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Citations for Bio-Rad :
9051 - 9100 of 10000+ citations for Hepatitis E ORF2 protein mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Equivalent micrograms of protein from each sample were diluted using 4X Laemmli buffer (Bio-Rad, Hercules, CA, USA, Cat # 1610747) supplemented with β-mercaptoethanol (Millipore-Sigma ...
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bioRxiv - Immunology 2024Quote: ... proteins were transferred onto a nitrocellulose membrane using the Mini Trans-Blot® Cell and Criterion™ Blotter (Bio-Rad). Proteins on the membrane were stained with Ponceau S buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Protein signals were detected with Super ECL Detection Reagent (Yeasen, 36208ES60) in a ChemiDoc™ MP Imaging System (Bio-Rad). Native proteins were extracted using Column Tissue & Cell Protein Extraction Kit (Epizyme ...
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bioRxiv - Synthetic Biology 2024Quote: ... Protein purity and size were validated on an SDS-PAGE gel (Bio-Rad Mini-PROTEAN TGX and Mini-TETRA cell). Purified proteins were stored at –80°C.
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bioRxiv - Microbiology 2024Quote: ... HKU1 NTD and pA-LS NP protein expression was analyzed using SDS-PAGE and subsequent Western blot on PVDF membrane (Biorad) using StrepMAB-Classic HRP 1:3000 (2-1509-001 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were denatured in Laemli Buffer and boiled for 10 minutes then run on a 4-20% Criterion TGX precast midi protein gel (BioRad) and transferred to a PVDF membrane (Millipore Sigma) ...
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bioRxiv - Immunology 2024Quote: ... 7.5 µg of proteins were separated on polyacrylamide gel and then transferred on nitrocellulose membrane by the Trans-Blot dry system (Biorad). Membranes were blocked on milk (5% ...
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bioRxiv - Molecular Biology 2024Quote: ... Tissue lysates containing equal amounts of total protein were mixed with 4x Laemmli Sample Buffer (Bio-Rad, Hercules, CA, USA) and incubated at 96 °C for 5 min ...
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bioRxiv - Biochemistry 2024Quote: ... We loaded 10 µL of proteins at 10 µM mixed in a 3:1 ratio with 4X Laemmli Buffer Sample (Biorad) provided with 10% DTT ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were cleared at 5000g for 5min at 4°C and protein concentration was quantified by the Bradford assay (BioRad). Finally ...
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bioRxiv - Microbiology 2024Quote: ... sonicated and their protein contents were analysed by electrophoresis on TGX Stain-free pre-cast SDS-polyacrylamide gel (Bio-Rad). Proteins were then transferred on PVDF membranes (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were transferred from gels to nitrocellulose membranes for Western Blot analysis using the Turbo-Blot transfer system (Bio-Rad). Membranes were blocked using 1% w/v BLOTTO (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2020Quote: ... and incubated with an S-specific mouse monoclonal antibody 2B3E5 (provided by Dr. Thomas Moran at ISMMS) or an HN-specific mouse monoclonal antibody 8H2 (MCA2822, Biorad) diluted in PBST containing 1% bovine serum albumin (BSA) ...
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bioRxiv - Immunology 2022Quote: ... Primary antibody incubation was performed by incubating slides with diluted monoclonal mouse α-human pan-cytokeratin antibody (Bio-Rad MCA1907T) overnight at 4 °C followed by secondary antibody incubation with HRP-labeled α-mouse antibody for 30 minutes RT.
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bioRxiv - Neuroscience 2020Quote: ... Samples were prepared and analyzed using a Bio-Plex Pro Mouse Cytokine 23-Plex Assay kit (Bio-Rad, Hercules, CA). Assays were performed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... membrane was washed repeatedly with PBS-T and incubated 1 h RT with the secondary antibody in milk PBS-T (Goat anti-mouse IgG HRP conjugate (170-6516; BioRad); Goat anti-rabbit IgG HRP conjugate (170-6515 ...
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bioRxiv - Pathology 2019Quote: ... Bands corresponding to EPCR and the housekeeping protein β-actin (mouse IgG #NB600-501, Novus, at 1:40,000) were detected by the chemiluminescence method (Clarity Western ECL Bio-Rad) and finally densitometrically measured by ImageJ 1.6.0 software.
