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Citations for Bio-Rad :
6351 - 6400 of 7957 citations for Myeloperoxidase MPO Human ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: 250 ng of total RNA of each sample was reversely transcribed and amplified using the TargetAmp-Nano Labeling Kit for Illumina Expression BeadChips (Biozym Scientific GmbH, Hessisch Oldendorf, Germany) on a Biorad MJ Thermal Cycler (Biorad, Munich, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... CLN5 transcripts were measured from the control and CLN5i neurons RNA by quantitative polymerase chain reaction[13] using an iTaq Universal Probes one-step kit (Bio-Rad, 1725141) on a Roche 96-well thermocycler ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleaned samples were solubilized in the previous buffer described above and then quantified with the RCDC Protein Assay kit (Biorad, Hercules, CA). Ten μg of protein from each sample were digested in-solution using both Lys-C and Trypsin ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Thermo Fischer Scientific) and a T100™ thermal cycler (Bio-Rad). The expression of selected genes was determined by RT-PCR using the iQ Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad, France). The results were analyzed using the Bio-Rad CFX Manager software ...
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bioRxiv - Molecular Biology 2020Quote: ... Isolated RNA (50 ng) was subjected to qRT-PCR analysis using iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad) in the 7500 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2019Quote: ... First strand synthesis and real time PCR were performed using iScript kit and iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA) on Light Cycler 480 (Roche ...
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bioRxiv - Genomics 2019Quote: Mammalian cells were embedded in gel plugs and High Molecular Weight DNA was purified as described in a commercial large DNA purification kit (BioRad #170-3592). Plugs were incubated with lysis buffer and proteinase K for four hours at 50°C ...
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bioRxiv - Immunology 2019Quote: ... One microgram of RNA was used to synthesize first strand cDNA using the iScript cDNA synthesis kit from Bio-Rad (Hercules, CA). The primers used for qPCR were synthesized by IDT (Coralville ...
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bioRxiv - Cancer Biology 2019Quote: One microgram of total RNA was used for cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Inc., Hercules, CA). The PCR reactions were performed with 0.2 µl of cDNA template in 25 µl of reaction mixture containing 12.5µl of iQ SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2019Quote: ... Quantification of gene expression was performed by using the KAPA SYBR-FAST One-Step qRT-PCR kit and the CFX-Connect Real-time PCR detection system (Bio-Rad Laboratories) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and total RNAs were obtained according to the manufacturer’s protocol and then quantified and synthesized into cDNA using a iScript cDNA Synthesis Kit (Bio-Rad, Hercules, USA) for SALL4 expression or using a High-capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... we synthetized 100ng of cDNA from immunoprecipitated and isolated polyribosome-associated RNA (see above for detailed procedure) as well as non-precipitated RNA (input) using the iScript cDNA synthesis kit (Bio-Rad, USA). cDNA was then preamplified using TaqMan assay following kit instructions46 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Samples were reverse transcribed from 500 ng of total RNA in a 20 µL volume using iScript cDNA synthesis kit (Bio-Rad Laboratories). Real-time qPCR was carried out on a C1000™ Thermal Cycler (Bio-Rad Laboratories) ...
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bioRxiv - Genomics 2019Quote: ... 1 μg of each RNA sample was converted into single-strand cDNA with random hexamers using an iScript™ Select cDNA Synthesis Kit (Bio-Rad). To validate fusion transcripts ...
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bioRxiv - Bioengineering 2021Quote: ... the homogenate was centrifuged at 16,000g for 5 min and 5 µl of the supernatant was used for protein measurement in a microplate format utilizing the Lowry DC protein assay kit and γ-globulin for generating standard curve (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... PCR was performed using the AceQ qPCR SYBR Green Master Mix kit (Vazyme, Nanjing, China) and the MyiQ2 Real-time PCR system (Bio-Rad, USA) with the following cycling conditions ...
