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Citations for Bio-Rad :
751 - 800 of 1287 citations for Warfarin Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... a standard solution was prepared with 2.5 mL of 40% Acrylamide (Biorad, 1610140), 604 µL 2% bis-Acrylamide (Biorad ...
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bioRxiv - Cell Biology 2023Quote: ... a standard solution was prepared with 1.25 mL of 40% Acrylamide (Biorad, 1610140) 583 µL 2% bis-Acrylamide (Biorad ...
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bioRxiv - Microbiology 2024Quote: ... sheep polyclonal anti-GFP (Bio-Rad, 4745-1051, 1:1000, 5 µg/mL), rabbit polyclonal anti-GFP (Novus Biologicals ...
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bioRxiv - Microbiology 2024Quote: ... The incubations with 40 mg/mL Blotting-Grade Blocker (BioRad, Hercules, CA, USA) as blocking buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were then subjected to SDS-PAGE at a constant voltage of 100 V for 1 hour at room temperature and transferred to nitrocellulose membrane (BIO-RAD) at a constant voltage of 30 V at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight at 4 °C with the following primary antibodies (1:100, in blocking buffer): mouse anti-human MBP (BMK-13, antihuman MBP (BMK-13, MCA5751 Bio-RAD), rabbit anti-human PD-L1 (E1L3N ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was decanted and the cell pellet was washed twice with cold cPBS (PBS treated with Chelex-100 resin (Bio-Rad) for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Neuroscience 2021Quote: ... was then added and the solution was mixed at 4°C for 10 minutes before addition of 100 mg of Biobeads SM2 (Bio-Rad). Biobeads were washed into methanol ...
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bioRxiv - Molecular Biology 2021Quote: ... pH 9.0 running buffer and visualized using the FITC channel set at 100 µM resolution in low intensity mode on a GelDoc Pharos imager (BioRad Laboratories). ImageQuant software was used to analyse the fraction of probe bound in each lane and GraphPad Prism version 8 software was used to calculate the Kd ...
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bioRxiv - Molecular Biology 2020Quote: ... 100-500 ng of total RNA was reverse-transcribed into complementary DNA (cDNA) by using iScript™ Reverse Transcription Supermix (Biorad). Quantitative real-time PCR was performed using iTAQ™ SYBR Green PCR Kit (Biorad ...
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bioRxiv - Biophysics 2022Quote: ... 0.5 μg of total RNA sample (100 ng/μl) was retrotranscribed in stable cDNA with iScriptTM Advanced cDNA Synthesis Kit (Bio-Rad). Genes of interest were amplified with Itaq UniversSYBR Green (Bio-Rad) ...
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bioRxiv - Biophysics 2019Quote: ... Temperature was increased at a rate of 1°C per minute from 4°C to 100°C and fluorescence was measured every minute with a qPCR thermocycler in FRET mode (Bio-Rad). Aggregation temperature was determined by locating the global minimum of the second derivative of the raw data ...
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bioRxiv - Physiology 2019Quote: ... in TBE buffer against a 100 bp DNA ladder (GeneRuler™, Fermentas) using a Model 200/2.0 Power Supply (Bio-Rad). Gels were imaged using a Genius Bio Imaging System and GeneSnap software (Syngene).
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bioRxiv - Molecular Biology 2019Quote: ... The cDNA was generated from 100-300 ng of total RNA using iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad). cDNA was diluted 2X with water ...
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bioRxiv - Cell Biology 2019Quote: ... Intact cells were divided into 100-μl aliquots and heated individually at different temperatures for 3 minutes in a PCR machine (Thermal cycler, BIO-RAD), followed by cooling for 2 minutes at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... qPCRs were performed using 2ul of 1:100 diluted first PCRs as a template with qPCR primers (Table S4) and iTaq Universal SYBR Green Supermix (Bio-Rad) according to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... All washes were done with 100 µl PBST (PBS with 0.05% Tween 20) using a handheld magnetic washer (Bio-Rad #171020100). The incubation time on the magnet was always 2 min ...
