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Citations for Bio-Rad :
1001 - 1050 of 1641 citations for Siglec 5 Human HEK293 His Flag Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: ... Quantitative PCR was performed in 10 µl reaction containing 5 µl SsoAdvanced™ Universal SYBR Green Supermix (BioRad), 1 µl H20 ...
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bioRxiv - Physiology 2024Quote: ... Membranes were blocked with Tris-buffered saline with 0.05% Tween-20 (TBST) containing 5% dried nonfat milk (Biorad). Primary antibodies were diluted in TBS-T containing 5% dry nonfat milk (Biorad) ...
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bioRxiv - Cell Biology 2023Quote: ... heated to 60 LJC for 5 min and electrophoresed into a 12% Tris-HCl Criterion gel from BioRad at 50 V for 20 min or until the dye front moved just below the bottom of the sample wells ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples were boiled for 5 minutes at 95°C in 4x Laemmli Sample Buffer (Bio-Rad, 1610747) and run on SDS-acrylamide gels ...
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bioRxiv - Cancer Biology 2022Quote: ... Luminex analyses were performed using the Bio-Plex ProTM Human Cytokine 27-Plex Assay as well as the Bio-Plex Pro™ TGF-β Assay (both Bio-Rad Laboratories, Hercules, USA) according to the manufacturer’s protocols on the Bio-Plex® Reader 200 by using the Bio-Plex Manager™ software.
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bioRxiv - Cell Biology 2021Quote: Secreted IL-6 and TNFα cytokines were measured in an amnion and choriodecidua-conditioned medium using a Bio-Plex Pro™ Human Cytokine 27-plex Assay (Bio-Rad, Marnes-la-Coquette, France) as recommended by the manufacturer along with Luminex technology ...
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bioRxiv - Immunology 2022Quote: ... The levels of soluble immune mediators were measured in TA samples using a high-throughput microbeads array (Bio-Plex Pro™ Human Cytokine 27-plex Assay, Bio-Rad Laboratories, Hercules, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were boiled for 5 min and equal volumes were loaded onto a 4-15% gradient gel (Bio-Rad) for SDS-PAGE ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Each female was macerated in a 50 μl 5% Chelex (Chelex 100 Resin, Bio-Rad Laboratories, Hercules, CA, USA) solution and 1μl proteinase K ...
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bioRxiv - Developmental Biology 2021Quote: ... The PVDF membrane was blocked for 30 minutes at RT using 5% Blotting-Grade Blocker (170-6404, BIO-RAD)/Tris buffered saline with Tween 20 (TBST ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were incubated in 5% BLOT-QuickBlocker as follows: goat anti-mouse HRP conjugate (BioRad, 1706516; 1:5000), donkey anti-rabbit HRP conjugate (Amersham ...
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bioRxiv - Microbiology 2022Quote: ... NS5B-FAM probe: 5’-ATGGGTTCGCATGGTCCTAATGACACAC-3’) and the GAPDH loading control (PrimePCR Probe assay with HEX probe, Bio-Rad). Each 20 μL reaction contained 500 ng of total RNA ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μl of lysate was used for protein determination by the DC Protein Assay (Bio-Rad Laboratories, Hercules, CA).
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bioRxiv - Genomics 2022Quote: ... The gel was then soaked into the staining solution for 5 minutes and then directly scanned by Bio-rad ChemiDoc™ Touch Imaging System and the gel image was analyzed.
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bioRxiv - Developmental Biology 2020Quote: ... the membrane was blocked overnight at 4°C using 5% non-fat dry milk (Bio-Rad, catalog 170-6404) in Tris-buffered saline pH 7.3 ...
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bioRxiv - Microbiology 2020Quote: ... or by hand at 5 μl per well into 96-well hard shell low profile PCR plates (Bio-rad) for bulk RNA sequencing ...
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bioRxiv - Neuroscience 2019Quote: ... ~5 μg of protein per lane was separated on 4-15% TGX gels (Bio-Rad Laboratories, Hercules, CA, USA) at 200V for 40 minutes in tris-glycine running buffer (Bio-Rad) ...
