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Citations for Bio-Rad :
1801 - 1850 of 3415 citations for C Type Lectin Domain Family 2 Member B CLEC2B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Samples were washed 3 times with ice-cold PBS and boiled at 90 °C in 2x Laemnli sample buffer (Bio-Rad 1610737) for 5 minutes the following day for elution.
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bioRxiv - Molecular Biology 2023Quote: ... the sample boiled for five minutes at 95 °C and size-separated by SDS-PAGE using a 4–20% Mini-PROTEAN® TGX™ Gel (Biorad). Proteins were transferred onto a nitrocellulose membrane (Biorad ...
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bioRxiv - Biochemistry 2022Quote: ... adjusted to equal concentration for each sample and boiled at 95°C for 10 minutes in Laemmli buffer (Bio-Rad #161-0747).
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bioRxiv - Bioengineering 2023Quote: ... before injecting into 1 mL Hitrap Heparin HP column pre-equilibrated with Wash Buffer C operated in the BioLogic DuoFlow™ FPLC system (Bio-rad). The protein was eluted from the column by running a gradient flow rate that exchanges Wash Buffer C and Elution Buffer D (2000 mM NaCl ...
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bioRxiv - Immunology 2022Quote: ... alum pellets were resuspended in sample loading buffer and boiled at 95 °C for ten minutes before being applied to a precast polyacrylamide gel (4–20% Mini-PROTEAN®, Bio-Rad). Proteins were then transferred to a nitrocellulose membrane using the Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... heated up for 10 minutes at 95°C and centrifuged before loading onto a 10 well 5-20% gradient gel (BioRad 456-1093). The finished gels were blotted via semi dry method (20V ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were denatured at 95°C for 10 mins and subjected to SDS-PAGE in a 4–12% Criterion™ XT Bis-Tris Protein Gel (Bio-Rad) using XT MES Running Buffer (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... The samples were then separated by 6.5% (w/v) native polyacrylamide gels at 4 °C and the biotin-labeled probes was captured with ChemiDoc™XRS+ (Bio-Rad). The oligonucleotide probes used in this study are listed in Supplemental Data Set S1.
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bioRxiv - Neuroscience 2023Quote: ... The cell suspension was collected and centrifuged at 4°C for 15 minutes at 10,000g and cell protein extracts were stored at −80°C or brought forward for total protein quantification using Bradford assay (Bio-Rad; USA). Cell protein samples were suspended in 2x Laemmli buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were boiled at 95°C for 5 min and loaded into 4-12% Criterion XT-Bis-Tris polyacrylamide gels (Bio-Rad 3450125). Gel electrophoresis was performed in 1X MES running buffer (Bio-Rad 1610789 ...
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bioRxiv - Biophysics 2023Quote: ... This mixture was then incubated at 4 °C for 1 hour and further subjected to a 4-hour incubation with bio-beads (Bio-Rad, USA). After incubation ...
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bioRxiv - Biochemistry 2023Quote: ... followed by heat-inactivation at 75°C for 5 min and desalting using Micro Bio-Spin P-6 columns (Bio-Rad, 7326221). Dephosphorylated guide RNAs were then radiolabeled using T4 Polynucleotide Kinase (0.33 U/µL ...
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bioRxiv - Cancer Biology 2023Quote: Pellets of 1×106 treated cells were incorporated in 1% low melting agarose at 50°C and cast into molds (Biorad, Grabels, France) avoiding air bubbles ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were eluted from sepharose beads by boiling at 95 - 100 °C for 5 min with 40 µl sample buffer diluted from 4x sample buffer (Bio-Rad, 1610747) with Lysis Buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at full speed for 10 minutes at 4⁰C and the total protein concentration in the supernatant was quantified using the detergent compatible (DC) protein assay (BioRad, 500-0114). Equal amounts of protein were mixed with anionic detergent (4x LDS loading buffer ...
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bioRxiv - Biochemistry 2023Quote: ... applying a constant current of 300 mA at 4°C for 1 hour using a Mini Trans-Blot® Cell (Bio-Rad). For the immunodetection of specific protein targets ...
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bioRxiv - Genetics 2023Quote: ... and 0.2 μl of 10X NEB 2.1 buffer were mixed gently in a PCR tube and incubated at 25°C for 15 minutes in a Thermal Cycler (Bio-Rad T100) and then 1 μL ssDNA repair template [IDT] and Phenol red (0.3 μL ...
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bioRxiv - Biochemistry 2024Quote: ... were annealed at a ratio of 3:1 molar ratio Incubated at 93°C for 3 minutes and slowly cooling down in C1000 Touch™ Thermal Cycler (Bio-Rad). The pre-annealed RNA-TS (32 µM ...
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bioRxiv - Molecular Biology 2023Quote: ... The denatured lysate was then clarified by centrifugation (13200 rpm, 10 min at 4°C) and protein concentration was determined using the DC™ Protein Assay (Bio-Rad). Either equal amounts or a serial dilution of the lysates from various strains were resolved using SDS-PAGE and transferred onto a polyvinylidene difluoride membrane ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein samples were boiled for 10 minutes at 95 °C and loaded onto Mini-PROTEAN TGX™ Precast Gels (BIO-RAD:4569036), and electrophoresis was performed according to manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... destained for 2 h at room temp (Coomassie Brilliant Blue R-250 staining kit, BIO-RAD), and imaged on a ChemiDoc MP (BIO-RAD).
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bioRxiv - Molecular Biology 2020Quote: ... The mixture was then treated with 5% volume of Bio-Bead SM-2 resin (Bio-Rad) with shaking on ice for 15 min ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 specific genome levels were measured by qPCR using SsoFast EvaGreen supermix (Bio-Rad) according to manufacturer’s protocol.
