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Citations for Bio-Rad :
1051 - 1100 of 1290 citations for Saporin Goat Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The nitrocellulose membrane was washed three times with 1x TTBS and then probed at a 1:20,000 dilution of HRP-conjugated goat α-rabbit antibody (Biorad) in 5% non-fat milk solution for one hour ...
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bioRxiv - Neuroscience 2020Quote: ... Goat anti-mouse IgG (H+L) horseradish peroxidase-conjugated secondary antibody (1: 8000; Cat. 170-6516, Bio-Rad, Milan, Italy) was used ...
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bioRxiv - Physiology 2021Quote: ... membranes were rinsed three-times in TBS/0.2% Tween for 10min and incubated for 1h with goat secondary anti-rabbit IgG linked to peroxidase (dilution 1:5 000, Biorad) in TBS/0.2% Tween/2% milk ...
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bioRxiv - Microbiology 2021Quote: ... Immunoreactive bands were visualized by incubation with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulins (1:5,000, Bio-Rad 170-6515) or goat anti-mouse immunoglobulins (1:5,000 ...
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bioRxiv - Cell Biology 2020Quote: ... Goat α-mouse secondary IgG-HRP antibody was used at a 1:5,000 dilution (BioRad Laboratories, Hercules, CA, 170-6516). Phospho-MAPK antibodies were incubated in 1X TBST (10 mM TRIS-HCl pH 8 ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were washed with 1% w/v milk in PBS prior to addition of a secondary goat anti-mouse alkaline phosphatase (ALP) conjugated antibody (STAR117A, BioRad) at 1:1000 dilution in 1% w/v milk in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... the membranes were incubated with goat anti-rabbit antibody conjugated to HRP (1:500 or 1:1000 against MEK or ERK/GST, respectively) (BioRad) for one hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... was used in a 1:2,000 dilution and HRP-conjugated goat anti-mouse secondary antibody was used in a 1:3,000 dilution (172-1011, Bio-Rad). Following gDNA based PCR screening and Western blotting ...
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bioRxiv - Genetics 2022Quote: ... followed by goat anti-mouse IgG (H+L) alkaline phosphatase (AP)-conjugated (1:3000, Bio-Rad Cat# 170-6520, RRID:AB_11125348) antibody or Alexa Fluor 546 goat anti-mouse IgG (H+L ...
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bioRxiv - Microbiology 2019Quote: ... Blots were probed for FlgR using FlgR antisera raised in rabbit and developed using HRP-conjugated goat-antirabbit secondary antibody with a ChemidocMP imager (BioRad)
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bioRxiv - Molecular Biology 2019Quote: ... Then the membranes were incubated with goat anti-rabbit IgG (H+L) alkaline phosphatase conjugate (1:10,000 dilution) (BIO-RAD). Subsequently ...
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bioRxiv - Microbiology 2019Quote: ... followed by incubation with EndoS34 or SpeB68 antiserum The membranes were washed followed by incubation with a secondary antibody (goat anti-rabbit HRP-conjugated antibody, BioRad). The membranes were developed using Clarity Western ECL substrate (BioRad ...
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bioRxiv - Microbiology 2019Quote: ... Serum was collected at day 49 and anti-M1 titers were measured by ELISA as previously described72 with a goat anti-mouse HRP-conjugated secondary antibody at 1:5000 (BioRad). Immunized and control mice were infected subcutaneously into the flank with 2×107 cfu of the AP1 (n=17 ...
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bioRxiv - Cancer Biology 2020Quote: ... After incubation with the secondary antibody (goat anti-rabbit IgG HRP conjugate, JD111036047) 5mC signal was detected using the ChemiDocTM XRS+ Imaging System (Biorad) and analyzed using Image LabTM Software (Biorad) ...
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bioRxiv - Physiology 2021Quote: ... Membranes were washed with 4 x 15 min TBS-T washes prior to incubation with secondary antibody (goat anti-rabbit-HRP diluted 1:10,000, Bio-Rad) for 1 h on a shaker at room temperature ...
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bioRxiv - Physiology 2020Quote: ... antibody at 4°C overnight and in goat anti-rabbit IgG horseradish peroxidase conjugated secondary (1:5000) antibody for 1 hour before detection using ECL detection (BioRad) and ChemiDoc Imaging (BioRad) ...
