Labshake search
Citations for Bio-Rad :
8051 - 8100 of 9407 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription (RT) was performed using 250 ng of DNase-treated RNA using the iScript cDNA synthesis kit (Bio-Rad Cat#1708890) and the resulting RT reactions were diluted 1:5 with nuclease-free water ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed and homogenized as described elsewhere.30 Protein concentration was determined using a DC protein assay kit (Bio-Rad, 5000116). 10-15 μg of protein was boiled at 95°C for 5 min in Laemmli buffer (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... Cell culture supernatants were screened for the presence of 27 human cytokines and chemokines using the Bio-Plex Pro Human Cytokine Standard 27-Plex kit (Bio-Rad Laboratory) on a FLEXMAP 3D® analyzer (Luminex ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified and normalized PCR products were further pooled into a single library and quantified using qPCR (KAPA Library Quant Kit qPCR Mix, Bio-Rad iCycler). The final library was diluted to 4 nM and sequenced in an Illumina HiSeq 2500 Platform using a 2×250bp RapidRun kit for an average coverage of 160,000 paired reads per PCR product.
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bioRxiv - Immunology 2020Quote: ... Immunoblots were conducted using Mini-Protean TGX stain-free gels and Transfer-Blot Turbo RTA Transfer Kit reagents(Bio-Rad, Mississauga, Ontario). ABCF1 protein was probed with primary anti-ABCF1 antibody (HPA017578 ...
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bioRxiv - Microbiology 2022Quote: ... The amplification kinetics of each product were checked at the end of each cycle by measuring the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad, France). The results were analyzed using Bio-Rad CFX Maestro software ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the cDNA was with Taq Pro Universal SYBR qPCR Master Mix kit (Q712, Vazyme, Nanjing, China) by the CFX96TM Real-Time System (BIO-RAD, USA). The thermal cycles were 95 ℃ for 5 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The fixed and stained gene expression slide was treated with a permeabilization enzyme specific for mouse tongue determined by the Spatial tissue optimization slide kit (10x Genomics, #1000191) for 24 minutes on the thermal cycler (Bio-Rad, 1851197). Synthesis of cDNA and second strand were performed using reverse transcriptase and second strand reaction (primer and enzyme) ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was extracted from cardiac cultures after 4 days on either soft or stiff PDMS (n=4) using the AurumTM Total RNA Mini Kit (Bio-Rad Laboratories). Libraries were constructed by the Purdue Genomics Core Facilities according to standard protocols using TruSeq Stranded mRNA Library Prep Kit (Illumina) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... DNase treatment was performed prior to cDNA synthesis with the iScript™ gDNA Clear cDNA Synthesis Kit (#1725035, Biorad, Mississauga, ON Canada). Quantitative real-time PCR (qPCR ...
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bioRxiv - Genomics 2019Quote: ... Protein concentration was measured using the RC DC (reducing agent and detergent compatible) Protein Assay Kit II (5000122, Bio-Rad; Hercules, CA). For qPCR experiments ...
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Liver X Receptor activation regulates genes involved in lipid homeostasis in developing chondrocytesbioRxiv - Physiology 2019Quote: ... Complimentary DNA (cDNA) synthesis was conducted using the iScript™ cDNA Synthesis Kit with 500 ng of starting RNA (Bio-Rad Laboratories), and combined with 50 μM of forward and reverse primers (for primer sequences ...
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bioRxiv - Cancer Biology 2019Quote: ... Isolated RNA (100 ng) was subjected to qRT-PCR analysis using iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad) performed on the 7500 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Bioengineering 2019Quote: ... For equal loading the protein concentration of the supernatant was determined with BioRad DC protein assay kit II (BioRad#5000116, Cressier, Switzerland) and standard curve with Albumin (ThermoFisher #23210 ...
