Labshake search
Citations for Bio-Rad :
1801 - 1850 of 1976 citations for MBL 2 Mouse HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal anti-MF20 (DSHB, Cat# MF 20, dilution 1:20) rabbit polyclonal anti-Calcitonin Receptor (Bio-RAD, Cat# AHP635, dilution 1:2000), chick anti-GFP (abcam ...
-
bioRxiv - Immunology 2023Quote: Extracted whole-kidney protein was diluted in PBS to 900 µg/mL and subjected to a Bio-Plex Mouse Cytokine 23-plex Assay (#M60009RDPD, Bio-Rad, Hercules, CA), according to the manufacturer instructions ...
-
bioRxiv - Physiology 2023Quote: ... Cytokine concentrations in BAL fluid were measured using the multiplex Bio-Plex 200 System and Bio-Plex Pro Mouse Cytokine/Chemokine Immunoassays (Bio-Rad, Hercules, CA).
-
bioRxiv - Molecular Biology 2023Quote: ... primary antibodies and proteins were detected by incubation at a 1:5000 dilution with secondary horseradish peroxidase (HRP)- conjugated antibodies (#170-6516, goat anti-mouse and 170-6515; goat anti-rabbit from Bio-Rad, Hercules, USA). The reactive protein bands were visualized using the WesternBright ECL HRP substrate (Advansta ...
-
bioRxiv - Molecular Biology 2023Quote: ... and optic density of the bands corresponding to mouse PRM1 and PRM2 was quantified using Quantity One 1-D analysis software (BioRad, Hercules, CA, USA) to calculate the PRM1/PRM2 ratio ...
-
bioRxiv - Immunology 2023Quote: ... Primary antibody staining was performed overnight at 4&C using rabbit anti-mouse Iba-1 (1:500; Wako Chemical Cat #019-19741) and CD68 (1:200; Bio-Rad Cat #MCA1957) with secondary staining performed for 45 minutes RT using Alexa Flour 594 donkey anti-rabbit (1:400 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and incubated for 1 hour with fluorescent-tagged secondary antibody diluted in PBST (Goat anti-Rabbit IgG StarBright™ Blue 700 or Goat anti-Mouse IgG DyLight™ 800, Bio-Rad). Finally ...
-
bioRxiv - Immunology 2023Quote: ... The expression of immune system markers were mostly studied with inmunohistochemistry using the following antibodies: mouse anti rat CD68 (dilution 1:300 BIORAD, ref. MCA 341R) for monocytes/macrophages ...
-
eATP/P2X7R axis drives nanoparticle induced neutrophil recruitment in the pulmonary microcirculationbioRxiv - Immunology 2024Quote: ... Cytokines and chemokines in the blood and BALF samples were quantified following the manufacturer’s protocol for the Bio-Plex Pro Mouse Chemokine Panel 31-Plex (Bio-Rad Laboratories, California, USA). Data acquisition was performed using a LuminexTM 200 plate reader (InvitrogenTM ...
-
bioRxiv - Microbiology 2024Quote: ... The copy number of specific transcripts was determined by analysing mouse cDNA in a 10 μl reaction mix consisting of 1x iQ SYBR® Green Supermix (Bio-Rad, USA), 0.2 μM of each primer and 2 μl of template cDNA on a CFX Connect Real-Time System (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Serum hormones were quantified on a Luminex MAGPIX platform using the Bio-Plex Pro Mouse Diabetes 8-Plex and Adiponectin Assays (Bio-Rad, Hercules, CA) and the IGF-1 mouse Luminex Discovery Assay (R&D Systems ...
-
bioRxiv - Microbiology 2019Quote: ... Each PCR was performed in a total volume of 10 µL containing 5 µL of 2 × iQSYBR Green supermix (Bio-Rad, USA), 200 nM of each primer ...
-
bioRxiv - Molecular Biology 2021Quote: Amplification was carried out using PCR mixture-2 blue (AmpliSens, Russia) containing Taq-polymerase on a T100 Thermal Cycler (Bio-Rad, USA). Next ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 2% β-mercaptoethanol) and separated on a 10% gel by SDS-PAGE (TGX Stain-Free™ FastCast™ Acrylamide Solutions; BIORAD) or 4-15% gel (Mini-PROTEAN® TGX Stain-Free™ ...
-
bioRxiv - Genomics 2022Quote: ... Each sample was tested by PCR systems (threshold – 0,050) to the presence of SARS-CoV-2 using a CFX Connect Real-Time PCR System (BioRad, Hercules, California, USA). RT-PCR were implemented in 40 μl of final volumes ...
