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Citations for Bio-Rad :
8551 - 8600 of 9625 citations for Recombinant Human EGFR protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 20μg of total protein per fraction were collected and diluted with their respective solvents and 4X Laemmli buffer (Biorad, 1610747) to obtain aliquots of equal volume ...
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bioRxiv - Bioengineering 2024Quote: ... The proteins were incubated for one hour at 65 and 70°C in a thermocycler with heated lid (T100, BioRad) Binding to D25 and MPE8 antibodies was measured afterwards following the protocol described above.
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bioRxiv - Cancer Biology 2023Quote: ... Western blots were loaded at 2.5 to 50 ug total protein per lane in 4-20% gradient pre-cast polyacrylamide gels (#3450033, Bio-Rad). Gels were also loaded with the Dual pre-stained protein standards (#1610374 ...
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bioRxiv - Bioengineering 2023Quote: ... Protein transfer was performed on methanol-activated polyvinylidene fluoride (PVDF) membranes by Trans-Blot Turbo Transfer system (1704150; Bio-Rad). After transfer the membranes were blocked in 5% BSA in TBS with 0.1% Tween-20 for 1 hour at room temperature ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... Proteins were transferred onto poly(vinylidene fluoride) membrane using Trans-Blot Turbo Transfer System (Bio-Rad, Marnes-la-Coquette, France). After transfer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Total protein (mg/mL) was determined for each sample using Bradford assay reagent (Catalogue # 5000002, Bio-Rad, Hercules, California, USA). Liver mitochondria (0.175 mg/mL ...
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bioRxiv - Bioengineering 2024Quote: ... protein from gel was transferred to a PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-Rad, cat.: 1704150).
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bioRxiv - Molecular Biology 2023Quote: ... Separated proteins were transferred into a nitrocellulose membrane using the Trans-Blot Turbo transfer system (Bio-Rad, Hercules, CA, USA). The membrane was incubated with mouse polyclonal anti-GFP antibody (Roche ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteins of the parasite pellets were extracted using lysis buffer (1 mM DTT, 2X Laemmli buffer (Bio-rad, 161-0737) and protease inhibitor cocktail (Roche ...
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bioRxiv - Physiology 2022Quote: ... Protein lysates were combined with Laemmli buffer and separated on a 4-15% Mini-PROTEAN TGC precast gel (Bio-Rad) by electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated by SDS-PAGE then transferred to nitrocellulose membrane at 4°C (0.45 μm pore size, Bio-Rad). Membranes were blocked overnight at 4° in Intercept tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2023Quote: ... The protein concentration of crude cell extracts was measured using the Bradford method with BSA as a standard (Bio-Rad). The reaction mixture contains 8 μg total proteins and 4 μg nitrocefin (Calbiochem ...
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bioRxiv - Cell Biology 2023Quote: ... Then the proteins were transferred to a PVDF membrane using Trans-Blot Turbo Mini 0.2 µm PVDF Transfer Pack (BioRad, 1704156). The membranes were blocked in 5% non-fat milk in TBST for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were analyzed by SDS-PAGE and purities determined by densitometry of Coomassie Blue-stained gels (Image Lab Software, BioRad). Protein batches used in this work were ≥ 90% pure.
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were allowed to migrated on 4-20% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad Cat. 4561094) and then transferred on nitrocellulose membranes (Bio-Rad Cat ...
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bioRxiv - Neuroscience 2023Quote: ... Samples containing 20 µg of protein were separated on 4-20% Tris-Glycine SDS-PAGE gel (Bio-Rad, CA, USA) and transferred to PVDF membrane using Trans-Blot Turbo transfer system and transfer packs (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... The protein sample was denatured and run on 4-15% Mini-PROTEAN TGX Precast Protein Gels as per the manufacturer’s instructions (BIO-RAD). Blots were probed overnight at 4°C with antibodies recognizing GAD67 (1:1000 ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were visualized by Coomassie blue staining and ATG3 monomer and dimer were quantified using the ImageLab software (Bio-Rad) and the sample treated with 20 mM DTTred and 0 mM DTTox (Eh= -∞ ...
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bioRxiv - Plant Biology 2023Quote: ... or the proteins were transferred onto a nitrocellulose membrane using Mini Trans-Blot® cell (Bio-Rad, Hercules, CA, USA). The transfer was performed in 1x transfer buffer at 250 mA for 1 h at 4 °C under constant stirring ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cleaved proteins were incubated with Ni-NTA beads for revers his-purification and cleaved proteins were collected in the flow through and visualized on SDS-PAGE (Mini Protean Tris-Tricine Precast gels, BioRad).
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bioRxiv - Plant Biology 2023Quote: ... proteins separated in SDS-PAGE gels were transferred to a PVDF membrane using Trans-Blot Turbo Transfer System (Bio-Rad). For anti-His and anti-GFP blots ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were again washed three times with TTBS buffer and protein bands were visualized using the ChemiDoc Imaging System (BioRad) and quantified with Image Lab software (BioRad).
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bioRxiv - Cell Biology 2023Quote: ... proteins were separated by SDS-PAGE and transferred onto nitrocellulose membrane using a Trans-blot Turbo system (Bio-Rad, 1704159). All Western blots were revealed using ECL (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and protein concentrations were equalized prior to boiling in the recommended sample buffers supplemented with 2.5% beta-mercaptoethanol (Bio-Rad). SDS-PAGE electrophoresis was performed using 4–12% gradient bis-tris gels (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... 5uL of protein sample was loaded onto a 4-15% Mini-PROTEAN TGX Precast polyacrylamide gel from Bio-Rad (4561086). Protein samples were resolved and transferred to a PVDF membrane using the Trans-Blot STurbo Transfer System ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were spun down and the supernatant was used to measure protein concentration by using a Bradford Assay (BioRad). Equal protein concentrations were loaded onto a NuPAGE 4-12% Bis-Tris gels (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were stained with Ponceau S and total proteins were visualized using the ChemiDoc™ MP imaging system (Bio-Rad). To detect proteins of interest ...
