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Citations for Bio-Rad :
8101 - 8150 of 9625 citations for Recombinant Human EGFR protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Proteins were prepared for SDS-PAGE separation by denaturation with 2x Laemmli sample buffer (Bio-Rad Laboratories; 161-0737) with 355mM β-mercaptoethanol for 5min at 95°C.
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bioRxiv - Molecular Biology 2024Quote: ... under reducing conditions and proteins transferred to nitrocellulose membranes using a semidry Trans-Blot Turbo Transfer System (Bio-Rad). Antibody reactions were carried out in 1% skimmed milk in PBS with 0.1% Tween-20 and washed in PBS with 0.1% Tween-20 ...
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bioRxiv - Microbiology 2023Quote: Proteins from LEVs and SEVs were separated on a precast 4-15 % SDS-PAGE (Bio-Rad, Hercules, CA USA) and stained with Coomassie G-250 ...
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bioRxiv - Cell Biology 2024Quote: ... The pelleted protein G Sepharose beads were then resuspended in 50 µl of 2x Laemmli sample buffer (Bio-Rad) and proceeded to SDS-PAGE and immunoblotting analysis.
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bioRxiv - Immunology 2024Quote: ... Total protein concentrations were measured using Pierce BCA Protein Assay Kit and equal amount of protein (10-20μg) mixed with Laemmli sample buffer were loaded in 4-20% TGX precast gels (Biorad) for gel electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amount of protein samples was separated on SDS-PAGE gel (12%) electrophoresis and transferred to nitrocellulose membrane (BioRad). Blots were blocked with 5% skim milk for 1 hour at room temperature and probed with antibodies at 4℃ overnight against the desired proteins ...
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bioRxiv - Cell Biology 2024Quote: Anti-Flag antibody precipitated proteins in the concentrated eluate were resolved in a 4-15% Criterion TGX gel (BioRad) and stained by Colloidal Blue dye (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were transferred onto membranes using Trans-Blot® TurboTM RTA Midi 0.2 µm Nitrocellulose Transfer Kit (Biorad, #1704271). The membranes were subsequently blocked in TBST solution (200 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins in cell lysates were heated at 95[ for 5 minutes in Laemmli samples buffer (Bio-Rad, Hercules, CA), resolved by 10% SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were separated on Mini-PROTEAN® TGX™ Precast Gels (4–20% gradient, BioRad Cat. No. 4561091) in Tris-SDS-glycine buffer ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were transferred to nitrocellulose membranes (GE Amersham) by semi-dry transfer for 10 min at 15V (Bio-Rad), air dried ...
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bioRxiv - Immunology 2024Quote: ... and an equal amount of protein was resolved in 4-12% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) (Bio-Rad). Proteins were transferred to polyvinylidene difluoride membranes (Millipore ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins (20 μg per sample) were separated on Mini-PROTEAN TGX 4–15% precast SDS-PAGE gels (Bio-Rad) for 1–3 hours at 100 V and then transferred to PVDF membrane (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... Elution fractions containing pure protein were determined via SDS-PAGE analysis on a 4-20% gradient gel (Bio-Rad), pooled ...
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bioRxiv - Biochemistry 2024Quote: ... The resolved proteins were transferred onto nitrocellulose membranes (Amersham) through a Trans-Blot semi-dry transfer cell (Bio-Rad). Membranes were subsequently blocked in Tris-buffered saline plus 0.1% Tween (TBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... Protein band intensities were quantified using ChemiDoc MP Imaging System and Image Lab software (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Cell Biology 2024Quote: ... The gel in Figure 7B was run on a 12% Mini-Protean TGX precast protein gel (Bio-Rad, #4561034), whereas the one in Figure 7C was run using hand-cast gels ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then analyzed by 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad™) alongside size standards (Precision Protein Plus ...
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bioRxiv - Neuroscience 2023Quote: ... Separated proteins were blotted on a polyvinylidine fluoride (PVDF) membrane with a Trans-Blot Turbo Transfer System (Bio-Rad). The protein coated membrane was blocked with 5% normal serum (Sigma ...
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bioRxiv - Genetics 2023Quote: ... ∼25 μL sample was run out on a 7.5% SDS-PA at 150V for ∼1 h alongside a protein standard (BioRad Precision Plus Protein Dual Color Standard) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Soluble proteins contained in the supernatant were separated and mixed with 4x Laemmli sample buffer (Bio-Rad, 161-0747) before being loaded on a SDS-PAGE gel (4–20% Criterion™ TGX Stain-Free™ Protein Gel ...
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bioRxiv - Physiology 2023Quote: ... stain-free gels were imaged following UV exposure to visualise total protein content (ChemiDoc MP Imaging System, Bio-Rad). Proteins were transferred from the gel to an ethanol-immersed PVDF membrane in transfer buffer (30 min ...
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bioRxiv - Microbiology 2023Quote: ... The protein samples (10 μL each) were run on 4-20 % Mini-PROTEAN TGX Stain-FreeTM precast Gels (BioRad) in 1× TGX buffer and then transferred to nitrocellulose membrane using a Trans-Blot® Turbo™ Transfer System (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... 25 µl cell lysate and 50 µg protein for tissue lysates was loaded on 4-15% (Bio-Rad, 5678084) and 10% TGX Stain-Free Protein Gels (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to nitrocellulose membranes via semi-dry transfer (Bio-Rad Trans Blot Turbo; 25 V, 30 min) for western immunoblot analysis ...