-
bioRxiv - Neuroscience 2019Quote: The membrane was washed in 1x Tris buffered saline with 0.1% Tween-20 (TBS-T) and blocked in TBS-T with 2% (w/v) blotting grade blocker (Bio-Rad, Cat#: 1706404). Membranes were incubated in primary antibodies (Table 2 ...
-
bioRxiv - Biochemistry 2021Quote: ... the reactant was mixed with the 2 × SDS-PAGE sample buffer containing 100 mM β-mercaptoethanol and run on 12% Mini-PROTEAN TGX Precast Gel (Bio-Rad) without a boiling step ...
-
bioRxiv - Microbiology 2021Quote: ... The amplified genomic DNA from the PPA strains was resolved using 2% agarose gel and visualized in the Gel DocTM XR+ documentation system (Bio-Rad, USA).
-
bioRxiv - Microbiology 2021Quote: ... aeruginosa isolates were separated in 2% agarose gel and visualized their banding patterns in the Gel DocTM XR+ documentation system (Bio-Rad, USA).
-
bioRxiv - Neuroscience 2021Quote: ... Samples were then loaded onto 4-15% Criterion™ TGX™ Precast Midi Protein Gels (12 + 2 wells, 45 μl) (Bio-Rad) and subjected to SDS-PAGE at 200 V for ~45 min in 10× Tris/Glycine/SDS running buffer (Bio-Rad) ...
-
bioRxiv - Neuroscience 2021Quote: ... Master mixes containing primer pairs against FIBCD1 or B2M (Beta-2-microglobulin, housekeeping gene) were prepared with iTaq Universal SYBR Green Supermix (Bio-Rad #1725122). The mastermixes were dispensed into each well containing cDNA and incubated on ice for 15 minutes to allow the cDNA to dissolve ...
-
bioRxiv - Plant Biology 2020Quote: ... Reactions were performed with 2 × Taq Master Mix (Vazyme, Nanjing, China) on a Bio-Rad Thermal Cycler (Bio-Rad, Hercules, CA, USA) using the following procedure ...
-
bioRxiv - Genomics 2021Quote: ... PCR products were resolved using a 2% agarose tris-acetate-EDTA gel and a 177 bp band was visualized using the ChemiDoc™ imaging system (Bio-Rad).
-
bioRxiv - Microbiology 2021Quote: ... 100 ng plasmid DNA and 100 μL of electrocompetent mycobacteria were mixed and transferred to a 2 mm electroporation cuvette (Bio-Rad #1652082). Where necessary ...
-
bioRxiv - Neuroscience 2020Quote: ... and followed by incubation with HRP-conjugated secondary antibodies for 2 hours at room temperature: goat anti-rabbit IgG (BioRad, #170-6515) or goat antimouse IgG (BioRad ...
-
bioRxiv - Plant Biology 2020Quote: ... or 1 μl (for the rest) of a 1/2 dilution of the cDNA was added to a reaction containing 1X of SsoFast EvaGreen (Bio-Rad, USA), 0.5 μM Forward and Reverse primers (Table 2) ...
-
bioRxiv - Microbiology 2020Quote: ... which subsequently performed 2-DE using a 7 cm immobilized pH gradient (IPG) strips (pH range of 3—10, Ready strip, Bio-Rad, USA).
-
bioRxiv - Cancer Biology 2021Quote: ... 60 of High Capacity NeutrAvidin slurry (Thermo PI29204) were washed three times with 1 mL 2X RIPA/1mM EDTA buffer in 2-mL chromatography columns (Bio-Rad 7326008) attached to a vacuum manifold (Promega A7231) ...
-
bioRxiv - Cell Biology 2021Quote: ... Selected siRNAs were spotted onto a gridded MatTek dish (P35G-2-14-C-GRID) using a contact spotter (ChipWriter Pro-Bio-Rad Laboratories) resulting in a layout of 4 × 8 spots 40 ...
-
bioRxiv - Microbiology 2022Quote: SARS-CoV-2 viral RNA loads were determined using Droplet Digital PCR (ddPCR) supermix for probes without dUTP (Bio-Rad Laboratories Ltd) and the QX200 Droplet Digital PCR System Workflow (Bio-Rad Laboratories Ltd) ...
-
bioRxiv - Neuroscience 2020Quote: Brain tissues were homogenized in lysis buffer and then combined with 2 x Laemelli buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Protein samples were then separated on 7.5 to 12.5% v/v sodium dodecyl sulfate (SDS ...
-
bioRxiv - Developmental Biology 2021Quote: ... following by 25 cycles of preamplification using mix of 47 pairs for MTE primers, each at final 50nM concentration (Supplementary information, Supplementary Table 2) and SsoAdvanced™ PreAmp Supermix (Cat# 1725160, Bio-Rad). After amplification ...
