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Citations for Bio-Rad :
1901 - 1950 of 8520 citations for 7 hydroxy 1H 1 2 4 triazolo 1 5 a pyrimidin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... followed by a final extension step at 68 °C for 5 min on the T100™ thermal cycler (Bio-Rad, Hercules, CA, USA). During protocol optimization ...
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bioRxiv - Microbiology 2022Quote: ... Expression and secretion samples were boiled for 5 min and then resolved on Mini-PROTEAN or Criterion™ TGX Stain-Free™ precast gels (Bio-Rad). For immunoblotting ...
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bioRxiv - Immunology 2022Quote: ELISA plates were coated with 50 µL/well of 5 µg/mL of mouse anti-bovine IFN-γ monoclonal antibody (mAb) (CC330, Bio-Rad Antibodies) by overnight incubation at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... and 20 μL of the sample was mixed with 5 μL of a 5X Laemmli sample buffer containing 10% β-mercaptoethanol (Bio-Rad Laboratories). The sample was boiled for 5 min at 95 °C and loaded into a 12% polyacrylamide gel ...
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bioRxiv - Neuroscience 2023Quote: 1 µg BH RNAs and 5 µl bdEV RNAs were used to generate cDNA by iScript ™ Reverse Transcription Supermix for RT-qPCR (Bio-Rad 1708840). Expression levels of circRNAs were evaluated by qPCR using iTaq Universal SYBR ® Green Supermix (Bio-Rad 1725120) ...
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bioRxiv - Physiology 2023Quote: ... 50µL from this mixture was transferred to a new tube containing 950µL of PBS and stored at 5°C for subsequent the cell counting (using a TC20 Automated Cell Counter, Bio-Rad, Solna, Sweden) 10 to 30 min later (when the mitochondrial measurements had started) ...
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bioRxiv - Microbiology 2023Quote: ... The samples were loaded onto Any kD™ Mini-PROTEAN® TGX™ gels and separated under reducing conditions for 5 minutes at 250 V (Biorad). Proteins were visualized by Coomassie Blue Staining (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... heated in water bath for 5 minutes at 99°C and analysed by SDS-PAGE using Any-kD Mini-PROTEAN TGX Stain-Free gels (Bio-Rad, California, USA) in a 2-minute run for sample clean-up purposes ...
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bioRxiv - Physiology 2023Quote: ... heated (70°C for 5 min) and loaded (10 µg per lane) onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA). Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min ...
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bioRxiv - Biochemistry 2023Quote: Standard protocols were adopted for SDS-PAGE in 10% isocratic or 5%-15% gradient polyacrylamide mini-gels (Bio-Rad Laboratories S.r.l., Segrate, Italy), and for Western blotting by electro-transferring proteins from gels to PVDF membranes (0.2 μm pores ...
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bioRxiv - Plant Biology 2023Quote: ... reactions were prepared in a 10 µL final volume containing 5 µL of 2x SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad), 0.125 µL of each specific primer pair at 250 nM ...
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bioRxiv - Cell Biology 2023Quote: ... denaturated at 95 °C for 5 min and separated on Bis-Tris polyacrylamide gels using the Mini-PROTEAN® Tetra Handcast System (Biorad, cat.no. 1658029FC). For visualization of total protein load ...
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Post-stroke rapamycin treatment improves post-recanalization cerebral blood flow and outcome in ratsbioRxiv - Neuroscience 2023Quote: ... 50 µg of protein was denatured (95°C for 5 minutes) using lysis and Laemmli sample buffer (161-0737, Bio-Rad, with DTT). Samples were separated on a 10 % gradient gel (Criterion TGX Precast Gel ...
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bioRxiv - Neuroscience 2023Quote: ... and 5 U/mL aprotinin) and the protein concentrations in the lysates were determined using a BioRad protein assay kit (BioRad Laboratories, Hercules, CA). Protein samples were subjected to SDS-PAGE ...
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bioRxiv - Developmental Biology 2024Quote: ... and the reactions were stopped by boiling them at 95°C for 5 minutes after adding Laemmli sample buffer (Bio-Rad, 161-0747) supplemented with β-mercaptoethanol (Gibco 21985023) ...
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bioRxiv - Neuroscience 2024Quote: ... The slides were then incubated for 60 minutes at room temperature in blocking buffer (PBS with 5 % goat serum [Abcam #ab7481] and 0.3 % Triton X-100 [Bio-Rad Laboratories #161-0407]). After removing the blocking buffer and re-applying PAP pen ...
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bioRxiv - Molecular Biology 2024Quote: ... The gels’ stain-free dye was activated by a 5-min UV exposure and protein was transferred to Immun-Blot low-fluorescence PVDF membrane (Bio-Rad #162-0264) using Hoefer TE77X ...
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bioRxiv - Cell Biology 2024Quote: ... boiled for 5 min at 95° and resolved on 10 % or 7.5 % Mini-PROTEAN TGX Precast Gels (Bio-Rad, 4561033, 4561036, 4561023, 4561026). The SDS-PAGE was performed at 100 V for 5 minutes and then 120 V for 1 hour 15 minutes using SDS running buffer (30 g Tris ...
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bioRxiv - Biochemistry 2023Quote: ... and incubated at 4 °C for 30 min and heated at 37 °C for 5 min prior to analysis on precast “any KD” Mini-PROTEAN TGX (Bio-rad, Cat #4569033) polyacrylamide gels using 1x TBE as running buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of protein (25 µg) were denatured 1:1 in 2X Laemmli buffer (BIO-RAD) at 95°C for 10 minutes then held on ice ...
