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Citations for Bio-Rad :
7951 - 8000 of 9210 citations for Borrelia burgdorferi sensu stricto B31 ErpN OspE Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Bound proteins were washed 3 times in 500 mM KCl and eluted on Bio-spin disposable chromatography columns (Bio-Rad) with flag peptide as described in (88) ...
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bioRxiv - Physiology 2020Quote: ... 10-25 µg of total protein was loaded in a 15-well pre-casted gradient gel (Bio-rad, 456-8086). After electrophoresis ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were separated by denaturing electrophoresis in 12% SDS-polyacrylamide gels and transferred to a nitrocellulose membrane (Bio-Rad) with a Mini-Protean Tetra Vertical Electrophoresis Cell at 100V for 1 h at 4°C ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... either on 7-12% Tris-glycine gels or gradient NuPAGE 4-12% Bis-Tris Protein Gels (Thermo Fischer) or gradient Criterion TGX gel (4-15%) (BioRad). List of antibodies and their working dilution used in this work are reported in (Table S4) ...
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bioRxiv - Cell Biology 2019Quote: ... SDS dye was added to a 1X concentration and lysates (equal µg of protein) were loaded onto 4-20% Tris-glycine gels (BioRad). Gels were transferred to nitrocellulose ...
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bioRxiv - Synthetic Biology 2020Quote: ... 5 or 10 μL of the supernatant were resolved on an Any kD TGX Stain-Free protein gel (4568126, BioRad) alongside a Chameleon Duo Pre-Stained Protein Ladder (928-60000 ...
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bioRxiv - Molecular Biology 2020Quote: ... was used at a dilution of 1:10000 for overnight at 4°C and probed for protein using ECL chemiluminescence kit (BioRad). HPLC purified protein was taken as control ...
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bioRxiv - Physiology 2020Quote: ... The supernatant was collected following centrifugation at 8,000g for 10 min and protein concentrations were determined in triplicate using the Bradford method (Bio-Rad). Ten micrograms of protein were subjected to SDS-PAGE on 4–20% Criterion TGX Stain-Free Protein Gel (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: Size exclusion chromatography for MEZ was run on a S200 HiLoad 16/600 column at 0.5mL/min with protein standards from Bio-Rad to determine the oligomeric state of MEZ in solution ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 10 min at 21,000 × g at 4°C and Bio-Rad protein assay reagent (Bio-Rad) was used to determine the protein concentrations of lysates ...
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bioRxiv - Developmental Biology 2019Quote: ... NuPage 4-12% Bis-Tris protein gels beofre transferring into PVDF membranes using the Trans-Blot Turbo transfer system (Biorad). Prior to blocking and antibody incubation ...
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bioRxiv - Immunology 2020Quote: ... Equal volumes of 30 μg total protein were denatured by adding Sample Loading Buffers (BIO-RAD Laboratories; Hercules, CA, USA). Denatured protein samples were loaded onto 4-20% Tris-Glycine Gel Novex WedgeWell (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... 12.5-50 μg of protein was mixed with one third volume of 4X Laemelli Sample Buffer (Bio-Rad Laboratories 1610747) and boiled at 100 °C before loading into a polyacrylamide gel alongside either the PageRuler™ or PageRuler™ Plus Prestained Protein Ladder ...
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bioRxiv - Microbiology 2019Quote: ... Beads were boiled in 1X Laemmli Sample Buffer and proteins were analyzed by SDS-PAGE and Bio-Safe Coomassie staining (Biorad).
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bioRxiv - Microbiology 2020Quote: Proteins were separated by SDS-PAGE and then transferred on PVDF membranes using the Trans-Blot Turbo blotting system (Biorad). Pyocins S5 and AP41 were detected using rabbit specific polyclonal affinity-purified ɑ-S5 and ɑ-AP41 antibodies ...