-
bioRxiv - Biochemistry 2021Quote: ... The purified proteins were running in 2-D gel electrophoresis and images were taken and analyzed in gel dock(Bio-Rad, USA). The details of protein purification protocols like TCA/Acetone ...
-
bioRxiv - Plant Biology 2021Quote: ... The pelleted nuclei were re-suspended in 200 µl of NIB and mixed with a 140 µl aliquot of melted 2% LMA agarose (Bio-Rad, 1703594) at 43°C ...
-
bioRxiv - Microbiology 2020Quote: ... Non-human primate samples were inactivated with γ-radiation (2 MRad) according to standard operating procedures and assayed on a Bio-Plex 200 instrument (Bio-Rad) using the Non-Human Primate Cytokine MILLIPLEX map 23-plex kit (Millipore ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were resuspended in MACS buffer (PBS +2% FBS +2mM EDTA) and counted using trypan blue with the TC20™ Automated Cell Counter (Bio-Rad). In some cases ...
-
bioRxiv - Neuroscience 2019Quote: ... and samples were then transferred at 100 V at 4°C onto an activated 2 um polyvinylidene difluoride (PVDF) membrane (Bio-Rad 1620177), blocked with 3% BSA in 0.2% Tween-20 (Sigma P9614 ...
-
bioRxiv - Neuroscience 2019Quote: ... The PCR product was confirmed by using 2% agarose gel electrophoresis and visualized under a Gel Doc system (BioRad, Hercules, CA, USA). The amplified products were purified with the QIAquick PCR purification kit (Qiagen ...
-
bioRxiv - Cell Biology 2019Quote: ... which was performed using a 2 μl cDNA /20 μl reaction volume on a CFX Connect™ real-time PCR detection system (Bio-Rad) using SYBR Green according to the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2019Quote: ... The mixed beads were washed once with the washing buffer and biotinylated proteins were eluted by 30 μL 2× SDS sample buffer and separated by SDS-PAGE (Biorad, Hercules, CA).
-
bioRxiv - Plant Biology 2020Quote: ... Incubate 1 μL of reticulocyte extract before binding with beads and 2 μL of extract after the binding was used for each western on a precast gel (4–20% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 μl, BioRad). Before loading ...
-
bioRxiv - Microbiology 2020Quote: Serum samples were inactivated with γ-irradiation (2 mRad) and cytokine concentrations were determined on a Bio-Plex 200 instrument (Bio-Rad) using Milliplex Mouse Cytokine/Chemokine MAGNETIC BEAD Premixed 25 Plex Kit (Millipore) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... 10 μL of reconstituted representative influenza vaccine (i.e., AddaVax/Y-2) of Fluad Quadrivalent was mixed with Laemmli Sample Buffer (Bio-Rad, Hercules, CA) and β-mercaptoethanol (2% ...
-
bioRxiv - Microbiology 2019Quote: ... and FLAG-cMCF-HA recovered as described above were prepped using the Bio-Rad ReadyPrep 2-D Cleanup Kit (Bio-Rad 1632130). The sample was then loaded onto 11 cm pH 3-10 immobilized pH gradient (IPG ...
-
bioRxiv - Neuroscience 2020Quote: ... brain tissues were homogenized in lysis buffer and then combined with 2 x Laemelli buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Protein samples were then separated on 7.5 to 12.5% v/v sodium dodecyl sulfate (SDS ...
-
bioRxiv - Molecular Biology 2019Quote: ... Aliquots of 50 μg protein in SDS-PAGE sample buffer containing 0.2 M dithiothreitol were heat-denatured for 5 min at 95 °C then electrophoresed on AnyKD midi criterion™ gels (Bio-Rad) in ProSieve™ EX running buffer (Lonza) ...
-
bioRxiv - Cancer Biology 2021Quote: ... according to the manufacturer’s instructions in a MyiQ™2 Two-Color Real-Time PCR Detection System (Bio-Rad, Veenendaal, The Netherlands).
-
bioRxiv - Cell Biology 2021Quote: ... 1∼2 μg of total protein for each lane) was loaded on a precast 4-20% gradient polyacrylamide gel (PAGE, Bio-Rad: 4561096) and electrophoresed at a constant voltage of 200 V for 35 min ...
-
bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were detected by incubation with HRP-conjugated secondary antibodies for 2 hours at room temperature: goat anti-rabbit IgG (BioRad, #170-6515) or goat anti-mouse IgG (BioRad ...
-
bioRxiv - Microbiology 2022Quote: ... Proteins were visualized with goat anti-mouse or goat anti-rabbit IgG secondary antibodies (1:5000) diluted in 2% Omniblot milk (AmericanBio) in 1X TBST using a chemiluminescence detection system (BioRad ChemiDoc MP).