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Citations for Bio-Rad :
1551 - 1600 of 1976 citations for MBL 2 Mouse HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The PCR product was then run on a 2% agarose gel and imaged with a ChemiDoc Gel Imager (Bio-Rad).
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bioRxiv - Developmental Biology 2021Quote: ... was performed using 1 μl of cDNA as a template with qPCR primers (Table 2) and iTaq Universal SYBR Green Supermix (Biorad) according to the manufacturer’s instruction and analyzed using CFX Connect Real Time System (BioRad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantification of percentage of exon 2 inclusion was performed with ImageJ or with ImageLab software (version 5.2, Bio-Rad Laboratories) when 32P-labelled DUP51S_F primer was used for the PCR ...
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bioRxiv - Cell Biology 2019Quote: ... An 11 kPa polyacrylamide (PA) solution (715 µL 50 mM HEPES pH 7.4, 150 µL 2% bis-acrylamide (Bio-Rad), 125 µL 40% acrylamide ...
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bioRxiv - Microbiology 2021Quote: ... The nitrocellulose strips were blocked in PBS with casein for 30 mins at room temperature and then probed for 1 hour with monoclonal antibodies (mAb) recognizing H3 HA (clone 36/2 (26)) or IAV M1 matrix protein (clone MCA401, BioRad). Strips were washed and incubated for 1 hour with a horse radish peroxidase (HRP)-conjugated secondary immunoglobulin (DAKO) ...
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bioRxiv - Developmental Biology 2019Quote: ... RT-PCR was performed with 10–100 ng of cDNA template in a 25 μl total reaction volume (12.5 μl iQ SYBR Green Supermix (2×) (Bio-Rad), 0.5 mM of each gene-specific primer ...
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bioRxiv - Molecular Biology 2020Quote: ... the fractions that contained RTs were pooled and loaded onto a 2-ml Macro-Prep DEAE Resin (Bio-Rad, USA) column pre-equilibrated with buffer C (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... and the supernatants were purified with IBA Strep-Tactin sepharose beads (IBA, 2-1201-002) on Poly-Prep Chromatography Columns (731-1550, BioRad) with IBA’s buffer’s W (washing ...
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bioRxiv - Cell Biology 2020Quote: ... The embryos were mounted on a 2 % agarose pad and heat-shocked at 30°C for 1h (Thermocycler Bio-Rad). After 2h recovery at 20°C ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... with modified primer lengths that allowed all six loci to be used in combination in a single PCR in a DNA Engine Tetrad 2 thermal cycler from BioRad set at the following parameters ...
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bioRxiv - Biochemistry 2021Quote: ... 2.5×106 Jurkat T cells were electroporated with 2 μg minigene constructs using 220 V and 1,000 mA (Gene pulser X,Bio-Rad). After 24 h incubation ...
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bioRxiv - Cancer Biology 2021Quote: Click reaction mixtures and unreacted alkyne-modified oligonucleotides were separated in a 1.2 % agarose gel for 1 h at constant 120 mV and DNA bands were captured by the ChemiDoc system (BioRad) using SYBR safe mode.
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bioRxiv - Immunology 2021Quote: For performing the digital droplet-PCR (ddPCR) we prepared the reaction mixture by combining a 2× ddPCR Mastermix (Bio-Rad), 20× primer ...
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bioRxiv - Biophysics 2021Quote: ... The solution was then transferred to a tube containing ∼30- 50 mg of Bio-Beads (Bio-Beads SM-2, BioRad) that were previously equilibrated in 20 mM Tris-HCl pH 7 ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were electrophoresed in a 2% agarose gel and visualized using a Bio-Rad gel system (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... Bradford Protein Assay Reagents 1 and 2 and Precision Plus Protein All Blue Prestained Protein Standard were from Bio-Rad. The anti His-tag mouse antibody was from GenScript (cat no ...
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bioRxiv - Immunology 2020Quote: ... Cathepsin B activity was determined using Magic Red™ (a cell-permeable and non-cytotoxic reagent that contains a cathepsin B target sequence peptide (RR)2 linked to a red (Cresyl Violet) fluorescent probe) and lysosomes were detected with acridine orange (Biorad), according to manufactures instructions.
