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Citations for Bio-Rad :
1151 - 1200 of 1641 citations for Siglec 5 Human HEK293 His Flag Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... Protein samples were denatured at 95°C for 5 min in appropriate loading buffer and loaded into precast SDS-PAGE gels (10% polyacrylamide; Biorad). Gels were run at 40 mA for 45 min in 1x running buffer (Fisher) ...
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bioRxiv - Plant Biology 2019Quote: ... 5 μl of 10-fold diluted cDNAs was used for qRT-PCRs using a CFX96 real-time PCR machine (BioRad) with a SYBR solution (Eurogentec ...
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bioRxiv - Genetics 2020Quote: ... nduo-5 and ctb-1 with act-1 as control by iQ™ SYBR® Green Supermix (BIO-RAD, #1708880).
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bioRxiv - Cell Biology 2021Quote: Purified substrates and enzymes were centrifuged (16,000 g, 5 min, 4°C) before protein concentration was determined by Bradford assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: qPCR reactions were carried out in 25 µl total volume containing 5 ng of cDNA sample and 0.3 µM designed primers and SYBR Green Master Mix (Bio-Rad). Amplification was performed by Step One Plus (Applied Bio System ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were denatured by boiling in 1x Laemmli buffer at 95°C for 5 minutes and loaded on a 4–20% gradient gel (BioRad). PVDF membranes were used for proteins wet transfer (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... was boiled at 95⁰C for 5 minutes prior to electrophoresis on a 4-20% gradient SDS-PAGE gel (BioRad). Membrane was blocked with 5% skim milk in TBST 1x on rocker for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... Samples (10 µg protein) were denatured at 95°C for 5 min in 4x Laemmli Sample Buffer (1610747, Bio-Rad) or Novex NuPAGE LDS Sample Buffer (NP0007 ...
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bioRxiv - Physiology 2021Quote: ... membrane-enriched fraction: 5 μg per lane) were loaded onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA) – alternating between control and high CO2 treatments to avoid possible gel lane effects ...
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bioRxiv - Immunology 2020Quote: ... and membrane blocking was performed overnight at 4°C in Tris-buffered saline (TBS) containing 0.2% Tween 20 (V/V) (TBST) and 5% (W/V) Blotting-Grade Blocker (Bio-Rad). Following overnight blocking ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins in loading buffer were boiled for 5 min before loading on to 12-15% SDS-PAGE gels using the Mini-Protean III gel system (BioRad). A protein transfer apparatus (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were stripped using Restore™ Western Blot Stripping Buffer (Cat. number: 21059) following the manufacturer’s protocol and blocked with 5% Blocking-Grade Blocker solution (Bio-Rad) for 2 h at room temperature ...
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bioRxiv - Genetics 2019Quote: ... All qPCR reactions were performed in a total volume of 5 µl containing 2.5 µl of SsoAdvanced mastermix (Bio-Rad), 2 µl of forward and reverse primers (5 µM ...
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bioRxiv - Cell Biology 2021Quote: ... Mean fluorescence intensity was measured to calculate final concentration in pg/mL using Bioplex200 and Bioplex Manager 5 software (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... Samples were heated at 95°C for 5 minutes and loaded on 4-15% TGX gels (Bio-Rad, no. 4568083) in running buffer (25 mM Tris ...
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bioRxiv - Developmental Biology 2022Quote: ... membranes were incubated in a 5 ml 1:1 A:B solution of Clarity Western ECL substrate (BioRad cat. #170-5060) for 5 minutes at room temperature with rotation ...
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bioRxiv - Genetics 2022Quote: ... Approximately 43µl of the newly formed droplet solution were transferred into a ddPCR 96-well plate and then sealed at 180°C for 5 seconds in the PX1 PCR Plate Sealer (BioRad). PCR was carried out with the following conditions ...
