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Citations for Bio-Rad :
1301 - 1350 of 1363 citations for Saporin Goat Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... the resulting dinucleosomes were purified from aberrant or non-ligated mononucleosomes by a second preparative native gel system (Biorad 491 prep cell). In contrast to (Poepsel et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Purified septin complexes were analyzed by SDS-PAGE using 4-20% precast polyacrylamide gels (Mini-PROTEAN TGX Gels from Bio-Rad, 4561095), or hand-casted 12% or 15% Tris-glycine polyacrylamide gels ...
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Actin binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin IIbioRxiv - Biophysics 2022Quote: ... except that the protein was further purified by anion exchange chromatography with an Econo-pac High Q cartridge (BIO-RAD, Hercules, CA) before it was concentrated.
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bioRxiv - Biochemistry 2021Quote: ... The purified proteins were running in 2-D gel electrophoresis and images were taken and analyzed in gel dock(Bio-Rad, USA). The details of protein purification protocols like TCA/Acetone ...
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bioRxiv - Molecular Biology 2019Quote: ... The IgG fraction was purified from hybridoma culture medium by Protein A chromatography on a Profinia™ protein purification system (Bio-Rad) as per manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... Target bands were excised on a blue light transilluminator and purified with a Quantum Prep® Freeze ‘N Squeeze spin column (Bio-Rad). The eluate was further purified with a magnetic bead clean-up (Agencourt® AMPure® XP ...
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bioRxiv - Biochemistry 2020Quote: ... the desalted protein fraction was purified using the Uno S6 prepacked monolith cation exchange column (12 × 53 mm, 6 mL, Bio-Rad, USA). The eluted fraction containing purified bacteriocin was concentrated to 4.6 mg/mL in an Amicon centrifugal filter concentrator with a 3 kDa cutoff membrane (Millipore ...
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bioRxiv - Developmental Biology 2020Quote: ... The purified cDNA was used directly as templates and the qRT-PCR was performed by iTaq Universal SYBR Green Supermix (Bio-Rad, #1725121) and QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1-5 μg of purified antigen or 20 μg protein extract were loaded in a precast 4-20% acrylamide gel (BioRad 456-1094). Gels were run for the first 20-30 min at 40V ...
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bioRxiv - Microbiology 2020Quote: ... and purified from remaining salts using Dowex 50 W X 8 50-100 mesh (Bio-Rad, 10 x 1 cm, H+ form) equilibrated with water ...
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bioRxiv - Genomics 2020Quote: ... Purified DNA was slot blotted onto a Hybond N+ membrane with the help of a dot-blot apparatus (Bio-Rad, 170-6545) and subsequently hybridized with a biotin-labeled (TTAGGG)3 probe synthesized by Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was quantified by nanodrop and cDNA was produced from 1□μg of purified RNA using the iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad Laboratories). qPCR reactions were performed using the SsoFast Universal SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2024Quote: The tRNA modified product from MenT3 in vitro reactions underwent the following steps: it was initially purified using Bio-Spin® 6 Columns (Bio-Rad), followed by incubation with a putative exoribonuclease (10 µM ...
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bioRxiv - Physiology 2024Quote: ... T3 was extracted from supernatants and purified through solid-phase chromatography using 200 – 400 anion exchange chloride resin (Bio-Rad, 140-1251) in Poly-Prep chromatography columns (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2023Quote: ... purified protein fractions were separated with 10%–20% SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry blotter (ATTO ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cDNA templates were obtained from 500 ng of purified RNA using iScript Reverse Transcription Supermix for RT-qPCR (Bio-rad, CA). 1×SYBR Green Master Mix buffer (Takara ...
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bioRxiv - Cell Biology 2020Quote: ... followed by 1◻h incubation at 4◻°C with HRP-conjugated goat anti-mouse (1:5000 dilution in 1% YE in PBS-T, BioRad #170–6516) secondary antibody ...
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bioRxiv - Developmental Biology 2021Quote: ... membranes were incubated in 1:1000 rabbit anti-HSP90 antibody (CST #4874) and 1:5000 goat anti-rabbit HRP secondary antibody (Bio-Rad #170-5046). Signal was revealed using Clarity Max™ Western ECL Substrate (Bio-Rad #170-5062) ...
