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Citations for Bio-Rad :
1451 - 1500 of 2175 citations for Nonanoic acid reaction products with diethanolamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Substrate and product bands were individually quantified as a percentage of the total signal for each time point using ImageLab (Bio-Rad). Ratios of ubiquitinated products relative to the total signal were plotted against the concentration of Ubc2 and fit to the Michaelis–Menten equation to estimate Km in GraphPad Prism 8 ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting products were analyzed on a 3% agarose gel stained with ethidium bromide and visualized using ChemiDoc XRS+ (Bio-Rad). The proportion of each splice isoform was quantified using the ImageJ software.
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bioRxiv - Plant Biology 2021Quote: ... Electrophoresis of PCR products was done on EtBr-stained 1% agarose gels and photographed on a Gel Doc XR instrument (Bio-Rad).
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bioRxiv - Biochemistry 2021Quote: ... Glycosylated products were separated from unincorporated UDP-[14C]-GalNAc by anion exchange chromatography using AG1-X8 resin columns (Bio-Rad #1401454), and product incorporation was determined by liquid scintillation counting (Beckman Coulter LS6500) ...
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bioRxiv - Plant Biology 2021Quote: ... A 25-fold dilution series of original reverse-transcription products was used for qRT-PCR using SsoAdvanedTM Universal SYBR Green Supermix (BioRad, USA) on CFX96TM Real-Time System (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... or immunoprecipitation products were denatured by boiling for 10 minutes and run on a 4-12% Gradient Bis-Tris Gel (Bio-Rad) before being transferred to a PVDF membrane (Millipore) ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR product was separated in a 2% agarose gel and imaged by ChemiDoc™ Touch Imaging System (Bio-rad Laboratories).
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bioRxiv - Neuroscience 2020Quote: ... Quantitative PCR for Glo1 was performed with iQ SYBR Green Supermix and amplified PCR products were measured by the iCycler iQ real-time PCR analyzing system (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... 14 µL of PCR product were then transformed by electroporation in 90 µL cells (2.5 kV electric pulse for 5 ms, Bio-Rad micropulser). Cells were recovered in SOC outgrowth medium (NEB B9020S ...
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bioRxiv - Synthetic Biology 2020Quote: ... Digested plasmids and PCR products were separated by DNA electrophoresis with 0.8 % (w/v) agarose gels and visualized using Molecular Imager VersaDoc (Bio-Rad, USA). The flawlessness of PCR-amplified genes cloned into target plasmids was checked by DNA sequencing (Macrogen ...
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bioRxiv - Microbiology 2020Quote: The quality of genomic DNA and the PCR products were checked on 1% (w/v) agarose gel made by dissolving 1.5 g of agarose (Bio-Rad, SA) in 150 ml of 1X Tris-acetate-ethylenediaminetetraacetate (TAE ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were identified via 1% agarose gel electrophoresis and visualized in a Gel Doc system (BioRad, Hercules, CA, USA).
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bioRxiv - Plant Biology 2021Quote: ... The reverse transcription products were used as templates for quantitative real-time PCR performed on a CFX96 real-time PCR system (Bio-Rad) using SYBR Green Master Mix (DBI Bioscience ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed with cold water and 10% glycerol and electroporated with the cat or kan PCR product using a GenePulser II instrument (2.5 kV, 0.2 cm gap cuvettes; Bio-Rad, Hercules, CA). Transformants were verified by colony PCR with primers approximately 200 bp up- and downstream of the gene of interest ...
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bioRxiv - Immunology 2020Quote: ... Purified products were analyzed by 10% TBE PAGE and total protein quantified with Bio-Rad protein quantification reagent (Bio-Rad, 5000006). LPS contamination after conjugation was below 0.5 EU/mg as mentioned below in the vaccinations section.
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bioRxiv - Microbiology 2020Quote: ... the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad). The results were analyzed using Bio-Rad CFX Manager software ...
