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Citations for Bio-Rad :
1451 - 1500 of 2091 citations for MBL 2 Mouse HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... and Mouse IgG HRP-linked whole Ab (Cytiva), then imaged using (ECL Select, Cytiva) with a ChemiDoc MP (Bio-Rad). For gel quantification ...
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bioRxiv - Immunology 2023Quote: ... and IFNγ) by the Bio-Plex Pro Mouse Cytokine Assay kit with the Bio-Plex 200 systems from Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with primary antibodies (mouse anti-human Aβ 82E1 [1:500, IBL 27725], rat anti-CD68 [1:250, BioRad MCA1957] ...
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bioRxiv - Genetics 2023Quote: ... Blots were washed in 1x TBST with mild agitation before incubation with horseradish peroxidase-conjugated secondary antibodies diluted in blocking buffer (α- mouse 1:10,000 (170-6516; BioRad), α-rabbit 1:10,000 (170-6515 ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were visualized using the secondary antibody Anti-Mouse IgG-HRP using Clarity Max™ Western ECL Substrate (BioRAD).
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Effects of menopause and high fat diet on metabolic outcomes in a mouse model of Alzheimer’s diseasebioRxiv - Neuroscience 2023Quote: ... Diabetes-associated markers were assessed using the Bio-Plex Pro Mouse Diabetes 8-Plex Assay (171F7001M; Bio-Rad, Carlsbad, California) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Additional antibodies used for the infected ALI cultures include: mouse anti-RSV Fusion protein (1:100, Cat# MCA490, Bio-Rad), rabbit anti-Cleaved Caspase-3 (Asp175 ...
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bioRxiv - Neuroscience 2024Quote: ... The blot was washed 3 times with TBST for 5 minutes and was incubated with 10 mL of 3% BSA/TBST (w/v) with 1 µL anti-mouse-HRP secondary antibody (1:10,000; 170-6516, Bio-Rad) for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was incubated with secondary antibody diluted 1:7500 in blocking buffer (anti-rabbit, anti-mouse HRP, Bio-Rad). Signal was detected using Pierce ECL Western Blotting Substrate (Fisher 32106) ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were then incubated in primary antibodies at 4°C overnight (1:500, rat anti-mouse CD68, Bio-rad Cat ...
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bioRxiv - Immunology 2024Quote: ... Lysates were then cleared by centrifugation for 15 minutes at 14,000 rpm at 4°C and subjected to immunoprecipitation with anti-V5 mouse monoclonal antibody (#MCA1360, Bio-Rad) overnight at 4°C with rotation in DNA/RNA LoBind tubes ...
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bioRxiv - Physiology 2024Quote: ... The secondary antibodies were goat anti-mouse HRP-linked and goat anti-rabbit HRP-linked (Bio-Rad, Hercules, CA, USA) for immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... were harvested and resuspended in 1X PBS-NaN3 (0.2%) and embedded in 2% low-melt agarose (Mb grade, BioRad). Agarose plugs were chilled at 4°C for 30 min and then ejected into Proteinase K digestion buffer (1 mg/mL proteinase K ...
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bioRxiv - Biophysics 2022Quote: ... Nanodisc reconstitution was induced by removing detergent with 0.8 mg ml-1 pre-washed Biobeads SM-2 (Bio-Rad) for 2 hours with gentle agitation at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were electroporated in 2-mm cuvettes (600 V, 50 μF, 200 Ω) by using Gene Pulser Xcell (Biorad). Immediately after electroporation ...
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bioRxiv - Molecular Biology 2021Quote: ... resolved by size on a 2% agarose gel and imaged on Gel Doc XR+ and ChemiDoc XRS+ systems (Biorad).
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bioRxiv - Cell Biology 2022Quote: ... The reaction mixture (total volume 22 μl) contained 11 μl of 2× ddPCR Super Mix for Probes (BioRad, 1863024), 1.1 μl of 4 primers mixture 18 μM each ...
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bioRxiv - Pathology 2019Quote: ... by SDS-PAGE at 100 V for 2 hours in a Mini-PROTEAN® Tetra cell apparatus (Bio-Rad). The Kaleidoscope commercial standard Precision Plus Protein Standard (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... Reverse Transcription cDNA synthesis reactions were performed on 0.2 μg −2 μg total RNA with iScript cDNA synthesis kit (BioRad) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2019Quote: ... The cDNA was then diluted 10x with water and 2 µl was mixed with iQ SYBR Green Supermix (BioRad) containing 100 nM of forward and reverse primers ...
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bioRxiv - Genetics 2020Quote: ... 2 mM ATP and 1 mM DTT and the reactions were stopped by addition of Laemli sample buffer (BioRad). Samples were run on precast 4-20% gradient SDS-PAGE gels (BioRad ...
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bioRxiv - Immunology 2021Quote: ... Quantitative PCR was performed in 20 μL reactions in triplicate containing 10 μL 2 × SsoFast EvaGreen Supermix (Bio-Rad,), 1 μL of primers (3 μM F and 3 μM R) ...
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bioRxiv - Microbiology 2021Quote: ... the plates were sealed and incubated at 37°C for 2 h in a T100 Thermal Cycler (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: Resuspended purified beta-propiolactone treated SARS-CoV-2 virus preps were separated by SDS-PAGE (Bio-Rad TGX Mini) and transferred to 0.2 µM PVDF membrane according to the manufacturer’s protocols (Bio-Rad Tansblot Turbo) ...
