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Citations for Bio-Rad :
1301 - 1350 of 1976 citations for MBL 2 Mouse HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Gels were dried for 2 h at 80°C and analysed on a Personal Molecular Imager (Bio-Rad). For RNA stability assays ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of RNA were mixed with 2 μl of 50 μM oligo dT primer (Bio-Rad, 1725038) and 1 μl of 25 mM dNTP mix (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... anti-α-tubulin YL1/2 from rat (MCA77G, Bio-Rad AbD Serotec, at 1:2000 dilution for IF), anti-rabbit Alexa Fluor 488 (A11008 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 2 μl of the cDNA sample were supplemented with 5 μL of SsoADV Universal SYBR Green Supermix (BioRad) mix 2X ...
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bioRxiv - Genetics 2019Quote: ... the size of the amplicon was determined by comparison with a 2-kb DNA marker with the use of Quantity One software (version 4.6.7; Biorad), and the number of repetitions was estimated by comparing the size of the product amplified from the Nichols strain ...
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bioRxiv - Molecular Biology 2023Quote: ... Captured proteins were separated from beads by incubating beads in 50 μl 2× Laemmli Sample Buffer (Bio-Rad) at 95°C for 25 min ...
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bioRxiv - Immunology 2022Quote: ... 200 μL of this transformation mix was then aliquoted into pre-chilled 2 mm electroporation cuvettes (Bio-Rad) and electroporated at 1500 V with an average time constant of ∼4.5 ms using a Gene Pulser Xcell Electroporation System (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: ... Protein samples were suspended in Laemmli Sample Buffer supplemented with 2-Mercaptoethanol (β-mercaptoethanol) (BioRad Hercules, California – USA). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... qPCR was performed using the Promega 2-step kit on a CFX96 Real-Time PCR System (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... heated to 50°C for 2 min before addition of 40 µl molten CleanCut agarose (Bio-Rad 1703594), vortexing vigorously for 5 s before pipetting in plug mould (Bio-Rad 1703713 ...
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bioRxiv - Plant Biology 2023Quote: ... 2 ug RNA was used to synthesize cDNA with the iScript™ cDNA Synthesis Kit (Bio-Rad 1708890). The SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad 1725271 ...
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bioRxiv - Physiology 2023Quote: ... and samples of four larvae each were homogenized in 100 μl 2× SDS sample buffer (Bio-Rad #1610737) containing 5% (355 mM ...
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bioRxiv - Cell Biology 2023Quote: ... Three hundred micrograms of protein was precipitated using a ReadyPrep 2-D Cleanup Kit (Bio-Rad Laboratories, USA) at −20 °C overnight ...
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bioRxiv - Immunology 2023Quote: ... unreacted ITC-DTPA was removed by dialysis against 0.25 M ammonium acetate (NH4Ac) pH 5.4–5.5 (metal free) supplemented with 2 g/L Chelex (Bio-Rad) using 20,000 molecular weight cut-off dialysis cassettes (Slide-a-Lyzer ...
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bioRxiv - Biochemistry 2023Quote: ... The detergent was removed by three 2-hour incubations with ∼33 mg of Bio-Beads SM2 (Bio-Rad) followed by overnight incubation with ∼50 mg of Bio-Beads SM2 with all incubations occurring at 4°C.
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was synthesized from total RNA (1-2 μg) with iScript Advanced cDNA Synthesis Kit (Bio-Rad; 1725038). Quantitative RT-PCR (qPCR ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA synthesis was performed using 2 μg of total RNA and the iScript cRNA synthesis kit (Bio-Rad). qPCR was performed using the Brilliant III ultra-Fast SYBR green qPCR master mix (Agilent Technologies ...
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bioRxiv - Synthetic Biology 2024Quote: ... was added into (Ribosome purification: 5 mL; PURE proteins purification: 2-3 mL) Econo-Pac chromatography columns (Biorad). The column was washed with 50 mL deionized Millipore water and charged with 15 mL 0.1 N Nickel sulphate solution ...