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bioRxiv - Bioengineering 2020Quote: The cDNA was diluted 1/5 with dH2O and 2.5 µL were used for qPCR analysis (10 µL total volume; BioRad, iTaq Universal SYBR Green Supermix ...
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bioRxiv - Microbiology 2019Quote: ... and boiled for 5-10 min before being run on 10-12.5% SDS-PAGE or precast Mini-Protean TGX (Biorad) gels ...
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bioRxiv - Immunology 2019Quote: ... qPCR reactions were carried out in 10μl reaction volume with 5 μl iTaq Universal SYBR Green Supermix (Bio-rad), 2 μl Primer mix and 3 μl of each cDNA sample ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were heated at 55° C for 5 minutes and 30 µg of protein per sample was run on Tris-Tricine 16.5% precast polyacrylamide gels (BioRad) for Aβ or 10% Tris-Glycine (TGX ...
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bioRxiv - Cancer Biology 2020Quote: ... Alkaline phosphatase was developed with 5-bromo-4-chloro-3-indolyl phosphate and Nitroblue tetrazolium (BCIP/NBT; BioRad, Germany) as substrate ...
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bioRxiv - Physiology 2021Quote: ... Samples (5 μL) were then loaded on precast gradients (4-15%) SDS-polyacrylamide gels in duplicate (Bio-Rad Laboratories) and subjected to electrophoresis at 180 V for 40 minutes using pre-made 1x SDS-PAGE running buffer (Ameresco) ...
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bioRxiv - Cell Biology 2021Quote: ... Nuclear (5 µg total protein) and cytoplasmic (10 µg total protein) fractions were boiled in Laemmli buffer (Bio-Rad), run on Criterion 4-20% Gels (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... 40 µL of supernatant containing 5 µg of proteins were mixed to 36 μL Laemmli Buffer 1x (Bio-Rad) and 4 µL β-mercaptoethanol BioUltra (Sigma ...
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bioRxiv - Synthetic Biology 2021Quote: ... the membrane was placed in Pierce chemiluminescence substrates for 5 minutes and imaged on film using ChemiDoc (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed in a solution of TBS+0.01 % Tween-20 (TBS-T) and incubated in 5 % non-fat milk (Biorad) blocking solution for 2 h ...
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bioRxiv - Cell Biology 2021Quote: ... 5 to 50 µg of DNA was electroporated (375 V, 1,000 µF, 750 Ω; GenePulser Xcell; BioRad, Hercules, CA) into trophozoites ...
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bioRxiv - Cell Biology 2020Quote: ... Standard qPCR reactions with 5 ng cDNA per reaction were performed with iTaq Universal SYBR Green Supermix (Bio-Rad) in the CFX Connect Real-Time PCR Detection System ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were heated for 5’ at 95°C in Lämmli buffer and protein lysates were resolved on polyacrylamide Mini-PROTEAN TGX gels (BioRad) and transferred onto nitrocellulose membrane by wet-transfer ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR was performed on 5 ng/μL cDNA using iTaq Universal SYBR Green Supermix (Bio-rad, 64361172) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Onion epidermal cells were bombarded with 5 μg of each DNA construct using a helium biolistic gun (BIO-RAD). Treated epidermal cells were kept in the dark at room temperature for 16 h before analysis by confocal microscopy (Confocal Olympus FV 1000) ...
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bioRxiv - Microbiology 2020Quote: ... purified on Ni-NTA resin (G-Biosciences, #786-940 in 5 ml Bio-Scale™ Mini Cartridges, BioRad #7324661), then dialyzed ...
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bioRxiv - Neuroscience 2020Quote: ... and blocked with 5% (w/v) non-fat milk tris-buffered saline (TBS) with 0.1% Tween-20 (Bio-Rad) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was diluted 1/5 and analyzed by RT-qPCR using 2X iTaq Universal Sybr Green Supermix (Bio-Rad) and the oligonucleotides indicated in Table S2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... pH 8.3) running buffer by loading 5 μg sample onto 4-20% Mini-PROTEAN® TGXTM gels (Bio-Rad) with iBrightTM Prestained Protein Ladder (#LC5615 ...
