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Citations for Bio-Rad :
701 - 750 of 1046 citations for Lupatadine fumarate EP Impurity C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... FLAG immunoprecipitates were eluted at 95°C for 10 minutes and GFP immunoprecipitates were eluted at 60°C for 20 minutes into 1 X SDS-Page Buffer (Bio-Rad) supplemented with 5% BME ...
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bioRxiv - Biochemistry 2022Quote: ... in denaturing conditions after incubation into XT-sample buffer (Bio Rad) for 5 min at 90 °C with 12% Bis-Tris Criterion XT precast gels (Biorad, USA) with 1x XT-MOPS buffer at fixed current of 25 mA for 1.5-2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... 10% glycerol) at 30°C for 20 minutes in the dark and subsequently imaged using the ChemiDoc MP system (Bio-Rad). The gel was then incubated in assay buffer for an additional 10 minutes with the addition of 0.1% SDS and imaged again.
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bioRxiv - Microbiology 2023Quote: ... boiled for 5 minutes at 95°C and loaded onto Mini-Protean TGX Stain Free Gels 4-20% (Bio-Rad, P4568096). The gels were transferred onto 0.45 μm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... Oocyte and embryo samples were boiled at 95 °C for 5 min and separated by SDS-PAGE using Any kD precast Polyacrylamide Gels (Bio-Rad). Blotting was performed onto a nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... in a 100 μL reaction for 2 hours at 37°C and purified using Micro-Bio P-30 spin columns (Bio-Rad). The radiolabeled probe was heated to 90°C for 2 minutes and resuspended in 10 mL of ULTRAhyb Oligo hybridization buffer (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... in 1X TAE buffer at 14 °C on a Bio-Rad clamped homogeneous electric field apparatus (CHEF-DR III, Bio-Rad). 3 V/cm were used with 96 h switch time of 600 s at 120° ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged at 18,000 x g for 30 minutes at 4 °C and the supernatant was incubated with SurebeadsTM Protein A Magnetic Bead (Bio-rad) conjugated with rabbit monoclonal DYKDDDDK antibody at RT for two hours ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were mixed with an equal volume of 2X Laemmli sample buffer and incubated for 5 min at 95°C prior to separation by SDS PAGE on Criterion TGX stain-free 4-15% gradient gels (Bio-Rad). Following SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... 2% formaldehyde gel and separated at 125-150V at 4°C for 1-2 h and then transferred to a Zeta-Probe GT membrane (Bio-Rad) via capillary action overnight (Streit et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... About 15 μg of protein samples from lysates were boiled at 95°C for 5 min in Laemmli sample buffer (Bio-Rad) and resolved in 4-15% gradient SDS-PAGE gels (Mini-Protean TGX ...
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bioRxiv - Physiology 2022Quote: ... Lysates were diluted with 4X Laemmli buffer containing beta-mercaptoethanol and boiled for 10 minutes at 95°C and subjected to SDS-polyacrylamide gel electrophoresis 4-20% (Criterion TGX Stain-Free Protein Gel, Bio-Rad) gels ...
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bioRxiv - Microbiology 2022Quote: ... Samples were heated 10 min at 95°C before loading 10 µl aliquots onto precast mini- PROTEAN TGX gels (Bio-Rad). The polyacrylamide concentration was 7.5% for GFP-DamX constructs ...
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bioRxiv - Microbiology 2022Quote: ... Samples were heated for 10 min at 95°C before loading 10 µl onto a 10% polyacrylamide precast mini-PROTEAN TGX gel (Bio-Rad). After electrophoresis the gel was washed twice with distilled water ...
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bioRxiv - Molecular Biology 2022Quote: ... The plate was then heat sealed with pierceable foil using a PX1 PCR plate sealer 5s at 180°C (Bio-Rad), and PCR amplification was carried out in a T100 thermal cycler (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were heated at 95°C for 10 min and loaded into a 4-15% gradient Tris-glycine gel (Biorad 4561084) immersed in Tris-glycine-SDS running buffer (Biorad 1610772) ...
