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Citations for Bio-Rad :
1751 - 1800 of 8520 citations for 7 hydroxy 1H 1 2 4 triazolo 1 5 a pyrimidin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... TGN46 (Biorad, AHP500GT, 1:200), washed 3 times with PBS and incubated in blocking buffer for 45 min with fluorescently labelled secondary antibodies at 1:400 ...
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bioRxiv - Immunology 2023Quote: ... F480-PB (CI:A3-1)(BioRad), (v ...
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bioRxiv - Cancer Biology 2023Quote: ... in 1×TBS (BioRad #1706435)/0.1% Tween 20 (Bio-Rad #161-0781 ...
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bioRxiv - Cancer Biology 2023Quote: ... #0500-0099 (1/50000, Biorad). Proteins were enhanced by chemiluminescence reagent (Biorad ...
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bioRxiv - Immunology 2023Quote: ... (CD68, 1:200, BioRad, MCA1957T) and (IgA ...
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bioRxiv - Cell Biology 2024Quote: ... CD68 (Biorad MCA1957GA; 1:200), CD206 (Abcam ab64693;1:200) ...
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bioRxiv - Microbiology 2024Quote: ... TGN46 (BioRad, AHP500, 1/100), YBX1 (ProteinTech ...
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bioRxiv - Immunology 2020Quote: Staining of 2×105 cells per well was performed with mouse anti-human CD79α (clone HM57, Bio-Rad AbD Serotec, Puchheim, Germany, 1:100) for identification of B cells and Alexa Fluor 647-conjugated rat anti-human CD3∊ (clone CD3-12 ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216, Anti-FLAG; 1:2000 dilution, Sigma-Aldrich #F1804, anti-β-Tubulin; 1:1000 dilution, Bio-Rad #12004166). Then membranes were washed with 1X TBST 3 times (10mins each wash ...
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bioRxiv - Plant Biology 2023Quote: ... followed by addition of primary antibodies at 4 °C overnight with rotation (mouse anti-ϕ3-actin [Proteintech, 66009-1-Ig], 1:1000, and rat anti-χξ-tubulin [YOL1/34, MCA78G, Bio-Rad], 1:1000). The specimen was washed three times with PBST and incubated with secondary antibodies (anti-mouse ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were determined by BCA protein assay (Pierce) and boiled for 5 minutes with Laemmli Sample Buffer (Bio-Rad, Hercules, CA). Proteins were separated on acrylamide gels and analyzed by Western blot using primary antibodies ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were then washed 3 times for 5 min in TBST before detection using Clarity Western ECL Substrate (Bio-rad, 170-5061) and imaging on a ChemiDoc MP (Bio-rad ...
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bioRxiv - Neuroscience 2021Quote: ... were mixed with filtered monomeric α-syn WT (30 μM) and ThT (10 μM) and were incubated at 55°C for 5 h in a PCR machine (Bio-rad, C1000) for amplification.[3] Fractions comprised of ≥ 76% of its original volume were mixed with α-syn and ThT to reach a final volume of 20 μL per reaction ...
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bioRxiv - Molecular Biology 2020Quote: ... for 5 minutes and then transferred onto a protran BA-85 nitrocellulose membrane using a semi-dry transfer apparatus (Biorad 170-3940) for 75min at 400mA (not exceeding 15V) ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% β-mercaptoethanol and 10 mM Tris-HCl (pH 7.0)) and added sample loading buffer (Biorad XT sample buffer, Biorad Munich, Germany). The samples were separated on a 12% on a 12% gradient XT precast Criterion gel using XT-MOPS buffer (Biorad ...
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bioRxiv - Molecular Biology 2020Quote: ... carried out in glass tubes filled with polyacrylamide gel using a mixture of ampholytes 3/10 and 5/8 (Bio-Rad, USA) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: 5 μg of cheRNA or total RNA for each condition were denatured at 95°C for 5 min and transferred to a Hybond-XL membrane (Amersham) in a Bio-Dot® Microfiltration Apparatus (Bio-Rad) following manufacturer’s intructions ...