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bioRxiv - Neuroscience 2019Quote: ... Neurons were biolistically transfected after 5-6 days in vitro using a Helios Gene Gun (120 psi; Bio-Rad) with pLenti-hSyn-eNpHR3.0-EYFP (eNpHR3.0 fused to EYFP and driven by the human synapsin I promoter ...
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bioRxiv - Microbiology 2019Quote: ... the infected cell lysates were incubated for 5 min at 55°C in a S1000 thermal cycler (Bio-Rad). The total volume of each reaction was 30 μl ...
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bioRxiv - Synthetic Biology 2020Quote: ... Genomic DNA was obtained from single adults via incubation in 100µL of 5% w/v Chelex beads (BioRad Inc.) at 55°C ...
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bioRxiv - Microbiology 2020Quote: ... 20 mL blocking buffer supplemented with 5 μL of horseradish peroxidase (HRP) conjugated rabbit anti-mouse secondary antibody (BioRad) was added and incubated for 2 hours at room temperature with agitation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 15μg protein in 5x Laemmli buffer with 5% b-mercaptoethanol was loaded on Mini-PROTEAN TGX Precast Gels (BioRad). Gel lanes were cut into three sections for peptide extraction ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were washed 3 times with PBS and then blocked for 1 hour at room temperature with DPBS with 0.4% Triton X-100 and 5% non-fat dry milk (BioRad). Cells were stained with primary and secondary antibodies (Table S3 ...
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bioRxiv - Cell Biology 2020Quote: Immunoprecipitated proteins were resuspended in SDS-PAGE sample buffer and heated 5 minutes at 95°C before loading onto a 7.5% acrylamide mini-Protean TGX gel (BioRad). After 7 minutes of electrophoresis at 100 V the gel was stained with Imperial Protein stain (Thermo ...
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bioRxiv - Cell Biology 2019Quote: ... Protein samples were heated at 95°C for 5 minutes and 10µL were loaded on SDS-PAGE gels (4-20%Mini-PROTEAN TGX Stain-Free, BioRad). After electrophoresis ...
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bioRxiv - Systems Biology 2020Quote: ... A total of 800 µl of cell suspension mixed with 10 µg of pDM304_Red-FL2 in a 4-mm-width cuvette was subjected to electroporation (two 5 sec separated pulses with 1.0 kV and a 1.0 msec time constant) using a MicroPulser (Bio-Rad). These cells were plated on 4–6 pieces of 90-mm plastic dishes with HL5 medium and incubated at 22°C for 18 hours under the non-selective conditions and then cultured in the presence of 10 µg/ml G418 (Wako) ...
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bioRxiv - Genomics 2021Quote: ... 1 μL diluted cDNA in a total volume of 5 μL using a CFX384 Real-Time System (Bio-Rad). For each sample we conducted three technical replicates and used their mean as the data point ...
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bioRxiv - Genomics 2021Quote: ... They were then boiled for 5 minutes at 99°C after the addition of Laemmli buffer (Cat. #1410737, Biorad). Proteins were separated by SDS-PAGE and transferred to a membrane with a Trans Blot TURBO (Biorad) ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μM of proteins and 3x of SYPRO™ Orange were pipetted into a 96-well PCR plate (BioRad, MicroAmp Fast 96-Well Reaction plate ...
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bioRxiv - Biochemistry 2021Quote: Nucleosome quality was analyzed by running the nucleosome on native PAGE on a 5% TBE gel in 0.5X TBE buffer (BioRad) that was run for 60 min at 150 volts ...
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bioRxiv - Biochemistry 2021Quote: ... was added to a final concentration of 1X to the quenched reaction and samples were loaded onto a 5% TBE gel and run for 60 min at 150 volts in 0.5X TBE Buffer (BioRad). Gels were stained with ethidium bromide and imaged on a BioRad ChemiDoc ...