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bioRxiv - Developmental Biology 2021Quote: ... an affinity purified antibody generated against the SAX-7 cytoplasmic tail [gift of (Chen et al., 2001)] and 1:5000 goat anti-rabbit HRP secondary antibody (Bio-Rad #170-5046). For the loading control ...
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bioRxiv - Developmental Biology 2021Quote: ... membranes were incubated in 1:1000 rabbit anti-HSP90 antibody (CST #4874) and 1:5000 goat anti-rabbit HRP secondary antibody (Bio-Rad #170-5046). Signal was revealed using Clarity Max™ Western ECL Substrate (Bio-Rad #170-5062) ...
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bioRxiv - Cell Biology 2021Quote: ... After three washes (5, 10, 15 minutes) the blot was incubated for 60 minutes with secondary Goat Anti-Rabbit-HRP (Bio-Rad, 1:7000) and Alexa 647 Goat-Anti-Mouse antibodies (Molecular Probes ...
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bioRxiv - Biochemistry 2021Quote: ... A goat anti-rabbit IgG (H+L) and goat anti-mouse IgG (H+L) antibody with HRP conjugate (1706515 and 1706516, respectively, Bio-Rad, Hercules, CA) was used as a secondary detection antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... incubated with 3% H2O2 for 15 min at room temperature and then incubated with goat anti-rabbit IgG-HRP (Bio-Rad 170-6515) secondary antibody diluted 1:300 in the blocking buffer for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Then membranes were washed 3 times with TBS-T and incubated with goat anti-mouse or anti-rabbit IgG HRP-conjugated secondary antibody (1:10000 in 5% BSA TBS-T, Bio-rad; #1706515, #1706516) for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... The slides were then incubated for 60 minutes at room temperature in blocking buffer (PBS with 5 % goat serum [Abcam #ab7481] and 0.3 % Triton X-100 [Bio-Rad Laboratories #161-0407]). After removing the blocking buffer and re-applying PAP pen ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies used were Immun-Star goat anti-rabbit (GAR)-HRP Conjugate (Cat # 170-5046, or Cat#170-6515; Bio-Rad Laboratories, USA). Clarity Western ECL Substrate (cat #170-5061 ...
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bioRxiv - Molecular Biology 2023Quote: ... at room temperature for 1 h with slight shaking and SP-A-S protein or SP-A-RBD complexes were detected by adding HRP-conjugated Goat Anti-rabbit IgG (1:2000, Bio-rad, Hercules, USA) for an additional 1 h ...
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bioRxiv - Microbiology 2024Quote: ... followed by IR800-conjugated goat anti-mouse immunoglobulin (LiCor 926-32210; 0.1 μg/ml) and DyLight680-conjugated mouse anti-rabbit GAPDH antibody (BioRad MCA4739D680; 0.3 μg/ml). Membranes were imaged using a LiCor Odyssey CLx imager.
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bioRxiv - Biophysics 2019Quote: ... The SUMO tag was cleaved by Ulp-1 as described above and the protein was further purified using ion-exchange chromatography (Bio-Rad, Hercules, California). In all purification steps ...
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bioRxiv - Microbiology 2022Quote: His-FhaAMtb was induced with 1mM IPTG at 18°C overnight and purified on Ni-NTA resin (G-Biosciences, 786-940 in 5 ml Bio-Scale Mini Cartridges, BIO-RAD 7324661), then dialyzed ...
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bioRxiv - Cell Biology 2022Quote: ... and 60 μg purified 6xHis-CNAα copurified with regulatory subunit CNB were resolved by SDS-PAGE and transferred to a nitrocellulose membrane (Bio-Rad, 162-0112). The membrane was stained with Ponceau S solution (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... RNA probes were then alkaline hydrolyzed (80mM NaHCO3, 120mM Na2CO3) for 60°C for 15 minutes and purified using a microcolumn (Bio-Rad 732-6223). Probe integrity was assessed by agarose gel and kept at −80°C when not in use.
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bioRxiv - Neuroscience 2020Quote: 15 μg of total protein extract from purified proteins were separated by native electrophoresis using a Tetra cell (Bio-Rad; Hercules, CA, USA), in 12% polyacrylamide separation gel and a 4% polyacrylamide stacking gel in non-denaturing conditions ...
