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Citations for Bio-Rad :
8201 - 8250 of 9625 citations for Recombinant Human EGFR protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 20-40 ug of protein was loaded per lane in a pre-cast gel for SDS-PAGE (Bio-Rad). Gel was then transferred using the TurboBlot Transfer (Bio-Rad ...
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bioRxiv - Synthetic Biology 2024Quote: ... Samples prepared with equal amounts of proteins were run on 10% Mini-PROTEAN TGX Precast Gels (Bio-Rad, 4561033) (for ASGR1 blot ...
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bioRxiv - Microbiology 2024Quote: ... and total protein was wet transferred to a nitrocellulose membrane using a mini Trans-Blot electrophoretic system (Bio-Rad). After the transfer ...
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bioRxiv - Immunology 2024Quote: ... The SEC-eluted protein peaks corresponding to the correct retention volume based on the molecular weight standards (BioRad: 1511901) were collected and concentrated using Amicon Ultra centrifugal filters (Millipore Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Protein samples were loaded to a 48-well Bio-Dot® microfiltration apparatus (Bio-Rad Laboratories, Hercules, CA, USA) with BioTraceTM NT nitrocellulose membrane (Pall Life Sciences ...
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bioRxiv - Biophysics 2024Quote: ... Equal volumes of protein samples were resolved on 4-12% gradient SDS-polyacrylamide gel and transferred onto 0.2μM nitrocellulose (BioRad #1620112) or PVDF (BioRad #1620177 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were separated on a 12% SDS-PAGE gel and transferred to PVDF membranes by wet electroblotting (Bio-Rad). To improve signal detection ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were separated on a 4 to 20% Trist-glycine stain free polyacrylamide 10-well gel (Bio-Rad) at 170 V for approximately 1 h in 1x Tris/glycine/SDS running buffer ...
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bioRxiv - Microbiology 2024Quote: Protein fractions were analyzed by standard denaturing SDS-PAGE electrophoresis using 4 to 20% gradient mini-gels (Bio-Rad), InstantBlue Coomassie protein stain ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of proteins were resolved by polyacrylamide-SDS gel electrophoresis (PAGE) and transferred to nitrocellulose membrane (Bio-Rad) using Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were transferred onto a polyvinyldiene fluoride (PVDF) membrane in a Mini Trans-Blot cell (Bio-Rad, CA, USA) at a constant current of 315 mA on ice for 90 min ...
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bioRxiv - Biochemistry 2024Quote: ... separated by SDS-PAGE on a pre-cast 4–15% Criterion™ Protein Gel (Bio-Rad Laboratories, Cat#5671084), and the gel was stained using QC Colloidal Coomassie Stain (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... and at 100-120 V through the resolving gel until the protein xtra prestained ladder (Bio-Rad catalog # 1610377) achieved good separation ...
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bioRxiv - Plant Biology 2024Quote: ... Protein was transferred to 0.45 µm nitrocellulose membrane (Cytiva Amersham Protran #10600003) using a tank system (Biorad #1703930, #1704070) in transfer buffer (25 mM Tris ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were transferred to PVDF membranes with NuPAGE Transfer Buffer in a Trans-Blot Turbo transfer apparatus (Bio-Rad) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were transferred onto PVDF membranes using the Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad, 170-3930) at 80 V ...
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bioRxiv - Cell Biology 2024Quote: ... Protein was then separated by size-exclusion chromatography (HiLoad 16/600 Superdex 200 pg, Cytiva) via FPLC (Bio-Rad). Purified protein was stored in Gel Filtration Buffer + 25% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... Ribosome pellet was resuspended in same lysis buffer and protein concentration was determined via Bradford Assay (Bio-Rad, #5000205) prior to western blot.
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bioRxiv - Microbiology 2024Quote: Proteins were transferred to a nitrocellulose membrane using the Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA) and then blocked with 5% (w/v ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... were used to reveal proteins transferred on nitrocellulose membrane with the Trans-Blot Turbo Transfer System (Bio-Rad, 1704150). Membranes were blocked in PBS 0.1% Tween20 (PBST ...
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bioRxiv - Molecular Biology 2024Quote: ... The separated proteins were electrotransferred using a Trans-Blot® Turbo™ Mini PVDF Transfer Pack and System (Biorad). After blocking for 1 h in 0.5% Tween-PBS in 5% skim milk (blocking solution) ...
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bioRxiv - Microbiology 2024Quote: ... the proteins were transferred to PVDF membranes using Trans-Blot Turbo PVDF Transfer Packs (Bio-Rad, Hercules, CA, USA), and the membranes were blocked with Blocking One (Nacalai Tesque) ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate were separated on a 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (BIO-RAD), transferred to polyvinylidene difluoride membranes (0.2 um ...
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bioRxiv - Neuroscience 2021Quote: ... Each sample was prepared for western blotting by diluting 20 μg of protein with 1 × Laemmli buffer (Bio-Rad #1610747) and ultrapure water (Invitrogen #10977015 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins and peptides bearing C-terminal 6xHis tags were detected with mouse anti-6xHis antibody clone AD1.1.10 (BioRad; Cat # MCA1396GA) while detection of glycosylated CRM197-FtO-PS was with anti-F ...
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bioRxiv - Biophysics 2021Quote: ... protein was further purified by a Hydroxyapatite column (HA) connected to an NGC Medium-Pressure Liquid Chromatography System (BIO-RAD). A gradient form 5 mM NaPi ...
