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Citations for Bio-Rad :
7051 - 7100 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 50-100mg of protein was loaded and resolved by SDS PAGE and transferred onto PVDF membrane (BioRad). The membrane was blocked using 5% BSA in TBST for 1 hour at room temperature and incubated with primary antibody overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... conjugated antibody was used to detect the desired protein by chemoluminescence with ECL (Bio-Rad, #170-5061).
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bioRxiv - Bioengineering 2024Quote: ... 30µg of protein was separated by electrophoresis through 7.5% polyacrylamide gels (Mini-PROTEAN, #456-8024, Bio-Rad) and transferred to nitrocellulose membranes (#1704159 ...
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bioRxiv - Neuroscience 2024Quote: ... Reagents for SDS-polyacrylamide gel electrophoresis and Bradford protein assays were from Bio-Rad (Hercules, CA, USA), fetal bovine serum and enhanced chemiluminescence (ECL ...
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bioRxiv - Cell Biology 2024Quote: ... and mixed with a one-third volume of 4×Laemmli protein sample buffer (cat. # 1610747, Bio-Rad). Following centrifugation at 20,000×g at 4°C for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... 10–20 μg of total protein was separated on 4–12% Bis-Tris gradient gels (Bio-Rad) by SDS-PAGE and then transferred to nitrocellulose membranes ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Proteins were transferred to a PVDF membrane using the Trans-Blot Turbo system (Bio-Rad Laboratories, USA). The membrane was washed with TBST solution and then blocked with Quick Blocking Buffer (NCM biotech ...
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bioRxiv - Molecular Biology 2024Quote: ... Thirty micrograms of protein lysate were resolved on 4-12% TGX Acrylamide Stain-Free gels (Bio-Rad). The Stain-Free gels were imaged prior to transfer to PVDF (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... and 15ug of each sample was loaded into a 4-20% SDS-PAGE precast protein gel (BioRad). Proteins were fractionated by electrophoresis and then transferred to PVDF membranes (BioRad) ...
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bioRxiv - Biophysics 2024Quote: ... 50 μg of protein was loaded in each well of a 4-20% precast polyacrylamide gel (BioRad). Gels were transferred (1.5 A for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Protein samples were prepared for western blot analysis by adding 4x sample buffer (Bio-Rad, Cat. #1610747) and 1 mM 2-mercaptoethanol (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... 15 µg of protein sample was run on precast 4-15% gradient Tris-Glycine gels (BioRad 4561084) at 90V for 15 minutes and 120V for 60 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Band densities were normalized to the total amount of protein loaded per lane using Sypro Ruby (BioRad).
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bioRxiv - Cancer Biology 2024Quote: ... proteins were transferred onto nitrocellulose membranes using Trans-Blot® TurboTM Transfer System RTA Transfer Kit (BioRad). Blotted membranes were blocked in 10% non-fat milk in PBS-Tween 20 0.05% for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Unconjugated dye was removed by passing the protein through Micro Bio-spin P-6 column (Bio-rad) equilibrated in GF150 buffer supplemented with 10% glycerol ...
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bioRxiv - Biochemistry 2024Quote: ... 30µg of protein was added per well and run on a 4-20% pre-cast gel (Biorad). Membranes were probed with α-ALDH2 (Proteintech 15310-1-AP ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysate (20 µg) was separated on 4-20% Mini-PROTEAN® TGX Stain-FreeTM protein gels (Biorad) and transferred onto Immun-Blot® Low Fluorescence PVDF membrane (Biorad) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10-20 mg protein per lane was electrophoresed on 4-15% precast gradient gels (Bio-Rad). Based on the protein concentration and sample volumes ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to PVDF using either a Trans-Blot Turbo system (with Turbo transfer buffer) (BioRad) or by traditional wet tank transfer using CAPS Buffer with 10% Methanol (for DNA-PKcs) ...
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bioRxiv - Immunology 2024Quote: ... Proteins were transferred to nitrocellulose membranes using a Trans-Blot® Turbo blot transfer pack (Bio-Rad). Nitrocellulose membranes were blocked with Odyssey blocking buffer (Li-COR Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... T3SS effectors/proteins from supernatant were resuspended in Urea 8M and mixed with Laemmli buffer (Bio-Rad). OD600 of bacterial cultures was used to normalize the amount of sample for loading into the SDS-PAGE.
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bioRxiv - Neuroscience 2024Quote: ... then proteins were separated by SDS-PAGE and transferred to a PVDF membrane (BIO-RAD, 162-0177). The membrane was blocked with 5% skim milk in PBS containing 0.05% Tween 20 and then incubated with rabbit anti-RhoA (1:1000 ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were transferred to a polyvinylidene difluoride membrane using the Trans-Blot Turbo transfer system (Bio-Rad). The membranes were blocked with 5% BSA in tris-buffered saline - 0.1% Tween (TBST ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Samples were resolved on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, 4568096) or 12% polyacrylamide gels and transferred to nitrocellulose membranes ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Bait and prey proteins were loaded on separate gels (Bio-Rad 4-20% precast gels cat. #4568094). Proteins were transferred to nitrocellulose membrane and immunoblotted as described above ...
