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Citations for Bio-Rad :
8001 - 8050 of 9210 citations for Borrelia burgdorferi sensu stricto B31 ErpN OspE Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... and equal volume and quantity of protein samples were made by addition of 4x Laemmli Sample Buffer (#1610747, Bio-Rad) containing 2-merchatoethanol (#1610710 ...
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bioRxiv - Cancer Biology 2020Quote: ... Approximately 20 mg of total protein were loaded for electrophoresis into 4–20% sodium dodecyl sulfate polyacrylamide gels (Bio-Rad). Separated proteins were transferred to a 0.45 μm nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Proteins were separated by 4-15% SDS-PAGE gel (Bis-Tris Criterion™ TGX™ pre-cast gels, Bio-Rad) and transferred to PVDF membranes (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... Supersignal West Femto (Pierce) incubation was used to visualize the protein bands of interest on the ChemiDoc XRS+ Imaging system (Biorad). Images acquisition and densitometry analysis was performed using the Image Lab software (Biorad).
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bioRxiv - Cancer Biology 2020Quote: ... Around 40 μg of whole-cell lysate was resolved in 10% SDS-PAGE gel (Mini protein Tetra cell Bio-Rad) and blotted to nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... and the separated proteins were then transferred to a polyvinylidene difluoride (PVDF) membrane using a wet transfer cell (Bio-Rad) at 100 mAmps at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... The immunodetected protein bands were then analyzed using ChemiDoc XRS system with Quantity One software (Bio-Rad, Richmond, CA, USA).
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bioRxiv - Cancer Biology 2021Quote: ... thawed and centrifuged at full speed in an Eppendorf microcentrifuge for 15 minutes and protein content was assessed by Bradford assay (Biorad). Cell lysate equivalent to 1 mg protein collected 36 hours post-transfection was diluted to 500 μl in TGH buffer for each immunoprecipitation reaction and precleared with 10 μl sepharose-protein G beads (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... and 30 μg of total protein were separated by 10% SDS-PAGE and transferred to a nitrocellulose membrane (Bio-Rad). Correct protein loading was confirmed by Ponceau staining ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples from total or immunopurified ribosomes or aliquots from TCA precipitated sucrose gradient fractions were run in 1X Laemmli buffer on 4-20% Mini-PROTEAN TGX Precast Protein Gels (BioRad) in 1X Tris/Glycine/SDS buffer (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... Samples were boiled with SDS sample buffer and resolved on a 10% Mini-PROTEAN TGX Precast Protein Gel (BIO-RAD). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Neuroscience 2021Quote: Soluble protein cellular lysates (20μg) were analysed by 4-15% gradient SDS-PAGE and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked with 5% skim milk in 1 x PBS (Invitrogen ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: For blotting the SDS/PAGE-separated proteins were transferred to a PVDF membrane (Amersham Hybond P0.2 PVDF, 55 GE) by semiwet transfer (Bio-Rad) and blocked for 1 h with PBS/2% milk.
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bioRxiv - Immunology 2021Quote: ... Luminex beads were coupled to the Spike protein as previously described (63) and added to a Bio-Plex plate (BioRad). Beads were washed with PBS 0.05% tween using a magnetic plate washer (MAG2x program ...
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bioRxiv - Immunology 2020Quote: ... first Protein A/G agarose was loaded on Micro Bio-Spin™ Chromatography Columns (Bio-Rad Laboratories, Hercules, CA, USA). After gravity flow ...
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bioRxiv - Microbiology 2021Quote: hCMP-mRBD protein kinetic binding studies to ACE2-hFc and CR3022 antibody were performed on a ProteOn XPR36 Protein Interaction Array V.3.1 (Bio-Rad). The GLM sensor chip was activated with sulfo-NHS and EDC (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... coated with PBS alone or with 2 µg/ml of SARS-CoV-2 S protein in PBS overnight at 4°C were blocked for 1 hour with 3% nonfat dry milk (Biorad) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... and vasopressin) peptides were spotted onto a 0.2-μm polyvinylidene fluoride membrane (Immun-Blot PVDF Membrane for Protein Blotting, BIO-RAD). The membrane was air dried at room temperature and was washed for 10 min in 0.05 M Tris buffer (pH 7.6 ...
