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Citations for Bio-Rad :
8251 - 8300 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The samples adjusted to contain equal amounts of protein (10 μg or 12 μg) were next denatured (5 min at 95°C) in loading buffer (#1610747, BioRad) and run on 10% TGX (#4561034 ...
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bioRxiv - Biochemistry 2024Quote: ... the absorbance of standards and protein samples was measured at 490 nm using a microplate absorbance reader (Bio-Rad iMarkTM). See Fig ...
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bioRxiv - Physiology 2024Quote: ... Equivalent amounts of total protein were separated by SDS-PAGE on 4% to 15% Criterion TGX gels (Bio-Rad; 5671083). Megalin and cubilin were detected with anti-megalin antibody generously provided by Dr ...
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bioRxiv - Physiology 2024Quote: ... Protein extracts were denatured at 95 °C for 10 min and separated in 10% SDS-PAGE gels (1610156, Bio-Rad) at 80 V for ∼45 min followed by 110 V for ∼65 min ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred to PVDF membranes using a Trans-Blot Turbo RTA Mini 0.2 µm PVDF Transfer Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 30 μg of total protein lysate was loaded for each sample onto Mini-PROTEAN® Precast Gels (Bio-Rad, 4568123) with 4 × Laemmli buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated by SDS-PAGE electrophoresis and transferred to PVDF membranes using Trans-blot turbo transfer system (Bio-Rad). Membranes were incubated in 5% BSA in tris-buffered saline (TBS)-Tween 0.1% (TBST ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were separated by SDS-PAGE (30-50 μg total protein per lane) and transferred onto a 0.2 μm nitrocellulose membrane (Bio-Rad). Membranes were blocked with 5% skimmed milk for 30 min at RT and treated with primary antibodies (Supplementary Table S1 ...
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bioRxiv - Biophysics 2023Quote: ... Protein-DNA complexes were transferred from the gel to the PVDF membrane (Immun-Blot® PVDF Membrane, BIO-RAD, USA) using semi-dry blotting in 1x transfer buffer (48 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... protein signals were visualized by incubation of the membrane in ECL substrate (Clarity™ Western ECL Substrate, BIO-RAD, USA), and chemiluminescence was detected with Odyssey® Fc Imaging System (Li-COR ...
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bioRxiv - Biophysics 2023Quote: A total of 250 ng of purified proteins as labelled above each lane were loaded on TGX stain-free gels (BioRad) and run at constant 150V ...
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bioRxiv - Bioengineering 2023Quote: ... Protein transfer was performed on methanol-activated polyvinylidene fluoride (PVDF) membranes by Trans-Blot Turbo Transfer system (1704150; Bio-Rad). Post transfer the membranes were blocked in 5% Bovine Serum Albumin (BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... The column was washed with 50 mL of wash buffer and then transferred onto a Fast protein liquid chromatography system (BioRad). The column was further washed until the UV trace was flat ...
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bioRxiv - Cell Biology 2023Quote: ... Detection of proteins was achieved following 5 min incubation with Enhanced Chemiluminescence (ECL) reagent and exposed using a ChemiDoc imaging system (BioRad). Loading control was performed by normalizing the target protein to the stain-free images of total protein ...
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bioRxiv - Microbiology 2023Quote: ... proteins were resolved by SDS-PAGE and transferred onto a PVDF membrane by using a transfer apparatus (Bio-Rad, USA). The membrane was probed with primary antibody against V2c (1:4000) ...
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bioRxiv - Cell Biology 2023Quote: ... and equal amounts of protein were loaded and run on 4-20% gradient SDS-PAGE gels (4561096; Bio-Rad Laboratories). Proteins were transferred from SDS-PAGE gels to nitrocellulose membranes using Trans-Blot Turbo (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2023Quote: ... followed by immunoprecipitation with 5 µg appropriate antibodies or corresponding IgG control using magnetic protein A/G beads (BIO-RAD). After several rounds of washing with low-salt wash buffer (20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2023Quote: ... either on 7–12% Tris-glycine gels or gradient NuPAGE 4–12% Bis-Tris Protein Gels (Thermo Fischer) or gradient Criterion TGX gel (4–15%) (BioRad). A list of antibodies and their working dilution used in this work are reported in (Table S3) ...
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bioRxiv - Biophysics 2023Quote: ... proteins were transferred to PVDF membranes using a Trans-Blot Turbo RTA Mini 0.2 μm PVDF Transfer Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... protein from gel was transferred to a PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-Rad, cat.: 1704150).
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bioRxiv - Cancer Biology 2023Quote: ... 25 μg protein extracts were separated by sodium dodecyl sulfate (SDS) gel electrophoresis and transferred to a polyvinylidene difluoride (PVDF) membrane (BioRad) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Protein extracts were mixed with Laemmli sample buffer and separated by SDS-PAGE using 4-20% TGX gels (Bio-Rad) and transferred to 0.2 µm nitrocellulose membranes (Bio-Rad ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... Proteins were transferred onto poly(vinylidene fluoride) membrane using Trans-Blot Turbo Transfer System (Bio-Rad, Marnes-la-Coquette, France). After transfer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Total protein (mg/mL) was determined for each sample using Bradford assay reagent (Catalogue # 5000002, Bio-Rad, Hercules, California, USA). Liver mitochondria (0.175 mg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... Protein transfer was performed on methanol-activated polyvinylidene fluoride (PVDF) membranes by Trans-Blot Turbo Transfer system (1704150; Bio-Rad). After transfer the membranes were blocked in 5% BSA in TBS with 0.1% Tween-20 for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Western blots were loaded at 2.5 to 50 ug total protein per lane in 4-20% gradient pre-cast polyacrylamide gels (#3450033, Bio-Rad). Gels were also loaded with the Dual pre-stained protein standards (#1610374 ...
