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Citations for Bio-Rad :
901 - 950 of 1287 citations for Warfarin Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... The slurry was applied to a 10-mL Poly-Prep chromatography column (Bio-Rad, USA), which was then washed with 10 mL of purification buffer B (purification buffer A with 50 mM imidazole) ...
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bioRxiv - Biochemistry 2020Quote: ... the beads was transferred to a gravity-flow Econo column (49 ml volume; Bio-Rad) and washed with 2 column volumes (CV ...
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bioRxiv - Bioengineering 2019Quote: ... generated droplets or Gelbeads were extracted into PCR tubes (0.2 mL individual PCR tubes, BioRad) and incubated in a thermal cycler (T100 ...
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bioRxiv - Biophysics 2022Quote: ... lysate-resin suspension was loaded onto a 20 ml polypropylene gravity-flow column (Bio-Rad). After collecting the flow through ...
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bioRxiv - Biophysics 2022Quote: ... lysate-resin suspension was loaded onto a 20 ml polypropylene gravity-flow column (Bio-Rad). After collecting the flow through ...
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bioRxiv - Biochemistry 2022Quote: ... Detergents were removed by the sequential addition of 60 mg/mL Biobeads SM2 (Bio-Rad) for three times within 3 h ...
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bioRxiv - Microbiology 2022Quote: ... SasG-His6 was then purified using a pre-packed 5 ml IMAC cartridge (Bio-rad) on a Bio-rad FPLC ...
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bioRxiv - Microbiology 2022Quote: ... Membrane pellets were resuspended at 11 mg/ml (as determined by Bradford assay, Bio-Rad) in 20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2024Quote: ... at 4 °C for 30 min in a 10 ml poly-prep column (Bio-Rad). The column was then stabilized for 10 min at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Detergents were removed by incubation with 0.8 mg ml-1 Bio-Beads SM2 (Bio-Rad) overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Detergents were removed by incubation with 0.8 mg ml-1 Bio-Beads SM2 (Bio-Rad) overnight at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... UCP1 was detected using a mouse anti-pentahistidine tag:HRP 0.2 μL/mL (MCA5995P – Bio-RAD). VDAC1 Porin was used as a mitochondrial loading control and detected by a mouse anti-VDAC1 0.1 μL/mL (16G9E6BC4 - Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Quantification of cell number per ml was measured on a TC20 Cell Counter (Bio-Rad).
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bioRxiv - Synthetic Biology 2023Quote: ... with 0.2 ml Flat PCR Tube 8-Cap Strips (Bio-Rad™, optical, ultraclear, #TCS0803). Optimum amplification conditions were determined for each by amplifying six concentrations of a 5× dilution series of MG1655 pJK14-Tn4rev pZA31-mCherry-tnpB using two-step amplification with a thermal gradient of 55–75°C ...
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bioRxiv - Physiology 2023Quote: ... Matching slides per animal were immunofluorescence (IF) stained for cd68 (5 µg/mL, MCA1957, BioRad) followed by donkey anti-rat CF750 (2 µg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... before rinsing quickly with 2x10 mL TBS before treating with clarity ECL substrate (Bio-Rad). Signals were detected using a Bio-Rad ChemiDoc Imaging System ...
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bioRxiv - Biochemistry 2023Quote: ... To a solution of Affi-Gel 10 (Bio-Rad, 2 ml, 0.03 mmol, 1.0 eq) in a solid phase synthesis tube with frit was added a solution of compound 11 (27.7 mg ...
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bioRxiv - Microbiology 2023Quote: ... Protein-bound resin was then applied to a 10-mL chromatography column (BioRad, Hercules, CA) and washed twice with 10 mL wash buffer (50 mM NaH2PO4 ...
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bioRxiv - Cell Biology 2024Quote: ... qPCR reactions were prepared using 5 ml SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, USA) and 4 ml 1:6 diluted cDNA template along with 200 nM forward and reverse primers in a total volume of 10 ml per reaction ...