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bioRxiv - Pathology 2023Quote: ... and DNA fragment (amplification products containing homologous DNA fragments) in a 4-mm Gene Pulser and electrotransformed by Gene Pulser Xcell system (Bio-Rad). Transgenic parasites were selected with pyrimidine (1μM ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR products were run on a 2% agarose gel and images were taken on a Chemidoc MP imaging system (BIO-RAD). Image J was used to analyze the pixel intensity of 3R (288 bp ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting products were analyzed on a 3% agarose gel stained with ethidium bromide and visualized using ChemiDoc XRS+ (Bio-Rad). The relative amounts of the resulting products were analyzed by scanning the gels and determining the intensities of ethidium bromide stained bands using Image Lab software Version 1.36b (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... The digestion products were analyzed by 12-15% SDS-PAGE and stained with Coomassie blue or silver (Silver stain plus kit, Bio-rad). Images were taken using a Chemidoc imaging system (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: ... Custom primers were designed to meet several criteria using NCBI Primer Blast followed by running of the complementary DNA (cDNA) synthesis product made using RT-PCR (iScript kit, Cat no. 1708890, Biorad, USA) on an electrophoresis gel ...
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bioRxiv - Microbiology 2023Quote: HPLC analysis of the SCFA fermentation products of 499 and 831b from glucose was performed using Aminex HPX-87H Column #1250140 (Bio-Rad). Sample tubes contained one mL of supernatant from a 24 h culture ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative analysis of PCR products was done by densitometry and carried out using ImageLab software (Image Lab Software, Life Science Research, Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... The PCR product was subjected to agarose gel electrophoresis and imaged on a ChemiDoc MP imaging system (BioRad, Hercules, CA, USA).
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bioRxiv - Immunology 2022Quote: ... The resulting GEM generation products were then transferred to semi-skirted 96-well plates and reverse transcribed on a C1000 Touch Thermal Cycler (Bio-Rad) programmed at 53°C for 45 minutes ...
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bioRxiv - Microbiology 2022Quote: ... ∼2 μg purified PCR products were electroporated into 50 μL electrocompetent cells at 1.75 kV using 0.1 cm cuvettes (Bio-Rad cat. 1652089) and a MicroPulser electroporator (Bio-Rad cat ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1 μg purified linear PCR products were added to the cell mixture and proceeded electro-transformation (Gene Pulser Xcell system (Bio-Rad) and Gene Pulser/Micropulser Electroporation Cuvettes ...
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bioRxiv - Genetics 2023Quote: ... Amplicon verification was performed by gel electrophoresis using 1.5% agarose gels and the PCR products were visualized using a Gel Doc XR+ Imaging System (Bio-Rad Inc.). The PCR products were then purified with the GeneJET PCR Purification Kit (Thermo fisher scientific ...
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bioRxiv - Neuroscience 2024Quote: ... and then melting curve analyses were performed for each reaction to confirm single amplified products between 65-95°C in 0.2°C increments on the Bio-Rad CFX96 system (Bio-Rad, USA). Relative quantification of mRNA of target genes across several time points was determined by taking the ratio of CT values divided by the CT values of glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Molecular Biology 2024Quote: ... was added to all samples and the products were separated on 15% polyacrylamide denaturing urea gels (19:1 acrylamide-bisacrylamide, Bio-Rad). The gels were fixed in fixing solution (40% methanol ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR Products were gel isolated from a 1% TAE agarose gel using Freeze ‘N Squeeze DNA gel extraction columns (Bio-Rad), following manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... PCR products were subsequently analyzed via agarose gel electrophoresis and the ratio of splice variants was assessed using ImageLab (Bio-Rad) or Fiji/ImageJ ...