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bioRxiv - Microbiology 2019Quote: ... and ampR was measured by quantitative Reverse Transcriptase PCR (qRT-PCR) using the iTaq Universal SYBR Green One-Step kit (Biorad, Hercules, CA) and a CFX96 Touch Real-Time PCR detection system (Bio- Rad ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the membrane was washed three times with TBST and detection was performed with the Clarity™ Max ECL kit for pulldown experiments (Bio-Rad) and Clarity™ ECL kit for all other experiments (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... This step was repeated two times and the protein concentration was determined by the DC Protein Assay Kit (Bio-Rad, Munich, Germany) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... and cDNA was synthesized from 500 ng of RNA using the iScript kit according to the manufacturer’s protocol (Bio-Rad, 170-8891).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... DNase treatment was performed prior to cDNA synthesis with the iScript™ gDNA Clear cDNA Synthesis Kit (#1725035, Biorad, Mississauga, ON, Canada). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Biorad ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was then generated using the Superscript™ III Reverse Transcriptase kit and pre-amplified with a mixture of the tested TaqMan® probes (Table S1) using the SsoAdvanced™ PreAmp Supermix (Biorad). Differential levels of cDNA expression were measured using the droplet digital PCR (ddPCR ...
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bioRxiv - Microbiology 2019Quote: ... and FLAG-cMCF-HA recovered as described above were prepped using the Bio-Rad ReadyPrep 2-D Cleanup Kit (Bio-Rad 1632130). The sample was then loaded onto 11 cm pH 3-10 immobilized pH gradient (IPG ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative RT-PCR was performed by using a Universal SYBR® Green kit and the C1000 Touch Thermal Cycler real-time PCR detection system (Bio-Rad). The EF1α (AT5G60390 ...
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bioRxiv - Physiology 2019Quote: ... Protein concentration was determined in triplicate using a commercial colorimetric assay (Bio-Rad Protein Assay kit-II; Bio-Rad, Gladesville, NSW, Australia). Nuclear and cytosolic fraction enrichment was confirmed by blotting the separated fractions against a nuclear (histone H3 ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was prepared from RNA isolated from the cell lysates using a iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA), and qRT-PCR was conducted as described previously (21 ...
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bioRxiv - Cell Biology 2019Quote: ... A total of 100 ng RNA per sample was subjected to Reverse Transcriptase (RT) by using iScriptTM cDNA Synthesis kit (1708890, BioRad, Hercules, CA). For whole liver ...
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bioRxiv - Physiology 2020Quote: ... The total RNA was purified from genomic DNA contamination then converted to cDNA using iScriptTM gDNA Clear cDNA synthesis kit according to manufacturer’s instructions (Bio-Rad, CA, USA). Commercially available SYBR® Green Glucose Metabolism Array consisting of 84 genes were used (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... Densitometry of the blots was detected using the Enhanced Chemiluminescence Kit HRP (SQ101, EpiZyme, China) in the ChemiDocTM MP Imaging System (Bio-Rad, USA). Information on the primary antibodies used has been listed and provided (Table EV4).
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was exposed using the Enhanced Chemiluminescence Kit HRP (SQ101, EpiZyme, China) in the ChemiDocTM MP Imaging System (Bio-Rad, USA).
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bioRxiv - Molecular Biology 2020Quote: ... The product from the final elution and whole cell lysate supernatant were subjected to silver staining using Silver Stain Plus Kit (BIO-RAD, # 1610449) to check pull down efficiency and purity ...
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bioRxiv - Molecular Biology 2021Quote: ... and quantitative PCR (qPCR) was performed to quantify mRNA abundance using a SsoFast™ EvaGreen® Supermix kit (Bio-Rad, 172-5204,) on a Bio-Rad CFX96 cycler ...
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bioRxiv - Immunology 2020Quote: ... Multiplexed cytokine measurements on macrophage cell culture supernatants were performed using a custom Bio-Plex Express Human Cytokine kit (Bio-Rad 17004073) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Cytokine secretion assay was performed using ThermoFisher ProcartaPlex Multiplex Custom Made mouse cytokine 5-plex assay kit following manufacturer’s instructions and analyzed with the Bio- Plex 200 Luminex-based multiplex analysis system (Bio-Rad, Hercules, CA). All samples were analyzed using technical duplicates ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Reverse transcription reactions were performed to synthesize cDNA from 200 ng of total RNA using the iScript™ Select cDNA Synthesis Kit (Biorad, CA). Real-time quantitative PCR (qRT-PCR ...