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bioRxiv - Synthetic Biology 2019Quote: First-strand synthesis was generated by mixing 5 µL of separated RNA transcript with 500 nM of reverse primer in a 20 µL reverse transcription reaction (Bio-Rad, 1708897) containing 4 µL of reaction supermix ...
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bioRxiv - Microbiology 2019Quote: Pre-cultures were made from calibrated explants (5 mm) deposited on plate with solid Sisler medium previously covered with a sterile cellophane membrane (Bio-Rad, USA). The cultures were maintained 4 days at 20°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Electrofocusing was performed in glass capillaries with polyacrylamide gel using a mixture of ampholytes 3/10 and 5/8 (#163-1112, #163-1192, Bio-Rad, USA). The second direction is standard SDS 5-10% PAGE followed by staining with Coomassie Brilliant Blue G-250 (#31-4-58-1 ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... 10μl of EMSA binding reactions were loaded on 5% nondenaturing polyacrylamide Mini-PROTEAN TBE gels (Catalog #4565015, Bio-Rad Laboratories, Hercules, CA) for electrophoretic analyses ...
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bioRxiv - Biochemistry 2020Quote: ... protein was eluted from the beads by boiling for 5 minutes at 95°C in Laemmli Sample Buffer (BioRad, catalog #161-0747). Proteins were stored at −80°C until western blotting.
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bioRxiv - Cell Biology 2021Quote: ... 25 ng of cDNA template was mixed with the primers to final concentrations of 200 nM and mixed with 5 μl of iTaqTM Universal SYBR® Green Supermix kit (Bio-Rad). The reactions were done in triplicates with the following conditions ...
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bioRxiv - Cell Biology 2021Quote: ... except that blocking was done with phosphate-buffered saline plus 0.1% Triton X-100 (PBS-T) containing 5% nonfat dry milk (Biorad Blotting grade Blocker) and 10 mM NaN3 ...
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bioRxiv - Microbiology 2021Quote: ... and 5 µl of PowerUp™ SYBR™ Green Master Mix (2X) using a CFX Connected Real-Time PCR machine (Bio-Rad). Primers used in this study were listed in Table S3 ...
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bioRxiv - Biophysics 2020Quote: ... the RNC samples from the above sucrose cushions were heated at 55 °C for 5 min in the presence of 1X Laemmli Sample buffer (Bio-Rad, 1610737). Subsequently ...
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bioRxiv - Developmental Biology 2022Quote: ... was added to 20 µg protein and samples were boiled at 95°C for 5 minutes before being loaded onto a 10% SDS-polyacrylamide gel (Bio-Rad #4568034). Protein was transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked for 1 h at room temperature (RT) in Tris-buffered saline with 0.05 % Tween-20 (TBST) containing 5 % blotting-grade dry milk (Biorad cat# 170-6404), incubated overnight with primary antibody (dilutions above mentioned ...
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bioRxiv - Immunology 2020Quote: ... Proteins were denaturated at 95°C for 5 minutes before separation on SDS-PAGE TGX 12% Stain-Free FastCast Acrylamide (Bio-Rad Laboratories). Proteins were then electro-transferred onto Trans Blot Turbo polyvinylidene fluoride membranes (Bio-Rad Laboratories) ...
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ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... and the corresponding 5 μl of Primer Master-mix was added followed by sealing the plate with Microseal ® adhesive sealer (Bio-Rad). The 60S acidic ribosomal protein P0 (rplp0) ...
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bioRxiv - Genomics 2020Quote: ... 5 µL of cDNA was pre-amplified in a 12-cycle PCR reaction using the Sso Advanced PreAmp Supermix (Bio-Rad, USA) in a 50 µL reaction ...
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bioRxiv - Developmental Biology 2022Quote: ... lieberkuehni developmental stages were mixed in 2x Laemmli sample buffer with 10 % 2-β-mercaptoethanol and heated at 97 °C for 5 min before loading on a 12 % (w/v) TGX FastCast SDS/PAGE gel (Bio-Rad, USA). Membranes were blocked in 3 % (w/v ...
