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Citations for Bio-Rad :
1551 - 1600 of 2175 citations for Nonanoic acid reaction products with diethanolamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was prepared from 500ng RNA per reaction using the iScript cDNA Synthesis Kit (#1708891, Bio-Rad, Hercules, CA). QPCR was performed using probe-based primer sets and master mix (Prime Time ...
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bioRxiv - Cell Biology 2022Quote: ... Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) was performed using SsoAdvanced Universal SYBR® Green Supermix (1725274; BioRad) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... 22-μL reactions were made using Bio-Rad’s ddPCR Supermix for Probes (No DUTP) (Bio-Rad Laboratories, Richmond, CA). Each ddPCR reaction contained 16.5 μL of an assay mix with 11 μL of Supermix ...
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bioRxiv - Cancer Biology 2022Quote: ... The reaction mixture was separated on an 8% native polyacrylamide gel and visualized using a ChemiDoc Imaging system (BioRad) with SYBR Green detection filter.
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bioRxiv - Bioengineering 2022Quote: ... qPCRs were conducted in 20 μL of reaction mixtures comprising: 10 μL of IQTM SYBR Green Supermix (Bio-Rad), 2 μL of DNA sample ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was generated from total RNA via reverse transcription reaction using an iScript RNA-to-cDNA kit according to the manufacturer’s instructions (BioRad). cDNA was then amplified using the iQ SYBR Green Supermix (Biorad) ...
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bioRxiv - Immunology 2022Quote: ... Quantitative real-time PCR reactions were performed on a Bio-Rad CFX96 thermal cycler (Bio-Rad Hungary, Budapest, Hungary) using the SensiFast SYBR Green PCR Master Mix (Thermo Fisher) ...
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bioRxiv - Genetics 2022Quote: ... Worm lysates or E3 ligase reaction mixtures were resolved on 4-20% stain-free SDS-PAGE gels (Bio-RAD) and transferred to Millipore Immobilon-P PVDF membranes ...
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bioRxiv - Plant Biology 2022Quote: ... Each RT-qPCR reaction (10 μl) included 2 × iTaq™ Universal SYBR®Green Supermix (Bio-Rad, CA, USA), 500 nM forward and reverse primers (Supplementary File 1C) ...
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bioRxiv - Microbiology 2023Quote: ... was used for qPCR assay in 20 μL of reaction mixture on a real-time PCR system (Bio-Rad) with the primer pairs listed in Table S2 ...
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bioRxiv - Zoology 2022Quote: Quantitative polymerase chain reaction (qPCR) was conducted in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, USA), using 96-well PCR plates ...
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bioRxiv - Microbiology 2022Quote: ... qPCR was performed by amplifying cDNA in 20 μL reaction volumes with iTaq Universal SYBR Green Supermix (Bio-Rad) in the CFX96 Touch Real-Time PCR System (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: Retrotranscription of RNA was performed using 3μg of total RNA in a 40μl final volume reaction mixture using iScript Advanced cDNA Synthesis Kit for RT-qPCR (Biorad) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cDNA was amplified in a 10 μL reaction mixture containing 5 μL of iTaq SYBR Green Supermix (Bio-Rad) and 0.5 μM each of gene-specific forward and reverse primer ...
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bioRxiv - Plant Biology 2023Quote: ... qPCR reactions were performed and quantitated in a Bio-Rad CFX96 system using SsoAdvanced Universal SYBR Green Supermix (BioRad). The primers (0.3 µM ...
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bioRxiv - Biochemistry 2023Quote: ... The antibody–antigen reactions were detected using the Clarity™ or Clarity™ MaxWestern Blotting ECL Substrates (Bio-Rad). Densitometry analysis was performed using ImageJ software11–13 ...
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bioRxiv - Cell Biology 2023Quote: ... All reactions were performed in 3 technical replicates using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Data was analysed according to the 2-ΔΔCT method52.
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bioRxiv - Cell Biology 2024Quote: ... PCR amplification was performed in duplicate for each reaction on a CFX384 Touch Real-Time PCR (Bio-Rad, USA). The cycling conditions were 95°C (3 min) ...
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bioRxiv - Microbiology 2024Quote: ... Amplification was similarly performed in 20 µL reaction volumes containing 10 µL of SYBR SsoAdvanced SYBR Green Supermix (BioRad), 4µL of sterile milliQ water ...
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bioRxiv - Microbiology 2024Quote: ... qPCR was performed by amplifying cDNA in 20 µL reaction volumes with iTaq Universal SYBR Green Supermix (Bio-Rad) in the CFX96 Touch Real-Time PCR System (Bio393 Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... https://di-ning.com.cn/) was used for cDNA analysis on the iQ5 multicolor real-time Polymerase Chain Reaction Detection System (Bio-Rad). Data were analyzed using iQ5 2.0 standard optical system analysis software and the 2−ΔΔCT method (Livak and Schmittgen 2001).
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bioRxiv - Microbiology 2024Quote: ... Singleplex ftsZ and mid1 reactions consisted of 10 µL of ddPCR Supermix for Probes (no dUTP) (Bio-Rad, 1863023), 0.5 µL of the relevant oligo set ...
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bioRxiv - Molecular Biology 2024Quote: qPCR reactions were performed on a CFX384 thermal lightcycler in 384-well plates (white hard-shell thin wall, BioRad) using PrecisionPLUS SYBR green mastermix (Primerdesign) ...
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bioRxiv - Molecular Biology 2024Quote: ... whereafter 5 μl was used for a 25 μl qPCR reaction using 2x SYBR Green Master Mix (Bio-Rad) in a CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: A 22ul reaction mixture was prepared comprising 11ul 2×ddPCR Supermix for probes (no dUTP) (Bio-Rad, CA, USA), 0.55 ul of TRECs primers (36uM stock ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR reactions were conducted in 96-well plates using a CFX96 Real- Time System (BIO-RAD, Berkeley CA). A 20 μL reaction mix ...