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bioRxiv - Cell Biology 2023Quote: Protein samples were added with equal volume of 2× Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA, USA) containing 65.8 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was then cleaned 2-times on Micro Bio-Spin™ P-6 gel Columns (BioRad, #732-6221) where buffer was exchanged with phase separation buffer ...
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bioRxiv - Genomics 2023Quote: ... proteins were transferred (80 V; 2 h; 4°C) onto a 0.2 µm nitrocellulose membrane (Bio-Rad 1620112). Membranes were air-dried ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was passed twice on 2 x Mini CHT ™ type I column (Bio-Rad, United States). Elution was performed with a gradient of 0 to 200 mM NaPO42- in 10 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... The mixture was transferred to an ice-cold 2 mm gap electroporation cuvette (Bio-Rad Laboratories GmbH, Germany), and cells were electroporated with a single pulse at 1.8 kV ...
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bioRxiv - Genomics 2023Quote: ... Gene panel probe hybridization occurred overnight for 18 hours at 50°C (Bio-Rad DNA Engine Tetrad 2). Subsequent washes the next day removed unbound probes ...
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bioRxiv - Genetics 2023Quote: ... 1 ml 2xYNB with 2% glucose was added to the sorted cells in sheath fluid (PBS, BioRad #12012932) and they were transferred to a shaking incubator and grown at 30 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μg of total RNA was used for cDNA preparation using the iScript cDNA Synthesis Kit (Bio-Rad). A 1/10 dilution of the cDNA was then used for qRT-PCR analysis utilizing the SYBR Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... and incubated with an S-specific mouse monoclonal antibody 2B3E5 (provided by Dr. Thomas Moran at ISMMS) or an HN-specific mouse monoclonal antibody 8H2 (MCA2822, Biorad) diluted in PBST containing 1% bovine serum albumin (BSA) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins and peptides bearing C-terminal 6xHis tags were detected with mouse anti-6xHis antibody clone AD1.1.10 (BioRad; Cat # MCA1396GA) while detection of glycosylated CRM197-FtO-PS was with anti-F ...
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bioRxiv - Immunology 2022Quote: ... Primary antibody incubation was performed by incubating slides with diluted monoclonal mouse α-human pan-cytokeratin antibody (Bio-Rad MCA1907T) overnight at 4 °C followed by secondary antibody incubation with HRP-labeled α-mouse antibody for 30 minutes RT.
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bioRxiv - Neuroscience 2020Quote: ... Samples were prepared and analyzed using a Bio-Plex Pro Mouse Cytokine 23-Plex Assay kit (Bio-Rad, Hercules, CA). Assays were performed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... membrane was washed repeatedly with PBS-T and incubated 1 h RT with the secondary antibody in milk PBS-T (Goat anti-mouse IgG HRP conjugate (170-6516; BioRad); Goat anti-rabbit IgG HRP conjugate (170-6515 ...
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bioRxiv - Pathology 2019Quote: ... Bands corresponding to EPCR and the housekeeping protein β-actin (mouse IgG #NB600-501, Novus, at 1:40,000) were detected by the chemiluminescence method (Clarity Western ECL Bio-Rad) and finally densitometrically measured by ImageJ 1.6.0 software.
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bioRxiv - Cell Biology 2019Quote: ... The secondary antibodies used for Western blotting were goat ⍰ -mouse IgG HRP conjugate (170-6516 from Bio-Rad; 1:2000) and goat ⍰ -rabbit IgG HRP conjugate (170-6515 from Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was washed three times with T-TBS for 3 x 5 min and incubated for 1 h with 1/1000 solution of horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG antibody (1:1000; Biorad) and washed 3 times with T-TBS and 1 time with TBS ...
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bioRxiv - Neuroscience 2020Quote: ... Goat anti-mouse IgG (H+L) horseradish peroxidase-conjugated secondary antibody (1: 8000; Cat. 170-6516, Bio-Rad, Milan, Italy) was used ...
