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Citations for Bio-Rad :
1251 - 1287 of 1287 citations for Warfarin Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... fractions with Afl RT were pooled and loaded onto a 2-ml Macro-Prep DEAE Resin (Bio-Rad, column CA, Hercules, USA) pre-equilibrated with a buffer C (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were washed three times for 5 min each with TBST and incubated with 1 mL of chemiluminescent detection reagent (Cytiva, RPN2232) for 3 min before image acquisition using a ChemiDoc MP Imaging System (Bio-Rad Laboratories). Horseradish peroxidase (HRP)-conjugated antibodies against β-actin (Cell Signaling ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were washed three times for 5 min each with TBST and incubated with 1 mL of chemiluminescent detection reagent (Cytiva, RPN2232) for 3 min before image acquisition using a ChemiDoc MP Imaging System (Bio-Rad Laboratories).
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bioRxiv - Biophysics 2024Quote: ... we collected the supernatant in a new 1.5 ml reaction tube and determined the protein concentration of each sample by a Bradford protein assay (Biorad, Cat. No.: 5000006). For each sample ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The clarified lysate was loaded with a peristaltic pump (Hei-FLOW 06, Heidolph, Germany) on prepacked nickel-NTA columns (5 mL Nuvia IMAC Ni-Charged, Biorad, USA-CA) that were equilibrated before with buffer A ...
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bioRxiv - Microbiology 2022Quote: ... and collecting 1 mL fractions from the top at a pace of 1 mL per minute while continuously measuring A254 using the BioLogic LP system (BioRad, Hercules, California, USA).
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bioRxiv - Microbiology 2019Quote: One hundred microlitres of recombinant ZIKV NS1 antigen (ensuring at an optimal protein concentration range of 0.5-5.0mg/ml) were coupled to 100μg of lyophilized HRPO mix using the LYNX Rapid HPR Conjugation kit (Bio-Rad Laboratories Ltd, Watford, UK) in accordance with the manufacturer’s instructions Once conjugated the product was diluted 1 in 10 in HRP Stabilising Buffer (ClinTech ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Antigen expression was confirmed on ice-cold acetone fixed 8 μm cryostat sections of C51 and F9 tumors stained with IgG (L19)-FITC (final concentration 2 μg/mL) and detected with a Rabbit anti-FITC (Bio-Rad; 4510-7804) and Donkey anti-Rabbit AlexaFluor 488 (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... Wells were filled with E3 containing 0.28ug/mL Tricaine-S and the plate sealed with a PCR microseal (BioRad Microseal ‘B’ Film, #MSB1001). Up to 45 larval zebrafish per experiment were imaged in parallel on a GE IN Cell Analyzer 6000 every 15-20 minutes for 24-30h ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was centrifuged at 20,000 x g for 20 min and the cleared lysate was passed through a 0.45-µm filter and loaded onto a XK-26/20 column (Cytiva) with 30-35 ml ceramic hydroxyapatite (Bio-Rad, cat no. 1582000). PlyCBWT was eluted from the column with three column volumes of 1 M sodium phosphate buffer (pH 7.2) ...
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bioRxiv - Microbiology 2020Quote: ... was electroporated into cells at 5×106 cells/0.4 ml under conditions of 220V and 950 µF using a Gene Pulser™ (Bio-Rad, Hercules, CA) and plated on DMEM containing 10% FBS.
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bioRxiv - Biophysics 2020Quote: ... Western blot analysis was performed to determine 4-mL fractions with active A2AR collected with a BioFrac fraction collector (BioRad; Hercules, CA, USA), which were then concentrated through a 30-kDa MWCO centrifugal filter (Millipore ...
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bioRxiv - Microbiology 2022Quote: His-FhaAMtb was induced with 1mM IPTG at 18°C overnight and purified on Ni-NTA resin (G-Biosciences, 786-940 in 5 ml Bio-Scale Mini Cartridges, BIO-RAD 7324661), then dialyzed ...
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bioRxiv - Neuroscience 2022Quote: ... The embryoid media was aspirated and 15 to 20 embryoid bodies were plated per well in 6-well plates and cultured in 3 mL hematopoetic medium (2 mM GlutaMax, 1x Anti-Anti, 55 mM 2-mercaptoethanol (BioRad; Cat. No. 1610710), 100 ng/mL M-CSF ...
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bioRxiv - Genetics 2022Quote: ... a small implant of the thallus of interest (0.5 cm2) was set in 2 mL tube containing 500 μL H2O and ground using a FastPrep (TeSeE - Biorad, Hercules, California, United States) (20s ...
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bioRxiv - Zoology 2019Quote: ... were used within one month of collection to determine total and host protein (mg mL−1) by the Bradford assay (Bradford 1976) against bovine serum albumin standards (Bio-Rad 500-0207). Readings were taken at 595 nm (EnSpire microplate reader MLD2300).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... PPIases and PPIase fragments were vacuum aspirated onto a nitrocellulose membrane as serial dilution starting with 1 µg/ml using the dot blot system (Bio-Rad, Feldkirchen, Germany). Recombinant C3 toxin from C ...