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bioRxiv - Microbiology 2020Quote: ... coated with PBS alone or with 2 µg/ml of SARS-CoV-2 S protein in PBS overnight at 4°C were blocked for 1 hour with 3% nonfat dry milk (Biorad) in PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... 5 μL of PCR1 reaction (2 ng) were combined for the qPCR reaction using CFX 96 Touch Biorad qPCR thermocycler (Biorad). Δct was calculated by subtracting the mean Cq of each sgRNA to a non-targeting control ...
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bioRxiv - Genetics 2019Quote: ... Gels were transferred in buffer containing 20% methanol and 0.03% SDS for 2 hours at 4C at 28V onto 0.2μM nitrocellulose membranes (Bio-Rad, cat #1620097). Membranes were blocked in tris-buffered saline and Tween 20 (TBST ...
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bioRxiv - Immunology 2022Quote: ... 2 μm paraffin-embedded kidney sections were incubated with an anti-F4/80 antibody (CI:A3-1; Bio-Rad, Feldkirchen, Germany). After incubation with biotinylated goat anti-rat IgG (Abcam ...
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bioRxiv - Biochemistry 2022Quote: ... The other two lanes were incubated with 1xTris/glycine/SDS buffer for 15 min and then incubated with 2 x Laemmli sample buffer (BioRad) for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... and the nuclear ACTIN locus (Supplementary Table 2) were independently amplified from 12.5 ng of total genomic DNA with ssoAdvanced SYBR Green Supermix (Bio-Rad) in a total volume of 10 μl with standard cycling condition suggested by Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... before solubilisation in 2% SDS and subsequent OD595 measurements were obtained using an iMark microplate reader (Bio-Rad, Laboratories, Inc.). The number of adherent cells was claculated by the confluence in each concentration of CB-839/UK-5099 at 72 hours ...
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bioRxiv - Biochemistry 2022Quote: ... were exchanged from size exclusion buffer into 2 M ammonium acetate pH 7.4 via a single-pass through a Bio-Spin P-6 column (Bio-Rad). Buffer-exchanged protein was loaded into gold-coated glass capillaries (1.0 mm OD/0.75 mm inner diameter with filament) ...
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bioRxiv - Bioengineering 2022Quote: RNA was extracted as described above, followed by RT-PCR using the Allplex SARS-CoV-2 assay (SeeGene, South-Korea) on a CFX96 thermocycler (Biorad), simultaneously detecting four different target genes ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... The protein extract was solubilized in 2-D lysis buffer (30 mM Tris-HCl pH 8.8, containing 7 M urea, 2 M thiourea and 4% CHAPS. Protein concentration was measured using Bio-Rad protein assay method ...
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bioRxiv - Cell Biology 2022Quote: ... Approximately 75 islets were sandwiched in a chamber between two layers of Bio-Gel P-2 (Bio-Rad, Cat#1504118) bead solution (200 mg beads/ml in KRB buffer) ...
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bioRxiv - Plant Biology 2022Quote: ... Then 2 μl of 10 times diluted cDNA was used for CFX96 Touch Real-Time PCR Detection System (Bio-Rad) for Syber green based quantitative PCR analysis ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were washed (3 × 5min TBS-T and 2 × 5 min TBS) before being imaged on the ChemiDoc MP Imaging System (Biorad). Subsequently ...
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bioRxiv - Zoology 2022Quote: ... and folded by heating to 95°C for 2 min and slowly cooling down at 0.1 °C per second using a thermocycler (Bio-Rad). DsRNA concentrations were adjusted to 3 or 14 μg / μl using SpeedVac Concentrator (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The resulting cDNA was diluted 1:10 and 2 µL was used for qPCR amplification of SARS-CoV-2 spike using iTaq Universal SYBR Green Supermix according to the manufacturer’s protocol (Bio-Rad). Copy number was calculated in reference to a standard curve of known copy number (102 – 107 copies spike) ...
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bioRxiv - Molecular Biology 2022Quote: ... The combined solution was incubated at room temperature for 1 hr and then mixed with BioBeads SM-2 resin (BioRad) overnight to remove the detergents ...