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bioRxiv - Physiology 2021Quote: ... The secondary antibodies were goat anti-mouse HRP-linked and goat anti-rabbit HRP-linked (Bio-Rad, Hercules, CA, USA) for immunoblotting ...
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated secondary antibodies were diluted 1:10000 and 1:2000 for polyclonal Goat-α-Mouse (Bio-Rad, 170-6516) and Goat-α-Rabbit HRP (Dako ...
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bioRxiv - Immunology 2021Quote: ... Greater than 90% of purity of monocytes was achieved as determined by flow cytometry using a mouse anti-bovine CD14 FITC-labelled antibody (BioRad).
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bioRxiv - Microbiology 2020Quote: ... IL-10) responses were measured using a customized Bio-Plex Pro™ Mouse Cytokine Th1/Th2 Assay kit (Biorad, USA) with the BioPlex 200 platform according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2021Quote: ... The following day the membrane was washed with PBST twice for 10 minutes and incubated with secondary antibodies diluted in the same solution (1:10000 HRP-conjugated anti-mouse or anti-rabbit antibodies (Biorad)) in the same solution at room temperature for 45 minutes ...
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bioRxiv - Genetics 2021Quote: ... Secondary antibodies used were: horseradish peroxidase-conjugated goat anti-rabbit or goat anti-mouse IgG secondary antibody (1:5000, BioRad). Blots were visualized using chemiluminescence (Clarity Max™ ECL ...
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bioRxiv - Cell Biology 2020Quote: ... Goat α-mouse secondary IgG-HRP antibody was used at a 1:5,000 dilution (BioRad Laboratories, Hercules, CA, 170-6516). Phospho-MAPK antibodies were incubated in 1X TBST (10 mM TRIS-HCl pH 8 ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were washed with 1% w/v milk in PBS prior to addition of a secondary goat anti-mouse alkaline phosphatase (ALP) conjugated antibody (STAR117A, BioRad) at 1:1000 dilution in 1% w/v milk in PBS ...
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bioRxiv - Biochemistry 2022Quote: ... HRP-conjugated anti-mouse was incubated for 1 h at room temperature and visualized by enhanced chemiluminescence (Clarity Substrate, Biorad) using Amersham Imager 680.
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bioRxiv - Microbiology 2020Quote: ELISA plates were coated overnight at 4°C with 0.1 µg/mL of mouse anti-human IgG (human CH2 domain with no cross-reactivity to rhesus macaque IgG; clone R10Z8E9; BioRad) and then blocked for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... was used in a 1:2,000 dilution and HRP-conjugated goat anti-mouse secondary antibody was used in a 1:3,000 dilution (172-1011, Bio-Rad). Following gDNA based PCR screening and Western blotting ...
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bioRxiv - Genetics 2022Quote: ... followed by goat anti-mouse IgG (H+L) alkaline phosphatase (AP)-conjugated (1:3000, Bio-Rad Cat# 170-6520, RRID:AB_11125348) antibody or Alexa Fluor 546 goat anti-mouse IgG (H+L ...
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bioRxiv - Microbiology 2019Quote: ... Secondary antibodies used for staining plaque or TCID50 assays were goat anti-mouse horseradish peroxidase and goat anti-rabbit horseradish peroxidase (Biorad).
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bioRxiv - Pathology 2019Quote: ... After incubation of the blots with HRP-conjugated secondary antibodies (1:3000 for goat anti-rabbit or 1:10,000 for goat anti-mouse, Bio-Rad), specific protein bands on the blots were visualized by applying enhanced chemiluminescence reagent Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... Serum was collected at day 49 and anti-M1 titers were measured by ELISA as previously described72 with a goat anti-mouse HRP-conjugated secondary antibody at 1:5000 (BioRad). Immunized and control mice were infected subcutaneously into the flank with 2×107 cfu of the AP1 (n=17 ...