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bioRxiv - Microbiology 2020Quote: A sample of purified basal bodies was run briefly into a 4-20% Mini-PROTEAN® TGX Stain-Free™ gel (BIO-RAD), which was then stained with InstantBlue™ before the gel section containing the sample was excised and sent for analysis at the BSRC Mass Spectrometry Facility (University of St Andrews).
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bioRxiv - Microbiology 2022Quote: ... TRD-less Mod proteins for both ModP and ModQ were then further purified by electroelution (100 mA for 30 min) into 1x MOPS buffer using a mini whole gel eluter (Biorad, Gladesville, NSW, Australia). Protein fractions were collected from each well and analysed by silver staining (Sigma ...
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bioRxiv - Immunology 2022Quote: ... Both anti-IL-9 and the isotype antibody were purified from the culture supernatant of hybridoma cells using the Proteus protein purification spin kit (Bio-Rad, Hercules, CA). Anti-IL-13 is an IgG1 antibody (55 ...
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bioRxiv - Genetics 2023Quote: ... was equilibrated in Milli-Q purified H2O for 10 min and then placed in the Bio-Dot Microfiltration Apparatus (Bio-Rad, 170-6545) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Some of the affinity chromatography-purified proteins were run through a Superdex200 Increase 10/300 GL column integrated in an NGC chromatography system (Bio-Rad, CA, USA) to obtain a profile of the content in different species (aggregates ...
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bioRxiv - Neuroscience 2023Quote: ... Equal volumes of the purified fractions were analyzed on a polyacrylamide gel (4–20% TGX Precast Gels, Bio-Rad, Marnes-la-Coquette, France). Total actin was then determined by immunoblotting actin for each experimental condition ...
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bioRxiv - Genomics 2024Quote: ... 10 ng of purified genomic DNA was added to a reaction consisting of 10 µL of ddPCR Supermix for Probes (Bio-Rad, Cat #1863024), 1 µL of MseI (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... The plate was washed 3 times prior to adding 50 μl of 1:1000 diluted Goat anti-Mouse IgG H+L-HRP antibody (Bio-Rad, Cat# 172-1011) to the plate and incubated for 1 h at RT ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... After overnight incubation membranes were washed three times with 1x TBST for ten minutes per wash and then incubated with a secondary goat anti-rabbit antibody (BioRad Cat#1706515, 1:10000) for one hour with rocking at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed three times with 1X PBS for 5 min and incubated with goat anti-rabbit IgG-alkaline phosphatase-conjugate secondary antibody (Bio-Rad, 1:3000 dilution) for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Antibody binding was detected with goat antihuman IgG conjugated to phycoerythrin and quantified on a Bio-Plex Suspension Array System (Bio-Rad, Hercules, CA, USA). Weighted median fluorescence intensity (MFI ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then washed thrice with TBST (5 minutes/wash) at room temperature and then incubated with HRP-conjugated goat anti-rabbit IgG (H + L) (Bio-Rad, Cat #: 170-6515) or goat anti-mouse IgG (H + L ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR-fragments were gel-purified using Micro Bio-Spin P-30 Gel columns Tris Buffer (RNase-free)(Bio-Rad, Hercules, CA, USA Cat#7326250) and subsequently ...
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bioRxiv - Molecular Biology 2021Quote: Total protein was extracted from SSLs using 350 μL of RIPA buffer and was purified using the Ready Prep 2-D cleanup kit (Bio-Rad, Hercules, CA, USA); the protein concentration was measured using the BCA protein assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... the SL2-6 construct was subsequently size purified using a denaturing polyacrylamide gel (7M Urea, 1x TBE, hand-poured in Bio-Rad Criterion midi cassettes), loaded in 80% formamide ...
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Oligomerization state of the functional bacterial twin arginine translocation (Tat) receptor complexbioRxiv - Biophysics 2021Quote: ... The mNeon genetic dimer (2xmNeon) was also purified via size exclusion chromatography using FPLC buffer (Bio-Rad NGC system with Enrich SEC 75 column).
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bioRxiv - Plant Biology 2020Quote: ... the recombinant protein in the filtrate was further purified by size exclusion chromatography (NGC™ chromatography system with Enrich™ SEC 650 column, BioRad, Munich, Germany) in 50 mM NaH2PO4 /Na2HPO4 ...