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Citations for Bio-Rad :
8951 - 9000 of 10000+ citations for Hepatitis E ORF2 protein mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... 30 µg of protein were diluted in Laemmli buffer and loaded in a 4 % to 15 % gradient mini-protean TGX gel (BioRad). After electrophoresis run ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were transferred to PDVF membranes using Trans-Blot Turbo Mini 0.2 µm PVDF Transfer Packs (BioRad, Cat. No. 1704156) and the BioRad Transblot system ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant was transferred to a new Eppendorf tube and protein was quantified with Quick Start Bradford 1X Dye Reagent (BioRad). Samples were added to 4X Laemmli Sample Buffer (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were cleared by centrifugation at 14,000 g for 15 min at 4°C and protein concentration was determined using the Bradford assay (Bio-Rad). Sodium dodecyl sulfate (SDS ...
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bioRxiv - Biochemistry 2023Quote: ... the total protein concentration was determined by Bradford assay using the Quick Start Bradford 1x Dye reagent (#5000205, Bio-Rad) and BSA (#23209 ...
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bioRxiv - Neuroscience 2023Quote: ... Electrophoresis was performed at 100 V for ∼60 minutes and proteins transferred to a polyvinylidene difluoride (PVDF) membrane using a Trans-Blot Turbo Transfer System (BioRad) which utilizes Trans-Blot Turbo Midi PVDF transfer packs (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... 50 to 100 ug protein was loaded in each lane and separated on 4–20% precast polyacrylamide gel (17000436, Biorad) and transferred on PVDF membrane (1704156 ...
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bioRxiv - Pathology 2023Quote: ... Equal amounts of protein extract (50 μg) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Bio-Rad, CA, USA) and transferred electrophoretically to nitrocellulose membranes ...
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bioRxiv - Physiology 2023Quote: ... Immunolabeled proteins were detected by application of enhanced chemiluminescence Western blotting reagents (Clarity Western ECL Substrate, Bio-Rad, Hercules, CA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... and equal amounts of protein were subjected to SDS-PAGE using 4-20% gradient polyacrylamide gels (Bio-Rad, Hercules, CA) and transferred to a nitrocellulose membrane ...
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bioRxiv - Plant Biology 2023Quote: ... Ten micrograms of denatured microsomal proteins were separated by SDS-PAGE using 10% polyacrylamide gel (Mini-PROTEAN TGX precast gel, Biorad) and tris/glycine buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were boiled for 8 min at 95 °C in 1X Laemmli buffer (LB) with β-Mercaptoethanol (BioRad, 1610747), resolved on 10% polyacrylamide gels or pre-cast 4-12% Bis-Tris gradient gels (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were loaded on a 12 % SDS-PAGE gel in the Mini-PROTEAN Tetra Cell (Bio-Rad, 165-8000). Protein was then transferred to a 0.2 µm nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... and samples were loaded into individual wells of a 4-20% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad, 4561094). The gel was run in a running buffer (made in house from Sodium Chloride (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were diluted 1:10 in RIPA Lysis and Extraction buffer (Thermo Fischer) supplemented with protease inhibitor cocktail (Fischer Scientific) for use in BCA protein assay (BioRad). BCA protein assay was performed in triplicate for each diluted sample following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Radio-labeled RNA-protein complexes were extracted from beads by boiling in 70 μl of SDS loading buffer (Bio-Rad), separated on a 4-12% Bis-Tris gel ...
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bioRxiv - Molecular Biology 2023Quote: ... Obtained samples were denatured in a 1xLaemmli buffer at 95°C for 10 min and proteins separated using electrophoresis on a 4-15% mini-protean-TGX-precast-gel (BioRad). Proteins were then transferred on nitrocellulose membranes by a semi-dry method using transblot turbo transfer system (BioRad) ...
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bioRxiv - Pathology 2023Quote: ... Equal quantities of protein (30 - 60 μg) were resolved on TGX-precast gels (Bio-Rad Laboratories Inc., Hercules, CA, USA) and subsequently transferred to PVDF blotting membranes (Millipore ...
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bioRxiv - Biophysics 2023Quote: ... Labeling efficiency was determined by measuring the absorbance of pyrene (χ346nm = 42,000 M-1cm-1) and protein concentration by 660 nm assay (Bio-Rad). An SDS-PAGE shift assay for isolating single-mSA bound ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then separated on 10-20% SDS-polyacrylamide gels and were visualized by staining with Coomassie blue (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... gels were briefly washed with water and proteins were transferred (25 V, 1.3 A, 7 min) onto nitrocellulose membranes (Bio-Rad) using Bio-Rad Trans Blot Turbo Transfer system ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed and protein reduced by mixing 25μl of sample to 4.75 μl Laemmelli buffer (Bio-Rad #161-0737) and 0.25ul of beta-mercaptoethanol (Bio-Rad #161-0710 ...
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bioRxiv - Immunology 2023Quote: ... 15 to 30 µg of protein sample was processed for separation through SDS PAGE on 4–20% Criterion TGX precast gradient gels (BioRad). Gel run consisted of 15 min run at 70V followed by 45 min run at 100V ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein (5 µg) were loaded into each lane and separated on 4– 20% polyacrylamide Tris glycine SDS gels (BioRad), then transferred to 0.45 µm PVDF membranes (Millipore Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were pelleted at 10,000 g for 10 min and the supernatants were used to determine the protein concentration using Bradford assay (Bio-Rad). After a preclearing step with Protein G Sepharose (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... Separated protein bands were visualized using “Stain Free Gel” application mode on ChemiDoc MP Imaging System (Bio-Rad Laboratories, Inc.). Protein gel was transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were resolved by SDS/PAGE and transferred to nitrocellulose membranes using the Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked in 5% BSA in Tris-buffered saline containing Tween 20 (TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... 10-50 μg protein was loaded on 10% acrylamide home-made or gradient (4-15% acrylamide) commercial (BIO-RAD, 4561086) Tris-Glycine-SDS gels along a protein ladder (PageRuler prestained protein ladder ...
