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Citations for Bio-Rad :
1551 - 1600 of 8520 citations for 7 hydroxy 1H 1 2 4 triazolo 1 5 a pyrimidin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... The iTaq Universal SYBR Green One-Step Kit (Bio-Rad) was used to run the RT-qPCR reactions ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... and quantified by The Quantity One software v4.6.3 (Bio-Rad). Optical density values for target proteins were normalized to Ponceau staining of the nitrocellulose membrane as loading control ...
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bioRxiv - Immunology 2021Quote: ... using the iTaq Universal Probes One-Step RTqPCR kit (BioRad) with N2 primers and probes targeting the nucleocapsid (36) ...
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bioRxiv - Molecular Biology 2021Quote: ... The signals were quantified using Quantity One software (Bio-Rad), and normalized to the last sample (set as 1) ...
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bioRxiv - Immunology 2021Quote: ... using the iTaq Universal Probes One-Step RTqPCR kit (BioRad) with N2 primers and probes targeting the nucleocapsid 41 ...
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bioRxiv - Bioengineering 2022Quote: iTaq Universal Probes One-Step Kit (Biorad, cat. no. 1725141)
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bioRxiv - Developmental Biology 2022Quote: ... Expression was evaluated by Quantity One® (v4.6.7, Bio-Rad) densitometry to adjust for equal amounts of expression using WCL of untransfected cells to maintain total protein content ...
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bioRxiv - Immunology 2022Quote: ... the iTaq Universal Sybr green One-step kit (Bio-Rad) was used ...
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bioRxiv - Molecular Biology 2022Quote: ... or a QX ONE Droplet Digital PCR System (Bio-Rad) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... The gel was activated using one-minute setting (Bio-Rad Imager ...
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bioRxiv - Neuroscience 2023Quote: ... controlled by The Quantity One software v 4.6.9 (Bio-Rad). For quantitative purposes ...
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bioRxiv - Cell Biology 2023Quote: ... Band intensity was measured using Quantity One software (Bio-Rad). Quantitative ratios were calculated based on the data and are presented as relative values.
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bioRxiv - Genetics 2021Quote: ... membranes were incubated 2h in TBS-T with 2% BSA containing secondary anti-rabbit antibodies-HRP conjugate (1:2000, #1721019 Bio-Rad, Hercules, CA). After three more washes in TBS-T ...
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bioRxiv - Immunology 2022Quote: ... then blocked for 1 to 3 hr at RT with 50 μL/well of blocking buffer: 2% Blotting Grade Blocker (Bio-Rad cat. # 1706404) and 2% heat-inactivated goat serum (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... The results were obtained from channels 1 (FAM) and 2 (HEX/VIC) and analysed with QuantaSoft™ Analysis Pro 1.0 (Bio-Rad Laboratories, Inc). All ddPCR reactions were prepared with QX200™ ddPCR™ EvaGreen® Supermix (2x).
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bioRxiv - Microbiology 2020Quote: ... Real time quantitative PCR (qRT-PCR) was performed using an iQ™5 Multicolor Real-Time PCR Detection System (BIO-RAD) with qPCRBIO SyGreen Mix fluorescein (PCR BIOSYSTEMS) ...
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bioRxiv - Cancer Biology 2021Quote: ... diluted in nuclease-free water and the primer(s) were incubated at 65°C for 5 minutes in the DNA Engine Dyad®Peltier Thermal Cycler (BioRad). Afterward ...
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bioRxiv - Cell Biology 2020Quote: ... to pH 8.88 first for 5 min at 95 °C followed by 60 °C for 3 h on a T100 Thermal Cycler (Bio-Rad) with the heated lid set to 105 °C ...
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bioRxiv - Cell Biology 2020Quote: ... The blots were washed four times with TBST for 5 minutes each before developing with Clarity Western ECL Blotting Substrates (Bio-Rad) and imaging ...
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bioRxiv - Genetics 2019Quote: ... and heated at 90°C for 5 min before being run on a stain free gel and imaged by GelDoc imager (BIO-RAD).
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bioRxiv - Evolutionary Biology 2021Quote: ... Membranes were washed three times with TBST for 5 minutes and incubated with Goat Anti-Rabbit IgG (Bio-Rad, 170-6515) for 1 hour at RT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... to 10 μL RNA and incubating at 65°C for 5 min on a C1000 Touch Thermal Cycler (Bio-Rad, USA) followed by chilling on ice ...
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bioRxiv - Developmental Biology 2020Quote: ... 50 seconds at 72°C and a final extension of 5 minutes at 72 °C in a thermal cycler (T100TM Thermal Cycler, Bio-Rad). In order to standardize the expression pattern of NvDsx ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant was mixed with 5× RT Reaction Buffer and 10× RT Enzyme Mix and transferred to a thermal cycler (Bio-Rad). Cycling conditions were as follows ...
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bioRxiv - Cell Biology 2022Quote: ... Up to 5 μg of total RNA was reverse transcribed into cDNA using iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad). Quantitative real time-PCR was performed using GoTaq® qPCR Master Mix (Promega ...
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bioRxiv - Physiology 2019Quote: For serum fetuin-A analysis murine serum was separated by sodium dodecyl sulphate polyacrylamide gel elecrophoresis using mini gels (10% acrylamide, 5 × 8 × 0.1 cm3, BioRad, Hercules, USA). Protein transfer onto nitrocellulose membrane was by semi-dry electroblotting (Owl HEP-1 ...
