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Citations for Bio-Rad :
8351 - 8400 of 9625 citations for Recombinant Human EGFR protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were eluted by heating the beads at 95°C for 5 min in Laemmli Sample Buffer (1610747, Bio-Rad) with 50 mM DTT followed by magnetic separation ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein samples were resolved by SDS-PAGE according to standard protocol and transferred onto 0.45μm nitrocellulose membranes (Bio-Rad 1620115) and blotted with the primary and secondary antibodies as described.
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bioRxiv - Cell Biology 2022Quote: ... 15-35 µg protein were loaded and separated by SDS-PAGE in 4–20% Mini-Protean TGX Precast gels (BioRad). Gels were transferred to PVDF membranes using the iBlot system (Thermo) ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein pellet was resuspended in 50 μL 8 M urea in 50 mM ammonium bicarbonate and the protein concentration determined using a Bradford assay (BioRad). Proteins were reduced using 10 mM dithiothreitol for 30 min at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... 750 ng of wtXLF and each tandem dimer construct were loaded and run on a 4-15% Mini-PROTEAN TGX Precast Protein Gel (BioRad). tdXLF constructs where the KBM of subunit 1 was replaced with additional linker sequence (lane 4 tdXLFΔKBM/WT and lane 6 tdXLFΔKBM/ΔKBM ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred onto a nitrocellulose membrane using Trans-Blot® Turbo™ RTA Midi Nitrocellulose Transfer Kit (Bio-Rad) in the Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... Between 3 and 10 μg of total protein were loaded on to a 4-15% gradient TGX gel (Bio-Rad) and transferred on to a PVDF membrane (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of whole protein from each sample was subjected to SDS-PAGE using the TGX Stain-Free FastCast Acrylamide kit (BioRad). Protein bands were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extracted from tobacco or kiwifruit were separated on 12% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad) using a highly efficient wet protein transfer system (eBlot L1 ...
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bioRxiv - Neuroscience 2020Quote: ... 0.02% bromphenol blue) for 15min at 50°C and 5μg of protein were separated on a 4-20% SDS PAGE (Biorad, #4561096) and blotted onto nitrocellulose membranes by wet transfer ...
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bioRxiv - Microbiology 2020Quote: ... After electrophoresis the separated proteins were electroblotted onto a PVDF membrane using a Trans-Blot Turbo Transfer System (Bio-Rad) with a standard protocol for seven minutes ...
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bioRxiv - Developmental Biology 2021Quote: Protein samples were loaded on SDS-PAGE gel and then were transferred to Nitrocellulose membrane (Bio-Rad, Cat. Nr. 1620112). After blocking for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were separated on a 10% SDS-polyacrylamide gel and then transferred to a nitrocellulose membrane (BioRad, Boston, MA, USA). The membrane was incubated with the appropriate primary antibody and then incubated with a species-appropriate secondary antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... and adjusted before isolation of protein aggregates as well as verified by running reduced samples on Criterion XT Precast Gel 4-12% Bis-Tris (BioRad) and staining with Coomassie Brilliant Blue R-250 staining solution (BioRad) ...
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bioRxiv - Biophysics 2020Quote: ACE2-hFc and CR3022 neutralizing antibody binding studies with the various RBD derivatives purified from different expression platforms were carried out using the ProteOn XPR36 Protein Interaction Array V.3.1 from Bio-Rad. Activation of the GLM sensor chip was performed by reaction with EDC and sulfo-NHS (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... The protein from the 5% of beads was eluted by boiling the beads in Laemmli sample buffer supplemented with BME (BioRad) and 2 mM biotin at 90°C for 10 min ...
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bioRxiv - Plant Biology 2021Quote: ... proteins from SDS-PAGE gels were transferred onto nitrocellulose membranes using the Transblot Turbo RTA Midi Transfer kit (BioRad, USA). Mouse anti-α-tubulin antibody (Sigma-Aldrich ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... 5 µg of protein was loaded per well on a 10% TGX 10-well 30µl/well gel (456-1033, Bio-Rad) for 1 hr ...
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bioRxiv - Biochemistry 2021Quote: ... the gels were either stained with One-Protein Blue gel stain (#21003-1L, Bioticum) or transferred onto PVDF membranes for immunodetection using TurboBlot system (Biorad).
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bioRxiv - Plant Biology 2021Quote: ... 20 μg total protein was separated on 10% SDS-PAGE gels and blotted onto Immun-Blot PVDF membrane (Bio-Rad). LSD1-GFP ...
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bioRxiv - Physiology 2021Quote: ... Proteins (50 μg) were loaded onto 12–15% SDS-PAGE gels using a Mini-Protean apparatus (Bio-Rad, CA, USA) and transferred onto nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... Lämmli buffer was added and identical protein amounts of the different samples were loaded on TGX Stain-free Precast gels purchased from Biorad. After the run the gels were activated by a 45 sec UV exposure and electroblotted onto nitrocellulose membranes ...
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bioRxiv - Physiology 2021Quote: ... Approximately 10 µg of protein was loaded into a 4% - 20% Criterion pre-cast gel (Bio-Rad, Hercules, CA, USA) and resolved at 120 V for 120 min ...
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bioRxiv - Neuroscience 2021Quote: ... We ran 40 µg of protein on 10% gels using the Mini-PROTEAN Tetra Cell western blotting system (Bio-Rad). Anti-NaV1.1 (Ab5204a ...
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bioRxiv - Microbiology 2021Quote: ... Twice the amount of lysate was loaded onto gels for use in Western blotting and proteins were transferred to pre-equilibrated polyvinylidene difluoride (PVDF) membranes (BioRad) at 20 volts for 60 minutes using the BOLT transfer system (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were transferred to 0.45 μM nitrocellulose membranes using Trans-Blot® Turbo™ Transfer System Cell system (Bio-Rad). The membrane was then blocked in 5% nonfat dry milk TBS 0.05% Tween 20 and incubated with primary antibody overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... An equal amount of total protein (30μg) were resolved on precast 4-12% SDS-PAGE gels (Biorad, Hercules, CA, USA), transferred onto nitrocellulose membranes ...