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bioRxiv - Neuroscience 2021Quote: ... An aliquot was taken to measure radioactivity and protein content using a liquid scintillation counter and a DC protein assay kit (Bio-rad, USA), respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... Gels were stained using the Silver Staining Plus Kit and documented on the ChemiDoc XRS+ system (Bio-Rad Laboratories Inc., Hercules, USA) according to the manufacturers protocol.
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bioRxiv - Genomics 2020Quote: ... the membrane was incubated with peroxidase-conjugated goat anti-mouse IgG and proteins were detected using a chemiluminescence detection kit (Super-Signal West Pico, Pierce) and a ChemiDoc™ MP system (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... and transferred to a 0.20μm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad, Marnes-la-Coquette, France). The membranes were blocked for 30□min in 5% low-fat milk in TBS-0.1% Tween 20 at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The immunoblotting signals were detected using a CCD camera (Intas Biopharmaceuticals, Ahmedabad, India) after application of enhanced chemiluminescence detection kit (Clarity™ Western ECL Substrate; Bio-Rad, Hercules ...
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bioRxiv - Genetics 2022Quote: ... qRT-PCR analysis were performed with iQ-SYBR Green Supermix kit in an iCycler iQ5 system following the instructions of the manufacturer (Bio-Rad, USA). The relative quantifications of mRNA levels were performed using the cycle threshold (Ct ...
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bioRxiv - Immunology 2022Quote: ... Oligo-dT first strand cDNA synthesis was performed from 5 μg total RNA with iScript Advance cDNA Synthesis Kit for RT-qPCR (Bio-Rad; #1725038) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... The blot was then washed in 6x 5 minutes of TBST prior to being developed with Clarity™ Western ECL Substrate kit (Bio-Rad). To examine actin levels as a loading control blot was stripped using Restore™ WesternBlot Stripping Buffer (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... 500 ng of total RNA was used in a 20 µL reaction volume with the Bio-Rad iScript cDNA synthesis kit (Bio-Rad, #1708890) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... the fluorescence of the EvaGreen dye incorporated into the PCR product was measured at the end of each cycle using SoFast EvaGreen Supermix 2× kit (Bio-Rad, France). A standard curve using gDNA of purified viruses was performed in parallel of each experiment ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription of the RNA was performed to synthesize cDNA following the standard manufacturer’s instructions of the iScript Reverse Transcription Supermix for RT-qPCR kit (Bio-Rad PN 1708841). cDNA was submitted to the University of North Carolina Chapel Hill Advanced Analytics Core (UNCAAC ...
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bioRxiv - Neuroscience 2024Quote: ... The signals were detected using an enhanced chemiluminescent (ECL) plus reagent kit (Share-Bio, Cat. SB-WB012L, China) and viewed with a ChemiDoc MP Imaging System (Bio-Rad Laboratories).
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bioRxiv - Zoology 2024Quote: ... and 100 ng of RNA were used for cDNA synthesis with the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad Laboratories, USA), resulting in a final concentration of 5 ng/μl for each sample.
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bioRxiv - Microbiology 2023Quote: ... the fluorescence derived from the incorporation of SybrGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoAdvanced Universal SYBR Green Supermix 2X Kit (Bio-Rad, France). The results were analyzed using Bio-Rad CFX Maestro software ...
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bioRxiv - Bioengineering 2024Quote: Four samples of total RNA were collected for each treatment condition using the Aurum Total RNA Mini Kit (Bio-Rad, Hercules, CA). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein pellet was resuspended in UTC buffer (8 M urea, 2 M thiourea, 4% CHAPS) and quantified using RC DC™ Protein Assay Kit (Bio-Rad). Sample with 80 µg proteins resuspended in rehydration buffer (8 M urea ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of RNA was taken to synthesize complementary DNA (cDNA) conversion reactions were done using an iScript™ Advanced cDNA Synthesis Kit (catalog no. 18080085, Biorad, USA) following the manufacturer’s protocol ...