Labshake search
Citations for Bio-Rad :
6851 - 6900 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... the resin-bind proteins were eluted and separated by Econo-Column® Chromatography Columns (BioRad, Cat# 7371512). The protein concentration of eight collected fractions was measured and the three protein-enriched fractions were pooled and dialyzed successively in Dialysis Buffer and Storage Buffer ...
-
bioRxiv - Cell Biology 2021Quote: ... sample aliquots were used for protein separation on 8-16% TGX-Criterion gels (Bio-Rad, Hercules, CA). After electrophoresis ...
-
bioRxiv - Microbiology 2020Quote: ... The target proteins were visualized following the protocol supplied with Clarity™ Western ECL (Bio-Rad, USA) or with the ONE-HOUR Western™ Standard Kit (Genscript ...
-
bioRxiv - Cell Biology 2021Quote: ... overnight following with 15 μL prewashed protein A/G beads (1:1 mixture, 1614013 and 1614023, Biorad) incubation for another 1.5 hr ...
-
bioRxiv - Microbiology 2021Quote: ... Fifteen μg of total protein was loaded onto a 4–20% Mini-PROTEAN TGX gel (Bio-Rad), then transferred to a polyvinylidene difluoride (PVDF ...
-
bioRxiv - Neuroscience 2021Quote: ... WCL were then cleared by centrifugation at 13,000 × g and assayed for total protein (Bradford assay, Biorad). Biotinylated lysates were equalised to between 0.5 and 1 mg/ml total protein concentration ...
-
bioRxiv - Developmental Biology 2021Quote: ... SDS PAGE was performed using 7.5% Mini-PROTEAN® TGX™ precast protein gels (BIO-RAD, 4561023) run in running buffer (25mM Tris ...
-
bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to a Hybond ECL membrane (Amersham biosciences) using a semi-dry transfer system (Biorad) and probed with primary antibodies ...
-
bioRxiv - Immunology 2020Quote: ... Proteins were separated by SDS-PAGE using 4-20% pre-cast Mini-Protean TGX gels (Bio-rad) before transfer onto a nitrocellulose membrane using the Trans-Blot turbo transfer system (Bio-rad) ...
-
bioRxiv - Physiology 2021Quote: ... 20 μg of protein were loaded for separation in 10% acrylamide SDS-PAGE gels (161-0182, Biorad) before transfer on PVDF membrane (Trans-Blot Turbo ...
-
bioRxiv - Neuroscience 2021Quote: ... Aliquots of protein were boiled at 95°C in 2X Lamelli buffer (Bio-Rad, Hercules, CA, USA) for 5 min ...
-
bioRxiv - Biochemistry 2021Quote: ... 24 µg of proteins were separated on SDS-PAGE gels (BioRad 4-15% TGX pre-cast gels), followed by transfer onto Protran 0.45 µm nitrocellulose membranes (GE Healthcare ...
-
bioRxiv - Developmental Biology 2021Quote: ... SDS-PAGE was performed using Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad), loading 10 embryo caps per lane ...
-
bioRxiv - Developmental Biology 2022Quote: ... total protein extracted from dechorionated and deyolked embryos was resolved on 12% gels (Bio-Rad Laboratories, 1610175) and then transferred to the PVDF membrane (Merck Millipore ...
-
bioRxiv - Cell Biology 2021Quote: ... Twenty micrograms of protein were resolved on a 4-12% Bis-Tris gel (Bio-Rad, Hertfordshire, UK) and transferred to nitrocellulose membranes ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Supernatant protein concentration was determined through a commercially available Bradford Assay kit (Bio-Rad Laboratories, CA, USA). Equal quantities (50µg ...
-
α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... The total protein samples were separated using by 12% SDS-PAGE and transferred onto nitrocellulose membranes (BioRad). The membranes were blocked using 5% milk and incubated with primary antibodies for α-catenin (1:1000 ...
-
bioRxiv - Pathology 2021Quote: ... Proteins were transferred to polyvinylidene difluoride (PVDF) membranes with a Trans-Blot Turbo Transfer System (Bio-Rad). IL-6 downstream signaling activation was monitored using primary antibodies against STAT3 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Equal protein loads of 8ug were resolved on precast 8% to 16% Bis-Tris gels (Bio-Rad) under reducing conditions ...
-
bioRxiv - Cell Biology 2020Quote: ... proteins separated by SDS-PAGE were transferred on to Immun-Blot PVDF membrane (Bio-Rad, Hercules, CA). The blotted membranes were blocked with 0.3% skim milk in TTBS (50 mM Tris ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein samples were loaded and separated on a 4-15% Mini-PROTEAN-TGX Gel (#456-1084, BioRad) along with 5µL of protein ladder (Full-Range RPN800E ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were resolved via SDS-PAGE in 10% Mini-PROTEAN Tris-Glycine gels (Bio-Rad, Hercules, CA) and wet transferred to 0.45 μm nitrocellulose membrane (GE Healthcare) ...
-
bioRxiv - Biochemistry 2021Quote: ... The total pellets were dried and their protein concentrations were determined using a Bradford assay (Bio-Rad). The proteins were reduced and alkylated in the buffer containing 100 mM triethyl ammonium bicarbonate (TEAB ...
-
bioRxiv - Genomics 2021Quote: ... whole-cell extract in the supernatant was measured using the Bio-Rad Protein Assay (Bio-Rad, 5000006). 15 μg of protein extracts were mixed with NuPAGE LDS Sample Buffer (Invitrogen ...