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bioRxiv - Microbiology 2022Quote: ... burgdorferi 16S rRNA by preparing samples in a 96-well plate using 5 µL of 2X SYBR Green Master Mix (Bio-Rad #1725121), 1 µL of each primer (Forward ...
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bioRxiv - Bioengineering 2022Quote: ... that consists of 1 mL peroxide and 1mL luminol enhancer were spread onto membrane and left for 5 minutes before observation using ChemiDoc imager (Bio-rad Laboratories).
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bioRxiv - Epidemiology 2019Quote: ... digested DNA with SmaI was electrophoresed in 1% agarose gels for 21 hours with a ramped pulse time of 5 to 40 seconds using a CHEF DRII system (Bio-Rad Laboratories), using S ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Each PCR reaction (10 μL final volume) contained 5 μL SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad Laboratories, Hercules, CA), 100 ng of cDNA ...
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bioRxiv - Microbiology 2019Quote: ... The mixture was loaded in a chilled 0.1-cm cuvette with plasmid DNA and subjected to two square pulses at 5 s intervals using the Gene Pulser Xcell (Bio-Rad Laboratories) set at 0.55 kV voltage ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by 5 s at 95°C and 30 s at 60°C for 40 cycles using an Biorad CFX384 system (Biorad, Hercules, CA). The mRNA levels of each gene were normalized relative to the mean levels of the two housekeeping genes and compared with the data obtained from unstimulated ...
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bioRxiv - Physiology 2021Quote: ... the membranes were rinsed three times in TBS-T for 5 min and the proteins were visualized by enhanced chemiluminescense (BioRad, Clarity 1705061) in a BioRad Universal III Hood with Image Lab software v.6.0.1 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentrations were determined by BCA protein assay (Pierce) and boiled for 5 minutes with Laemmli Sample Buffer (Bio-Rad, Hercules, CA). Proteins were separated on acrylamide gels and analyzed by Western blot using primary antibodies ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were incubated with TBST-Blotto buffer (50 mM Tris-pH 7.5, 150 mM NaCl, 0.1% Tween-20, 5% w/v Bio-Rad Blotting Grade Blocker) for 1 h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... and 40ng of RNA for lung or 5-10ng of RNA for cells was converted to cDNA with iScript (Biorad, Hercules, CA). Real-time PCR was done on the cDNA with SYBR green master mix (Biorad ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then centrifuged (2500 × g for 5 min) and pellets were resuspended in 100 μL of PAGE Laemmli Sample Buffer (Bio-Rad, 1610747) mixed with 50 μL TE 1X pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... around 400 ng of protein was loaded in each lane and 5 μL of Precision Plus Protein™ All Blue Prestained Protein Standards (Biorad, USA) was loaded in each gel as a marker ...
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bioRxiv - Cancer Biology 2022Quote: ... 5’ - GAT GGA GAA AGC TCT GAG CAT C -3’ Reverse: 5’ - TTG CTC CAC AGA TGG AGT TG -3’ RHAMM PrimePCR primers were obtained from BioRad (Cat#: 10025636)
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was applied to a 5 ml MBPTrap HP Column (Cytiva, Marlborough, MA, USA) using an FPLC system (Bio-Rad NGC), pre-equilibrated and washed with 10 CV of buffer “A” ...
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bioRxiv - Immunology 2022Quote: ... Oligo-dT first strand cDNA synthesis was performed from 5 μg total RNA with iScript Advance cDNA Synthesis Kit for RT-qPCR (Bio-Rad; #1725038) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... The blot was then washed in 6x 5 minutes of TBST prior to being developed with Clarity™ Western ECL Substrate kit (Bio-Rad). To examine actin levels as a loading control blot was stripped using Restore™ WesternBlot Stripping Buffer (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 25-40μg of protein was run on 7.5-15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel and transferred using the Trans-Blot Turbo system (Biorad, Hercules, CA) to nitrocellulose membranes ...
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bioRxiv - Biochemistry 2022Quote: ... was loaded and electrophorized with a standard of 5□μl of Precision Plus Protein™ All Blue Prestained Protein Standards (BioRad #1610373). For electrophoresis ...