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Citations for Bio-Rad :
8051 - 8100 of 9210 citations for Borrelia burgdorferi sensu stricto B31 ErpN OspE Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Samples were subsequently separated by electrophoresis on precast gels (4-20% mini protein TGX gels, Bio-Rad catalog #456-1094) and transferred to nitrocellulose membranes (Trans-Blot Turbo Transfer ...
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bioRxiv - Plant Biology 2022Quote: ... The eluted protein concentration was estimated using a Bradford assay (Quick Start™ Bradford 1x Dye Reagent #5000205; Bio-Rad).
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bioRxiv - Plant Biology 2022Quote: ... approximately 60-70 μg of protein was loaded onto 7 cm pH 3-10 NL and pH 4-7 IPG strips (BioRad) and subjected to first-dimensional electrophoresis with increasing voltages from 50V to 2000V ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... detergents and undigested protein were removed using a Bio-Scale Mini Macro-Prep High S 1 mL cartridge (Bio-Rad) on an Agilent 1100 HPLC system at 1 mL/min ...
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bioRxiv - Plant Biology 2023Quote: ... An equal amount of protein bands were manually appointed per lane and adjusted in the Lane profile menu of the Image Lab software (BioRad). Band intensity was quantified relative to the unreacted control sample.
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bioRxiv - Neuroscience 2022Quote: ... Absorbance at 450 nm was measured in a plate reader (Thermo) and the total protein content of the samples was obtained by the Bradford assay (Biorad). As GDNF is not expressed in the mouse cortex ...
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bioRxiv - Microbiology 2023Quote: ... the Stain-Free visualization was enabled by activation of the gels for 45 seconds with UV-light before the proteins were transferred by Trans-Blot Turbo Transfer (Biorad) onto a nitrocellulose membrane (Trans-Blot Turbo RTA ...
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bioRxiv - Plant Biology 2023Quote: Protein concentration was measured via Bradford assay using a Quick Start™ Bradford 1x Dye Reagent (Biorad, Cat. No. 5000205) prior to protein content normalization ...
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bioRxiv - Plant Biology 2022Quote: ... Eluted protein samples were checked by SDS-PAGE and Coomassie brilliant blue staining (Bio-Safe™ Coomassie Stain, Bio-Rad). The protein concentration was determined with the Bradford method (Quick Start™ Bradford Reagent ...
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bioRxiv - Neuroscience 2022Quote: ... were resolved by SDS-PAGE using stain-free precast gels (4-15% Mini-Protean Tgx Stain-Free Protein Gels, Biorad). Gels were then activated by Ultraviolet light with the ChemiDocTM Touch Imaging System (Biorad ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein were resolved in SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA) at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... and equal amounts of proteins were subjected to SDS-PAGE (TGX Stin Free FastCast Acrylamid, 12%, Bio-Rad, 161-0185) and transferred onto nitrocellulose membrane (Transblot Turbo ...
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bioRxiv - Cell Biology 2022Quote: ... Total cell lysates were prepared with icecold lysis buffer and the protein concentration was measured by Coomassie binding (Bio-Rad). Equal amounts of protein were added to each well of a G-LISA plate and incubated for 30 min at 4°C ...
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bioRxiv - Physiology 2022Quote: Equal amounts of protein extracts were resolved by SDS–PAGE using 4-15% Mini-PROTEAN TGX precast gel (Bio-Rad) and transferred to PVDF membrane ...
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bioRxiv - Molecular Biology 2022Quote: ... The luminescence was calculated as an average of three technical replicates and normalized to the total protein levels quantified using the Bradford’s method (Bio-Rad). Results are from the two or three biological replicas and are expressed as a percentage of the normalized luminescence of the control.
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bioRxiv - Cancer Biology 2022Quote: ... Eluted proteins separated in 4-20% polyacrylamide gels in denatured conditions were visualized for purity after Coomassie blue (Bio-Rad) staining.
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bioRxiv - Immunology 2022Quote: ... The protein extracts were subjected to appropriate concentrations of SDS–PAGE for electrophoresis and transferred to PVDF membranes (Bio-Rad). Membranes were blocked with 5% bovine serum albumin (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... A representative size-exclusion chromatography chromatogram is shown in Figure S4 with a gel filtration protein standard (Bio-Rad Laboratories). The fractions from the single monomer peak were combined and diluted ten-fold and buffer exchanged three times into 10 mM sodium phosphate buffer at pH 6.5 with an Amicon Ultra-15 Centrifugal Filter Unit Ultracel-10 (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... Cell equivalents of ~1.5×106 cells per lane were separated by 10% SDS-PAGE along with the Precision Plus Protein Kaleidoscope standard (BioRad). The fractionated proteins were transferred to a PVDF membrane ...
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bioRxiv - Plant Biology 2024Quote: ... proteins separated in SDS-PAGE gels were transferred onto a PVDF membrane using Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked in 5% (w/v ...
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bioRxiv - Plant Biology 2024Quote: ... in 0.5 % milk in TBS-Tween 0.1 % to reveal HA-tagged NIN proteins following chemiluminescence revelation using the Clarity Western ECL Substrate (Bio-Rad) and the ChemiDoc Touch imaging system (Bio-Rad).
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured and proteins were separated by SDS-PAGE in Criterion™ TGX Stain-Free™ Gels (Bio-Rad), and transferred onto nitrocellulose membranes with the Trans-Blot® Turbo™ Blotting System (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were resolved by SDS/PAGE and transferred to nitrocellulose membranes using the Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked with 5% BSA in Tris-buffered saline containing Tween 20 (TBS-T ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The protein concentrations in each cell extract were measured with the Bradford assay (SmartSpec Plus spectrophotometer, Bio-Rad, Hercules, CA) using the Bradford dye reagent and bovine standards (BioRad ...