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bioRxiv - Molecular Biology 2023Quote: ... Obtained samples were denatured in a 1xLaemmli buffer at 95°C for 10 min and proteins separated using electrophoresis on a 4-15% mini-protean-TGX-precast-gel (BioRad). Proteins were then transferred on nitrocellulose membranes by a semi-dry method using transblot turbo transfer system (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were loaded on a 12 % SDS-PAGE gel in the Mini-PROTEAN Tetra Cell (Bio-Rad, 165-8000). Protein was then transferred to a 0.2 µm nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... and samples were loaded into individual wells of a 4-20% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad, 4561094). The gel was run in a running buffer (made in house from Sodium Chloride (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were diluted 1:10 in RIPA Lysis and Extraction buffer (Thermo Fischer) supplemented with protease inhibitor cocktail (Fischer Scientific) for use in BCA protein assay (BioRad). BCA protein assay was performed in triplicate for each diluted sample following manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... Labeling efficiency was determined by measuring the absorbance of pyrene (χ346nm = 42,000 M-1cm-1) and protein concentration by 660 nm assay (Bio-Rad). An SDS-PAGE shift assay for isolating single-mSA bound ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed and protein reduced by mixing 25μl of sample to 4.75 μl Laemmelli buffer (Bio-Rad #161-0737) and 0.25ul of beta-mercaptoethanol (Bio-Rad #161-0710 ...
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bioRxiv - Immunology 2023Quote: ... 15 to 30 µg of protein sample was processed for separation through SDS PAGE on 4–20% Criterion TGX precast gradient gels (BioRad). Gel run consisted of 15 min run at 70V followed by 45 min run at 100V ...
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bioRxiv - Neuroscience 2023Quote: ... 15 μg of total protein per sample was loaded into separate lanes on a precast 10–20% electrophoresis gel (Tris-HCl polyacrylamide, BioRad). The blotRig software helps counterbalance sample positions across the gel by treatment condition. ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then separated on 10-20% SDS-polyacrylamide gels and were visualized by staining with Coomassie blue (Bio-Rad).
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bioRxiv - Pathology 2023Quote: ... Equal amounts of protein extract (50 μg) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Bio-Rad, CA, USA) and transferred electrophoretically to nitrocellulose membranes ...
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bioRxiv - Physiology 2023Quote: ... Immunolabeled proteins were detected by application of enhanced chemiluminescence Western blotting reagents (Clarity Western ECL Substrate, Bio-Rad, Hercules, CA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Ten micrograms of denatured microsomal proteins were separated by SDS-PAGE using 10% polyacrylamide gel (Mini-PROTEAN TGX precast gel, Biorad) and tris/glycine buffer ...
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bioRxiv - Physiology 2023Quote: ... and equal amounts of protein were subjected to SDS-PAGE using 4-20% gradient polyacrylamide gels (Bio-Rad, Hercules, CA) and transferred to a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were boiled for 8 min at 95 °C in 1X Laemmli buffer (LB) with β-Mercaptoethanol (BioRad, 1610747), resolved on 10% polyacrylamide gels or pre-cast 4-12% Bis-Tris gradient gels (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... the total protein concentration was determined by Bradford assay using the Quick Start Bradford 1x Dye reagent (#5000205, Bio-Rad) and BSA (#23209 ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant was transferred to a new Eppendorf tube and protein was quantified with Quick Start Bradford 1X Dye Reagent (BioRad). Samples were added to 4X Laemmli Sample Buffer (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were cleared by centrifugation at 14,000 g for 15 min at 4°C and protein concentration was determined using the Bradford assay (Bio-Rad). Sodium dodecyl sulfate (SDS ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were transferred to PDVF membranes using Trans-Blot Turbo Mini 0.2 µm PVDF Transfer Packs (BioRad, Cat. No. 1704156) and the BioRad Transblot system ...
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bioRxiv - Neuroscience 2023Quote: ... 50 to 100 ug protein was loaded in each lane and separated on 4–20% precast polyacrylamide gel (17000436, Biorad) and transferred on PVDF membrane (1704156 ...
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bioRxiv - Neuroscience 2023Quote: ... Electrophoresis was performed at 100 V for ∼60 minutes and proteins transferred to a polyvinylidene difluoride (PVDF) membrane using a Trans-Blot Turbo Transfer System (BioRad) which utilizes Trans-Blot Turbo Midi PVDF transfer packs (BioRad ...
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bioRxiv - Genetics 2023Quote: ... 30 µg of protein were diluted in Laemmli buffer and loaded in a 4 % to 15 % gradient mini-protean TGX gel (BioRad). After electrophoresis run ...
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bioRxiv - Pathology 2023Quote: ... Equal quantities of protein (30 - 60 μg) were resolved on TGX-precast gels (Bio-Rad Laboratories Inc., Hercules, CA, USA) and subsequently transferred to PVDF blotting membranes (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... gels were briefly washed with water and proteins were transferred (25 V, 1.3 A, 7 min) onto nitrocellulose membranes (Bio-Rad) using Bio-Rad Trans Blot Turbo Transfer system ...