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bioRxiv - Cell Biology 2024Quote: ... and cell density (cells/ml) was measured by an automated cell counter (TC20, Bio-Rad). The culture was diluted by medium lacking nitrogen and carbon sources to a density of 1×106 cells/ml ...
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bioRxiv - Cell Biology 2024Quote: ... then incubated with PBST containing 1% BSA and 22.5 mg/mL glycine (Bio-Rad 1610718) for 30 minutes at 23°C ...
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bioRxiv - Biochemistry 2024Quote: ... The eluent was then applied to a 10-mL self-assembled HAP column (Bio-Rad), washed with 50 mL resuspension buffer ...
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bioRxiv - Neuroscience 2021Quote: ... and heated at 100 °C for 10 min before loading into 12% Mini-PROTEAN TGX Stain-Free gels (Bio-Rad Laboratories, Hercules, CA). Separated proteins were transferred onto nitrocellulose membranes (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2019Quote: Mouse patellofemoral joints and hearts were homogenized using an electric homogenizer with toothed blades in tissue lysis buffer (Machery-Nagel, 740962) containing beta-mercaptoethanol (1:100) (Bio-Rad, 161-0710) and Triton-X-100 (1:100 ...
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bioRxiv - Neuroscience 2019Quote: ... Suspension array analyte concentrations were determined using a Bio-Rad Bio-Plex System Luminex 100 and Bio-Plex manager 5.0 (Bio-Rad Laboratories, Hercules, CA)40 ...
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bioRxiv - Bioengineering 2021Quote: ... The obtained DNA extract (100 μL) was stored at −20 °C until further investigation and subjected to qPCR analysis using 96-well plates (Bio-Rad, Hercules, USA), the Thermocycler CFX connect system (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Samples were boiled at 100°C for 5 min prior to SDS-PAGE separation on 12% acrylamide Mini-protean TGX precast gels (Bio-Rad Laboratories, US) and Western blot analysis.
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bioRxiv - Cell Biology 2019Quote: ... cells from a 10 cm dish were pelleted and lysed with 30 µl lysis buffer (20 nM Tris-HCl, 150 mM NaCl, 1% Triton X-100 (Bio-Rad, Hercules, CA), 20 mM beta-glycerol phosphate and protease inhibitors cocktail ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... A total of 100 μg brain lysate was run on a Bio-Rad Mini-Protean TGX 4-20% polyacrylamide gel (4561094, Bio-Rad, Hercules, CA) at 200V for approximately 40 minutes ...
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bioRxiv - Immunology 2020Quote: ... plates were washed 5 times with 200 μl of 1X PBS per well and developed with 100 μl of TMB per well (Bio-Rad, #R8/R9) for 30 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... A WT culture synchronized for 5% ring stages was loaded with 100 µg vector in transfection buffer via electroporation (310 V, 950 µF, 10 ms; Bio-Rad gene-pulser) as described (Ngwa et al. ...
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bioRxiv - Synthetic Biology 2019Quote: ... The protein pellet was resuspended in 100 mM ammonium bicarbonate (AMBIC) with 20% methanol and quantified by the Lowry method (Bio-Rad DC assay). A total of 100 µg of protein was reduced by adding tris(2-carboxyethyl)phosphine (TCEP ...
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bioRxiv - Microbiology 2022Quote: ... TRD-less Mod proteins for both ModP and ModQ were then further purified by electroelution (100 mA for 30 min) into 1x MOPS buffer using a mini whole gel eluter (Biorad, Gladesville, NSW, Australia). Protein fractions were collected from each well and analysed by silver staining (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... was loaded onto a 10% SDS-PAGE gel and separated at 100 V for 2 h using a Mini Protean® Tetra Cell system (Bio-Rad, USA). Proteins were electro-transferred onto Immobilon TM PVDF membranes (Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... The slides were then incubated for 60 minutes at room temperature in blocking buffer (PBS with 5 % goat serum [Abcam #ab7481] and 0.3 % Triton X-100 [Bio-Rad Laboratories #161-0407]). After removing the blocking buffer and re-applying PAP pen ...
