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Citations for Bio-Rad :
6901 - 6950 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... The proteins were transferred onto a nitrocellulose membrane using a semi-dry system (BioRad, Hercules, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Twenty-three µg of protein were applied to each lane of a gradient gel (7.5%; Bio-Rad). Proteins were separated by electrophoresis at a constant 130 V/gel for 90 min and transferred to a nitrocellulose membrane on the Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... and concentrations were determined using the Bio-Rad protein assay with BSA as a standard (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: ... a small aliquot of each sample was used for protein measurement using the Bradford assay (Bio-Rad). Samples were then concentrated down to 20 µL and supplemented with 5 µg of sequencing grade trypsin (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... 15ug of total proteins/sample was loaded in a precast 4-15% polyacrylamide gel (Bio-Rad #4561033EDU) and run at 100mV for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... For each sample an equal mass of proteins was resolved on 4–15 % polyacrylamide gel (Bio-Rad). The proteins were then transferred (300 mA ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were transferred to PVDF membranes using Trans-Blot Turbo RTA Mini transfer kit (Bio-Rad, #1704727). Membranes were blocked with 5% milk in 1x TBST for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred onto a nitrocellulose membrane using the Trans-Blot Turbo Transfer system (BioRad, Cat. #1704150) and membranes were blocked for 1 h in 5% milk at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... A total 20 μg of protein lysates was separated by 4-12% SDS-PAGE (Bio-Rad, 4561095) and transferred to a nitrocellulose membrane ...
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bioRxiv - Neuroscience 2022Quote: ... The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG ...
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bioRxiv - Neuroscience 2022Quote: ... pH 7.4 and protease inhibitors) and quantified using the BCA protein determination method (Bio-Rad, Hercules, CA). Protein samples were diluted to equivalent volumes containing 20 μg of protein and boiled in an equal volume of Laemli SDS boiling buffer (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... proteins in the gels were transferred to 0.2 µm nitrocellulose membranes using the TransBlot TURBO system (BioRad). Membranes were blocked in 5% non-fat milk in TBS-T on an orbital shaker overnight at 4°C on a rocker ...
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bioRxiv - Neuroscience 2022Quote: ... samples were loaded on 4-20% gradient mini-PROTEAN® TGX™ precast protein gels (Bio-Rad, 10-well Cat no ...
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bioRxiv - Neuroscience 2022Quote: ... next to the Precision Plus Protein Dual Color Standard (catalog#161-0374, Bio-Rad, Hercules, CA, USA) in SDS-PAGE running buffer (catalog#1610788 ...
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bioRxiv - Cell Biology 2022Quote: ... Run was performed in a commercial 10% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) at 35mA ...
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bioRxiv - Plant Biology 2022Quote: ... The Superdex 200 10/300 GL column was calibrated with the gel filtration protein standard (Bio-Rad).
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bioRxiv - Physiology 2022Quote: ... Approximate molecular masses were determined by comparison with the migration of pre-stained protein standards (Bio-Rad). Quantitative estimation of the bands’ intensity was performed using ImageJ software (U.S ...
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bioRxiv - Microbiology 2022Quote: ... Twenty-three µg of protein were applied to each lane of a gradient gel (7.5%; Bio-Rad). Proteins were separated by electrophoresis at a constant 130 V/gel for 90 min and transferred to a nitrocellulose membrane on the Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then run on a 4-15% Tris-glycine protein gel (Bio-Rad; Hercules, CA, USA) at 150V until separated ...
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bioRxiv - Molecular Biology 2024Quote: ... equal proteins loads were separated and then transferred to Polyvinylidene difluoride membrane (PVDF) (Bio-Rad, Cat# 1704156). Afterward ...
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bioRxiv - Cell Biology 2023Quote: ... and proteins (25μg/lane) were resolved on 4-20% Criterion TGX Stain Free gels (Bio-Rad Laboratories) by SDS/PAGE ...
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bioRxiv - Bioengineering 2023Quote: ... and 20-30 μg total protein were run in Mini-PROTEAN TGX Precast gels (#4561033, Bio-Rad). Transfer to a nitrocellulose membrane was done using 25 V for 6 min in the iBlot2 Gel Transfer device (IB21001 ...
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bioRxiv - Biochemistry 2024Quote: ... Afterwards proteins were transferred onto nitrocellulose membranes using the Trans-Blot Turbo transfer system (BioRad, 1704270, 1704159). Nitrocellulose membranes were blocked at RT in blocking solution containing 5% milk powder in TBST (20mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... and the protein bands were detected and visualised using a transilluminator kit (Bio-Rad, Hercules, CA, USA). The intensities of the bands were quantified by scanning densitometry (Un-SCAN software ...
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bioRxiv - Biochemistry 2024Quote: ... The measurements were normalized by calculating the total protein amount in each well using Bradford reagent (Biorad).
