Labshake search
Citations for Bio-Rad :
8751 - 8800 of 10000+ citations for Hepatitis E ORF2 protein mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... Twice the amount of lysate was loaded onto gels for use in Western blotting and proteins were transferred to pre-equilibrated polyvinylidene difluoride (PVDF) membranes (BioRad) at 20 volts for 60 minutes using the BOLT transfer system (Thermo Fisher) ...
-
bioRxiv - Cancer Biology 2021Quote: ... proteins were transferred to 0.45 μM nitrocellulose membranes using Trans-Blot® Turbo™ Transfer System Cell system (Bio-Rad). The membrane was then blocked in 5% nonfat dry milk TBS 0.05% Tween 20 and incubated with primary antibody overnight at 4°C ...
-
bioRxiv - Cancer Biology 2020Quote: ... An equal amount of total protein (30μg) were resolved on precast 4-12% SDS-PAGE gels (Biorad, Hercules, CA, USA), transferred onto nitrocellulose membranes ...
-
bioRxiv - Neuroscience 2022Quote: ... the membrane was then treated for 45 min at room temperature with the appropriated HRP-conjugated secondary antibody: Proteins were visualized using an ECL+ detection kit (Amersham) and imaged using a Chemidoc Touch Imaging system (Biorad). Densitometry was analyzed using the ImageLab software.
-
Sparcl1/Hevin drives inflammatory and neuropathic pain through astrocyte and NMDA receptor signalingbioRxiv - Neuroscience 2022Quote: ... and 30 μg of proteins were loaded for each lane and separated on SDS-PAGE gel (4–15%; Bio-Rad). After the transfer ...
-
bioRxiv - Physiology 2022Quote: ... Total protein (30 μg) per lane was separated by SDS-PAGE using 4–15% Tris-glycine gels (Bio-Rad Laboratories) and transferred to PVDF membrane (Millipore Sigma) ...
-
bioRxiv - Cell Biology 2022Quote: ... Lysates were normalized for protein concentration and subjected to SDS-PAGE separation on stain-free tris-glycine gels (Bio-Rad), cross-linked and imaged with the Chemidoc system (Bio-Rad) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Twenty micrograms of protein were separated by SDS-PAGE and transferred to polyvinylidene fluoride membranes (Immun-Blot, Bio-RAD, USA). After being blocked with 5% nonfat dry milk in Phosphate-buffered saline (PBS ...
-
bioRxiv - Neuroscience 2022Quote: ... protein samples (10-30 μg) were boiled and separated on precasted 4-20% Criterion TGX Stain-free gels (Bio-Rad) and transferred to a nitrocellulose membrane (Amersham Protran 0.2um NC ...
-
bioRxiv - Immunology 2022Quote: ... the proteins from unstained gel were transferred to a nitrocellulose blotting membrane using Trans-Blot turbo Transfer System (Bio-Rad). The membrane was blocked with 5% skim milk in Tris buffered saline (TBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... 10 or 12.5% bis-tris gels and protein was transferred to PVDF membranes using a wet transfer system (Bio-Rad). Membranes were blocked with 5% milk in TBS-T and incubated overnight with primary antibody in either milk or BSA at manufacturer recommended concentrations ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins from cell culture were extracted by scraping the culture wells in the presence of RIPA buffer (BioRad, Hercules, CA) with protease inhibitor and phosphatase inhibitor (St ...
-
bioRxiv - Pathology 2022Quote: ... with 1% Triton X-100] and the protein concentration of each lysate was determined with a colorimetric assay (Bio-Rad). After mixing with SDS-PAGE sample buffer ...
-
bioRxiv - Pathology 2022Quote: ... 1 or 10 μg of total protein were loaded and separated by SDS–PAGE on 10% mini gels (Bio-Rad), and transferred into a 0.2 μm-pore size nitrocellulose membrane (#GE10600001 ...
-
bioRxiv - Pathology 2019Quote: ... Approximately 50 μg of proteins from each sample were separated on 4–20% Mini-PROTEAN TGX precast gels (Bio-Rad) and transferred to a PVDF membrane.
-
bioRxiv - Pathology 2019Quote: ... specific protein bands on the blots were visualized by applying enhanced chemiluminescence reagent Clarity Max Western ECL Substrate (Bio-Rad) and then recorded with a LAS-4000 Mini imager (GE ...