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bioRxiv - Neuroscience 2022Quote: ... the membrane was then treated for 45 min at room temperature with the appropriated HRP-conjugated secondary antibody: Proteins were visualized using an ECL+ detection kit (Amersham) and imaged using a Chemidoc Touch Imaging system (Biorad). Densitometry was analyzed using the ImageLab software.
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Sparcl1/Hevin drives inflammatory and neuropathic pain through astrocyte and NMDA receptor signalingbioRxiv - Neuroscience 2022Quote: ... and 30 μg of proteins were loaded for each lane and separated on SDS-PAGE gel (4–15%; Bio-Rad). After the transfer ...
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bioRxiv - Physiology 2022Quote: ... Total protein (30 μg) per lane was separated by SDS-PAGE using 4–15% Tris-glycine gels (Bio-Rad Laboratories) and transferred to PVDF membrane (Millipore Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were normalized for protein concentration and subjected to SDS-PAGE separation on stain-free tris-glycine gels (Bio-Rad), cross-linked and imaged with the Chemidoc system (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Twenty micrograms of protein were separated by SDS-PAGE and transferred to polyvinylidene fluoride membranes (Immun-Blot, Bio-RAD, USA). After being blocked with 5% nonfat dry milk in Phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: ... protein samples (10-30 μg) were boiled and separated on precasted 4-20% Criterion TGX Stain-free gels (Bio-Rad) and transferred to a nitrocellulose membrane (Amersham Protran 0.2um NC ...
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bioRxiv - Immunology 2022Quote: ... the proteins from unstained gel were transferred to a nitrocellulose blotting membrane using Trans-Blot turbo Transfer System (Bio-Rad). The membrane was blocked with 5% skim milk in Tris buffered saline (TBS ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 or 12.5% bis-tris gels and protein was transferred to PVDF membranes using a wet transfer system (Bio-Rad). Membranes were blocked with 5% milk in TBS-T and incubated overnight with primary antibody in either milk or BSA at manufacturer recommended concentrations ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins from cell culture were extracted by scraping the culture wells in the presence of RIPA buffer (BioRad, Hercules, CA) with protease inhibitor and phosphatase inhibitor (St ...
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bioRxiv - Pathology 2022Quote: ... with 1% Triton X-100] and the protein concentration of each lysate was determined with a colorimetric assay (Bio-Rad). After mixing with SDS-PAGE sample buffer ...
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bioRxiv - Pathology 2022Quote: ... 1 or 10 μg of total protein were loaded and separated by SDS–PAGE on 10% mini gels (Bio-Rad), and transferred into a 0.2 μm-pore size nitrocellulose membrane (#GE10600001 ...
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bioRxiv - Pathology 2019Quote: ... Approximately 50 μg of proteins from each sample were separated on 4–20% Mini-PROTEAN TGX precast gels (Bio-Rad) and transferred to a PVDF membrane.
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bioRxiv - Pathology 2019Quote: ... specific protein bands on the blots were visualized by applying enhanced chemiluminescence reagent Clarity Max Western ECL Substrate (Bio-Rad) and then recorded with a LAS-4000 Mini imager (GE ...
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bioRxiv - Immunology 2019Quote: ... Equal amount of cell lysates (10 μl) were loaded in 4–12% Mini-PrROTEAN TGX Precast protein Gels (Bio-rad) and transferred to PVDF membrane ...
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bioRxiv - Cancer Biology 2019Quote: Equal amounts of protein were subjected to SDS-PAGE and then transferred to a PDVF membrane (Bio-Rad, Hercules, CA). Subsequently ...
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bioRxiv - Microbiology 2019Quote: ... the remaining debris was sedimented by centrifugation and ten µL of each sample was loaded onto a 4-20% precast TGX protein gel (BioRad). Protein samples were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Plant Biology 2019Quote: ... Immunoblotting was performed by electrophoretically transferring the proteins from SDS-PAGE gels to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA) according to the instruction book ...
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bioRxiv - Plant Biology 2019Quote: ... Equal amounts of the eluted proteins were separated on 10% SDS-PAGE gels and blotted onto PVDF membranes (Bio-Rad). GFP-fused proteins were detected using mouse monoclonal anti-GFP antibody (1:5,000 ...
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bioRxiv - Plant Biology 2019Quote: ... Equal amounts of the eluted proteins were separated on 10% SDS-PAGE gels and blotted onto PVDF membranes (Bio-Rad). GFP- and RFP-fused proteins were detected using mouse monoclonal anti-GFP antibody (1:5,000 ...
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bioRxiv - Biochemistry 2020Quote: ... normalizing each signal on the corresponding total protein lane (obtained by the enhanced tryptophan fluorescence technology of stain-free gels, BioRad).
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bioRxiv - Synthetic Biology 2019Quote: ... A total of 3 μg of protein was loaded to Mini-PROTEAN TGX Stain-Free Gels (10% gel, BIO-RAD). Proteins were transferred to standard PVDF membranes through an OWL semi-dry transfer apparatus ...
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bioRxiv - Cell Biology 2019Quote: ... Typically 10-25 μg total protein was separated on reducing SDS-PAGE (4-15% or 4-20% gradient gels, BioRad). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Cell Biology 2019Quote: ... bound proteins were eluted directly in sample buffer and samples were separated on a 4-12% Criterion Bis-Tris gel (BioRad). Proteins were transferred to PVDF membrane (Immobilon-FL ...