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bioRxiv - Biochemistry 2024Quote: ... Lysates (20-40 μg) were run on SDS-PAGE gels with Precision All-Blue Protein Standards (BioRad) before being transferred onto nitrocellulose membranes ...
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bioRxiv - Biophysics 2024Quote: The protein concentration from U87 or T98G cell lysates was determined using Bradford Assay reagent (Bio-Rad). The lysates were normalized based on total protein concentration ...
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bioRxiv - Cancer Biology 2024Quote: ... The proteins and ladder were then imaged using the ChemiDoc MP imaging system (Bio-Rad Laboratories, Inc.) using the autoexposure and chemiluminescence to observe the bands and 635 nm of light with autoexposure for visualization of the ladder.
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bioRxiv - Cancer Biology 2024Quote: ... was used to transfer proteins from gel to the LF-PVDF membrane (Bio-Rad, cat. no: 1704274). For immunoblotting ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were electro-transferred into nitrocellulose membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) and blocked overnight at 4°C in blocking buffer (5% milk powder (w/v) ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples were subsequently separated on a 4-20 % gradient Mini Protean TGX Precast Gel (BIO-RAD). Whole cell lysate samples were all loaded with the same volume ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of protein (30 μg) from each sample were loaded into SDS-PAGE gels (BIO-RAD) for electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... 40 µg of sample protein was diluted with NP-40 buffer and SDS Sample Buffer (Bio-Rad) and were then loaded into 10% precast gels (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... The denatured protein samples were subjected to electrophoresis using a 4-20% SDS-PAGE gel (Bio-Rad) in running buffer and subsequently transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2024Quote: ... The separated proteins were transferred onto nitrocellulose membranes using the Trans-Blot Turbo transfer system (Bio-Rad). To block nonspecific binding ...
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bioRxiv - Cell Biology 2024Quote: ... Soluble proteins were subject to immunoblot analysis using 4-15 % SDS-PAGE gels (Bio-Rad, Cat. # 64557025) followed by transfer to PVDF membrane ...
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bioRxiv - Cell Biology 2024Quote: ... The separated proteins were transferred to a nitrocellulose membrane using a Mini Trans-blot Module (Bio-Rad) at constant 100V for 60-90 min ...
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bioRxiv - Immunology 2024Quote: ... 20μg of proteins were separated by electrophoresis on any KD (Kilo Dalton) (Bio-Rad, Cat no. 4569033) SDS-polyacrylamide gel ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to Trans-Blot Turbo 0.2 µm Nitrocellulose Transfer Membrane (Bio-Rad, Cat#1704158/1704159) and membranes blocked with 5% non-fat milk for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... Eluted proteins were buffer-exchanged into Tris pH 7.4 using Micro Bio-Spin chromatography columns (Bio-Rad) and concentrated using Amicon Ultra 0.5 mL centrifugal filters with a 3 kD cutoff.
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bioRxiv - Microbiology 2023Quote: ... Concentrated virus was then inactivated and proteins denatured by heating with 4x Laemmli sample buffer (Bio-Rad) with 10% β-mercaptoethanol and subsequently run on a 4-20% Mini-PROTEAN TGX protein gel (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were separated by SDS PAGE using Mini-Protean TGX stain-free 4-20 % precast gels (BioRad).
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bioRxiv - Cancer Biology 2024Quote: EV protein aliquots were lysed by boiling with 20% β-mercaptoethanol in 4X Laemmli buffer (Bio-Rad) for 5 min at 100°C ...
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Treatment with furosemide indirectly increases inhibitory transmission in the developing hippocampusbioRxiv - Neuroscience 2023Quote: ... For each sample an equal mass of proteins was resolved on 4–15 % polyacrylamide gel (Bio-Rad). The proteins were then transferred (300 mA ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were then transferred to a PVDF membrane using a Trans-Blot Turbo Transfer System (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Individual oxidized proteins were resolved by SDS-PAGE on 4-20% polyacrylamide gel gradient gel (BioRad, 5671093). Resolved proteins were transferred to PVDF membrane at 75 V for 2h ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by SDS-PAGE on 4-15% gradient gels (Mini-PROTEAN Precast gels, Bio-Rad) in running buffer (200 mM glycine ...
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bioRxiv - Genetics 2023Quote: Samples were electrophoresed on 4 to 15% Mini-PROTEAN TGX Stain-Free protein gels (Bio-Rad, #4568084) for 60 min at 100 V ...
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bioRxiv - Genetics 2023Quote: ... Low-level expressed gene ribosomal protein S15 (RPS15) was preamplified with SsoAdvanced PreAmp Supermix (#1725160, Bio-Rad), with 3 min at 95°C ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were transferred to new tubes and total protein was quantified using a Bradford Assay (Bio-Rad). 20 µg protein per sample was reduced with 3 mM TCEP (Sigma ...