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Citations for Bio-Rad :
601 - 650 of 1046 citations for Lupatadine fumarate EP Impurity C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... were added to the assembled PURE and incubated for 4h at 37 °C in a PCR thermocycler (Bio-Rad, USA). The reactions were incubated for 2 minutes at 60 °C and assessed with SDS-PAGE (160V 1h ...
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bioRxiv - Cell Biology 2024Quote: ... Protein samples were boiled for 10 min at 95°C in 1X Laemmli buffer (LB) with β-Mercaptoethanol (BioRad, 1610747).
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bioRxiv - Cancer Biology 2024Quote: ... overnight at 4°C washed in TBST (3×10 min at RT) and then probed with the corresponding peroxidase-conjugated secondary antibody (Biorad) for 1h at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... sections were incubated overnight at 4°C with anti-F4/80 antibody (F4/80 antibody | Cl:A3-1, Bio-Rad, USA). Endogenous peroxidases were quenched by incubating the sections in 3% hydrogen peroxide (Acros Organics ...
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bioRxiv - Cancer Biology 2024Quote: ... and a final extension at 68 °C for five minutes using C1000 TOUCH CYCLER w/48W FS RM (Bio-Rad). PCR products were resolved on 2% agarose gel and visualized with Nancy-520 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... The qRT-PCR reactions were performed using the CFX96 Real-Time PCR System [C-1000 Touch Thermal Cycler] (BIO-RAD) and relative gene expressions were calculated using the ΔΔCT method normalized to S18 housekeeping gene ...
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bioRxiv - Genetics 2024Quote: ... followed by incubation at 65°C for 10:00 and 72° for 10:00 (note: exact temperature settings for the modified BioRad T-1000 thermocycler included with the iCell8 were based on the conversion tables in Appendix G ...
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bioRxiv - Cell Biology 2024Quote: ... the solution was boiled for 2 x 7 min at 98°C and filtered through a 0.2 pm cellulose acetate membrane (Bio-Rad) with a 96-well vacuum Bio-Dot Apparatus (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... One or two micrograms of the concentrated viruses were heated at 95 °C for 5 min before being resolved on 4-20% SDS-PAGE (Bio-Rad) using the Novex™ Sharp Pre-stained Protein Standard (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants were mixed with SDS sample buffer containing DTT and incubated for 5 min at 95°C prior to fractionation by SDS-PAGE analysis on 4%–15% Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Transfer to nitrocellulose membranes and probing for Western blot analysis was as described previously ...
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bioRxiv - Evolutionary Biology 2020Quote: Samples were heated at 95°C for 10 minutes before loading into 7.5% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) and running at 150V for approximately 90 minutes at 4°C in 1X tris-gylcine running buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... which is pre-equilibrated by Buffer A before use with rotation at 3 hr in 4°C in Econo-Pac Chromatography Columns (20 mL, Bio-Rad). After the unbounded fraction was discarded ...
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bioRxiv - Cancer Biology 2021Quote: ... diluted in nuclease-free water and the primer(s) were incubated at 65°C for 5 minutes in the DNA Engine Dyad®Peltier Thermal Cycler (BioRad). Afterward ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... Protein samples were heated at 55°C for 10 min and 10 μl was loaded on SDS–PAGE (4-20% Mini-PROTEAN TGX Stain-Free, Bio-Rad) and analyzed by Western blot.
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bioRxiv - Evolutionary Biology 2021Quote: ... to 10 μL RNA and incubating at 65°C for 5 min on a C1000 Touch Thermal Cycler (Bio-Rad, USA) followed by chilling on ice ...
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bioRxiv - Biochemistry 2022Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Neuroscience 2021Quote: ... was then added and the solution was mixed at 4°C for 10 minutes before addition of 100 mg of Biobeads SM2 (Bio-Rad). Biobeads were washed into methanol ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleavage-related change in fluorescence signal of the probe was measured every 30 s for 60 min under incubation at 37°C using a Real-time PCR system (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were heated to 50°C for 1-10 min before addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594), vortexing vigorously for 5 s before pipetting in plug mould (Bio-Rad 1703713 ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were heated to 50°C for 2-3 min before addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594), vortexing vigorously for 5 s before pipetting in plug mould (Bio-Rad 1703713 ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were fully resuspended by vortexing and incubated at 55°C for 15 minutes in a C1000 Touch thermal cycler (BIO-RAD) then re-pelleted with the magnet ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μl of the cell suspension was spread on a single partitioned YPDA plate and incubated at 30°C for 48 h prior to imaging on a ChemiDoc imaging system (Bio-Rad). The area of all colonies ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were cleared by centrifugation at 16000xg for 5 min at 4°C and the total protein concentration in the lysates was measured by Bradford protein assay (Bio-Rad). For western blot analysis 25 µg of each lysate was boiled for 5 min at 98°C (for the detection of LC3B and ATG9A samples were heated at 60°C for 10 min ...
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bioRxiv - Biophysics 2021Quote: ... The mixture was diluted to ~30 mL with sample buffer and stirred at 4°C for 30 min prior removal of C12E8 by adding 4 g of Bio-Beads SM-2 (Bio-Rad) in stages of 0.5 ...
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bioRxiv - Plant Biology 2020Quote: ... Liposomes were eluted from the beads by boiling at 95°C for five minutes after the addition of 10x sample reducing agent (Nupage, NP0009) and 4x Laemmli sample buffer (Biorad, #1610747).