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bioRxiv - Neuroscience 2021Quote: ... the supernatants were transferred into a new set of tubes and protein concentration was determined using Bio-Rad DC™ protein assay kit (Bio-Rad). Western blot protocol is the same as described before ...
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bioRxiv - Cell Biology 2021Quote: ... 25 ng of cDNA template was mixed with the primers to final concentrations of 200 nM and mixed with 5 μl of iTaqTM Universal SYBR® Green Supermix kit (Bio-Rad). The reactions were done in triplicates with the following conditions ...
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bioRxiv - Biochemistry 2021Quote: ... Quantitative cDNA detection was performed via quantitative real-time PCR (RT-qPCR) with the same gene-specific primers using the iQ™ SYBR® Green Supermix Kit (BIORAD). Serially diluted genomic DNA was used as a quantification standard.
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bioRxiv - Biochemistry 2021Quote: ... For analysis of isolated RNA semi-quantitative reverse transcription–polymerase chain reaction (RT) PCR was performed with the iScript™ cDNA Synthesis Kit (BIORAD, Germany) according to the manufacturer’s instructions using 500ng of isolated RNA ...
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bioRxiv - Cell Biology 2021Quote: ... in accordance with the manufacturer’s protocol then performing qPCR using a Sangon Biotech microRNA qPCR kit with SYBR Green (B532461) in a Bio-Rad CFX96™ real-time PCR detection system (Bio-Rad) using the following thermocycling conditions ...
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bioRxiv - Plant Biology 2020Quote: ... Q-PCR was performed on a Bio-Rad IQ5 real-time PCR detection system by using iTaq Universal SYBR Green One-Step RT-qPCR kit (Bio-Rad Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Distinct MagPlex microsphere sets (Luminex Corp) were respectively coupled to viral or control antigens using the amine coupling kit (Bio-Rad Laboratories) according to manufacturers’ instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was transferred to a new tube and protein concentration was measured with a DC Protein Assay Kit (Bio-Rad, 5000111) using a photospectrometer at 750 nm (Molecular Devices ...
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bioRxiv - Microbiology 2021Quote: Bio-Plex assays for cytokines and chemokines were carried out in Bio-Plex 200 system using Bio-Plex Pro Mouse Cytokine Grp I Panel 23-Plex assay kit (Bio-Rad, M60009RDPD) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was subjected to SYBR Green qPCR using iTaq™ Universal SYBR Green Supermix kit from Bio-Rad (Hercules, CA, US) and primers specific to the 5’-end of tRFs and RNA linker ...
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bioRxiv - Plant Biology 2021Quote: ... One microgram was reverse transcribed to generate first-strand complementary DNA (cDNA) using IScript cDNA Synthesis kit (Bio-Rad, Hercules, CA, USA). qRT-PCR experiments were performed with 2.5 μl of a 1:20 dilution of first-strand cDNA and LightCycler 480 SYBR Green I Master mix ...
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bioRxiv - Plant Biology 2021Quote: ... and treated aliquots amounting to 10 ng RNA were used for cDNA synthesis (iScript™ cDNA Synthesis Kit, BioRad, Hercules, CA, USA). Quantitative real-time PCR was carried out using a StepOnePlus (Applied Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: The cell harvesting and extraction of proteins followed the method described previously.10 A protein assay kit (Bio-Rad, Hercules, CA, USA) was used to determine protein concentrations ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from freshly isolated or cultured cells using the Aurum Total RNA Mini Kit (732-6820, Bio-Rad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The soluble protein content was spectrophotometrically determined using bovine serum albumin as a standard (Bradford Protein Assay Kit; Bio-Rad, Hercules, CA).
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bioRxiv - Molecular Biology 2022Quote: ... Signals were visualized with reagents included in the Efficient Chemiluminescence Kit (Vigorous, Beijing, China) and ChemiDoc XRS (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Cell Biology 2022Quote: ... This step was repeated two times and the protein concentration was determined by the RC DC Protein Assay Kit II (Bio-Rad, 5000122). Two times Laemmli buffer was added ...