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bioRxiv - Plant Biology 2024Quote: ... Ten microliters of isolated total protein were separated on a 4-20% Tris-glycine stain free polyacrylamide gel (Bio-Rad) at 180 V for one hour in 1X Tris/glycine/SDS running buffer ...
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bioRxiv - Biochemistry 2024Quote: ... the absorbance of standards and protein samples was measured at 490 nm using a microplate absorbance reader (Bio-Rad iMarkTM). See Fig ...
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bioRxiv - Physiology 2024Quote: ... Equivalent amounts of total protein were separated by SDS-PAGE on 4% to 15% Criterion TGX gels (Bio-Rad; 5671083). Megalin and cubilin were detected with anti-megalin antibody generously provided by Dr ...
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bioRxiv - Physiology 2024Quote: ... Protein extracts were denatured at 95 °C for 10 min and separated in 10% SDS-PAGE gels (1610156, Bio-Rad) at 80 V for ∼45 min followed by 110 V for ∼65 min ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were transferred to a hybond ECL nitrocellulose membrane using a wet transfer system (Bio-Rad Laboratories, Hercules, CA, USA). The membrane was incubated in α-Ty antibody (1:10 ...
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bioRxiv - Cancer Biology 2023Quote: All binding assays were performed on a ProteOn XPR36 SPR Protein Interaction Array System (Bio-Rad Laboratories, Hercules, CA, USA). The instrument temperature was set at 25 °C for all kinetic analyses ...
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bioRxiv - Molecular Biology 2024Quote: ... and purity was determined using SDS-PAGE on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) stained with Biosafe Coomassie (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.01% bromophenol blue) and denaturated 5 min at 95°C before protein separation by SDS–PAGE on 4-15% precast gels (Biorad).
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bioRxiv - Microbiology 2024Quote: Equal amounts of Total Protein extracts were migrated on a 12.5% SDS-PAGE gel and transferred onto Immun-Blot PVDF blotting membrane (Bio-Rad). Transfer was performed with the Bio-Rad Transfer System (30 min ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured for 10 minutes at 70°C and proteins were separated (55min, 200V) on a Midi 4-12% Bis-Tris gel (BioRad) in 1x MOPS buffer (BioRad ...
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bioRxiv - Neuroscience 2024Quote: ... The adapted protein quantity per lane was loaded into 4-20% 1.5 mm Tris-glycine 15 well mini gels (Bio-Rad) with 1X Laemmli sample buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of protein were resolved in SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA) at 4°C ...
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bioRxiv - Neuroscience 2024Quote: Samples (8–25 μg protein extract) were run on 8–10% acrylamide gel at 100 Volt in Running Buffer (1X Tris–Glycine-SDS, Biorad) together with Precision Plus Protein Dual Color Standards (Biorad) ...
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bioRxiv - Neuroscience 2024Quote: ... 10-20 μg protein per sample were loaded onto SDS-page gels for protein separation and then transferred onto nitrocellulose or methanol-activated PVDF membranes via full-wet transfer assay (BioRad) or semi-wet transfer ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to a PVDF membrane using the Bio-Rad Turbo Transblot System and transfer kit (Bio-Rad Laboratories) and fixed in methanol prior to blocking in 5% non-fat milk in TNT buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: Protein samples were incubated at 95°C for 10 minutes before being resolved on TGX Stain-free gels (Bio-Rad). The proteins were transferred onto 0.2 μm nitrocellulose membranes using Trans-Blot Turbo Transfer (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... Buffer containing eluted protein was filtered through a 0.22-μm spin filter and directly loaded on the Enrich SEC650 column (Bio-Rad) for SEC ...
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bioRxiv - Biophysics 2023Quote: ... proteins were transferred to PVDF membranes using a Trans-Blot Turbo RTA Mini 0.2 μm PVDF Transfer Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Protein separations were transferred onto a nitrocellulose membrane using the Trans-blot Turbo RTA transfer kit (Bio-rad, #170-4270) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... Each column was calibrated using 660 kDa to 1.35 kDa molecular weight protein standards (thyroglobulin, IgG, ovalbumin, myoglobin, and vitamin B12) (Bio-Rad). Following peak analysis by SDS-PAGE ...
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bioRxiv - Neuroscience 2024Quote: ... Stain-free protein gels were activated with UV light for 5 min and then transferred to PVDF membranes (Bio-Rad) using a turbo transfer system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... and then proteins were transferred to a nitrocellulose membrane using a wet Trans-Blot system (Bio-Rad, Hercules, CA, USA). The primary antibodies used were anti-slow MyHC (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... 7.5 mg of proteins was diluted in ChIP buffer supplemented with 0.01% SDS and precleared for 1 hr at 4°C with 50 μl of SureBeads Protein A Magnetic Beads (BioRad) and 100 μg bovine serum albumin (BSA) ...
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bioRxiv - Physiology 2023Quote: ... Protein extracts were denatured at 95 °C for 10 min and separated on 10% SDS-PAGE gels (1610156, Bio-Rad) at 80 V for ∼40 min followed by 110 V for ∼70 min ...
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bioRxiv - Cell Biology 2024Quote: ... 30µg of total proteins were loaded onto each well of Mini-PROTEAN TGX Stain-Free Precast Gels (10%, Biorad, # 4568031), and migrated for 45 min at 120 Volts ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were resolved by electrophoresis through 12% agarose polyacrylamide gels and then transferred to PVDF membranes (Bio-Rad Cat #1620175) by electroblotting ...