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bioRxiv - Cell Biology 2021Quote: ... gels were subjected to a western blot in which protein was transferred onto a 0.45 μm nitrocellulose membrane using a Mini Trans-Blot Cell (Bio-Rad), in transfer buffer in the presence of an ice block for 1 hour at a constant current of 255 mA ...
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bioRxiv - Genomics 2021Quote: ... centrifuged at 14,000 rcf for 20 min at 4°C and protein amounts in the supernatant determined using the Bradford assay (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Antibodies were affinity purified using either the peptides linked to SulfoLink beads (Pierce) or the Ascaris proteins (without GST) linked to a mix of Affigel-10/15 (BioRad).
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bioRxiv - Cell Biology 2021Quote: ... Samples were prepared in Licor protein sample loading buffer and run on TGX 7.5% and 4-20% Tris-glycine gels (Bio-Rad) and blotted onto a nitrocellulose membrane ...
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bioRxiv - Genetics 2020Quote: ... 10 µg of protein were loaded in each well of a gel (Bio-Rad # 567-1094, 567-1095, 456-1036). Blots were blocked in 5% milk/TBST for two hours at room temperature (RT) ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed in RIPA buffer and protein extracts from equal numbers of cells were resolved on a 12% SDS-PAGE gel (Biorad), followed by transfer onto Immun-Blot PVDF membranes (Biorad) ...
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bioRxiv - Bioengineering 2020Quote: ... after which proteins were transferred to polyvinylidene fluoride (PVDF) membranes (catalog no. 162-0177; Bio-Rad Laboratories; Hercules, CA, USA). Membranes were blocked with 5% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... and equal protein amounts were boiled at 95°C for 10 minutes prior to being run on a gel (BioRad) at 120V ...
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bioRxiv - Cell Biology 2022Quote: ... 4-8 µL of samples and 3 µL of Precision Plus Protein Dual Color Standard (cat. no. 1610374, Bio-Rad) were run on 4-12% Bis-Tris Bolt gels (Thermo Fisher ...
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bioRxiv - Biochemistry 2022Quote: ... the amount of GFP-bound protein was assessed by loading final extract in sample buffer (without boiling) on a Mini-PROTEAN TGX Precast Gel (BioRad), visualised with LED488 illumination (G-box ...
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bioRxiv - Cancer Biology 2022Quote: ... 40 μg of total protein was separated on a 4–20% SDS-PAGE gel (mini-PROTEAN TGX gels, Bio-Rad) along with PageRuler Prestained protein ladder (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... Western blots were performed with 4-20% Mini-PROTEAN(R) TGX(tm) Precast Protein Gels (Bio-rad, #4561095 or #4561096) and Trans-Blot ® Turbo™ Mini PVDF Transfer Packs (Bio-rad #1704156) ...
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bioRxiv - Bioengineering 2022Quote: ... and the nanocluster size was derived by eluting 20.0 μL of the sample with PBS buffer at a flow rate of 0.5 mL/min and calibrating the chromatograph with that of the protein standards (Bio-Rad). The hydrodynamic size and zeta potential of supraclusters were determined using a Zetasizer NANO ZS-90 instrument (Malvern) ...
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bioRxiv - Biochemistry 2022Quote: ... proteins in gels were transferred to a PVDF membrane using a Mini Trans-Blot® Electrophoretic Transfer Cell (Bio-Rad) and blocked with 5% milk ...