-
bioRxiv - Microbiology 2020Quote: ... Protein samples were separate in 10% SDS-polyacrylamide gel using the Miniprotean II electrophoresis system (Bio-Rad). The gel was stained with a fixative solution of 40% MeOH ...
-
bioRxiv - Immunology 2020Quote: ... and then proteins were transferred to Trans-Blot® Turbo Midi PVDF membranes (Bio-Rad; Hercules, CA) using the Trans-Blot® Turbo™ Transfer System (Bio-Rad ...
-
bioRxiv - Biophysics 2021Quote: ... and quantifying the band intensities corresponding to the target protein using the ImageLab software from Bio-Rad.
-
bioRxiv - Neuroscience 2021Quote: ... and 30 μg of protein in Laemmli sample buffer was loaded onto 10% polyacrylamide gels (Bio-Rad). After SDS-PAGE ...
-
bioRxiv - Neuroscience 2021Quote: ... The protein content of the CME was determined using the Pierce BCA assay (#5000001; BioRad, Watford, UK) and the content of myelin-derived axon growth inhibitory molecules such as myelin basic protein (#MCA409S ...
-
bioRxiv - Immunology 2022Quote: ... 10-30 ug total protein were resolved by SDS-PAGE and transferred to PVDF membranes (Bio-Rad). Primary antibody incubations were done overnight with antibodies diluted in 3% BSA in TBS-T (Tris-buffered saline/Tween 20) ...
-
bioRxiv - Immunology 2022Quote: ... were used to separate proteins that were transferred on Transblot Turbo polyvinylidene fluoride membranes (Bio-Rad laboratories) with Transblot Turbo Transfer System ...
-
bioRxiv - Cancer Biology 2022Quote: ... A total of 30 µg of protein per sample were diluted in Laemmli Sample Buffer (Bio-Rad) and run for 50 min at 120V into a Mini-PROTEAN® TGX Precast Gel ...
-
bioRxiv - Molecular Biology 2022Quote: ... Protein was transferred to a pre-activated PVDF membrane using Trans-Blot Turbo Transfer System (Bio-Rad). The blots were incubated with the following primary antibodies overnight at 4 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred to polyvinylidene difluoride (PVDF) membranes with the Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked 10 min with 8% (w/v ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein concentration in the collected lysates was determined using the DC Reagent Assay kit from Bio-Rad as per the manufacturer’s protocol (Cat ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein samples were transferred to a methanol pre-activated PVDF membrane (Bio-Rad, Cat#1620177, Bio-Rad) by running them in transfer buffer (TBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... Protein concentration of the lysates was measured using Quick Strat Bradford assay (5000205, Bio-Rad, Hercules, CA). Lysates (32 μg proteins/lane ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein samples were transferred to a methanol pre-activated PVDF membrane (Bio-Rad, Cat#1620177, Bio-Rad) by running them in transfer buffer (TBS ...
-
bioRxiv - Neuroscience 2022Quote: ... 40 μg of proteins were mixed with the right amount of Laemmli Sample Buffer 4X (Bio-Rad), and boiled ...
-
bioRxiv - Biochemistry 2022Quote: ... The molecular mass of proteins was calibrated using Precision Plus Pre-stained Standards markers from Bio-Rad Laboratories (Hercules ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein samples were subjected to 12 % SDS-polyacrylamide gel electrophoresis (PAGE) and transferred to nitrocellulose membranes (BioRad). Membranes were blocked for 30 min at room temperature in Tris-buffered saline supplemented with 0.05 % Tween 20 (TBS-T ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred to polyvinylidene difluoride (PVDF) membranes (Trans-Blot®Turbo™LF PVDF, Bio-Rad) followed by blocking in 2% BSA and primary antibody incubation in 5% fat-free milk powder in Tris-buffered saline with 0.1% Tween-20 overnight at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: ... Equal amounts of proteins were resolved using 4-20% SDS-PAGE (Bio-Rad, cat. no: 456-1094), and blotted onto 0.2 μm PVDF membranes (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred to polyvinylidene difluoride (PVDF) membranes (Trans-Blot® Turbo™ LF PVDF, Bio-Rad) followed by blocking in 5% fat-free milk powder and antibody incubation in 5% fat-free milk powder in Tris-buffered saline with 0.1% Tween-20 ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples were loaded into 4–20% Mini-PROTEAN® TGX™ Precast Protein gels (#4561093, Bio-Rad Laboratories ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred to a nitrocellulose membrane using the Mini Trans-Blot® Cell (1703930, Bio-Rad) system by blotting in tris-glycine based buffer (25 mM Tris ...
-
bioRxiv - Cell Biology 2022Quote: ... 30-50 µg of protein per lane were separated on CRITERION 4-12% SDS polyacrylamide gels (Biorad) and transferred to 0.2 µm nitrocellulose membrane (GE Healthcare ...
-
bioRxiv - Genomics 2022Quote: ... Lysed proteins were resolved by SDS-PAGE using Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad). Primary antibodies used include ...
-
bioRxiv - Genetics 2022Quote: ... Total protein on the membrane was visualized as stain-free signal using ChemiDoc MP Imagers (Bio-Rad). Immunoblotting images were captured using Odyssey Infrared Imaging system with application software V3.0.30 (LI-COR ...
-
bioRxiv - Genomics 2022Quote: ... 20 µg total protein extract per sample were boiled in final 1 x Laemmli Buffer (BioRad, 1610747) containing 10% 2-Mercaptoethanol (M6250 ...