-
bioRxiv - Immunology 2019Quote: ... Equal amount of cell lysates (10 μl) were loaded in 4–12% Mini-PrROTEAN TGX Precast protein Gels (Bio-rad) and transferred to PVDF membrane ...
-
bioRxiv - Cancer Biology 2019Quote: Equal amounts of protein were subjected to SDS-PAGE and then transferred to a PDVF membrane (Bio-Rad, Hercules, CA). Subsequently ...
-
bioRxiv - Microbiology 2019Quote: ... the remaining debris was sedimented by centrifugation and ten µL of each sample was loaded onto a 4-20% precast TGX protein gel (BioRad). Protein samples were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE ...
-
bioRxiv - Plant Biology 2019Quote: ... Immunoblotting was performed by electrophoretically transferring the proteins from SDS-PAGE gels to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA) according to the instruction book ...
-
bioRxiv - Plant Biology 2019Quote: ... Equal amounts of the eluted proteins were separated on 10% SDS-PAGE gels and blotted onto PVDF membranes (Bio-Rad). GFP-fused proteins were detected using mouse monoclonal anti-GFP antibody (1:5,000 ...
-
bioRxiv - Plant Biology 2019Quote: ... Equal amounts of the eluted proteins were separated on 10% SDS-PAGE gels and blotted onto PVDF membranes (Bio-Rad). GFP- and RFP-fused proteins were detected using mouse monoclonal anti-GFP antibody (1:5,000 ...
-
bioRxiv - Biochemistry 2020Quote: ... normalizing each signal on the corresponding total protein lane (obtained by the enhanced tryptophan fluorescence technology of stain-free gels, BioRad).
-
bioRxiv - Synthetic Biology 2019Quote: ... A total of 3 μg of protein was loaded to Mini-PROTEAN TGX Stain-Free Gels (10% gel, BIO-RAD). Proteins were transferred to standard PVDF membranes through an OWL semi-dry transfer apparatus ...
-
bioRxiv - Cell Biology 2019Quote: ... Typically 10-25 μg total protein was separated on reducing SDS-PAGE (4-15% or 4-20% gradient gels, BioRad). Proteins were transferred to nitrocellulose membranes ...
-
bioRxiv - Cell Biology 2019Quote: ... bound proteins were eluted directly in sample buffer and samples were separated on a 4-12% Criterion Bis-Tris gel (BioRad). Proteins were transferred to PVDF membrane (Immobilon-FL ...
-
bioRxiv - Developmental Biology 2019Quote: ... Homogenates were centrifuged (11,000 rpm, 10 min, 4°C) and resultant supernatant assayed using a DC Protein Assay Kit (Bio-Rad). A total of 20 μg of sample was loaded per well onto 12% SDS-PAGE gels and subsequently transferred overnight at 4°C to Immun-Blot LF PVDF membranes (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Bound proteins were washed 3 times in 500 mM KCl and eluted on Bio-spin disposable chromatography columns (Bio-Rad) with flag peptide as described in (88) ...
-
bioRxiv - Physiology 2020Quote: ... 10-25 µg of total protein was loaded in a 15-well pre-casted gradient gel (Bio-rad, 456-8086). After electrophoresis ...
-
bioRxiv - Microbiology 2020Quote: ... and proteins were separated by denaturing electrophoresis in 12% SDS-polyacrylamide gels and transferred to a nitrocellulose membrane (Bio-Rad) with a Mini-Protean Tetra Vertical Electrophoresis Cell at 100V for 1 h at 4°C ...
-
The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... either on 7-12% Tris-glycine gels or gradient NuPAGE 4-12% Bis-Tris Protein Gels (Thermo Fischer) or gradient Criterion TGX gel (4-15%) (BioRad). List of antibodies and their working dilution used in this work are reported in (Table S4) ...
-
bioRxiv - Cell Biology 2019Quote: ... SDS dye was added to a 1X concentration and lysates (equal µg of protein) were loaded onto 4-20% Tris-glycine gels (BioRad). Gels were transferred to nitrocellulose ...
-
bioRxiv - Synthetic Biology 2020Quote: ... 5 or 10 μL of the supernatant were resolved on an Any kD TGX Stain-Free protein gel (4568126, BioRad) alongside a Chameleon Duo Pre-Stained Protein Ladder (928-60000 ...
-
bioRxiv - Molecular Biology 2020Quote: ... was used at a dilution of 1:10000 for overnight at 4°C and probed for protein using ECL chemiluminescence kit (BioRad). HPLC purified protein was taken as control ...