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bioRxiv - Cancer Biology 2021Quote: Protein lysates from SARS-CoV-2 infected Vero E6 cells were harvested in 2x Laemmli sample buffer (Bio-Rad) containing 2-Mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... 150 mM KCl and 2 mM MgCl2) at 37°C for 16 hours in thermal cycler (Bio-Rad, USA). The samples were separated using 10% non-denaturing polyacrylamide gel and tris/boric acid/EDTA buffer (TBE ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR products were run on 2% agarose gel and visualized in a Gel Doc EZ System (Bio-Rad, USA). GAPDH served as an endogenous control.
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bioRxiv - Biochemistry 2021Quote: ... The protein was incubated at 4°C for 1 h with Bio-Beads™ SM-2 resin (Bio-Rad) equilibrated with 100 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... and 30μg of total protein per sample was mixed with Laemmli buffer supplemented with 2-mercaptoethanol (10%) (Bio-rad) and boiled at 99°C for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... culture supernatants were collected and cells were directly lysed with 150 μl of 2× laemmli buffer (Bio-rad, 1610737). Proteins in culture supernatants and cell lysates were separated by SDS-PAGE and transferred onto PVDF membranes by using the Trans-Blot Turbo system (Bio-rad) ...
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bioRxiv - Biochemistry 2020Quote: ... 10 µL of ∼500 nM RNA was mixed with 2 µL of RNA loading dye and loaded into a 1.0 cm well PAGE casting plate (BioRad). Urea-PAGE (11.2% ...
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bioRxiv - Cell Biology 2020Quote: ... differentiated organoids and differentiated monolayers) and was calculated using the 2-ΔΔCt method using CFX Maestro software (Bio-Rad). Transcription was normalised to the expression levels of the housekeeping gene (18S ribosomal RNA ...
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bioRxiv - Plant Biology 2020Quote: ... Each qRT-PCR reaction of CZ and ZC was performed by the manufacturer’s instructions of EvaGreen Express 2×qPCR MasterMix (abm, Cat. No.MasterMix-ES, http://www.abmGood.com/) and BioRad®CFX96 Real-Time PCR system (Bio-Rad). Relative expression was quantified with the geometric mean of internal reference genes ETIFI (eukaryotic translation initiationfactor 1) ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 detection was performed on the CFX96™ Real-Time PCR Detection System (Bio-Rad, California, USA), using the NeoPlex™ COVID-19 Detection Kit (Genematrix ...
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bioRxiv - Cancer Biology 2021Quote: ... according to the manufacturer’s instructions in a MyiQ™2 Two-Color Real-Time PCR Detection System (Bio-Rad). The threshold for detection of fluorescence was manually adjusted to 40 Relative Fluorescent Units to obtain the same threshold for each plate ...
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bioRxiv - Cell Biology 2020Quote: ... Total numbers of viable cells were determined after 2 and 4 days using the TC10 cell counter (Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR reactions were conducted using 10 µL 2× SYBR-Green Universal Master Mix (Bio-Rad, Hercules, CA, USA), 2 µL of forward primer (9 mM) ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were incubated at the adequate temperature during 2-3 days and photographed with the Chemiluminiscent Imager (Bio-Rad). For methyl methanesulfonate (MMS ...
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bioRxiv - Immunology 2021Quote: ... Bound PRRSV-2-specific IgG was detected using goat anti-pig IgG conjugated with horseradish peroxidase (HRP; Bio-Rad) diluted at 1:10,000 in blocking buffer for 1 h at RT ...
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bioRxiv - Immunology 2021Quote: Frozen PBMCs were thawed, washed in FACS buffer (2% FBS, 1mM EDTA in PBS) and counted on TC20 (Biorad) before surface staining using the following panel ...
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bioRxiv - Biophysics 2022Quote: ... 2 μL of cDNA was mixed with 2 μL of forward and reverse primers (2.5 μM each, Table S1,) and 1x SYBR Green Supermix (BioRad) for a final reaction volume of 20 μL ...
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bioRxiv - Cancer Biology 2022Quote: ... and protein levels were quantified using BCA Assay (Pierce) and supplemented with 4x Laemmli Buffer with 2-mercaptoethanol (Biorad). Samples were boiled and sonicated ...
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bioRxiv - Microbiology 2022Quote: ... Virus was then inactivated and whole cell lysates taken by the addition of 2× Laemmli buffer (Bio-Rad #161073) + 2-mercaptoethanol (Bio-Rad #1610710 ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells:DNA mixture was transferred to an ice-cold 2 mm gap electroporation cuvette (Molecular Bioproducts) and electroporated at 2.5 kV with a MicroPulser (BIORAD). One ml of Super Optimal broth with Catabolite repression (SOC ...
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bioRxiv - Plant Biology 2022Quote: ... Each RT-qPCR reaction (10 μl) included 2 × iTaq™ Universal SYBR®Green Supermix (Bio-Rad, CA, USA), 500 nM forward and reverse primers (Supplementary File 1C) ...
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bioRxiv - Molecular Biology 2022Quote: ... The SARS-COV-2 NTD expression was analysed with SDS-PAGE followed by Western-blot on PVDF membrane (Biorad) using α-strep-tag mouse antibodies 1:3000 (IBA Life Sciences) ...
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bioRxiv - Microbiology 2023Quote: ... 2 min at 72°C for final extension in a T100 Thermal Cycler (BIO-RAD Lab. Inc., Hercules, CA). The sizes of PCR products were checked by running agarose gel electrophoresis ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 µL of cDNA was added to 10 µL of Bio-Rad ddPCR Supermix for Probes (1863024; Bio-Rad), followed by the addition of 1 µL of TaqMan miR-146a-5p 20X primer mix (4427975 ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 2% w/v CA and treated with (7% w/v) Chelex100 resin (Bio-Rad, Mississauga, ON, Canada) at 4°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... 2 % fetal bovine serum and 8 % chelated fetal bovine serum using Chelex® 100 Resin (BioRad, Ref. 142-1253). To differentiate HaCaT cells ...