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Citations for Bio-Rad :
951 - 1000 of 1290 citations for Saporin Goat Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and the goat anti-mouse IgG-HRP conjugate (catalog number #A-10668) was also acquired from Bio-Rad in Mississauga ...
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bioRxiv - Physiology 2021Quote: ... Protein concentration in purified microsomes and RyR1 fractions was measured with Quick Start Bradford Protein Assay (Bio-Rad). The plasmid encoding for MSP1E3D1 ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR product was purified by preparative agarose gel electrophoresis (Quantum Prep Freeze ‘N Squeeze spin column, Bio-Rad) and magnetic beads (Agencourt AMPure XP ...
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bioRxiv - Molecular Biology 2020Quote: Purified protein was analyzed via SDS-PAGE under reducing conditions using an AnykDa precast gel (Bio-Rad, USA) in a Mini Protean Tetra Cell device (Bio-Rad ...
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bioRxiv - Biophysics 2020Quote: ... we purified the DNA FluoroCubes by extraction and centrifugation in Freeze ’N Squeeze columns (BioRad Sciences, 732-6165). Prior to extraction ...
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bioRxiv - Microbiology 2022Quote: ... Purified virions were separated on a 4 - 20% Mini-PROTEAN TGX stain-free protein gel (Bio-Rad, USA). The gel was transferred on a polyvinylidene fluoride membrane and blocked with 5% BSA at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was purified by loading quenched samples onto Micro Bio-spin Columns with Bio-Gel P-6 (BioRad), followed by ethanol precipitation ...
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bioRxiv - Microbiology 2021Quote: ... combined with 10 μl of purified DNA in a 0.1 cm Gene Pulser cuvette (Bio-Rad, cat#1652083) and electroporated on an Electroporator 2510 instrument (Eppendorf ...
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bioRxiv - Microbiology 2020Quote: ... Purified NahA was analyzed by SDS-PAGE and the concentration was measured by the Bradford assay (Bio-rad).
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bioRxiv - Immunology 2022Quote: ... qRT-PCR was performed on the purified DNA with the iTaq Universal SYBR® Green Supermix (Bio-Rad) on a QuantStudio 6 Flex System (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were purified by a combination of chromatographic steps by NGC Scout Medium-Pressure Chromatography Systems (Bio-Rad). The bacterial cells were centrifuged at 6,000 rcf for 15 min at 4 °C ...
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bioRxiv - Biochemistry 2019Quote: Complementary DNA was synthesized from 1 μg of purified RNA using the iScript cDNA Synthesis Kit (BioRad, USA) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... the concentration of purified scCro was determined using the Quick Start™ Bradford Protein Assay Kit (Bio-Rad) and the sample was stored at -25 °C (Figure S3).
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bioRxiv - Microbiology 2021Quote: ... The protein was purified from the lysate of the bacterial cells by NGC Chromatography system (BioRad, CA, USA) with nickel column ...
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bioRxiv - Biochemistry 2020Quote: ... Purified proteins were supplemented in 50% glycerol and were quantified using the Bradford protein assay kit (Bio-Rad). Proteins were aliquoted and stored at −80°C until needed ...
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bioRxiv - Immunology 2019Quote: ... purified PR3 mutant proteins were loaded (1 µg/lane) onto 12% Tris-HCl gels from BioRad (Hercules, CA). The SDS gel electrophoresis was performed at 180 volts for 35 minutes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Total RNA was purified from homogenized SKM and BAT tissues using PureZOL™ RNA Isolation reagent (BioRad, CA) as described [23,24] ...
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bioRxiv - Biochemistry 2021Quote: ... PCR product was purified by preparative agarose gel electrophoresis (Quantum Prep Freeze ‘N Squeeze spin column, Bio-Rad) and magnetic beads (Agencourt AMPure XP ...