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bioRxiv - Neuroscience 2021Quote: ... for 10 minutes followed by 15 minutes of tris-buffered saline with 0.25% Triton X-100 then blocked for 1 hour in tris-buffered saline with Tween 20 solution at 0.05% (BioRad #161-0781) supplemented with sterile 2% Normal Donkey serum (Abcam #ab138579 ...
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bioRxiv - Biochemistry 2021Quote: ... Western blot was performed using 8 or 10% mini gels followed by wet transfer (1 h, 100 V) to Western grade nitrocellulose (Bio-Rad). Blots were incubated in Intercept blocking buffer (LI-COR ...
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bioRxiv - Synthetic Biology 2021Quote: ... Sample (100 μL) was analyzed using an Agilent 1200 Series HPLC instrument equipped with an Aminex HPX-87H column (Bio-Rad). Water containing 5 mM sulphuric acid was used as the mobile phase ...
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bioRxiv - Neuroscience 2020Quote: ... and 25-100 µg of total protein was loaded onto 8 to 15 % SDS-PAGE minigels (Bio-Rad Laboratories, Hercules, CA) prior transfer onto PVDF membranes ...
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bioRxiv - Cancer Biology 2020Quote: ... Between 50 and 100 µg of total protein was loaded onto a gradient (4-15%) polyacrylamide gel (BioRad, Hercules, CA, USA). Proteins were then transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2021Quote: ... gel containing 8 M urea (48% (w/v)) and 1X TBE was prerun for 30 min at 100 V in a vertical mini- PROTEAN tank (Bio-Rad). Gels were flushed and loaded with 10 μg of total RNA in 2X TBE/Urea sample buffer (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... 100 µg of protein were loaded per lane and electrophoresed on a 10% TGX precast SDS PAGE gel from Bio-Rad. Protein was transferred onto a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2021Quote: A total of 100 prophase-I arrested oocytes were pooled and mixed with Laemmli sample buffer (Bio-Rad, cat #161-0737) and denatured at 95°C for 10 min ...
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bioRxiv - Bioengineering 2022Quote: Samples were washed in PBS and fixed in 3% paraformaldehyde (PFA) for 15 minutes at room temperature (RT) and permeabilized by 0.5% Triton x-100 (Bio-Rad, USA) for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Washed cells were embedded in 2 % Low melt agarose plugs and cells were lysed (1 hr at 37°C) with lysozyme enzyme (100 μl) using CHEF Bacterial Genomic DNA Plug Kit (Bio-Rad). DNA containing plugs were washed twice with nuclease free water then treated with Proteinase K (Qiagen ...
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bioRxiv - Microbiology 2020Quote: 100 µL of well mixed anti-FLAG M2 Affinity Gel aliquots were loaded on columns (Micro Bio-Spin Columns, Bio-Rad) and washed two times with 1 mL of cell lysis buffer by gravity flow ...
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bioRxiv - Neuroscience 2021Quote: ... All slides were subsequently blocked with 4% BSA and 1% Triton X-100 in PBS and incubated with rat anti-CD68 (1:500; Bio-Rad) and mouse-anti-GFP conjugated to Alexa Fluor 488 (1:250 ...
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bioRxiv - Cell Biology 2022Quote: ... and run at room temperature with 100 V (constant) before being transferred to polyvinylidene fluoride or nitrocellulose membranes using the Trans-Blot Turbo system (Bio-Rad) for 10 minutes at 2.5 A ...
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bioRxiv - Genetics 2019Quote: ... Oil Red O stain was extracted with 100% isopropanol and the absorbance was measured at 492 nm by a multi-well spectrophotometer (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... Binding reactions were incubated for 40 minutes at 37 °C then run on a precast 5% TBE acrylamide gel at 4 °C for 45 minutes at 100 V (Bio-Rad). After transfer to Biodyne B Modified Nylon Membrane (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... the cells were divided into 100 μl aliquots and heated individually at different temperatures for 3 minutes (Thermal cycler, BIO-RAD) followed by cooling for 2 minutes at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... Each gradient was then separated into several fractions (i.e., bottom-to-top) of equal volumes (about 100 μl) using a fraction collector (BioRad, Hercules, USA). RNA from all fractions was prepared following standard TRI Reagent® protocols (Molecular Research Center Inc. ...