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bioRxiv - Plant Biology 2022Quote: ... The resin was resuspended in 5 mL of extraction buffer and transferred to a gravity flow column (Biorad, Hercules, CA). After loading ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were washed (3 × 5min TBS-T and 2 × 5 min TBS) before being imaged on the ChemiDoc MP Imaging System (Biorad). Subsequently ...
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bioRxiv - Microbiology 2022Quote: The apparent KD of GST-DnaA for the dnaX-ebfC 5’ UTR DNA probe was determined by densitometry from triplicate EMSAs using ImageLab software (BioRad). Lanes were manually set to the EMSA gel ...
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bioRxiv - Synthetic Biology 2022Quote: ... The membrane was washed for 5 min with TBS-T and then imaged using a ChemiDoc XRS+System (Bio-Rad). For chemiluminescence-based detection of immunoblots ...
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bioRxiv - Synthetic Biology 2022Quote: ... the hybrid RNA/DNA origami was run on 1.5% agarose gel in TAE buffer (75 mL were poured into a 15 cm x 10 cm gel tray, Biorad) and analysed by in-gel imaging using the specific dye DFHBI-1T as described above with few modifications due to the different gel composition (agarose instead of polyacrylamide ...
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bioRxiv - Neuroscience 2022Quote: ... R primer: 5′-GTACCACCATGTACCCAGGC-3’) cDNA were amplified using the iScript RT-PCR iQ SYBR Green Supermix (BIORAD, Cat.:# 170882) in a qPCR detection system (LightCycler LC480 ...
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bioRxiv - Microbiology 2022Quote: ... Cell equivalents of ~1.5×106 cells per lane were separated by 10% SDS-PAGE along with the Precision Plus Protein Kaleidoscope standard (BioRad). The fractionated proteins were transferred to a PVDF membrane ...
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bioRxiv - Neuroscience 2022Quote: ... After resuspension in TeSR/E8 medium supplemented with 5 μM ROCK inhibitor cells were counted with a TC20 automated cell counter (Biorad) and resuspended to get a final concentration of 2 x 10^5 cells/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... PVDF membranes were blocked for 1 hr in 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T, Bio-Rad), then incubated overnight at 4°C with antibodies listed in Table S1 in 5% BSA in TBS-T ...
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bioRxiv - Microbiology 2023Quote: ... Protein preps were boiled for 5 minutes and then separated by SDS-PAGE on a 10-20% Tris-Gycine gel (BioRad). Proteins were electro-transferred from gels to nitrocellulose membranes ...
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bioRxiv - Plant Biology 2023Quote: ... membranes were washed at least three times for 15 minutes in 1x TBST solution and incubated for 5 minutes at room temperature with either Clarity Western ECL (BioRad) or Supersignal West Femto maximum sensitivity substrates (Thermo Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were washed three times for 15 minutes in 1x TBST and immunoblots were incubated for 5 minutes at room temperature with either Clarity Western ECL (BioRad) or Supersignal West Femto maximum sensitivity substrates (Thermo Scientific) ...
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bioRxiv - Synthetic Biology 2022Quote: ... PFGE was undertaken by running samples on a 1.0% agarose gel in 0.5 X TBE solution at 14 °C on a Bio-Rad clamped homogeneous electric field apparatus (CHEF-DR III, BioRad). 6 V/cm were used with 15 h switch time of 60 s followed by 15 h 120 s at 120° ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were subsequently blocked for 2 h at room temperature with 5% non-fat milk (Bio-Rad, Hercules, CA, USA) or 5% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... The prepared assay plate was exposed to temperature increases from 25 ⁰C to 95 ⁰C with 1 ⁰C increments for 5 s each using a CFX Connect Real-Time PCR Detection System (BioRad). SYBR fluorescence was detected after every temperature increase ...
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bioRxiv - Microbiology 2022Quote: ... pBAC-BoHV-4-A-revORF45HA and pBAC-BoHV-4-A-ΔORF45KanaGalK DNAs (5 μg) were electroporated in 600 μl DMEM high without serum (Biorad, Gene Pulser XCell ...