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bioRxiv - Cell Biology 2022Quote: ... Eluted samples and whole cell lysate controls were loaded and run on a 10% Mini-PROTEAN TGX protein gel (Biorad), visualized with AcquaStain (Bulldog Bio) ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were centrifuged at 14,000g for 15 min at 4°C and supernatant protein concentrations were determined by Bradford assay (Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... proteins were transferred to nitrocellulose membrane from SDS- or native-PAGE gels using the Transblot Turbo semidry transfer apparatus (BioRad) at 25V for 30 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were boiled with SDS-PAGE sample loading buffer (6X SDS) and 30 ug of protein was loaded to each well of a 10% Tris-glycine SDS-PAGE gel (BioRad). Proteins were transferred to a PVDF membrane (Millipore) ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein (15 μg) were loaded on 4-15% 1 D polyacrylamide Mini-PROTEAN TGX Stain-Free gels (BioRad) and run in 1X Tris Glycine SDS buffer (BioRad ...
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bioRxiv - Cancer Biology 2022Quote: Quantification of protein levels from western blot bands was done using the Evolution-Capt Edge software (Vilber) or ImageLab software (Biorad). The data presented are expressed as means ± SD ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... The protein extract was solubilized in 2-D lysis buffer (30 mM Tris-HCl pH 8.8, containing 7 M urea, 2 M thiourea and 4% CHAPS. Protein concentration was measured using Bio-Rad protein assay method ...
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bioRxiv - Cell Biology 2022Quote: ... 30 and 36 hpi respectively on a 4–15% Mini-PROTEAN® TGX™ Precast Protein Gel (4561083, Bio-Rad). Blotting was performed using the Trans-Blot Turbo Mini 0.2 μm Nitrocellulose Transfer Pack (1704158 ...
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bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE fractionated proteins were transferred to a nitrocellulose membrane using a semidry Trans-Blot Turbo Transfer System (Bio-rad). Membranes were blocked in 10% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... Extracted proteins (25-35 μg) were separated by 8-12% SDS-PAGE and transferred onto polyvinylidene difluoride membranes (PVDF, BioRad). Membranes were blocked for ~30 min in PBST (PBS and 0.1% Tween 20 ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The solution was centrifuged 16,000g for 10 minutes at 4°C and the supernatant containing the mitochondrial or cell lysate proteins quantified (Bio-Rad DC Protein Assay ...
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bioRxiv - Genetics 2022Quote: ... the antibodies were prepared by mixing 3 ug of antibody HNRNPK and IgG (as a negative control in a separate tube) with 650 ul of Dilution Buffer and 50 ul of Surebeads Protein G magnetic Beads (BioRad) and left in rotation for 30 minutes at 4 °C ...
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bioRxiv - Synthetic Biology 2022Quote: ... Ribosome fractions were resolved by SDS-polyacrylamide gel electrophoresis on 10% Mini-PROTEAN TGX™ Precast Protein Gels (Bio-Rad). Prior to loading ...
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bioRxiv - Physiology 2022Quote: ... the electrophoresed proteins were transferred from SDS-agarose gel to a nitrocellulose membrane (Bio-Rad Laboratories, Inc., Hercules, CA, USA) with a semi-dry western blot system (Pierce Power Blotter ...
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bioRxiv - Neuroscience 2022Quote: 10 μL of tissue samples (25 μg of protein per well) were placed into each well of the Western blot gel (Biorad Criterion TGX Stain free 4-15% 18 wells ...
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bioRxiv - Microbiology 2022Quote: ... Equal amounts of total protein were loaded on a 4-20% gradient SDS-PAGE gel (Mini Protean TGX, Bio-Rad), then blotted onto nitrocellulose membranes and probed with anti-villin mouse monoclonal antibody (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... His6 tagged proteins were detected with anti-His6 primary antibody (Pierce; 1:6,000) and antimouse secondary antibody (Biorad; 1:10,000). GroEL was used as cytoplasmic control and detected as above ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately 20 μg of total proteins in tissue lysates were separated on electrophoresis using 4 - 12% gradient SDS-polyacrylamide gels (BioRad) and transferred to the PVDF membrane (BioRad) ...