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bioRxiv - Microbiology 2024Quote: ... Electroporation was carried out with 3-5 μL of the linear PCR product (metH homology extension-flanked kan cassette) at 2.5 kV (Bio-Rad MicroPulser), and cells were immediately recovered with 1 mL SOC and cultured at 37°C for one hour ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed again with TBST buffer (5X5 min) and developed using the Clarity Western ECL substrate (Bio-Rad, product #1705061). Images were captured on a luminescent image analyzer (ChemiDocTM MP imaging system ...
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bioRxiv - Biochemistry 2024Quote: ... and the product was directly used to transform competent Quick DH5α cells (TOYOBO) to obtain pGLO-high plasmid after miniprep (Bio-RAD). The GFPuv sequence in the pGLO-high plasmid was replaced with a megaprimer-based whole plasmid amplification (MEGAWHOP ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR reactions were performed in triplicates on the CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad). After an initial period of 3 min at 95 °C ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was diluted to 20 ng/µL in molecular grade water and 1 µL used for Sybr Green reactions in technical duplicate or triplicate as per manufacturer’s instructions on a BioRad CFX384 (BioRad). RT-PCR primers listed in Sup ...
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bioRxiv - Genetics 2021Quote: Each 25 μL ddPCR reaction contained 12.5 μL 2x Supermix for Probes (no dUTP) (Bio-Rad Laboratories, #186-3024), cDNA ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative PCR (QPCR) reactions were carried out in triplicate on specific genomic regions using SYBR Green Supermix (Bio-Rad). The resulting signals were normalized for primer efficiency by carrying out QPCR for each primer pair using Input DNA ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were performed in a clear 96-well plate using a CFX96 Real-Time PCR System (BioRad, Hercules, USA). Gene expression was assessed by normalizing to housekeeping gene tus using the R package qpcR (v1.4 ...
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bioRxiv - Neuroscience 2021Quote: ... The threshold amplification cycle number (Ct) was determined for each reaction by Quantitative RT-PCR (Bio-Rad CFX 96) in the linear phase of the amplification plot ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative real time-polymerase chain reaction (qRT-PCR) was performed on cDNAs using iQ SYBR green supermix (Bio-Rad) in a CFX-96 Touch system (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... The reaction mixture (total volume 22 μl) contained 11 μl of 2× ddPCR Super Mix for Probes (BioRad, 1863024), 1.1 μl of 4 primers mixture 18 μM each ...
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bioRxiv - Genomics 2020Quote: ... USA) and performed the reaction using a CFX384 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). We analyzed the Cq values using a standard curve method and compared the number of transcripts in each sample to the number of small subunit mitochondrial rRNA.
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bioRxiv - Genetics 2020Quote: ... 2 mM ATP and 1 mM DTT and the reactions were stopped by addition of Laemli sample buffer (BioRad). Samples were run on precast 4-20% gradient SDS-PAGE gels (BioRad ...
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bioRxiv - Immunology 2021Quote: ... Quantitative PCR was performed in 20 μL reactions in triplicate containing 10 μL 2 × SsoFast EvaGreen Supermix (Bio-Rad,), 1 μL of primers (3 μM F and 3 μM R) ...
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bioRxiv - Bioengineering 2021Quote: ... Amplifications were performed in a 20 μL reaction containing 1 × ddPCR Supermix for Probes (No dUTP),(Bio-Rad, 1863024), 250 nM of probes and 900 nM of primers and 8 μL EV DNA template ...
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bioRxiv - Neuroscience 2020Quote: ... SYBR green reactions were performed following the manufacturer’s protocols in a CFX96 Touch Real Time PCR Detection System (BioRad) using 4ng of total cDNA ...
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bioRxiv - Microbiology 2020Quote: ... A total of 20 µl of each reaction was electrophoresed on a SDS-PAGE (Bio-Rad; Cat#456-1095). The gels were stained using Coomassie blue.
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative PCR reactions were carried out using the iTaq™ Universal SYBR® Green Supermix (Bio-Rad, Hercules, CA) according to the manufacturer’s instructions in a CFX Connect Real-Time PCR Detection system (Bio-Rad ...