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Citations for Bio-Rad :
851 - 900 of 1641 citations for Siglec 5 Human HEK293 His Flag Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... nitrocellulose membranes were blocked in 5% blotting grade blocker nonfat dry milk (Bio-Rad, Hercules, CA) in TBS (wt/vol ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked for 5 minutes with EveryBlot Blocking Buffer (12010020 Bio-Rad, Hercules, California, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... both from IDT) for 5 minutes at 95 °C in a PCR machine (PCR ThermoCycler, Biorad). Next ...
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bioRxiv - Neuroscience 2019Quote: ... The samples were then analysed in duplicate by means of Invitrogen’s Inflammation 20-Plex Human ProcartaPlex™ Panel (#EPX200-12185-901) and read on the Bio-Plex® 200 system (Bio-Rad, 2016). The data is expressed in pg⋅mL−1 ...
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bioRxiv - Synthetic Biology 2019Quote: ... Roughly 5 ng of genomic DNA was mixed with primers and probes for a human RPP30 copy number assay labeled with HEX (Bio-Rad, Hercules, CA) as an internal control ...
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bioRxiv - Genetics 2020Quote: ... and was quantified by using a QuantifilerR Human DNA Quantification Kit (Applied Biosystem, USA) on a Bio-Rad Real-time PCR System (Bio-Rad Laboratories, USA). The quantification process was performed per the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... Cell viability dye Viva Fix 498/521 (#1351115) and mouse anti-human CD138 Alexa Fluor 647 (#MCA2459A647) were obtained from Bio-Rad (Hercules, California). CD138 magnetic beads were procured from Miltenyi Biotec (Auburn ...
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bioRxiv - Cancer Biology 2023Quote: PDEC cytokine secretion was analyzed from cleared PDEC culture supernatants using Bio-Plex Pro Human Cytokine 27-plex assay kit (Bio-Rad, cat. M500KCAF0Y) and Bio-Plex 200 System (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... Cytokine levels in cell culture supernatants was determined using the Magnetic Luminex Performance Assay (Human Base Kit A; R&D Systems, coupled with the Bio-Plex 200 (Bio-Rad). The trimmed median value was used to derive the standard curve and calculate sample concentrations.
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bioRxiv - Neuroscience 2023Quote: The reference gene used in combination with the designed human PMP22 assay was the commercially established AP3B1 (dHsaCP1000001; #10031245, Bio-Rad, Basel, Switzerland) ddPCR copy number variation assay.
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bioRxiv - Immunology 2023Quote: IgE receptor cross-linking (IgECL) on HSMCs was accomplished through sensitization with 1 μg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA) for 24 hours ...
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bioRxiv - Immunology 2020Quote: Staining of 2×105 cells per well was performed with mouse anti-human CD79α (clone HM57, Bio-Rad AbD Serotec, Puchheim, Germany, 1:100) for identification of B cells and Alexa Fluor 647-conjugated rat anti-human CD3∊ (clone CD3-12 ...
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentration was assessed in cell culture supernatants 48 h post transfection by using immunoassays formatted on magnetic beads (Bio-Plex Pro Human Cytokine 27-plex Assay #M500KCAF0Y) (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s protocol via Luminex 100 technology (Luminex ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µL of each of the human plasma samples from the small cohort were diluted in 17 µL of Laemmli sample buffer (Bio-Rad; Hercules, CA, USA) followed by denaturing at 70 °C for 10 min and the resulting denatured 20 µL samples were loaded onto 12% Bis-Tris PAGE gel (GenScript ...
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bioRxiv - Cell Biology 2020Quote: ... which then heated at 90°C for 5 min before loading onto 4-20% gel (Bio-Rad). Proteins were separated using running buffer (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... and 10 μl were loaded on a Mini-PROTEAN TGX 5%–20% Tris-Glycine gel (Bio-Rad) and run in 1X Tris Glycine-SDS (KD Medical ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was extracted with 250 μL of 5% Chelex 100 Resin (Bio-Rad laboratories, Hercules CA) and 3 μL of Proteinase K (20 mg/mL ...
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bioRxiv - Immunology 2021Quote: ... Proteins were also run on a sodium dodecyl sulphate (SDS) polyacrylamide gels (5–20% gradient; Bio-Rad) to check for purity39,40 ...
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bioRxiv - Plant Biology 2019Quote: ... 5 µg of plasmid DNA were bound to 3 mg gold micro-carriers (1 µm, Bio-Rad) by adding 50 µL of 2.5 M CaCl2 and 20 µL of 0.1 M spermidine ...
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bioRxiv - Developmental Biology 2019Quote: PVDF membranes carrying proteins were incubated in the TBST buffer with 5% nonfat dry milk (BIO-RAD, Blotting-Grade Blocker ...
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bioRxiv - Microbiology 2019Quote: ... Relative fluorescence was measured at 5-minute intervals for 2 hours in a CFX96 system (Bio-Rad).
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bioRxiv - Developmental Biology 2019Quote: ... 2 μl of gene-specific primer pair and 5 μl iTaq Universal SYBR Green Supermix (Bio-Rad). The relative transcript abundance of each gene (normalized to rpl4 ...
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bioRxiv - Genetics 2020Quote: ... was added to the membrane for 5 minutes before imaging using the ChemiDoc XRS+ system (Bio-Rad) or ImageQuant 800 (AMERSHAM).
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bioRxiv - Evolutionary Biology 2020Quote: ... qPCR mixes consisted of 5 μL of Sso Advanced SYBR Green Supermix (Bio-Rad, Hercules, California, USA), 1 μL of water ...