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bioRxiv - Immunology 2024Quote: ... Cell lysates were separated by SDS-PAGE 4 - 12 % gradient Bis-tris gel (Novex) followed by protein transfer to a PVDF membrane (BioRad) and antibody incubation ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were transferred from gels to the PVDF membrane using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad). Membranes were incubated in 5% nonfat dried milk in 1X TBST (Tris-buffered saline ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µg of protein was boiled for 5 min at 95°C and then loaded on a 4-15% SDS gel (Biorad).
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bioRxiv - Cell Biology 2024Quote: ... and the probed proteins were transferred to Bio-Rad polyvinylidene fluoride membranes using a semidry electroblotting transfer system (Bio-Rad) at 25 V for 2 h and 30 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... 70µg of protein was loaded per well for polyacrylamide gel electrophoresis using Mini-PROTEAN Precast Polyacrylamide Gels (Bio-Rad, 4561095). Gel was transferred to a low fluorescence PVDF membrane (Cytiva ...
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bioRxiv - Neuroscience 2024Quote: ... and 10 µg of protein was separated on a 4–20% sodium dodecyl sulfate polyacrylamide gel electrophoresis (Bio-Rad, #4561096) and blotted onto nitrocellulose membranes by wet transfer ...
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bioRxiv - Bioengineering 2024Quote: All relative protein quantities from western blotting were calculated by performing densitometry using ImageJ or Image Lab software (Bio-Rad) and normalizing to the average of the replicates of the PrgIWT samples ...
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bioRxiv - Molecular Biology 2024Quote: HepG2 protein lysate was separated by SDS-Page (TGX Stain-FreeTM FastCastTM Acrylamide Kit, Bio-Rad Laboratories, Inc., Hercules, US) and blotted on nitrocellulose membranes (Trans-Blot® TurboTM ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were loaded onto 10% bis-Tris gels and transferred to Immun-Blot PVDF membranes (1620177, Bio-Rad, Hercules, CA), which were then blocked in 5% blocking grade skim milk (170–6404 ...
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bioRxiv - Developmental Biology 2024Quote: ... of total protein in Laemmli Buffer were subjected to electrophoresis on precast polyacrylamide gels and transferred to PVDF membranes (BioRad). Membranes were blocked and probed overnight with primary antibodies ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred to a nitrocellulose membrane with a Trans-Blot®Turbo™ Transfer Starter System (BioRad, 170-4155). For high molecular weight samples (SYNJ1-BirA* ...
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bioRxiv - Cell Biology 2024Quote: Twenty μg of protein was loaded in each well of a Mini-protean TGX gel (Bio-Rad 4561083; Hercules, CA) with Precision Plus Protein Dual Color Standards (Bio-Rad 1610374 ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were loaded onto either a 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gel (4568094, Bio-Rad) or 4– 20% Mini-PROTEAN® TGX™ Precast Protein Gel (4561094 ...
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bioRxiv - Neuroscience 2024Quote: ... Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well (ThemoFisher, NP0321BOX) and transferred to polyvinylidene fluoride (PVDF) membranes (Biorad, 1704156EDU). PVDF membranes were blocked with 5% nonfat dried milk in Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2024Quote: ... protein bands (IP or TCE) were transferred from polyacrylamide gel to a PVDF membrane using a TransBlot Turbo device (BioRad). PVDF membrane was blocked for 10 min with 8% blocking milk in Tris-buffered saline ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting supernatant was transferred to a clean tube and protein concentration was determined using a Bradford assay (Bio-Rad). 200 μg tissue homogenate or 10 μg cell lysate was diluted in 50 μl CB lysis buffer and was used for the reaction with CatB Substrate (RR-amino-4-trifluoromethyl coumarin (AFC)).
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bioRxiv - Neuroscience 2024Quote: ... 80-100 μg of protein were loaded onto 4–20% Mini-PROTEAN® TGX™ Precast Gel (Biorad, 4561093EDU, USA) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were denatured in Laemmli loading buffer as previously described,30 25 µg of protein was separated by 7.5% SDS-polyacrylamide acrylamide gel electrophoresis (SDS-PAGE) (Criterion, Bio-Rad) and blotted onto nitrocellulose membranes ...
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bioRxiv - Neuroscience 2024Quote: ... and the separated proteins were transferred onto PVDF membranes using a Trans-Blot Turbo semi-dry transfer system (Bio-Rad). Membranes were blocked using 3% BSA in TBS with 0.1% Tween20 (TBST) ...
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bioRxiv - Biochemistry 2024Quote: ... lysates were then spun down at 14,000 rpm for 10 mn before determining protein concentration using Bradford reagent (Bio-Rad).
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated by gel electrophoresis and transferred to nitrocellulose membranes using a semi-dry blotting system (Bio-Rad Laboratories). Membranes were blocked with 3% milk or 5 % BSA in TBST for 1 h at RT ...
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bioRxiv - Biochemistry 2024Quote: ... Cholesterol values were calculated using known cholesterol solutions and normalized to protein content as measured by DC assay (Bio-Rad).
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bioRxiv - Biochemistry 2024Quote: Polar flagellins purified from bacterial growths of each mutant strain (2 µg) were separated by 12% SDS-PAGE as described previously [31] and stained with Coomassie Blue total protein stain (BioRad), according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... Thermo #26616) were run in pre-cast 4– 20% TGX stain-free denaturing protein gels (Biorad #4568093, #4568096, #5678094, #5678095) in a minigel system or criterion system (Biorad #1658005 ...