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bioRxiv - Microbiology 2021Quote: All melt-MAMA PCRs were performed on a CFX96 Real-Time PCR Detection System using white hard shell 96-well PCR plates or 0.2 mL 8-tube PCR strips (Bio-Rad, Gladesville, NSW, Australia), and PCR results examined using the CFX Maestro v4.1.2433.1219 software (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ELISA plates were coated with 50 µL/well of 5 µg/mL of mouse anti-bovine IFN-γ monoclonal antibody (mAb) (CC330, Bio-Rad Antibodies) by overnight incubation at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... 50 mg of powder was transferred into a 1.5 mL microcentrifuge tube with 150 ul of 2 x Laemmli buffer (Bio-Rad, Hercules, CA, USA). The sample was immediately vortexed for 1 min followed by incubation at 70°C for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... and 5 U/mL aprotinin) and the protein concentrations in the lysates were determined using a BioRad protein assay kit (BioRad Laboratories, Hercules, CA). Protein samples were subjected to SDS-PAGE ...
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bioRxiv - Immunology 2023Quote: IgE receptor cross-linking (IgECL) on HSMCs was accomplished through sensitization with 1 μg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA) for 24 hours ...
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bioRxiv - Immunology 2023Quote: Extracted whole-kidney protein was diluted in PBS to 900 µg/mL and subjected to a Bio-Plex Mouse Cytokine 23-plex Assay (#M60009RDPD, Bio-Rad, Hercules, CA), according to the manufacturer instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Mixed samples were immediately centrifuged at 200 x g for 5 mins and 20 μl of the supernatant was transferred into 1 ml Bradford reagent (Bio-Rad, CA, USA) in 1.5 ml tubes ...
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bioRxiv - Microbiology 2023Quote: ... The remaining flow through was incubated overnight with 1 mL Strep-Tactin® Sepharose® beads (iba-lifesciences) equilibrated with Binding Buffer and applied to a Poly-Prep chromatography column (Bio-Rad). Flow through was run twice on the column ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was incubated with amylose resin for 1 h at 4°C and loaded onto a 20 ml chromatography column (Bio-Rad, Hercules, CA, USA). After washing 3 column volumes with wash buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were electrophoresed in a 1% agarose gel containing 0.5 μg/mL ethidium bromide at 150V for 40 minutes and imaged in a ChemiDoc™ Touch Imaging System (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Biophysics 2022Quote: ... The conformational homogeneity of RNA sample was checked with an ENrich™ SEC 650 10 x 300 Column (Bio-Rad; column volume: 24 mL). RNA (20 μM ...
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bioRxiv - Genetics 2022Quote: ... of each mating type of the strains of interest were placed in 2 mL tubes containing 500 μL H2O and ground using a FastPrep (TeSeE - Biorad, Hercules, California, United States) (20s ...
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bioRxiv - Cell Biology 2022Quote: ... the sample was further purified and Iodixanol was removed by size exclusion chromatography using a 20 ml column (Bio-Rad Laboratories, Hercules, CA, USA) packed with 20 ml Sephadex g 100 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... The total protein concentration of pooled saliva samples was measured to be approximately 20 mg/mL using the Bradford assay kit (Bio-Rad, Hercules, CA, USA) and bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2023Quote: ... Optiprep was removed during size exclusion chromatography of the pooled EV-containing fractions using a 20 ml column (Bio-Rad Laboratories, Hercules, CA, USA) packed with 15 ml Sephadex g100 (Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: ... washed twice with 20 ml wash buffer 1 (= lysis buffer + 20 mM imidazole) and then at room temperature packed into a 10 ml Polyprep Chromatography Column (Bio-Rad Laboratories, Feldkirchen, Germany). The protein was eluted from the column with 5 ml elution buffer 1 (= lysis buffer + 500 mM imidazole) ...
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bioRxiv - Biophysics 2019Quote: ... Optiprep was exchanged for PBS by using size exclusion chromatography on the EV-containing fractions pooled in a 20 ml column (Bio-Rad Laboratories, Hercules, CA, USA) packed with 15 ml Sephadex g100 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: Cell lysates in 1X RIPA buffer were mixed with an equal volume of 2X Laemmli sample buffer (Bio-RAD, containing 50 µl/ml 2-mercaptoethanol) followed by boiling for 5 minutes ...
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bioRxiv - Immunology 2020Quote: ... were coated with 0.1 μg/ml of low endotoxin anti-CD3 and anti-CD28 antibodies (clone UCHT1, clone YTH913.12; Bio-Rad AbD Serotec Limited, Hercules, CA, USA) or with PBS alone for 20 hrs at 4°C followed by washing thrice in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... was resuspended in 1 ml of PBS/Ca+/Mg+ buffer and protein content was quantified by DC protein assay kit (Bio-Rad Laboratory, Hercules, CA, USA). 500 µg of synaptosomes were incubated 60 min at 4 °C with sulfo-NHS-biotin (1.5 mg/ml ...