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bioRxiv - Cell Biology 2019Quote: ... The secondary antibodies used for Western blotting were goat ⍰ -mouse IgG HRP conjugate (170-6516 from Bio-Rad; 1:2000) and goat ⍰ -rabbit IgG HRP conjugate (170-6515 from Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was washed three times with T-TBS for 3 x 5 min and incubated for 1 h with 1/1000 solution of horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG antibody (1:1000; Biorad) and washed 3 times with T-TBS and 1 time with TBS ...
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bioRxiv - Neuroscience 2020Quote: ... Goat anti-mouse IgG (H+L) horseradish peroxidase-conjugated secondary antibody (1: 8000; Cat. 170-6516, Bio-Rad, Milan, Italy) was used ...
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bioRxiv - Physiology 2021Quote: ... The secondary antibodies were goat anti-mouse HRP-linked and goat anti-rabbit HRP-linked (Bio-Rad, Hercules, CA, USA) for immunoblotting ...
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated secondary antibodies were diluted 1:10000 and 1:2000 for polyclonal Goat-α-Mouse (Bio-Rad, 170-6516) and Goat-α-Rabbit HRP (Dako ...
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bioRxiv - Immunology 2021Quote: ... Greater than 90% of purity of monocytes was achieved as determined by flow cytometry using a mouse anti-bovine CD14 FITC-labelled antibody (BioRad).
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bioRxiv - Microbiology 2020Quote: ... IL-10) responses were measured using a customized Bio-Plex Pro™ Mouse Cytokine Th1/Th2 Assay kit (Biorad, USA) with the BioPlex 200 platform according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2021Quote: ... The following day the membrane was washed with PBST twice for 10 minutes and incubated with secondary antibodies diluted in the same solution (1:10000 HRP-conjugated anti-mouse or anti-rabbit antibodies (Biorad)) in the same solution at room temperature for 45 minutes ...
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bioRxiv - Genetics 2021Quote: ... Secondary antibodies used were: horseradish peroxidase-conjugated goat anti-rabbit or goat anti-mouse IgG secondary antibody (1:5000, BioRad). Blots were visualized using chemiluminescence (Clarity Max™ ECL ...
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bioRxiv - Cell Biology 2020Quote: ... Goat α-mouse secondary IgG-HRP antibody was used at a 1:5,000 dilution (BioRad Laboratories, Hercules, CA, 170-6516). Phospho-MAPK antibodies were incubated in 1X TBST (10 mM TRIS-HCl pH 8 ...
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bioRxiv - Pathology 2022Quote: ... The CD68 primary antibody incubation was performed at room temperature for 2 hours (mouse CD68, BIO-RAD, MCA1957, 1:750) and CD11b staining (BD Pharmigen ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were washed with 1% w/v milk in PBS prior to addition of a secondary goat anti-mouse alkaline phosphatase (ALP) conjugated antibody (STAR117A, BioRad) at 1:1000 dilution in 1% w/v milk in PBS ...
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bioRxiv - Biochemistry 2022Quote: ... HRP-conjugated anti-mouse was incubated for 1 h at room temperature and visualized by enhanced chemiluminescence (Clarity Substrate, Biorad) using Amersham Imager 680.
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bioRxiv - Microbiology 2020Quote: ELISA plates were coated overnight at 4°C with 0.1 µg/mL of mouse anti-human IgG (human CH2 domain with no cross-reactivity to rhesus macaque IgG; clone R10Z8E9; BioRad) and then blocked for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... was used in a 1:2,000 dilution and HRP-conjugated goat anti-mouse secondary antibody was used in a 1:3,000 dilution (172-1011, Bio-Rad). Following gDNA based PCR screening and Western blotting ...
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bioRxiv - Genetics 2022Quote: ... followed by goat anti-mouse IgG (H+L) alkaline phosphatase (AP)-conjugated (1:3000, Bio-Rad Cat# 170-6520, RRID:AB_11125348) antibody or Alexa Fluor 546 goat anti-mouse IgG (H+L ...