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bioRxiv - Microbiology 2021Quote: ... The nitrocellulose strips were blocked in PBS with casein for 30 mins at room temperature and then probed for 1 hour with monoclonal antibodies (mAb) recognizing H3 HA (clone 36/2 (26)) or IAV M1 matrix protein (clone MCA401, BioRad). Strips were washed and incubated for 1 hour with a horse radish peroxidase (HRP)-conjugated secondary immunoglobulin (DAKO) ...
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bioRxiv - Microbiology 2020Quote: Supernatants of DMSO-and RAP-treated 1B4int schizonts samples following egress and invasion in Albumax-free RPMI 1640 medium were subjected to SDS-PAGE on 16.5% Mini-PROTEIN® Tris-Tricine gels (BioRad). Reduced and alkylated proteins from band slices excised from the entire separation profile were in-gel digested at 37°C overnight with 100 ng trypsin (modified sequencing grade ...
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bioRxiv - Biochemistry 2020Quote: Aliquots (250 μg total protein) of hippocampal extracts of WAR animals subjected to freeze/thaw were treated with 5 μL of dithiothreitol (Biorad) 50 μg/μL ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were homogenized using a handheld cell disruptor and sample concentrations were determined using a DC Lowry protein assay (BioRad). For cell lysates ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were incubated with secondary antibody for 1 h and proteins were revealed by ECL Prime (Amersham) using ChemiDoc Touch imager (Biorad).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Proteins migrated for 60 minutes at 200 V and were then transferred to a polyvinylidene fluoride membrane (Bio-Rad Laboratories) for 2 hours (70 V ...
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bioRxiv - Neuroscience 2020Quote: ... Results were normalized to the total amount of protein measured by the Bradford assay kit (Bio-Rad Laboratories, California, USA).
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bioRxiv - Neuroscience 2020Quote: ... Data were normalized to the total amount of protein measured by the Bradford assay kit (Bio-Rad Laboratories, California, USA).
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Checkpoint adaptation in repair-deficient cells drives aneuploidy and resistance to genotoxic agentsbioRxiv - Cell Biology 2019Quote: ... Proteins were visualized by chemiluminescence using ECL Western Blotting substrate (Pierce) on a ChemiDoc™ Touch imaging system (Bio-Rad). Alternatively ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were loaded on 4-12% Bis-Tris gels and run in 1X MOPS SDS Running Buffer in the Mini Gel Tank according to manufacturer’s Precision Plus Protein Dual Color Standards (Bio-Rad). Following electrophoresis ...
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bioRxiv - Synthetic Biology 2020Quote: ... Soluble and insoluble fractions were separated by centrifugation at 20,000 x g for 30 minutes and protein expression was screened by running both fractions on SDS-PAGE (Bio-Rad) and for selected samples also by negative stain EM ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were transferred onto PVDF membrane by wet western transfer using the Mini Trans-Blot® Cell Assembly (Bio-Rad) in buffer containing 25 mM Tris ...
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bioRxiv - Immunology 2021Quote: Concentration of 27 inflammation-related human proteins were measured in cell culture supernatants by using Human 27-plex BIORAD cytokine panel (Cat. No.171-A1112, BIORAD). Standard curves were generated for all 27 cytokine standards with eight 2-fold serial dilutions starting from 32 ng/ml ...
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bioRxiv - Cell Biology 2019Quote: ... proteins were transferred to a nitrocellulose membrane using the Trans-Blot Turbo transfer system (Bio-Rad Laboratories, Inc., Hercules, CA) for 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred onto a nitrocellulose membrane using the Trans-Blot® Turbo™ semi-liquid rapid transfer system (BioRad), according to the supplier’s recommendations ...
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bioRxiv - Biochemistry 2020Quote: ... Samples (1 µg total protein) were loaded onto non-reducing 12% Mini-PROTEAN® TGX™ precast gels (Bio-Rad), and molecular weight shifts and relative band intensity used to detect the presence and relative abundance of the crosslinked complex ...