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bioRxiv - Molecular Biology 2024Quote: The synthesis of cDNA was done from 100 ng RNA in 20 μl total reaction volume using iScript Reverse Transcription Supermix kit (Cat# 1708841, Bio-Rad, Hercules, USA), without any changes from themanufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Then 300 ng of plasmids were transformed into 100 μl of competent cells using the bacteria program on the MicroPulser Electroporator (Bio-Rad, CA, USA). After 1 h recovery culture at 37°C in 500 μl of LB ...
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bioRxiv - Cancer Biology 2024Quote: ... PAGE gels were run at 130V for ∼1h in Bis-Tris buffer (GeneScript) and then wet transferred to a 0.2 µm PVDF membrane (BioRad, activated in 100% methanol) at 100V for ∼1 h in 1X transfer buffer containing 20% methanol ...
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bioRxiv - Neuroscience 2023Quote: ... and separated in polyacrylamide gels at 100-140 V on ice (4-15%, Mini-PROTEAN TGX, Bio-Rad; or as described previously [12]). To detect protein-bound acrolein in spinal cords ...
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bioRxiv - Genetics 2023Quote: ... Co-IP reactions were rotated overnight at 4°C with 100 µl of SureBeads Protein G Magnetic Beads (BioRad; Catalog# 1614013; Hercules, CA). The bead lysates were washed five times in the co-IP buffer using a DynaMag Magnet (ThermoFisher ...
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ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal α-motoneuronsbioRxiv - Neuroscience 2023Quote: ... Gels were run in MOPS buffer at 150 V for 1 hour and 15 min and the proteins transferred for 30 min at 100 V on Millipore PVDF membranes (BIORAD Criterion Blotter apparatus). Membranes were blocked for 1 hour at RT with 4% non-fat dry milk (NFDM ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were resuspended in a final volume of 100-200 μL PBSB and analyzed on a flow cytometer (Bio-Rad, ZE5 cell analyzer).
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bioRxiv - Immunology 2024Quote: ... The suspended cells were then diluted 1:100 in PBS and counted using a TC20 automated cell counter (Bio-Rad Laboratories, Hercules CA). For the first vaccination with cda1Δ2Δ3Δ (given to CBA/J ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were separated in sodium dodecyl sulfate – 8% polyacrylamide gel by electrophoresis at 100 V for 2 h (Bio-Rad, Hercules, CA, USA). Wet transfer to a PVDF membrane (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were separated by SDS-PAGE electrophoresis at 100 volts (V) for 1 h on 4–20% Criterion™ TGX Stain-Free™ Protein Gels (1656001, Bio-Rad) and 1x SDS-page running buffer (192 mM glycine ...
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bioRxiv - Microbiology 2024Quote: ... 40 µL of the cell solution with 1 µL DNA (100 ng/µL in Tris-Buffer) was added to a chilled cuvette and pulsed using a Bio-rad Genepulser (Bio-Rad, CA, USA) for 5.7 ms (2000 V ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cell aliquots were thawed on ice for 5 minutes before adding 100 ng of plasmid DNA and electroporated on Bio-rad Micropulser (Bio-Rad, Hercules, CA) using EC2 protocol (0.2 cm cuvette ...
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bioRxiv - Cell Biology 2020Quote: ... column at a flow rate of 4 ml/min using an NGC chromatography system (Bio-Rad) with a dedicated sample pump ...
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bioRxiv - Genetics 2020Quote: ... in 11 parallel transformations (1 mL each) on a Bio-Rad MicroPulser (Bio-Rad, #165-2100). The parallel transformations were combined and mixed with a total of 9.5 mL of recovery medium ...
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bioRxiv - Biochemistry 2021Quote: ... the detergent was removed by adding 0.5 g mL-1 (w/v) Bio-Beads (Bio-Rad) overnight at 4°C ...