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bioRxiv - Molecular Biology 2021Quote: ... membrane was washed repeatedly with PBS-T and incubated 1 h at RT with the following secondary antibody diluted 1/5000 in milk PBS-T: Goat anti-rabbit IgG HRP conjugate (170-6515; BioRad). After the same washing procedure ...
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bioRxiv - Immunology 2021Quote: ... Recombinant Bovine Interleukin-8 PBP039 and Goat anti Bovine Interleukin-8 Ab conjugated to biotin AHP2817B (all from Bio-Rad, protocol according to the manufacturer’s intructions).
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bioRxiv - Microbiology 2021Quote: ... Binding of the anti-NTD antibody was detected using a goat anti-human kappa light chain conjugated to horseradish peroxidase (HPR) (BioRad). To normalize for the amount of the bound probes ...
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bioRxiv - Cell Biology 2022Quote: ... and probed with designated primary antibodies followed by a goat anti-rabbit IgG HRP-conjugated secondary antibody (BioRad, Hercules, CA). Bands were visualized using SuperSignal reagent (Thermo-Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were washed three times and incubated with secondary antibodies: Donkey anti Sheep/Goat IgG (dilution 1:100,000, #STAR88P, Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2022Quote: ... the membrane was incubated 3 h with a solution containing a secondary goat-HRP anti rabbit (1:10 000) (Biorad) (milk 0.5 % TBST) ...
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bioRxiv - Microbiology 2022Quote: ... then incubated for 1 hour at RT with 1:10,000 goat anti-rabbit horseradish peroxidase (HRP) (Bio-Rad, Cat# STAR208P). The membrane was washed 3 times for 5 minutes with TBST ...
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bioRxiv - Microbiology 2022Quote: ... HRP-linked Goat αRat antibody (Pierce 31470) was used at 1:30,000 and detected with an HRP Substrate kit (Bio-Rad) and Bio-Rad ChemiDoc imager ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were rinsed 3x for 15 minutes in 1X TBST and incubated in the proper secondary antibody (Goat anti-mouse BioRad #170-6516 or Goat anti-rabbit BioRad #170-6515 ...
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bioRxiv - Immunology 2023Quote: ... Blocked membranes were incubated with HuCAL anti-bovine Mincle CRD antibodies (2.5 µg mL-1) before incubation with secondary goat-anti-human IgG F(ab’)2: HRP (STAR126P, Bio-Rad) antibody ...
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bioRxiv - Immunology 2023Quote: ... IgA and IgG antibody isotypes were detected by horse reddish peroxidase (HRP)-conjugated goat anti-pig antibodies specific for porcine IgA (clone AAI40P, BioRad) diluted 1:5 000 ...
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bioRxiv - Microbiology 2023Quote: ... This step was followed immediately by an hour incubation with 1:5000 dilution of Goat α-mouse-HRP (Bio-Rad) in TBST ...
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bioRxiv - Microbiology 2023Quote: ... followed by three-times washing with TBST for 5 min and subsequent incubation with the goat anti-rabbit IgG (H+L)-HRP conjugate (BioRad) (diluted 1:3000 in TBST ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated with the following secondary antibody diluted 1/5000 in milk PBS-T: Goat anti-rabbit IgG HRP conjugate (170-6515; BioRad) or Goat anti-mouse IgG HRP conjugate (170-6516 ...
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bioRxiv - Microbiology 2023Quote: ... blots were washed twice in TBS with 0.05% Tween 20 and then incubated with a goat α-rabbit secondary antibody conjugated to horseradish peroxidase (Biorad) at a 1:5 000 dilution in TBS with 0.05% Tween 20 and 5% milk for 60 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The following secondary antibodies and dilutions were used for 1 hour at room temperature: goat anti-rabbit StarBright Blue 700 (1:2,500; Bio-Rad), goat anti-rabbit IRDye 800 CW (1:5,000 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with secondary antibody goat anti-rabbit conjugated to HRP (1:5000, 5% wt/vol milk in TBS-T; #170-6515, BioRad) for 1 h ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... The membrane was rinsed three times with TBS-T and then incubated with goat anti-mouse IgG coupled to horse radish peroxidase (1/10,000, 1706516, Bio-Rad) in TBS-T for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were then washed with TBST and incubated with StarBright Blue 700 goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 h ...