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bioRxiv - Cell Biology 2023Quote: ... The secondary antibody was washed away 5× with TBS-T and developed with an AP Conjugate Substrate Kit (Bio-Rad). Spots were quantified using a Nikon ELISPOT system and NIS-Elements AR software (v ...
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bioRxiv - Microbiology 2023Quote: ... Appropriate secondary-HRP conjugated antibodies in 5% Milk TBST were incubated for 1 hr at room temperature prior to development with clarity ECL (BioRad) developing reagents with a ChemiDoc Touch (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... After 3x 10 min washes in TBST appropriate secondary-HRP conjugated antibodies in 5% Milk TBST were incubated for 1 hr at room temperature and developed with a ChemiDoc Touch (BioRad) as described above.
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bioRxiv - Biochemistry 2023Quote: ... 7% acetic acid for 30 minutes at room temperature followed by two 5-minute washes in Mill-Q water for imaging on the ChemiDoc (BioRad). Alternatively ...
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bioRxiv - Microbiology 2023Quote: ... Blot sections were washed 5 × 5minutes in TBST and signal was detected using Clarity Western ECL Substrate (BioRad 170 5060). Images were captured on a BioRad Chemi Doc XRS+ imager.
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bioRxiv - Cell Biology 2023Quote: ... the membrane was cut lengthwise into strips to isolate bands of particular molecular weights before being washed with TBST and incubated at room temperature for 1 H in a 5% blocking buffer solution (Biorad). The western blot strips were then incubated overnight at 4 C in a solution consisting of the primary antibody diluted into blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 40 cycles of 95 °C for 5 sec and 60 °C for 30 sec using the CFX 96 real-time systems (Biorad). The 2−ΔΔCt method was used to analyze the data (Schmittgen and Livak ...
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bioRxiv - Cancer Biology 2023Quote: ... and RT-qPCR performed on the resulting cDNA (diluted 5-fold) using the iQ SYBR Green Supermix (Bio-Rad, #1708882) and following the manufactures instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed with TBS-T (3 × 5 minutes) and incubated in horseradish peroxidase (HRP)-conjugated secondary antibody (1:1000) (BioRad) in blocking solution for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was then washed three times with TBS-T for 5 min each before developing using Clarity MaxTM Western ECL Substrate (1705062; BioRad) and imaged using a BioRad ChemiDoc XRS+.
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bioRxiv - Cell Biology 2023Quote: ... The oligonucleotide substrate products were visualized using the ChemiDoc MP Imaging System to detect Cy 5 fluorescence (Bio-Rad, 1708280).
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bioRxiv - Cell Biology 2023Quote: ... A total of 15 μg siRNA (5 μg from each siRNA) was transferred to a 4-mm cuvette (Bio-Rad) and 5-10×106 DCs were added in 200 μl OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein concentrations of the supernatants were determined using DC™ Protein Assay Kit (Bio-Rad #5 000 112). Equal concentrations of protein (30 μg ...
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bioRxiv - Genetics 2023Quote: ... The beads were washed four times for 5 min with RIP buffer containing 0.5% sodium deoxycholate and boiled in 1xLaemmli Sample Buffer (Bio-Rad) for 10 min at 100°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 μl of the sample were heated at 95°C for 5 min and resuspended in 4x Laemmli sample buffer (BioRad)/50 mM DTT before SDS-PAGE.
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bioRxiv - Molecular Biology 2023Quote: ... qPCR reactions were prepared from cDNA at 1:5 dilution with SYBR Green iTaq Universal SYBR Green Supermix (Biorad, 1725120) and qPCR primers at 10 µM in a total volume of 10 µL in a ≥ 40-cycle and melt curve reaction cycler (Biorad ...
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bioRxiv - Cancer Biology 2022Quote: ... 10-15 μg of protein samples and 3-5 μL of Precision Plus Protein Dual Color Standards (BIO-RAD, #1610374) were loaded in NuPAGE 4-12% Bis-Tris protein gels (Thermo Fisher Scientific ...