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bioRxiv - Immunology 2019Quote: ... to nitrocellulose membranes using a constant voltage of 100 V for 2 h using the BioRad Midi PROTEAN Western blotting module according to the manufacturer’s instructions (BioRad, CA, USA). The membranes were blocked with blocking solution [Tris-buffered saline (TBS ...
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bioRxiv - Genetics 2021Quote: ... according to the instructions of the manufacturer or extracted using Chelex® 100 resin (Bio-Rad Laboratories Inc., Hercules, CA USA). Two final elutions of DNA were made from the kit extractions with the first being used for construction of genomic libraries for a related study and the second being used for screening of Vssc mutations after being diluted 1:5 with water ...
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bioRxiv - Neuroscience 2020Quote: ... The beads were then analyzed using flow-based Luminex™ 100 suspension array system (Bio-Plex 200; Bio-Rad Laboratories, Inc.). Standard curves were generated by Bio-plex Manager software to determine unknown sample concentration ...
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bioRxiv - Neuroscience 2020Quote: ... The bead sets were analyzed using a flow-based Luminex™ 100 suspension array system (Bio-Plex 200; Bio-Rad Laboratories). A five-parameter model was used to calculate final concentrations ...
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bioRxiv - Microbiology 2020Quote: ... whole cell lysates were incubated at 100°C for 10 minutes in 50 μl SDS loading buffer (950 μl Bio-Rad® Laemmli buffer ...
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bioRxiv - Immunology 2020Quote: ... 1:400) to identify αβT cells and mouse anti-pig SWC5 (clone b37c10, Bio-Rad AbD Serotec, Puchheim, Germany, 1:100) for identification of γδT cell subpopulation ...
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bioRxiv - Microbiology 2021Quote: ... The protein contents of 100-µl cell samples were measured using the Bio-rad Protein Assay kit (Bio-rad, CA, USA). Uptake rates were calculated from retained activity and total added activity and expressed in pmol amino acid µg-1 of protein.
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bioRxiv - Immunology 2022Quote: ... 10 mM sodium bicarbonate in iron-free HEPES-buffered saline (HEPES 20 mM, NaCl 150 mM, treated with Chelex-100 chelating resin [Bio-Rad, Hercules ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... BP176-100) and run on a 10% SDS-PAGE using the TGX Stain-free FastCast Acrylamide Kit (Bio-Rad Cat# 1610183) and the Mini-PROTEAN system (Bio-Rad Cat# 1658006) ...
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bioRxiv - Plant Biology 2022Quote: ... Infiltrated leaves were infiltrated with 100 µM D-luciferin and LUC activity was quantified after 48 h using a low-light CCD imaging apparatus (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... The pellet was resuspended in 50 μL 8M urea buffer (8 M urea, 100 mm NaHPO4, pH 8.5) and boiled in Laemmli sample buffer (BioRad, 161-0737) at 98°C for 5 min ...
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bioRxiv - Immunology 2022Quote: ... Blocking buffer was removed and 100 µL/well of samples and IFN-γ standards (recombinant bovine IFN-γ; Bio-Rad Antibodies) starting at 300 ng/mL followed by two-fold dilutions added ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were boiled at 100°C for 5 min after addition of 50 µl of 4x Laemmli sample buffer (BioRad, 1610747) containing 2-Mercaptoethanol (BME) ...
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bioRxiv - Cell Biology 2024Quote: Proteins were wet-transferred to polyvinylidene difluoride (PVDF) membranes at 100 V for 1 h using a Mini Trans-Blot Cell (Bio-Rad). Membranes were blocked with either 5% milk (CD63 detection ...
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bioRxiv - Microbiology 2024Quote: ... 80µl of ice-cold bacterial cells were mixed with 1µl plasmid (pBAD28 == pEMPTY or pHND10 == pAPY, 100-200ng, key resources table) in chilled 0.1cm cuvettes (Bio-Rad, cat# 1652089) and immediately electroporated (Voltage = 1.8kV ...