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bioRxiv - Developmental Biology 2019Quote: ... Reverse primer: 5’-GCAGAAGAAGCAGACACAGC-3’) were PCR amplified and monitored using a CFX96 Touch Real-Time PCR detection system (Bio-Rad). Relative expression of PHETA1 transcripts was normalized to the expression of POLR2A and analyzed using standard delta delta Ct method ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed 3 times for 5 min with TBST after secondary antibody incubation and chemiluminescence signal was detected using Clarity Western ECL Substrate (Bio-Rad). All relevant information on antibodies used in this study are listed in Table S2.
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bioRxiv - Bioengineering 2021Quote: ... This gel was run for 5 min at 150 V in 1x MOPS buffer and stained using Coomassie blue (Bio-Rad) for 1 h with rocking ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.5 to a salt concentration of 100 mM NaCl and loaded onto a 5 mL Bio-Scale TM Mini CHT Type II column (Bio-Rad) equilibrated with CHT column buffer (5% glycerol (w/v) ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Protein was eluted from beads by heating for 5 min at 95 °C in 1X Laemmli Buffer (Bio-Rad, Hercules, CA). Retrieved protein was detected by standard immunoblotting techniques using the following antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... The reaction components were prepared as a 2x-master mix and 5 µl of the mix was pipetted into opaque wells of 384-well PCR-plate (Bio-Rad). Then an equal volume of sample was added ...
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bioRxiv - Cell Biology 2021Quote: ... samples were boiled for 5 min and proteins were resolved on 10% SDS polyacrylamide gels and blotted onto nitrocellulose membranes (Bio-Rad). Anti-rabbit Phospho-p44/42 MAPK antibody (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: ... Assays comprising of premixtures of a forward and reverse primer (18 μM each) with a FAM- or HEX-conjugated hydrolysis probe (5 μM) were either purchased from Bio-Rad, based on previous publications (Roberts et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Gene expression analysis were performed by quantitative RT-qPCR in a Viia7PCR device with 5 μl reaction mix containing SyberGreen Supermix (Bio-Rad). Tomato actin was used as a reference gene and fold change in expression were calculated based on 2^(-ddCt ...
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bioRxiv - Bioengineering 2021Quote: ... 15 µg of the extracted protein samples were boiled for 5 minutes at 95 °C in 4X laemlli buffer (BioRad; 1610747), run on mini-PROTEAN TGX precast gels (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... The total PCR reaction volume was 15 µL per well (10 µL master mix + 5 µL cDNA sample) and the qPCR was performed on the CFX384 Touch Real-Time PCR Detection System (Bio-Rad) using the cycling conditions listed below:
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bioRxiv - Microbiology 2020Quote: ... followed by ExtrAvidin alkaline phosphatase conjugate and alkaline phosphatase substrate: p-nitro blue tetrazolium chloride enhanced 5-bromo-4chloro-3-indolyl phosphate (Bio-Rad). The membranes with the transferred rgp120 were incubated with the HRP-conjugated V5 antibody (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed again for 3 × 5-min in TBS-T before being immersed for 5 min under gentle agitation at room temperature in Clarity ECL detection substrate (Bio-rad Laboratories Pty Ltd ...
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bioRxiv - Cell Biology 2022Quote: ... Standard qPCR reactions with 5 or 10 ng cDNA per reaction were performed with iTaq Universal SYBR Green Supermix (Bio-Rad) in the CFX Connect Real-Time PCR Detection System ...
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bioRxiv - Molecular Biology 2022Quote: ... Gels were run at 90 V for 120 min and there-after stained with Ethidium bromide at room temperature for 5 min and visualised using a Gel Doc XR imaging system (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: ... for 30 minutes at room temperature was followed by three 5 minute washes in milliQ water before imaging on a ChemiDoc MP (Bio-Rad).
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bioRxiv - Biophysics 2022Quote: ... TIA1 LC domain was isolated from the supernatant using a Bio-Rad NGC Discover 10 chromatography system and a 5 mL Ni-affinity column (Bio-Rad Bio-scale Mini Nuvia IMAC or GE Healthcare HisTrap FF crude) ...
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bioRxiv - Cell Biology 2022Quote: ... denatured in 2x Laemmli buffer (5 min at +95°C) and 20 µg were loaded per well of Mini-PROTEAN Precast Gel (Bio-Rad). The protein transfer was done with the Trans-Blot Turbo system (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... The stoichiometry of condensin II subunits for each preparation was checked by 5% SDS-PAGE followed by Coomassie Brilliant Blue R-250 (Bio-Rad) staining ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with beta-mercaptoethanol and denaturated at 95 °C for 5 min before loading on a 12 % PAA precast gel (#4561044, Bio-Rad). The gel was run at 70 V for 15 min and then 100 V for 1 h ...
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bioRxiv - Microbiology 2020Quote: Total protein extracts (5 × 106 cells) were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVFD Membranes (Bio-Rad) [55] ...
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bioRxiv - Developmental Biology 2020Quote: ... Total protein lysates were prepared from 25 pair of testes and loaded in equal dilution series (5, 10, and 20 μg) onto precast stain-free PAAG gel (Bio-Rad). After electrophoresis ...
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bioRxiv - Immunology 2020Quote: ... Protein for immunoblotting was eluted from 25% of beads by heating at 95°C for 5 minutes in 2X Laemmli sample buffer (Bio-Rad). RNA was extracted from the remaining 75% of beads using TRIzol (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... The lysates were boiled for 5 min at 95°C in 1x XT sample buffer containing 1x XT reducing agent (both from Bio-Rad) and resolved on 4-12% Bis-Tris XT precast gels (Bio-Rad) ...