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bioRxiv - Immunology 2020Quote: ... Purified trimeric SOSIP proteins were analyzed in a gradient 4-15% BN-PAGE (Mini-PROTEAN TGXTM, Bio-Rad). The degree of 1PGE-THIVC cleavage was examined by incubating the SOSIP protein with 0.1 M dithiothreitol (DTT ...
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bioRxiv - Cell Biology 2020Quote: Total cell lysates and purified sumoylated proteins were resolved by SDS-PAGE using 4-20% gradient gels (BioRad). Western analyses were performed with mouse anti-FLAG (1:2500 ...
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bioRxiv - Cell Biology 2022Quote: Purified γ-TuRC preparations (GCP3- and GCP6-tagged) were resuspended in 20 μL of Laemmli Sample buffer (Biorad) and were loaded on a 4%–12% gradient Criterion XT Bis-Tris precast gel (Biorad) ...
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bioRxiv - Microbiology 2022Quote: ... The purified 3-cycle PCR products were qPCR quantified using Platinum SuperFi II on a CFX96 system (BioRad). The same amount of the seven purified 3-cycle PCR products were further amplified using Platinum SuperFi II and the index primers from Nextera XT Index Kit v2 (Illumina cat ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were purified by a combination of chromatographic steps by NGC Scout Medium-Pressure Chromatography Systems (Bio-Rad). The bacterial cells were centrifuged at 6,000 rcf for 15 min at 4 °C ...
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bioRxiv - Genetics 2023Quote: Precipitated DNA (without glycogen) was resuspended in water and further purified using Micro Bio-Spin columns (Bio-Rad), after which half of the DNA was oxidised with 15 mM KRuO4 (Alpha Aesar ...
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bioRxiv - Biochemistry 2022Quote: Purified protein fractions were separated on a Mini-PROTEAN TGX Stain-Free protein gel (Bio-Rad Laboratories, Inc.). Separated protein bands were visualized using “Stain Free Gel” application mode on ChemiDoc MP Imaging System (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins in the cleared lysate were purified using Profinity™ IMAC Nickel Charged Resin (Bio-Rad) and eluted in 50 mM sodium phosphate buffer with 300 mM NaCl pH 8 and 300 mM imidazole (Fischer Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... Purified RNA (1 μg) was used for reverse transcription using the iScript™ cDNA Synthesis Kit (BIO-RAD). RT-qPCR was performed using SYBR green (ThermoScientific ...
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bioRxiv - Neuroscience 2024Quote: ... Generated probes were purified using Micro Bio-SpinTM P-30 Gel Columns with RNase-free Tris Buffer (BioRad) according to the manufacturer’s protocol and stored at −80°C ...
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bioRxiv - Genetics 2021Quote: ... The secondary antibody used with all three primary antibodies was HRP-conjugated goat anti-rabbit (Bio-Rad cat#1662408EDU). Blots were imaged on a SynGene gel imaging system after a 1min exposure to SuperSignal Femto West Maximum Sensitivity Substrate (ThermoFisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were incubated in 5% BLOT-QuickBlocker as follows: goat anti-mouse HRP conjugate (BioRad, 1706516; 1:5000), donkey anti-rabbit HRP conjugate (Amersham ...
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bioRxiv - Plant Biology 2019Quote: ... the membrane was incubated with the secondary antibody: the goat antirabbit IgG (H + L)-horseradish peroxidase conjugate (Bio-Rad). Blots were visualized by an Amersham Imager 600 gel scanner (GE HealthCare Life Sciences ...
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bioRxiv - Microbiology 2019Quote: ... Immunoreactive bands were visualized by incubation with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulins (1:5000, BioRad 170-6515) or goat anti-mouse immunoglobulins (1:5000 ...