-
bioRxiv - Physiology 2020Quote: ... The supernatant was collected following centrifugation at 8,000g for 10 min and protein concentrations were determined in triplicate using the Bradford method (Bio-Rad). Ten micrograms of protein were subjected to SDS-PAGE on 4–20% Criterion TGX Stain-Free Protein Gel (Bio-Rad ...
-
bioRxiv - Biochemistry 2020Quote: Size exclusion chromatography for MEZ was run on a S200 HiLoad 16/600 column at 0.5mL/min with protein standards from Bio-Rad to determine the oligomeric state of MEZ in solution ...
-
bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 10 min at 21,000 × g at 4°C and Bio-Rad protein assay reagent (Bio-Rad) was used to determine the protein concentrations of lysates ...
-
bioRxiv - Developmental Biology 2019Quote: ... NuPage 4-12% Bis-Tris protein gels beofre transferring into PVDF membranes using the Trans-Blot Turbo transfer system (Biorad). Prior to blocking and antibody incubation ...
-
bioRxiv - Immunology 2020Quote: ... Equal volumes of 30 μg total protein were denatured by adding Sample Loading Buffers (BIO-RAD Laboratories; Hercules, CA, USA). Denatured protein samples were loaded onto 4-20% Tris-Glycine Gel Novex WedgeWell (Invitrogen ...
-
bioRxiv - Molecular Biology 2021Quote: ... 12.5-50 μg of protein was mixed with one third volume of 4X Laemelli Sample Buffer (Bio-Rad Laboratories 1610747) and boiled at 100 °C before loading into a polyacrylamide gel alongside either the PageRuler™ or PageRuler™ Plus Prestained Protein Ladder ...
-
bioRxiv - Microbiology 2019Quote: ... Beads were boiled in 1X Laemmli Sample Buffer and proteins were analyzed by SDS-PAGE and Bio-Safe Coomassie staining (Biorad).
-
bioRxiv - Microbiology 2020Quote: Proteins were separated by SDS-PAGE and then transferred on PVDF membranes using the Trans-Blot Turbo blotting system (Biorad). Pyocins S5 and AP41 were detected using rabbit specific polyclonal affinity-purified ɑ-S5 and ɑ-AP41 antibodies ...
-
bioRxiv - Microbiology 2021Quote: ... The nitrocellulose strips were blocked in PBS with casein for 30 mins at room temperature and then probed for 1 hour with monoclonal antibodies (mAb) recognizing H3 HA (clone 36/2 (26)) or IAV M1 matrix protein (clone MCA401, BioRad). Strips were washed and incubated for 1 hour with a horse radish peroxidase (HRP)-conjugated secondary immunoglobulin (DAKO) ...
-
bioRxiv - Microbiology 2020Quote: Supernatants of DMSO-and RAP-treated 1B4int schizonts samples following egress and invasion in Albumax-free RPMI 1640 medium were subjected to SDS-PAGE on 16.5% Mini-PROTEIN® Tris-Tricine gels (BioRad). Reduced and alkylated proteins from band slices excised from the entire separation profile were in-gel digested at 37°C overnight with 100 ng trypsin (modified sequencing grade ...
-
bioRxiv - Biochemistry 2020Quote: Aliquots (250 μg total protein) of hippocampal extracts of WAR animals subjected to freeze/thaw were treated with 5 μL of dithiothreitol (Biorad) 50 μg/μL ...
-
bioRxiv - Molecular Biology 2020Quote: ... Lysates were homogenized using a handheld cell disruptor and sample concentrations were determined using a DC Lowry protein assay (BioRad). For cell lysates ...
-
bioRxiv - Cancer Biology 2020Quote: ... Membranes were incubated with secondary antibody for 1 h and proteins were revealed by ECL Prime (Amersham) using ChemiDoc Touch imager (Biorad).
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Proteins migrated for 60 minutes at 200 V and were then transferred to a polyvinylidene fluoride membrane (Bio-Rad Laboratories) for 2 hours (70 V ...
-
bioRxiv - Neuroscience 2020Quote: ... Results were normalized to the total amount of protein measured by the Bradford assay kit (Bio-Rad Laboratories, California, USA).
-
bioRxiv - Neuroscience 2020Quote: ... Data were normalized to the total amount of protein measured by the Bradford assay kit (Bio-Rad Laboratories, California, USA).