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bioRxiv - Biochemistry 2021Quote: ... Whole cell lysate samples containing 4 μg of protein were fractionated using a 4–20% SDS-PAGE gel (Bio-Rad). Proteins were transferred to a 0.2 μm nitrocellulose membrane using a Trans-Blot Turbo transfer system (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... 50 μg protein from each sample were resolved in 10% SDS-polyacrylamide gel and transferred onto 0.2 μm nitrocellulose membranes (Bio-Rad). The nitrocellulose membranes were blocked in 5% nonfat dry milk in Tris-buffered saline containing 0.1% Tween 20 ...
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bioRxiv - Biophysics 2020Quote: ... proteins from the supernatant were separated by 12% SDS-PAGE and transferred onto a PVDF membrane (BioRad, Hercules, CA, USA). The Signal Enhancer HIKARI (Nacalai Tesque ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of protein (5–20 μg) were loaded into each lane and separated on 4–20% polyacrylamide tris glycine SDS gels (BioRad), then transferred to polyvinylideneifluoride membranes (BioRad) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred to polyvinylidene difluoride (PVDF) membranes and chemiluminescent signal was acquired with a Chemidoc Imaging System (Bio-Rad). Images were processed with the ImageLab software (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... and protein concentrations were equalized prior to boiling in the recommended sample buffers supplemented with 2.5% beta-mercaptoethanol (Bio-Rad). SDS-PAGE electrophoresis was performed using 4-12% gradient bis-tris gels or 16% tricine gels (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: Heat-induced protein denaturation curves were obtained using a CFX Connect Real-Time PCR system (Bio-Rad Laboratories, Hercules, CA) as described previously 99 ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were run on 7.5% Mini-PROTEAN gels for cytoplasmic proteins and 12.5% Mini-PROTEAN gels for histones (Bio-Rad, #4568025). Primary antibodies used ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were desalted into phosphate-buffered saline (PBS) pH 7.4 using Econo-Pac 10DG gravity flow chromatography columns (Bio-Rad). Absorbance ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were prepared at 25 µg protein/well with 2X laemmli loading buffer and 2-mercaptoethanol and run on pre-cast 4-15% gels (Biorad). Proteins were wet transferred onto PVDF membranes ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were denatured and proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on Mini-Protean 10% tris-glycine gels (BioRad). Precision plus protein Kaleidoscope molecular weight (MW ...
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bioRxiv - Cell Biology 2020Quote: ... 10 µg of total protein lysate was loaded on to an TGX Stain Free FastCast Acrylamide gel (BioRad, cat# 1610185) and transferred using an iBlot2 gel transfer device (Thermofisher scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Lyophilized proteins were dissolved in SDS-PAGE sample buffer and resolved by SDS-PAGE (BioRad precast 4-15 % gradient gel). The gel was stained with Simple Blue Safe Stain (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... The protein extracts were resolved on 4-15% Criterion™ Tris-HCl precast gels (#3450029, Bio-Rad Laboratories, CA, USA) and blotted onto PVDF membranes (#1704157 ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared at 12 000 rpm for 10 min at 4°C and protein concentration adjusted with Bradford assay (BioRad). The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amount of proteins was loaded onto 12% SDS-PAGE gel (acrylamide: bisacrylamide 29:1, cat# 161-0146, Bio-Rad) and after size separation blotted onto 0.2 μm nitrocellulose membrane (cat# 162-0112 ...
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bioRxiv - Genetics 2021Quote: ... Proteins (7 μl of extract) were separated on Laemmli SDS-PAGE gels and transferred to nitrocellulose membranes (Bio-Rad, 1620112). Membranes were stained with Ponceau-S for visualization ...
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bioRxiv - Genetics 2021Quote: ... and 10 µg protein were resolved by SDS-polyacrylamide gel electrophoresis and transferred to a 0.2 µm nitrocellulose membrane (Bio-Rad). The membrane was blocked in 5% skimmed milk powder in PBX (0.01% Triton X-100 in PBS ...
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bioRxiv - Genetics 2021Quote: ... Proteins were visualized using horseradish peroxidase-conjugated goat anti-rabbit secondary antibody conjugate (1:2000 dilution, Bio-Rad laboratories, #1706515) or horseradish peroxidase-conjugated goat anti-mouse secondary antibody conjugate (1:2000 dilution ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were prepared for mass spectrometry by disrupting total or immunopurified ribosomes in 1X Laemmli buffer and running on a 4-20% Mini-PROTEAN TGX Precast Protein Gel in 1X Tris/Glycine/SDS buffer (BioRad) for 5 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... 0.1 OD equivalents of the resulting sample was subjected to SDS-PAGE and the proteins were separated on 4-15% Mini-PROTEAN-TGX strain-free gels (BioRad). For subsequent immuno-blotting ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 μg of total protein extracts were run on a gradient (15-4%) Polyacrylamide gel (MINIi-PROTEAN TGX Precast Gels, Biorad). Proteins were transferred on nitro-cellulose membrane and checked with Red Ponceau staining for efficient transfer ...
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bioRxiv - Molecular Biology 2021Quote: ... Denatured protein samples were then resolved in a 10% SDS-PAGE and transferred to a PVDF membrane (Bio-Rad, #1620177). Blots were probed by custom-designed Drosophila antibodies as previously described 6 diluted in PBS-0.1% Tween supplemented with 5% nonfat milk ...
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bioRxiv - Pathology 2022Quote: ... Appropriate total proteins from different lines were resolved on 10 % polyacrylamide gels and transferred to a nitrocellulose membrane (Bio-Rad). Anti-FLAG monoclonal antibody (1:1000 ...