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bioRxiv - Pathology 2021Quote: ... Protein samples were first diluted 1:1 with Laemmli sample buffer (Bio-Rad, Hemel Hempstead, UK) containing 5% β-mercaptoethanol (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... followed by secondary antibodies (1:4000 donkey anti-goat, Abcam; 1:4000 goat anti-mouse, Biorad). Immunoblots were developed using SuperSignal West Pico PLUS Chemiluminescent Substrate (ThermoFisher).
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bioRxiv - Microbiology 2021Quote: ... 20 uL cell suspension was diluted 1:1 with 0.4% Trypan Blue solution (Bio-Rad #1450021) and cells were counted by hemocytometer (RPE-1 cells ...
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bioRxiv - Neuroscience 2021Quote: ... Each compartment of each sample was diluted 1:1 in Laemlli Sample Buffer (BioRad, Hercules, CA) mixed with 350mM DTT (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... The following antibodies and dilutions were used: F4/80 1:100 (C1:A3-1, Bio-RAD), αSMA 1:100 (AS-29553 ...
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bioRxiv - Microbiology 2022Quote: ... 100 μl fresh urine and several dilutions (1/10; 1/100) were plated on UriSelect4 (Biorad) and KFS (Biolife italiana ...
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bioRxiv - Plant Biology 2023Quote: ... 0.3% (w/v) CHAPS containing protease inhibitors (1 tablet Biorad/10ml ; 1/100 v/v PMSF). Phosphatase inhibitors were also added in L3 and L4 experiments ...
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bioRxiv - Physiology 2022Quote: ... monoclonal rat anti-F4/80 antibody (dilution 1:200) (clone Cl:A3-1, MCA497G, Biorad Laboratories, Germany) After multiple washes ...
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bioRxiv - Cell Biology 2022Quote: ... in 4°C for 2-4 hours and the beads were then isolated and eluted through a Bio-Spin® Chromatography Column (Bio-Rad, #7326008) with elution buffer (5 0mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate/bead mixtures were incubated at 4°C on a rotating platform for 2 hrs and subsequently poured into Poly-Prep® Chromatography Columns (Bio-Rad #7311550). Unbound fractions were collected through the columns and stored at −20°C ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... The resulting mixtures were dialyzed (20 kDa cutoff) for ∼18h at 4°C in the dark against 250 volumes of FB160M1 containing 2 g BioBeads SM2 (Bio-Rad, Hercules, CA) per 2 mL of RPL mixture ...
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bioRxiv - Microbiology 2020Quote: ... denatured at 95°C for 2 min and amplified for 24 cycles (95°C 15 seconds, 60°C, 4 min) on T100 PCR instrument (Biorad, Hercules, CA, USA). At the end of this process ...
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bioRxiv - Biochemistry 2020Quote: ... 8 % SDS-polyacrylamide (Biorad 29:1) gels were used for the full-length protein and 10 % SDS-polyacrylamide gels were used for the 95 aa reporter constructs ...
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bioRxiv - Molecular Biology 2020Quote: ... TGN46 at 1:1000 (Bio-Rad), for two hours at RT ...
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bioRxiv - Neuroscience 2020Quote: ... BrdU (Rat; 1:500; Bio-Rad). Alexa Fluor 488- ...
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bioRxiv - Neuroscience 2021Quote: ... anti-CD68 (1:500, Bio-Rad), and anti-Iba1 (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1 software (Bio-Rad, Hercules, USA).
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bioRxiv - Developmental Biology 2019Quote: ... F4/80 (1:1000, MCA497R, Biorad), MHC II (1:500 ...
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bioRxiv - Neuroscience 2019Quote: ... anti-CD11b (1:400, BioRad, MCA74G), anti-F8/40 (1:100 ...
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bioRxiv - Neuroscience 2019Quote: ... anti-CD68 (1:400, BioRad, MCA1957), anti-CD11b (1:400 ...
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bioRxiv - Neuroscience 2019Quote: ... anti-CD13 (1:50, BioRad, MCA2183), anti-CD68 (1:400 ...
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bioRxiv - Developmental Biology 2019Quote: ... BrdU (Bio-Rad MCA2060, 1:500), Ki67 (Abcam ab16667 ...
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bioRxiv - Immunology 2021Quote: ... NP (BioRad Cat#MCA400, 1:1000), and anti-β-actin (Sigma Aldrich Cat #A2228-100UL ...
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bioRxiv - Bioengineering 2021Quote: ... CD68 (1:100, MCA341R; Bio-Rad), and CD163 (1:400 ...
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bioRxiv - Bioengineering 2020Quote: ... mouse CD68 (Biorad, 1:200, MCA341GA), mouse CD45R (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... or anti-mouse (1:5000, BioRad). Stained membrains were developed with SuperSignal West Pico chemo luminescent substrate ...
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bioRxiv - Neuroscience 2021Quote: ... anti-CD68 (1:100; Biorad; MCA1957), anti-MPO (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... or Collagen-1 (1310001, Bio-Rad). Cells were then washed 3 times with PBS-T ...
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bioRxiv - Cancer Biology 2021Quote: ... β-Actin (Bio-Rad 12004163, 1:10,000) was used as a loading control ...
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bioRxiv - Cell Biology 2022Quote: ... anti-NBS1 (BioRad, VMA00403, 1:1000), anti-MDC1 (Novus Biologicals ...