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bioRxiv - Genomics 2021Quote: ... DNA was visualized by staining in ethidium bromide (5 µg/mL) and imaging (Gel Doc XR+; BioRad).
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bioRxiv - Neuroscience 2020Quote: ... Denatured protein lysates (5 μg) were loaded in 4–20% Criterion TGX Precast Gels (Bio-Rad, 5678095) and transferred to Immobilon-FL PVDF membrane (0.45 μm pore ...
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bioRxiv - Cancer Biology 2020Quote: ... and 2 μl cDNA (diluted 1:5) using a CFX384 real-time system qRT-PCR machine (BioRad). Primers were designed using Benchling and purchased from Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated overnight at 4°C with primary antibodies dissolved in 5% Blotting-Grade Blocker (Bio-Rad). All primary antibodies and dilutions used are listed in Supplementary Table STm.1 ...
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bioRxiv - Neuroscience 2020Quote: ... 20 to 40μg of protein were boiled at 95°C for 5 minutes in Laemmli buffer (Biorad), separated on a SDS-PAGE gel and then electrotransfered (100V ...
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bioRxiv - Microbiology 2020Quote: ... separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis on 5– 20% gels (Bio-Rad Ready Gel J), and transferred to polyvinylidene fluoride membranes (Immobilon-PSQ ...
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bioRxiv - Plant Biology 2022Quote: ... The qPCR reaction contained 5 µl of iTaq™ Universal SYBR® Green Supermix (Bio-Rad, USA), 1 µl of 5 µM forward primer ...
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bioRxiv - Biochemistry 2022Quote: ... 2 μl of diluted vRNP mixtures was loaded onto a 5% polyacrylamide native TBE gel (Bio-Rad) and run at 125 V for 80 min at 4°C ...
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bioRxiv - Genomics 2022Quote: ... Genomic DNA was extracted using 250 μL of 5% Chelex 100 Resin (Bio-Rad laboratories, Hercules CA) and 3 μL of Proteinase K (20 mg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated with primary antibody in a solution of 5% bovine serum albumin (BSA) (Bio-Rad Laboratories) in TBS-T overnight at 4 °C ...
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β-amyloid−driven synaptic depression requires PDZ protein interaction at AMPA-receptor subunit GluA3bioRxiv - Neuroscience 2021Quote: ... boiled for 5 min and loaded on a 4-15% Criterion TGX Stain-Free precast gel (BioRad). Protein samples were transferred unto a PVDF membrane (BioRad ...
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bioRxiv - Genomics 2020Quote: ... 5 μl of total RNA was reverse transcribed using the iScript Advanced cDNA Synthesis Kit (BioRad, USA) in a 10 µL volume ...
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bioRxiv - Microbiology 2021Quote: ... Each reaction consisted of 10 μl: 5 μl of iTaqTM Universal SYBR® Green Supermix (Bio-Rad), 1 μl cDNA ...
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bioRxiv - Microbiology 2022Quote: ... Images were developed by treating blots for 5 minutes with Clarity Western ECL substrate (Bio-Rad #1705060) followed by imaging on a ChemiDoc MP System (Bio-Rad #12003154) ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 mM EGTA) and protein concentrations were determined by DC protein assay kit II (Bio-Rad #5000112). Proteins were separated on house-made SDS-PAGE gels and transferred to nitrocellulose membranes (Amersham #10600003) ...
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bioRxiv - Plant Biology 2019Quote: ... 5- to 6-week-old expanded leaves of Arabidopsis plants were bombarded with 1nm gold particles (BioRad) coated with pB7WG2.0-GFP and pB7WG2.0-RFPER ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A qPCR reaction was set up in a 10 μl reaction volume (5 μl of Bio-Rad SsoFast EvaGreen Supermix ...
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bioRxiv - Cancer Biology 2019Quote: ... washed with TBST (6 × 5 min) and developed using Clarity Western ECL Substrate (Bio-Rad, California, US). Images were captured with ChemiDoc XRS+ system (Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 to 20 μg of sample were mixed with equal volume of 2x Laemmli buffer (Bio-Rad) containing β-mercaptoethanol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was extracted using 250 μL of 5% Chelex 100 Resin (Bio-Rad laboratories, Hercules CA) and 3 μL of Proteinase K (20 mg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... at 100V for 1 hour and blocked for 1 hour with 5% non-fat milk (Bio-Rad) before incubation at 4 C overnight with appropriate primary antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were blocked in 1xTBST (0.1% Tween-20) containing 5% nonfat milk (Bio-Rad, 1706404, Hercules, USA), followed by incubation with primary antibodies diluted in 1X TBST containing 5% nonfat milk ...
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bioRxiv - Biochemistry 2021Quote: ... Blots were then blocked for 1 h at 25 °C with 5% non-fat dry milk (BioRad) in TBST (50 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... Non-specific binding to the membrane was blocked with 5% Blotting-Grade Blocker (BIO-RAD, #170-6404), followed by probing with 1:2000 dilution of monoclonal anti-flag antibody (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... were pre-soaked with 10X SSC for 5 min and assembled in dot-blot apparatus (Bio-Rad) with vacuum-on ...
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bioRxiv - Microbiology 2020Quote: ... and subjected to a 12% SDS-PAGE (Bio-Rad Mini-PROTEAN® Comb, 5-well, 1.0 mm) at 200 mV for approximately 15 min ...