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bioRxiv - Synthetic Biology 2022Quote: ... the mixture was transferred to ice-cold electroporation cuvettes with a 2 mm gap (Bio-Rad Laboratories GmbH, Germany, #1652086). Electroporation was performed using the MicroPulser Electroporator (Bio-Rad Laboratories GmbH ...
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bioRxiv - Synthetic Biology 2023Quote: ... and incubated for 24 h in a thermal gradient using a DNA Engine Tetrad 2 Peltier Thermal Cycler (Bio-Rad) with the lid set to 50°C ...
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bioRxiv - Microbiology 2022Quote: ... competent cells were mixed with 500 ng of DNA in electroporation cuvettes (2 mm gap) and the reactions were electroporated (2.5 kV) using a MicroPulser electroporator (Bio-Rad). Electroporated cells were recovered in 1 mL LB (unless otherwise stated ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates in Laemmli buffer were separated by electrophoresis on 12% SDS-PAGE gels and transferred for 2 hours at 4°C on to a PVDF membrane (BioRad). After blocking for an hour in 5% dry milk ...
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bioRxiv - Biophysics 2022Quote: Detergent removal and generation of proteoliposomes was achieved using polystyrene beads (Bio-Beads in SM-2 Adsorbent Media, BioRad, Inc.). The protocol was adapted and refined from 20 ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were subsequently blocked for 2 h at room temperature with 5% non-fat milk (Bio-Rad, Hercules, CA, USA) or 5% bovine serum albumin (BSA ...
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bioRxiv - Systems Biology 2023Quote: ... and total protein abundance was normalized to 2 μg using the Detergentcompatible Protein Assay (Biorad, Hercules, CA, USA, Cat. # 5000111). 2 μg α-RelA antibody (Cell Signaling ...
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bioRxiv - Immunology 2023Quote: ... Blocked membranes were incubated with HuCAL anti-bovine Mincle CRD antibodies (2.5 µg mL-1) before incubation with secondary goat-anti-human IgG F(ab’)2: HRP (STAR126P, Bio-Rad) antibody ...
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bioRxiv - Microbiology 2023Quote: ... 1.0 mg purified plasmid DNA was added to 0.2 ml aliquot of the above-prepared fusobacterial cells in a pre-cooled corvette (0.1 cm electrode gap, Bio-Rad) and left on ice for 10 minutes ...
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bioRxiv - Genomics 2023Quote: ... Gels were run in 1X tris-acetate SDS running buffer for 2 hr and transferred to a 0.45 μm PVDF membrane using the Turbo Trans-Blot transfer system (Bio-Rad) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... 1.0 µg purified plasmid DNA was added to 0.2 ml aliquot of the above-prepared fusobacterial cells in a pre-cooled corvette (0.1 cm electrode gap, Bio-Rad) and left on ice for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... The was filtered through a 0.45 μm filter and His-MSI-2 was purified with HisTrapTM FF column (Cytiva) using the Econo Gradient Pump (BIORAD) according to the manufacturer’s protocol and overnight dialysis ...
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bioRxiv - Microbiology 2023Quote: ... 10 min), centrifuged (1,000 x g, 2 min) and loaded on 10% or 4–20% SDS-PAGE gel (Bio-Rad). Following electrophoresis (60 min ...
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bioRxiv - Cell Biology 2023Quote: ... then the pellet was resuspended in 212 µL of Laemmli sample buffer with 2-mercaptoethanol added (Bio-Rad, Hercules, CA). To neutralize the residual TCA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the nitrocellulose membrane was incubated with ECL reagent SignalFire for 1-2 min and imaged using a ChemiDoc (Bio-Rad).
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bioRxiv - Cancer Biology 2022Quote: ... 95°C for 2 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min (BioRad). The following primers from Integrated DNA Technologies (IDT ...
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bioRxiv - Biochemistry 2022Quote: ... The PVDF membranes were then incubated with HRP-coupled secondary antibodies (1:10,000, CST) for 2 h at 25°C and visualized by ChemiDoc XRS (Bio-Rad) instrument.