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bioRxiv - Microbiology 2019Quote: ... Secondary antibodies used for staining plaque or TCID50 assays were goat anti-mouse horseradish peroxidase and goat anti-rabbit horseradish peroxidase (Biorad).
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bioRxiv - Pathology 2019Quote: ... After incubation of the blots with HRP-conjugated secondary antibodies (1:3000 for goat anti-rabbit or 1:10,000 for goat anti-mouse, Bio-Rad), specific protein bands on the blots were visualized by applying enhanced chemiluminescence reagent Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... Serum was collected at day 49 and anti-M1 titers were measured by ELISA as previously described72 with a goat anti-mouse HRP-conjugated secondary antibody at 1:5000 (BioRad). Immunized and control mice were infected subcutaneously into the flank with 2×107 cfu of the AP1 (n=17 ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies fused to HRP were used for detection (Goat anti-mouse HRP 1:3000, BioRad; Goat anti-rat HRP 1:3000, Merck Millipore; Goat anti-rabbit HRP 1:3000, BioRad)
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bioRxiv - Microbiology 2021Quote: ... followed by HRP-conjugated anti-rabbit or anti-mouse immunoglobulin antibodies and chemiluminescence Clarity or Clarity max substrate (Bio-Rad). A Bio-Rad ChemiDoc imager was used.
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were further incubated in peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000) (BioRad, Hercules, CA, USA) for 1 hr at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Supernatants of lung homogenates used for cytokine analysis were stored at −80 with 1x Pierce Proteinase Inhibitor until testing using Bio-Plex Pro Mouse Cytokine Th17 Panel A with additional GM-CSF and IL-12p70 Singleplex sets on a Bio-Plex200 Platform (BioRad). Cytokine levels were normalized against total protein measured by Bradford assay.
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bioRxiv - Immunology 2021Quote: ... were added to each well and incubated with cross-absorbed anti-mouse IgG HRP polyclonal antibody (diluted 1:10,000) (STAR117P, Bio-Rad) for 2h at RT on a Multiscan FC Plate Reader (Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The following primary antibodies were used for western-blotting at 1/1,000 dilution: anti-V5 clone SV5-Pk1 (mouse, monoclonal, BioRad, #MCA1360), anti-Rpb3 clone 1Y26 (mouse ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used for immunoblotting: Talin-1 (1:5000 dilution, Mouse monoclonal, Clone 97H6; Bio-Rad, Cat# MCA4770), YAP (1:1000 dilution ...
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bioRxiv - Microbiology 2022Quote: ... Cytokines in the supernatants were then measured by using Bio-Plex Pro mouse cytokine 23-plex assay (Bio-Rad Laboratories). Likewise BALF from control and immunized mice was used to measure cytokines.
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bioRxiv - Microbiology 2022Quote: ... Membranes were rinsed 3x for 15 minutes in 1X TBST and incubated in the proper secondary antibody (Goat anti-mouse BioRad #170-6516 or Goat anti-rabbit BioRad #170-6515 ...
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bioRxiv - Neuroscience 2022Quote: ... Peripheral plasma cytokine levels were determined using Bio-Rad Pro mouse cytokine multiplex commercial kits (Bio-Rad, Montreal, QC, Canada). Also ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed three times before the addition of HRP-conjugated anti-mouse IgG (H+L; Bio-Rad; 170-6516) at 1:3,000 and anti-mouse IgG2b (ab97250 ...
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bioRxiv - Microbiology 2023Quote: ... fed nymph and mouse bladder samples using the iScript Reverse Transcription supermix kit according to the manufacturer’s instructions (Bio-Rad). Parallel cDNA reactions were carried out in the absence of reverse transcriptase ...
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bioRxiv - Molecular Biology 2023Quote: The procedure was performed according to the instructions of Bio-Plex Pro Mouse Cytokine 23-plex (Bio-Rad, CA, USA). Serum was diluted 4 times with Sample Diluent ...
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bioRxiv - Genetics 2023Quote: ... Blots were washed in 1x TBST with mild agitation before incubation with horseradish peroxidase-conjugated secondary antibodies diluted in blocking buffer (α- mouse 1:10,000 (170-6516; BioRad), α-rabbit 1:10,000 (170-6515 ...