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bioRxiv - Neuroscience 2023Quote: ... stain-free gels were imaged following UV exposure to visualise total protein content (ChemiDoc MP Imaging System, Bio-Rad). Alternatively ...
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bioRxiv - Immunology 2023Quote: ... and Tris-Glycine SDS buffer (Geneflow B9-0032) or 4–12% Criterion™ XT Bis-Tris Protein Gels (BioRad) and XT MES running buffer (BioRad 1610789) ...
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The effects of caloric restriction on adipose tissue and metabolic health are sex- and age-dependentbioRxiv - Physiology 2023Quote: ... and equal amounts of protein (13 µg per lane) separated on gradient polyacrylamide gels (Bio-Rad, Hercules, CA, USA). Samples were then transferred to Immobilon-FL transfer membrane (Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... The samples (22.5 µg – 85 µg) were loaded on 4-20% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad), ran for the first 30 minutes at 70 V and the next 4 hours at 100 V in Tris-Glycine SDS buffer ...
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bioRxiv - Microbiology 2023Quote: ... Lysed cells were resolved on a 4% to 20% Mini-Protean TGX precast protein SDS-PAGE gel (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: Denatured parasite proteins were resolved on 10% SDS-polyacrylamide gel and transferred to 0.2 μm nitrocellulose membranes (Bio-Rad) using wet-transfer in Towbin buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: Samples were prepared mixing 20μg of protein from each sample with 6.25μL of Laemmli Buffer 4X (Biorad, Hercules, USA), 1.25μL β-mercaptoethanol (BME ...
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bioRxiv - Microbiology 2023Quote: ... The sample was pre-cleared via incu-bation with 30 µl of SureBeads Protein A magnetic agarose beads (BioRad) for 30 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated on the 10% SDS-PAGE gel (Hangzhou Fudebio) and transferred onto the PVDF membranes (Bio-rad). Membranes were blocked in the blocking buffer (Beyotime Biotechnology) ...
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bioRxiv - Molecular Biology 2023Quote: ... the concentration was calculated by the Bradford assay using a Quick Start Bradford Protein Assay Kit 2 (Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: Fractionation of nuclear and cytoplasmic fractions from Drosophila lysates was conducted with the ReadyPrep Protein Extraction Kit (Bio-Rad). Seven whole flies per group were homogenized in cytoplasmic extraction buffer (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 μg of protein was loaded on a Mini-PROTEAN TGX gel 4-20% gradient pre-cast gels (BioRad). Protein was transferred to 0.45 μm nitrocellulose membrane (Biorad ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated using SDS-PAGE and transferred to nitrocellulose membrane using Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked in 5% skimmed milk diluted in Tris-buffered saline with 0.1% Tween-20 (TBS-T ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were resolved by SDS-polyacrylamide gel electrophoresis using 4-15% Mini-PROTEAN® TGX™ gels (BioRad; #4561086) and transferred to nitrocellulose membranes (BioRad ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant containing the protein was filtered (0.45 µm) and applied on an IMAC Ni2+-NTA column (Bio-Rad) for FPLC purification ...
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bioRxiv - Biophysics 2023Quote: ... the Ni-NTA purified protein was buffer exchanged into the storage buffer over a 10-DG column (Bio-Rad) and was stored at 4 °C ...
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bioRxiv - Genetics 2023Quote: Protein extractions were freshly prepared under denaturing conditions by first diluting samples in 4x Laemmli Sample Buffer (Biorad #1610747) containing 50mM β-mercaptoethanol (BME ...
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bioRxiv - Microbiology 2023Quote: ... 25 µl of non-boiled protein sample were loaded on 12% Mini-Protean TGX pre-cast gels (Bio-Rad) and the proteins separated by SDS-PAGE ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were separated by gel electrophoresis in 12% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, 4561043) and transferred to membranes ...
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bioRxiv - Neuroscience 2023Quote: ... 15µg of protein lysate was loaded into a 4-20% polyacrylamide Criterion TGX stain-free gel (Bio-Rad, #5678095). Electrophoresis was performed using 60V for one hour followed by 125V until finished ...
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Functional characterization of ATP13A2 variants associated with distinct neurodegenerative disordersbioRxiv - Molecular Biology 2023Quote: ... 10 μg of protein was loaded on a NuPage 4-12% Bis-Tris gel (Thermo Fisher Scientific, Bio-Rad) and separated during an electrophoresis run at 150 V in MES running buffer (Thermo Fisher Scientific) ...
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: Protein extracts were run on sodium dodecyl sulphate (SDS)-polyacrylamide gels and transferred to nitrocellulose membranes (Turbo blot, Biorad). Membranes were blocked for 2 hours in 5% non-fat milk (BioRad ...
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bioRxiv - Immunology 2023Quote: ... 0.5ug for the IgA blot and 5ug for the SC blot of LCTG-002 was loaded on a 7.5% pre-casted Gel Mini Protein gel TGX (BioRad) in native gel buffer ...
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bioRxiv - Immunology 2023Quote: ... Proteins were then electro-transferred onto PVDF (polyvinylidene fluoride) membranes with Trans-Blot Turbo transfer system (Bio-Rad Laboratories). Antibodies used for the detection are listed in Table S1 ...
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bioRxiv - Plant Biology 2023Quote: ... Leaf lysates were cleared by centrifugation at 10,000×g for 5 min and total protein normalized via Bradford Assay (Biorad). Extracts were prepared for SDS-PAGE with a 5x loading buffer containing 10% (w/v ...