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bioRxiv - Cancer Biology 2023Quote: ... 30-50 µg of nuclear proteins were loaded onto a 10% SDS-PAGE and transferred on nitrocellulose membrane using TransBlot apparatus (BioRad). Membranes were subsequently incubated with TBS Tween-20® 0.01% ...
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bioRxiv - Cancer Biology 2023Quote: ... SDS-PAGE resolved proteins were transferred onto a nitrocellulose membrane using the Trans-Blot Turbo™ transfer system (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Purified GST-KIN-EC-ter fusion protein from pooled gel filtration fractions was cross-linked to Affi-gel 15 beads (BioRad) in MOPS ...
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bioRxiv - Cell Biology 2023Quote: ... 20 μg of protein per sample was resolved on a NuPage 4-12% Bis-Tris polyacrylamide mini gel (Bio-Rad) and transferred to a nitrocellulose membrane in Towbin transfer buffer containing 20% MeOH at 100V for 1 hour or 25V for 16 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates (35 µg protein) were run on 10% SDS-polyacrylamide gels and transferred to a nitrocellulose membranes (Bio-Rad, 1620115). After blocking for 10 min with 5% nonfat dried milk in PBS with 0.05% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed and blocked in 1x TBST with 5% bovine serum albumin before consecutive incubation with primary antibody (see Suppl. Table 3) and horseradish peroxidase-coupled protein A (Cymed) and detection using Clarity Western ECL Substrate (BioRad).
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bioRxiv - Immunology 2023Quote: ... Bead-bound proteins were then eluted in 2x Laemmli buffer (diluted from 4x Laemmli Sample Buffer, #161-0747, Bio-Rad) with betamercaptoethanol (#21985023 ...
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bioRxiv - Immunology 2023Quote: ... eluted proteins were run on 10% polyacrylamide gels and transferred to polyvinylidene fluoride (PVDF) membranes (Trans-Blot Turbo Transfer System, BioRad). Membranes were blocked with 5% milk in Tris-buffered saline (TBS ...
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bioRxiv - Physiology 2023Quote: ... Proteins were denatured in Laemmli buffer and separated on 10% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). The membranes were blocked with blocking buffer for 1 h in TBST containing 5% BSA or 5% milk ...
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bioRxiv - Biochemistry 2023Quote: ... The concentrated protein was run on a Superdex Increase 200 (Cytivia) gel-filtration FPLC column on Biologic DuoFlow System (Biorad). The fraction corresponding to the peak corresponding to the molecular mass of the monomer protein (∼80kDa ...
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bioRxiv - Microbiology 2023Quote: ... SDS-PAGE was performed in 1X MES buffer and proteins were transferred onto 0.2 µm nitrocellulose membranes (Bio-Rad, 1620112).
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bioRxiv - Cell Biology 2023Quote: ... Purity was confirmed by loading 1.125 µg of protein on a 12-well 12% Mini-Protean TGX gel (Bio-Rad), performing SDS-PAGE electrophoresis and fixing the protein with 40% methanol ...
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bioRxiv - Cell Biology 2023Quote: ... or poly-acrylamide gels and proteins were transferred overnight at 4°C on a nitrocellulose membrane using a liquid transfer system (BioRad). Non-specific sites were blocked with 5% non-fat dry milk in PBS 0.1% Tween 20 ...
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bioRxiv - Physiology 2023Quote: ... and left ventricle of wild-type C57 Bl6 mice was incubated with PC2 antibody (10 ug, Alomone laboratories) overnight complexed to magnetic protein G beads (Biorad). Following 3 rounds of washing ...
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bioRxiv - Immunology 2023Quote: ... between 20-30 mg protein was run on 10-20% SDS–PAGE gradient gels and transferred onto 0.22 μM PVDF membranes (1620177, Bio-Rad). After air drying to return to a hydrophobic state ...
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bioRxiv - Genetics 2023Quote: ... Quantitative analysis of protein levels was carried out using a GS-800 calibrated densitometer with ImageLab software (Bio-Rad Laboratories).
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg protein was loaded per lane on Mini-PROTEAN TGX Precast Gels (Bio-Rad Laboratories, Hercules, CA, USA). Following protein separation ...
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bioRxiv - Cell Biology 2023Quote: ... The appropriate HRP-conjugated secondary antibodies were used for the chemiluminescent detection of proteins with Clarity Max Western ECL Substrate (BioRad). Membranes were scanned with a ChemiDoc imaging system (BioRad ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins on the gel were transferred to a polyvinylidene fluoride membrane using a Transblot SD semi-dry transfer system (BioRad) at 25V for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... wet blotting (at 200 mA per gel for 1 hour) was performed to transfer proteins onto 0.2 µm nitrocellulose membranes (Bio-Rad). Total protein staining was performed according to the manufacturer’s protocol (Revert™ Total Protein Stains kit ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were resolved by SDS/PAGE and transferred to nitrocellulose membranes using the Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked with 5% BSA in Tris-buffered saline containing Tween 20 (TBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured and proteins were separated by SDS-PAGE in Criterion™ TGX Stain-Free™ Gels (Bio-Rad), and transferred onto nitrocellulose membranes with the Trans-Blot® Turbo™ Blotting System (Bio-Rad) ...