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were then transferred (200 mA for 2 hours) to polyvinylidene difluoride membranes (Bio-Rad Laboratories), Ponceau stained ...
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bioRxiv - Biochemistry 2020Quote: ... Biotinylated small RNAs were separated from HPDP-biotin and pyridine-2-thione using spin columns (BioRad) in ultra-pure water.
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bioRxiv - Developmental Biology 2020Quote: ... We ran the qPCR reactions using 1-2 µl cDNA in SYBR Green Master Mix (Biorad) totaling 20 µl in a Biorad CFX96 machine using 60°C as the annealing temperature ...
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bioRxiv - Bioengineering 2021Quote: ... and the selected primers (Table 2) using a CFX Connect™ Real-Time System (Bio-Rad) under the following conditions ...
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bioRxiv - Cell Biology 2021Quote: Samples for electrophoresis were prepared by mixing with 2× Laemmli sample buffer (Bio-rad, Hercules, California) and denatured at 55°C for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... 2-3 μg of total protein were loaded onto 10% SDS-polyacrylamide (29:1 Bio-Rad) gels with 10 μM phostag reagent (FUJIFILM Wako Chemicals ...
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bioRxiv - Microbiology 2020Quote: ... in a 100 μL reaction for 2 hours and purified using P-30 spin columns (BioRad). The radiolabeled probe was heated to 100°C for 2 minutes and resuspended in 10 mL of ULTRAhyb Oligo hybridization buffer (Ambion) ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesised from 2 µg purified RNA using iScript™ cDNA Synthesis Kit (Bio-Rad) and quantified by qRT-PCR using iTaq™ Universal SYBR® Green Supermix ...
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bioRxiv - Cancer Biology 2020Quote: ... Two percent agarose gel was prepared by dissolving 2 g Agarose (Bio-rad, Cat No. 1613102) and 2 µL SYBR Safe DNA Gel Stain (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... The beads were washed 3 times and then eluted using 2 × Laemmli Sample Buffer (Bio-Rad). The elution was subjected to SDS PAGE to run the sample into the lane ...
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bioRxiv - Developmental Biology 2022Quote: ... Supernatant fractions were collected by centrifugation and boiled with 2× Laemmli sample buffer (Bio-Rad, 1610737) at 95 °C for 8-10 min ...
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bioRxiv - Biochemistry 2022Quote: Cell lysates were diluted to 2 µg/µl in Laemmli buffer (Bio-Rad, Hercules CA, 1610747) and heated at 95ºC for 5 minutes and resolved using a 4-20% tris-glycine gel (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were mounted on slides in reagent 2 in frame-seal incubation chambers (Bio-Rad SLF0601) and coverslipped ...
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bioRxiv - Biophysics 2020Quote: ... Detergent was slowly removed by the addition of SM-2 Bio-Beads (Bio-Rad, Hercules, CA) over a 4-hour time course (final weight ratio of 25 Bio-Beads to 1 detergent) ...
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bioRxiv - Biophysics 2020Quote: ... Nanodisc reconstitution was achieved by incubation with 0.5 - 1 mL Bio-Beads SM-2 (Bio-Rad) for 16 hours at 4°C under constant rotation ...
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bioRxiv - Microbiology 2021Quote: ... each cDNA was combined with segment-specific primers (Table 2) and Precision Melt Supermix (Bio-Rad) and analyzed by qPCR using a CFX384 Touch real-time PCR detection system (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: ... mixed thoroughly and the mixture transferred to an electroporation 2 mm-gap width cuvette (Bio-Rad). Electrotransformation was performed in a Micropulser™ device (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: RNA samples (2 μg) were loaded onto a 10% Criterion TBE-urea precast gels (Bio-Rad) and electrophoresed at 70 V until the dye front reached the bottom of the gel ...
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bioRxiv - Cancer Biology 2019Quote: ... 5% of the lysates were boiled with 2 x laemmli buffer (BIO-RAD, Hercules, CA, USA) and used as input ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed in monomer solution (1 x PBS, 2 M NaCl, 2.5% acrylamide (Bio-Rad), 0.15% Methylenbisacrylamide (Santa Cruz) ...
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bioRxiv - Biophysics 2021Quote: ... The detergent was then removed upon incubation with Bio-Beads SM-2 sorbent (Bio-Rad Laboratories) overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Cleaned amplified products were again run (5μl loaded) on 2% Agarose gel (Bio-Rad, California, USA) to confirm no further contamination ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were mounted on slides in reagent 2 in frame-seal incubation chambers (Bio-Rad SLF0601) and coverslipped (#2 glass) ...
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bioRxiv - Biochemistry 2021Quote: ... The bound neutravidin beads were washed in 2 ml Bio-spin column (Bio-Rad, 732-6008) with 5 ml RIPA buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The detergent was removed using a Bio-Beads SM-2 resin (Bio-Rad, Hercules, CA, USA). The protein solution was concentrated to 10 – 20 mg/mL using a Centrisart I MWCO 100 kDa concentrator (Sartorius AG).
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bioRxiv - Biochemistry 2021Quote: ... The cells were transferred into ice-cold 2 mm electroporation cuvettes (Bio-Rad, Hercules, United States) and electroporated (2500 V ...
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bioRxiv - Genomics 2021Quote: ... Each empty/filled reaction was performed using a DNA Engine Tetrad 2 Thermal Cycler (Bio-Rad) and Expand Long Range Enzyme Mix ...