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bioRxiv - Physiology 2019Quote: ... Protein lysates were prepared by centrifugation at 15,000g for 10min at 4°C and protein concentrations were calculated using Bradford assay (BioRad, UK) with BSA as a standard ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed directly in sample buffer and protein extracts were separated on a 4-20% polyacrylamide gel (Bio-Rad). Blotted nitrocellulose membranes were incubated with primary antibodies (mouse -p53 ...
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bioRxiv - Molecular Biology 2020Quote: ... the proteins were detected by scanning the gel for green (GFP) or red (mCherry) fluorescence using a ChemiDoc MP system (BioRad) and proteolytic cleavage was assessed from the changes in banding patterns ...
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bioRxiv - Genetics 2020Quote: ... TGX stain-free gels had total protein visualized prior to transfer and analyzed on Image Lab software (Image Lab 6.0, Bio-Rad) 41 ...
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bioRxiv - Cell Biology 2021Quote: Purified substrates and enzymes were centrifuged (16,000 g, 5 min, 4°C) before protein concentration was determined by Bradford assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: Tissue lysates (30 μg protein) were resolved by SDS–PAGE and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA). The membranes were incubated with antibodies against ROCK1 (Cat# ...
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bioRxiv - Physiology 2020Quote: ... and circulating factor concentrations were measured using the following kits according to the respective manufacturer’s protocols: Bio-Plex protein array system (Biorad, UK) with one multiplex panel was used to measure total Glucagon-like peptide-1 (GLP-1) ...
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bioRxiv - Neuroscience 2021Quote: ... 20 to 50 μg protein from each sample were resolved in 10% SDS-polyacrylamide gel and transferred onto 0.2 μm nitrocellulose membranes (Bio-Rad). 5% nonfat dry milk in Tris-buffered saline containing 0.1% Tween 20 was use to block the membranes ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μg of total protein/sample were loaded in each well of a 10% PAGE gel and transferred onto a nitrocellulose membrane (#1704158, BioRad) in a Trans-Blot Turbo system (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... ECM and RPE cell lysate (15-20 μg protein) were run separately on precast Mini-Protean TGX gels (Bio-Rad) and transferred onto PVDF membrane (Immobilon) ...
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bioRxiv - Neuroscience 2020Quote: ... 10μg/μl of protein was loaded into each lane of 4–15% (mg/100 mL) Tris·HCl polyacryamide gels (Bio-Rad). Protein was transferred to Immobilon-P PVDF membranes (Millipore) ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were separated in 10% SDS-polyacrylamide gels and electrotransferred to PVDF membranes using a semi-dry system (Bio-Rad). Biotin incorporation was assessed by incubating blots with streptavidin-HRP (1:1000 v/v ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were then transferred to a nitrocellulose membrane using a Mini Trans-Blot Cell Wet/Tank blotting system (BioRad). After transfer ...
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bioRxiv - Neuroscience 2021Quote: ... Samples (10 µg protein) were denatured at 95°C for 5 min in 4x Laemmli Sample Buffer (1610747, Bio-Rad) or Novex NuPAGE LDS Sample Buffer (NP0007 ...
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bioRxiv - Biochemistry 2021Quote: ... Ni-NTA and FLAG elutions were analyzed by SDS-PAGE on AnykD Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad). The samples were not heated before loading to preserve the fluorescence of the GFP and mCherry tags ...
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bioRxiv - Molecular Biology 2021Quote: ... protein bands were visualized via chemiluminescence (ECL Enhanced Chemiluminescence; Pierce ECL Western Blotting Substrate) using the ChemiDoc MP system (BioRad).
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bioRxiv - Developmental Biology 2021Quote: ... Loading was normalized to total protein for monoubiquityl-Histone H2A and Ubiquitin blots using AnyKD TGC Stain-Free gels (BioRad). Loading for monoubiquityl-Histone H2B blots was normalized to mouse anti-β-tubulin (1:1000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... The amount of ATP for one cell was derived from the amount of protein in one cell found via Bradford assay (BioRad).
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bioRxiv - Biochemistry 2021Quote: ... Protein samples were separated on 10 or 12% SDS-PAGE gels and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA) using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...