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bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary antibodies (Supplementary Table 1) overnight at 4°C and visualised with HRP-conjugated anti-IgG secondary antibodies and enhanced chemiluminescence substrates (Bio-Rad). The expression of PDHX or calnexin was used as a loading control.
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bioRxiv - Biochemistry 2021Quote: ... The melting curves were measured at 1°C/min at the FRET channel in triplicate measurements and the data analyzed with CFX Manager™ (BioRad).
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bioRxiv - Biochemistry 2021Quote: ... 5 μg of lysate was heat denatured for 3 minutes at 95°C for 3 minutes and resolved on a pre-cast 4-15 % SDS-PAGE gels (Bio-Rad) at 200V for 45 minutes in 1X running buffer (25mM Tris pH 8.3 ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Protein was eluted from beads by heating for 5 min at 95 °C in 1X Laemmli Buffer (Bio-Rad, Hercules, CA). Retrieved protein was detected by standard immunoblotting techniques using the following antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was incubated for 90 minutes at 4°C before the resin was collected in a 20 mL disposable column (Bio-Rad) and washed with 100 mL Buffer B + 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was incubated for 90 minutes at 4°C before the resin was collected in a 20 mL disposable column (Bio-Rad) and washed with 100 mL Buffer D ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was incubated for 90 minutes at 4°C before the resin was collected in a 20 mL disposable column (Bio-Rad) and washed with 100 mL Buffer C + 400 mM NaCl ...
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bioRxiv - Pathology 2021Quote: ... maximum power with 30 second intervals and the lysate obtained by ultracentrifugation at 100,000 g for 60 minutes, 4°C (CS 100FNX, HITACHI) resuspended in PBS and the concentration was measured by Bio-Rad protein assay dye reagent concentrate (BioRad) ...
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bioRxiv - Neuroscience 2021Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4°C and subsequently washed in TBST and incubated for 1 hr at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 72°C for 30 sec and followed by a melting curve) was performed in a CFX Real-Time Detection System (Bio-Rad) and relative expression calculated using the CFX Manager Software (Bio-Rad).
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bioRxiv - Plant Biology 2021Quote: ... and incubated on a rotary shaker for 60 min at 4°C before transfer to a Poly-Prep Chromatography Column (Bio-Rad). The column bed was washed with 40 ml of a wash buffer containing 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... The melting curves were measured at 1°C/min at the FRET channel and the data analyzed with CFX Manager™ (BioRad).
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bioRxiv - Bioengineering 2021Quote: ... 15 µg of the extracted protein samples were boiled for 5 minutes at 95 °C in 4X laemlli buffer (BioRad; 1610747), run on mini-PROTEAN TGX precast gels (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... according to their molecular weight and transferred to a nitrocellulose Hybond-C membrane using an iBlot Gel Transfer Device (Bio-Rad) or using a wet-transfer system (if protein molecular weight >120kDa) ...
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bioRxiv - Plant Biology 2020Quote: ... Plates were incubated for 30 °C for 5 or 7 days and imaged using a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... Clarified lysates were incubated with 2 ml of glutathione resin and rotated for 1 h at 4°C in a 25 ml disposable drip column (Bio-Rad). Settled resin was washed with 25 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... RNA samples were stored at −80°C until cDNA synthesis using the iScript™ gDNA Clear cDNA Synthesis Kit according to the manufacture’s guidelines (BIO-RAD). A qPCR-based assay was used to detect and quantify PfCSP transcripts (Kefi et al. ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were heated from 20 to 95 °C at the rate of 1 °C min-1 and fluorescence signals were monitored using quantitative real-time PCR (CFX96 Real-Time System, Bio-Rad). Each experiment was done in triplicate and the average values were taken for analysis ...
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bioRxiv - Microbiology 2020Quote: ... Proteins that bound to PI(3)P or control beads were eluted by boiling at 90–95 °C for 20 min in 70 μL 4x Laemmli sample buffer (Bio-Rad) and centrifuging at 20,000 g for 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was collected after centrifugation at 15,000 ×g for 15 min at 4°C and applied to a 20 mL open column (Poly-Prep Chromatography Columns; BIO-RAD) containing 500 µL of Ni-NTA agarose (Qiagen ...
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bioRxiv - Biophysics 2021Quote: ... fPanx1ΔC purity was assessed by SDS–polyacrylamide gel electrophoresis (PAGE) using 4-20% pre-cast gradient Tris-glycine gel (Bio-Rad) stained with Simply Blue (SimpleBlue Safe Stain ...
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bioRxiv - Cell Biology 2022Quote: ... lysates were then clarified through centrifugation (14’000 g, 20 minutes, 4°C) and then quantified using Bradford Reagent (Bio-Rad Laboratories) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... The samples were separated on a pre-heated 45 cm sequencing gel (45 °C, 65 W, 1 h) and visualized using a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: ... 5’-ATG GGT TCG CAT GGT CCT AAT GAC ACA C-3’) (Cousineau et al., 2022) and the GAPDH loading control (PrimePCR Probe assay with HEX probe, Bio-Rad). Each 20 µL reaction contained 500 ng of total RNA ...
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bioRxiv - Plant Biology 2022Quote: ... The extract was centrifuged at 18,000 g for 10 minutes at 4 °C and the protein content of the supernatant determined using the Bradford method (Bio-Rad) in a spectrophotometer measuring the absorbance at 595 nm and then eluted in Laemmli 2X buffer with 8 % β-mercaptoethanol (95 °C for 5 minutes) ...