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bioRxiv - Physiology 2022Quote: ... followed by a final melting curve analysis from 70°C to 95°C with 0.2°C increment for each step using the CFX96 Real-Time PCR System and the Precision Melt Analysis software (Bio-Rad). For HMA analysis ...
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bioRxiv - Plant Biology 2022Quote: ... The dicing reactions were performed at 37 °C for 30 min and stopped by adding 1 volume TBE-Urea sample buffer (Bio-Rad), heating at 95 °C for 10 min and quickly chilling on ice for 2 min ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown at 30°C for 3-6 days and images captured using a Chemidoc XRS+ imager (BioRad, Hercules, CA). All images were evenly adjusted in Photoshop (Adobe Systems Incorporated ...
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β-catenin programs a tissue-specific epigenetic vulnerability in aggressive adrenocortical carcinomabioRxiv - Cancer Biology 2022Quote: Samples were prepared by boiling at 95°C for 6 minutes in lysis buffer supplemented with 4x Laemmli Sample Buffer (Bio-Rad), with 355 mM 2-mercaptoethanol freshly added according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5% of total input and 15% of total IP fraction were resolved on 10% SDS-PAGE gels and wet transferred onto a 0.45 µm nitrocellulose membrane for 2 h at 80 V and 4°C (Bio-Rad, Germany). The membrane was blocked for 1 h in 5% BSA in PBST (PBS + 0.2% Tween20) ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants were mixed with reducing SDS sample buffer and incubated for 5 min at 95°C prior to fractionation by SDS-PAGE analysis on 4–15 % Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Western Blot analysis was performed as described previously (15).
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was then incubated overnight at 4°C with a 1:1000 concentration of anti-6×His tagged monoclonal antibody produced in mouse (ABD Serotec Biorad, UK). Then ...
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bioRxiv - Biochemistry 2024Quote: ... The plates were incubated overnight at 37°C and were imaged the next day using a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Biochemistry 2024Quote: ... Lysates were cleared by centrifugation (10 min at 14,000 x g, 4°C) and quantified by Bradford (Bio-Rad, cat#5000205). All samples were then boiled for 5 min in Laemmli sample buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were boiled at 100°C for 5 min after addition of 50 µl of 4x Laemmli sample buffer (BioRad, 1610747) containing 2-Mercaptoethanol (BME) ...
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bioRxiv - Molecular Biology 2023Quote: ... containing 2-Mercaptoethanol at 95°C for 10 min and separated by electrophoresis in Any kDTM Mini-PROTEAN TGX precast gel (Bio-Rad) and transferred to a PVDF membrane (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... Images were collected following 5 days of incubation at 30 °C with a Bio-Rad Chemidoc MP Imaging System (Bio-Rad) and analyzed using the ImageJ software (50).
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bioRxiv - Cancer Biology 2024Quote: ... The samples were then transferred to a PVDF membrane at 400mA for 2 hours at 4°C in 1x Tris/Glycine buffer (1610771, Bio-Rad). Membranes were then blocked in casein blocking buffer (PI37528 ...
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bioRxiv - Microbiology 2024Quote: ... 30-50 μg of total protein was boiled at 99°C for 5 minutes with the addition of Laemmli Sample Buffer (1610747, Bio-Rad) containing 1% 2-Mercaptoethanol in a total volume of 60-80 μl ...
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bioRxiv - Microbiology 2024Quote: ... The samples were then treated according to the instruction of the beads and the protein complexes were then eluted by incubating at 99°C for 5 minutes with Laemmli Sample Buffer (1610747, Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... Target amplification and real-time signal collection were carried out at 66°C for 60 mins on CFX96 Touch Real-Time PCR Detection System from Bio-Rad.