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bioRxiv - Biophysics 2021Quote: ... protein was further purified by a Hydroxyapatite column (HA) connected to an NGC Medium-Pressure Liquid Chromatography System (BIO-RAD). A gradient form 5 mM NaPi ...
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bioRxiv - Immunology 2022Quote: ... Complementary DNA (cDNA) was synthesized from 500 ng of purified RNA using the iScript™ cDNA Synthesis Kit (Bio-Rad) following protocols provided by the manufacturer.
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bioRxiv - Bioengineering 2020Quote: ... DGF-298 electrocompetent cells were transformed with 300 ng of the purified PCR product (described above) by electroporation in a Genepulser XCell (Biorad) at 1800 V ...
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bioRxiv - Biophysics 2021Quote: ... 5 µL of purified (mutant) protein sample was mixed with 1.67 µL of 4X Laemmli loading buffer (Bio-Rad 1610747), then 5 µL was loaded onto an SDS-PAGE gel (Either 12% isocratic (Bio-Rad 4561046 ...
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bioRxiv - Microbiology 2021Quote: ... The purified total RNAs were converted to first-strand cDNAs by using a reverse transcription kit (Bio-Rad, Cat#1708891). The relative levels of HIV-1 un-spliced transcript and human MMP-2 mRNA were measured by qRT-PCR using the primers 5’AGGGACCTGAAAGCGAAAG3’ (HIV-1 un-spliced-forward ...
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bioRxiv - Microbiology 2021Quote: ... purified RNA (1000 ng) was converted to cDNA by using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA) following the manufacturer’s standard procedure ...
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bioRxiv - Cell Biology 2020Quote: Purified ORB proteins were concentrated in a stacking gel using SDS-PAGE stained with Coomassie Brilliant Blue R-250 (Biorad). Proteins in the excised gel band were subjected to in-gel digestion as follows ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized from 1 μg of purified RNA using the iScript cDNA synthesis kit (Cat. no. 1708891, Bio-Rad). Quantitative real-time PCR assays were performed using the iQ SYBR Green Supermix (Cat ...
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bioRxiv - Biophysics 2020Quote: ACE2-hFc and CR3022 neutralizing antibody binding studies with the various RBD derivatives purified from different expression platforms were carried out using the ProteOn XPR36 Protein Interaction Array V.3.1 from Bio-Rad. Activation of the GLM sensor chip was performed by reaction with EDC and sulfo-NHS (Sigma) ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA samples were fragmented for 10 minutes on ice in 0.2 N NaOH and purified on Bio-Spin P30 columns (Bio-Rad). Biotin-labelled RNA was then purified using MyOne Streptavidin C1 Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Transcripts and labelled oligonucleotides to be used as a size indication were purified using P-30 Bio-Gel spin columns (Biorad). All reactions were stopped in gel loading buffer II (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Purified libraries were diluted 1:100 and quantified again by qPCR (Two 25uL reactions, 2x iQ SYBR SUPERMix (Bio-Rad, with Read 1 (5’-TATGGTAATTGT GTGYCAGCMGCCGCGGTAA-3’) ...
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bioRxiv - Physiology 2021Quote: ... we ran the purified Hb fractions on a mini-protean precast 4-20% SDS PAGE gel (Bio-Rad, Hercules, CA), which we then stained with Coomassie brilliant blue-G ...
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bioRxiv - Cell Biology 2019Quote: ... and the supernatants were purified with IBA Strep-Tactin sepharose beads (IBA, 2-1201-002) on Poly-Prep Chromatography Columns (731-1550, BioRad) with IBA’s buffer’s W (washing ...
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bioRxiv - Synthetic Biology 2019Quote: DNA origami samples were either purified with size exclusion chromatography in hand-packed Micro Bio-Spin™ Columns (Bio-Rad) loaded with 750 μl of Sephacryl™ S-300 HR beads (GE Healthcare) ...