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A comparative analysis between two flax varieties indicates lignan-mediated salt stress adaptivenessbioRxiv - Plant Biology 2024Quote: ... qPCR was performed using the C1000 thermal cycler with CFX96 Real-Time System optical reaction module (Bio-Rad, USA). Expression of Cyclophilin and ETIF5A was used to normalise the miRNA expression levels ...
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bioRxiv - Synthetic Biology 2024Quote: ... Reactions were prepared on ice in a PCR hood before being transferred to a preheated thermocycler (Bio-Rad, C1000). Nuclease-free water (Omega Biotek ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative PCR (qPCR) reactions were carried out in triplicate on specific genomic regions using SYBR Green Supermix (Bio-Rad). The resulting signals were normalized for primer efficiency by carrying out qPCR for each primer pair through Input DNA ...
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bioRxiv - Biochemistry 2020Quote: ... and the quantity of radiolabel included into the acid insoluble fraction was analysed using Quantity One software (Bio-Rad). The quantitative data were analysed using MS Excel 2010 and presented on histograms as the Mean ± SD.
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bioRxiv - Biochemistry 2021Quote: ... Approximately 20 ug of acid-extracted protein was run in triplicate on a 4-20% SDS-PAGE gel (BioRad) and a single band corresponding to P1 and P2 was cut out for processing ...
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bioRxiv - Immunology 2023Quote: ... in non-reducing conditions and run at 120 V in 3-Morpholinopropane-1-sulfonic acid (MOPS) buffer (Bio-rad). Bands were visualized with Imperial Protein Stain (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... in non-reducing conditions and run at 120 V in 3-Morpholinopropane-1-sulfonic acid (MOPS) buffer (Bio-Rad). Bands were visualized with Imperial Protein Stain (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad, France). The results were analyzed using Bio-Rad CFX Maestro software ...
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bioRxiv - Biochemistry 2022Quote: ... and anti-capsid/p24 antibodies were developed following incubation with horseradish peroxidase-conjugated goat anti-rabbit IgG antibody (BioRad; product code 1706515) or IRDye 800CW conjugated goat anti-rabbit IgG antibody (LI-COR ...
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bioRxiv - Microbiology 2022Quote: ... the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad, France). The results were analyzed using the Bio-Rad CFX Manager software ...
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bioRxiv - Genomics 2020Quote: ... The amplified products were electrophoresed on an agarose gel and visualized with the Gel Doc EZ imager (Bio-Rad, Hercules, CA, USA). Then ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified and normalized PCR products were further pooled into a single library and quantified using qPCR (KAPA Library Quant Kit qPCR Mix, Bio-Rad iCycler). The final library was diluted to 4 nM and sequenced in an Illumina HiSeq 2500 Platform using a 2×250bp RapidRun kit for an average coverage of 160,000 paired reads per PCR product.
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bioRxiv - Microbiology 2022Quote: ... The amplification kinetics of each product were checked at the end of each cycle by measuring the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad, France). The results were analyzed using Bio-Rad CFX Maestro software ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR was performed using 1:10 dilutions of cDNA synthesis products with the iTaq Universal SYBR green supermix (Bio-Rad; 1725121) on a CFX Connect real-time PCR detection system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the appearance of intermediates (jm) and products (nc) were quantified from the gels using a Geldoc system and the Image Lab software (BioRad, Hercules, USA), as described [30] ...
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bioRxiv - Microbiology 2022Quote: ... the fluorescence of the EvaGreen dye incorporated into the PCR product was measured at the end of each cycle using SoFast EvaGreen Supermix 2× kit (Bio-Rad, France). A standard curve using gDNA of purified viruses was performed in parallel of each experiment ...
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bioRxiv - Microbiology 2024Quote: ... the fluorescence of the EvaGreen dye incorporated into the PCR product was measured at the end of each cycle using SoFast EvaGreen Supermix 2× kit (Bio-Rad, France). A standard curve using the gDNA of purified viruses was performed in parallel with each experiment ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were run on a 1% Agarose gel containing .08% ethidium bromide and visualized using the ChemiDoc imaging system (BioRad, Hercules, USA). PCR amplicons were sanger sequenced (Psomagen ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was analysed using agarose gel electrophoresis system and visualized by gel documentation (Gel Doc, Bio-Rad, Hercules, California, USA). The 650 bp positive fragment was purified by Wizard® SV Gel and PCR Clean-Up System (Promega Corporation ...
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bioRxiv - Microbiology 2023Quote: ... before being incubated for 1 h in water with 3X GelRed (Phenix Research Products) and imaged on ChemiDoc XRS imaging system (Bio-Rad Laboratories) and Quantity One imaging software (Bio-Rad Laboratories).
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bioRxiv - Cancer Biology 2024Quote: ... After SDS SDS-PAGE and transferring to a PVDF membrane using the Transblot® SD Semi-Dry Electrophoretic Transfer Cell (Product #1703940, Bio-Rad), PVDF membranes were blocked in 5% skim milk for 15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... 3 µg of total RNA was used in the reverse transcription reaction using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR amplification was performed on the Prism 7900 Sequence Detection System (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... and equal amounts (1–2µg) were used for reverse transcriptase reaction using an iScript™ Reverse Transcription Supermix (Bio-Rad). qPCR was run using the SYBR® Green Master Mix on a CFX Connect™ Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... 500ng of RNA was reversed transcribed into cDNA in a 20 μL reaction volume using iScript cDNA synthesis kit (Biorad) in accordance to manufacturer’s guidelines ...