1% milk-TBST for another hour ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were rinsed and incubated with the fluorescent secondary antibodies StarBright Blue 700 anti-mouse (1:5.000, BioRad) and StarBright Blue 700 anti-rabbit (1:5.000 ...
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bioRxiv - Genomics 2020Quote: ... Secondary antibody used were an HRP-conjugated goat anti-mouse or rabbit at 1:25,000 (BioRad, Hercules, CA).
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bioRxiv - Genetics 2019Quote: ... followed by an anti-mouse (Abliance; 1/1,000) secondary antibody and then the Clarity Western ECL reagent (BioRad). Densitometric analysis was performed on non-saturated signals using the Image Lab™ software (BioRad).
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were washed extensively with PBS-T before incubated with goat anti-mouse-HRP (Bio-Rad Laboratories) or rabbit anti-sheep-HRP (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cells were labeled with a mouse monoclonal anti-Chlamydia LPS antibody (BioRad CF 6J12, Hercules, CA, USA), rinsed with 1x PBS ...
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bioRxiv - Microbiology 2020Quote: ... Metabolically active hormones were quantified using the Bio-Plex Pro Mouse Diabetes Panel (Bio-Rad Laboratories, Hercules, CA). Both were prepared according to the manufacturer’s instructions ...