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bioRxiv - Neuroscience 2024Quote: ... via wet transfer at 30V for 1 hour at 4 °C in 10% Methanol Tris/Glycine buffer using a Criterion™ Blotter (BioRad). Blots were all blocked for 1 hour at room temperature using Intercept (TBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were eluted by heating the beads (95°C for 5 min.) in 70 μL of BME 4X Laemmli buffer (Biorad #1610747). Prior to loading on a 7.5% TGX SDS-PAGE gel (Biorad #5671024) ...
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bioRxiv - Neuroscience 2024Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4 °C and subsequently washed in TBST and incubated for 1 hours at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by a 10 min final extension at 72°C before resolving on a 5% polyacrylamide tris-borate-EDTA (TBE) gel (Bio-Rad) at 35 V ...
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bioRxiv - Plant Biology 2024Quote: ... and 500nmol of each primer and annealing temperature of 59 °C on a CFX Connect Real-Time PCR Detection System (Bio-Rad) at 95 °C for two minutes followed by forty cycles of 95 °C for 15 seconds and 59 °C for 30 seconds ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured at 95°C for 10 min and run on 4-20% Criterion TGX Precast Midi Protein gels (Bio-Rad). Resolved protein was transferred to nitrocellulose using Trans-Blot turbo transfer packs ...
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bioRxiv - Microbiology 2024Quote: ... 20µg of protein samples were heated in 1X Laemmli buffer at 95°C for 5 min and loaded on pre-cast 4-20% SDS-polyacrylamide electrophoresis gel (Bio-Rad). For the RIP experiment ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were centrifuged for 30 min at 20,000 x g at 4 °C and protein concentrations of the supernatants were measured with standard Bradford assay (Bio-Rad Laboratories). 25 or 50 µg of proteins were diluted in Laemmli buffer 3× (188 mM Tris-HCl ...
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bioRxiv - Immunology 2024Quote: ... the proteins were transferred to a polyvinylidene difluoride membrane (110V, 1h, 4°C) using a Mini Trans-Blot cell apparatus (Bio-Rad). Non-specific binding sites were blocked using PBS/Tween 20 (0.05% Tween 20 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were boiled for 10 minutes at 95°C before separation on a gradient of 4-20% SDS-PAGE gels (Bio-Rad). Samples were then transferred to nitrocellulose membrane using standard methods for either semi-dry or wet transfer (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: Samples were incubated at 95°C for 10 minutes and loaded onto SDS-polyacrylamide gels (Mini-Protean TGX gels 4-20%, Bio-Rad). Following gel electrophoresis ...
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bioRxiv - Systems Biology 2023Quote: ... Cell pellets were lysed by heating (95°C for 15 minutes) in a reducing buffer consisting of 90% Invitrogen Laemmli buffer (Bio-Rad) and 10% NuPAGE (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... Samples normalized to equal amounts were denatured in Laemmli’s buffer at 95°C for 10 min and resolved by SDS-PAGE using Criterion TM TGX TM Midi protein gels (Bio-Rad). Resolved proteins were transferred onto 0.2μM PVDF membrane by the Criterion TM Blotter (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... The insoluble material was separated at 13,000 rpm for 30 minutes at 4 °C and total protein concentration was measured on supernatants using Bradford reagent (Bio-Rad). Protein extracts were denatured with NuPAGE®LDS (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... extracted proteins were diluted in SDS loading dye and incubated at 72°C for 10 min and were loaded to 4-20% Mini-PROTEAN TGX gels (Bio-Rad). Immunoblotting was performed with antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Plates were incubated at 30°C and the growth was monitored by image scan using the device ChemiDoc MP (Bio-Rad) every 24 hours ...
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bioRxiv - Biochemistry 2023Quote: ... boiled for 5 min at 98 °C in the presence or absence of β-mercaptoethanol and loaded onto a TGX mini protean precast gel (Biorad) for 50 min at 200V.