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bioRxiv - Plant Biology 2024Quote: ... The Supernatant was isolated and sample protein concentrations were calculated by Bradford assay (Bio-Rad Laboratories, 2010) using the Quick Start Bradford 1x Dye Reagent (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... 20 μg of protein lysate was loaded onto an SDS-polyacrylamide gel and subjected to electrophoresis (BioRad). The proteins were then transferred from the gel onto a nitrocellulose membrane using a semi-dry method (BioRad Trans-Blot Turbo) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were run on 4-15% Mini-PROTEAN TGX precast protein gels (Bio-Rad Laboratories, Hercules, California) in running buffer (250 mM Tris ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The specific protein bands were visualized with a secondary antibody (anti-rabbit IgG-HRP conjugate; Bio-Rad) using a chemiluminescent substrate (Amersham ECL Western Blotting Detection Reagents ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred onto 0.2 μm nitrocellulose membrane (Bio-Rad, Hercules, CA). After blocking with 5% skimmed milk ...
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bioRxiv - Cancer Biology 2021Quote: ... 30 μg of denatured proteins were separated by SDS-PAGE (Mini-PROTEAN TGX Gels 10%, Bio-Rad Laboratories) and transferred to 0.45 μm nitrocellulose membranes ...
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bioRxiv - Cell Biology 2020Quote: ... Seventy-five micrograms of protein lysate were resolved on 4-12% TGX Acrylamide Stain-Free gels (Bio-Rad). Stain-Free gels were imaged prior to transfer to PVDF membrane (Millipore) ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were separated by SDS-PAGE and transferred to nitrocellulose using a Trans-blot Turbo system (Bio-Rad). Protein concentrations were measured by Bradford assay using Protein Assay Dye Reagent Concentrate (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations in the cell lysates were estimated using the Quick Start™ Bradford Dye Reagent (Biorad# 5000205) followed by blocking of free thiol groups in HEN buffer containing 0.2% MMTS and 2.5% SDS at 50°C for 30 minutes ...
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bioRxiv - Biophysics 2020Quote: ... The cleared lysates were first run on precast protein gels (PN:456-1096 or 456-1093, Bio-Rad) in MOPS SDS running buffer (B0001 ...
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bioRxiv - Microbiology 2021Quote: ... 10-15 µg of protein lysate was resolved by SDS-PAGE on TGX Stain-Free acrylamide gels (BioRad). Membranes were blocked in 5% BSA or 5% skim milk in Tris buffered saline-Tween20 (TBS-T) ...
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bioRxiv - Genomics 2021Quote: ... The proteins were separated in the first dimension on 4-7 and 5-8 IPG strips (Bio-Rad). The strips were then loaded onto 8-20% gradient PAGE for separation on the second dimension ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein concentration was determined and 150 or 300 μg was loaded onto precast 4-15% TGX gels (BioRad) and blotted onto PVDF membranes for 1 hour at 100V at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... and 20 μL diluted protein was added to each well of a 96-well PCR plate (Bio-Rad) in triplicate ...
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bioRxiv - Developmental Biology 2020Quote: ... Equal amounts of protein were loaded onto 10% or 4–15% Mini-PROTEAN TGX precast gels (Bio-Rad) and analyzed by Western blot ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein extract equivalent to 50 μg was loaded per lane onto precast gradient gels (4-20% Bio-rad). Separated proteins were then transferred to nitrocellulose membranes ...
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bioRxiv - Physiology 2022Quote: ... 15µl sample mix containing 12µg total protein and 4µl sample buffer (4× Laemmli Sample Buffer, Bio-Rad, 1610747) with 10% mercaptoethanol (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein samples were subjected to SDS-β PAGE gel electrophoresis using pre-made gradient gels (4-20%, Biorad). Proteins were transferred by wet transfer to a PVDF membrane (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... 10-15 µg of protein lysate was resolved by SDS-PAGE on TGX Stain-Free acrylamide gels (BioRad). Total protein images were acquired from the PVDF membranes after transfer on the ChemiDoc Touch Imaging system (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... Total protein images were acquired from the PVDF membranes after transfer on the ChemiDoc Touch Imaging system (BioRad). Membranes were blocked in 5% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2022Quote: ... 10-15 µg of protein lysate was resolved by SDS-PAGE on TGX Stain-Free acrylamide gels (BioRad). Total protein images were acquired from the PVDF membranes after transfer on the ChemiDoc Touch Imaging system (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... Total protein images were acquired from the PVDF membranes after transfer on the ChemiDoc Touch Imaging system (BioRad). Membranes were blocked in 5% BSA in TBS-T (Tris-buffered saline 0.1% Tween-20) ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then measured to 20μg total protein in 2x Laemmli buffer containing 10% b-mercaptoethanol (Bio-rad), heated at 70°C for 10min and loaded into 4-20% precast gels (Bio-rad ...
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bioRxiv - Neuroscience 2020Quote: ... Protein samples were prepared to a final concentration of 5 μg/μl in 4x Laemmli buffer (Bio-Rad) containing 25 mM DTT ...
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bioRxiv - Molecular Biology 2020Quote: ... Calcium levels were normalized to protein levels measured using the Bradford assay (Bio-Rad Laboratories, Hercules, CA, # 5000006).