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bioRxiv - Physiology 2021Quote: ... membranes were rinsed three-times in TBS/0.2% Tween for 10min and incubated for 1h with goat secondary anti-rabbit or anti-mouse IgG linked to peroxidase (dilution 1:5,000; Biorad) in TBS/0.2% Tween/2% milk ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-syntenin-1 (SCBT, sc-515538) and horseradish peroxidase-conjugated secondary goat anti-mouse (Bio-Rad, 170-6516), goat anti-rabbit (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: Immunoreactive bands were visualized by incubation with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulins (1:5000, BioRad 170-6515) or goat anti-mouse immunoglobulins (1:5000 ...
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bioRxiv - Microbiology 2020Quote: ... membranes were blocked overnight in PBST supplemented with 5% non-fat dry milk and incubated with antiserum (1:5,000) or vaginal washes (1:500) from test or control mice followed by goat anti-mouse IgG (BioRad) or IgA (SouthernBiotech ...
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bioRxiv - Microbiology 2022Quote: ... kind gift by Wolfram Brune) followed by a secondary antibody Goat Anti-Mouse IgG StarBright Blue 700 (Bio-Rad). The stained blots were imaged using a ChemiDoc MP imager (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... The plate was then washed two times with TBST and then incubated with a 1:5,000 dilution of secondary antibody (Goat Anti-Rabbit IgG (H+L)-HRP (BioRad)) for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... The plate was then washed with TBST and then incubated with a 1:10,000 dilution of secondary antibody (Goat Anti-Rabbit IgG (H+L)-HRP (BioRad)) for 1 hour ...
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bioRxiv - Microbiology 2019Quote: ... Blots were incubated with appropriate horseradish peroxidase -conjugated secondary antibody goat anti-rabbit immunoglobulins (1:5000, BioRad 170-6515) or goat anti-mouse immunoglobulins (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... followed by incubation with EndoS or SpeB rabbit antiserum 12,68 another wash and incubation with a secondary antibody (goat anti-rabbit HRP-conjugated antibody, BioRad). The membranes were developed using Clarity Western ECL substrate (BioRad ...
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bioRxiv - Physiology 2021Quote: ... The membranes were further washed in TBST buffer before adding secondary antibody (Bio-Rad, Goat-anti-rabbit, #170-6515) in 1% BSA (or 1% milk for SWELL1 ...
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bioRxiv - Microbiology 2020Quote: ... Western blots were resolved using an HRP-conjugated goat anti-rabbit secondary antibody and Western ECL substrate (Bio-Rad).
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bioRxiv - Genetics 2020Quote: ... or by anti-Smc3 K112/K113 Acetylation (1:1,000, gift from Dr. Katsuhiko Shirahige) in combination with goat anti-mouse HRP (1:10,000) (Bio-Rad) and ECL prime (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... Bound PRRSV-2-specific IgG was detected using goat anti-pig IgG conjugated with horseradish peroxidase (HRP; Bio-Rad) diluted at 1:10,000 in blocking buffer for 1 h at RT ...
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bioRxiv - Immunology 2022Quote: ... Membranes were washed and incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-mouse secondary antibody (Bio-Rad) or goat anti-rabbit secondary antibody (Abcam) ...
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bioRxiv - Neuroscience 2022Quote: ... and Goat anti-rabbit Igg (H+L) horseradish peroxidase-conjugated secondary antibody (Bio-Rad, 1:5000; Cat. 170-6515,) were used as secondary antibodies ...
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bioRxiv - Genomics 2022Quote: ... membranes were rinsed threetimes in TBS/0.2% Tween for 10min and incubated for 1h with goat secondary anti-rabbit IgG linked to peroxidase (dilution 1:5,000; Biorad) in TBS/0.2% Tween/2% milk ...
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bioRxiv - Plant Biology 2022Quote: ... USA (17) and a 1:3000 dilution of goat anti-rabbit IgG horseradish peroxidase (HRP) conjugate secondary antibody (Biorad). Chemiluminescence was detected using WesternBright ECL (Advansta ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed with TBST and incubated with StarBright Blue 700 Goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 hour ...