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bioRxiv - Cancer Biology 2022Quote: ... equal amounts of protein were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a PVDF membrane by Trans-Blot® TurboTM RTA Mini PVDF Transfer Kit (Biorad, USA). Later ...
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bioRxiv - Microbiology 2020Quote: ... All gene transcripts were quantified by quantitative PCR using qScript™ One-Step qRT-PCR Kit (Quanta Biosciences, #95057-050) on CFX96 real-time PCR system (Bio-Rad). Primer sequences for qPCR were as follow ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were quantified using the Kapa SYBR FAST Universal qPCR kit for Illumina sequencing on the CFX384 Real-Time PCR Detection System (Bio-Rad Laboratories). The libraries were diluted to 4 nM stocks and pooled equitably for sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription (RT) was performed using 250 ng of DNase-treated RNA using the iScript cDNA synthesis kit (Bio-Rad Cat#1708890) and the resulting RT reactions were diluted 1:5 with nuclease-free water ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed and homogenized as described elsewhere.30 Protein concentration was determined using a DC protein assay kit (Bio-Rad, 5000116). 10-15 μg of protein was boiled at 95°C for 5 min in Laemmli buffer (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified and normalized PCR products were further pooled into a single library and quantified using qPCR (KAPA Library Quant Kit qPCR Mix, Bio-Rad iCycler). The final library was diluted to 4 nM and sequenced in an Illumina HiSeq 2500 Platform using a 2×250bp RapidRun kit for an average coverage of 160,000 paired reads per PCR product.
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bioRxiv - Immunology 2020Quote: ... Immunoblots were conducted using Mini-Protean TGX stain-free gels and Transfer-Blot Turbo RTA Transfer Kit reagents(Bio-Rad, Mississauga, Ontario). ABCF1 protein was probed with primary anti-ABCF1 antibody (HPA017578 ...
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bioRxiv - Microbiology 2022Quote: ... The amplification kinetics of each product were checked at the end of each cycle by measuring the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad, France). The results were analyzed using Bio-Rad CFX Maestro software ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the cDNA was with Taq Pro Universal SYBR qPCR Master Mix kit (Q712, Vazyme, Nanjing, China) by the CFX96TM Real-Time System (BIO-RAD, USA). The thermal cycles were 95 ℃ for 5 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The fixed and stained gene expression slide was treated with a permeabilization enzyme specific for mouse tongue determined by the Spatial tissue optimization slide kit (10x Genomics, #1000191) for 24 minutes on the thermal cycler (Bio-Rad, 1851197). Synthesis of cDNA and second strand were performed using reverse transcriptase and second strand reaction (primer and enzyme) ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was extracted from cardiac cultures after 4 days on either soft or stiff PDMS (n=4) using the AurumTM Total RNA Mini Kit (Bio-Rad Laboratories). Libraries were constructed by the Purdue Genomics Core Facilities according to standard protocols using TruSeq Stranded mRNA Library Prep Kit (Illumina) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... DNase treatment was performed prior to cDNA synthesis with the iScript™ gDNA Clear cDNA Synthesis Kit (#1725035, Biorad, Mississauga, ON Canada). Quantitative real-time PCR (qPCR ...
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bioRxiv - Genomics 2019Quote: ... Protein concentration was measured using the RC DC (reducing agent and detergent compatible) Protein Assay Kit II (5000122, Bio-Rad; Hercules, CA). For qPCR experiments ...
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Liver X Receptor activation regulates genes involved in lipid homeostasis in developing chondrocytesbioRxiv - Physiology 2019Quote: ... Complimentary DNA (cDNA) synthesis was conducted using the iScript™ cDNA Synthesis Kit with 500 ng of starting RNA (Bio-Rad Laboratories), and combined with 50 μM of forward and reverse primers (for primer sequences ...
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bioRxiv - Cancer Biology 2019Quote: ... Isolated RNA (100 ng) was subjected to qRT-PCR analysis using iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad) performed on the 7500 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Bioengineering 2019Quote: ... For equal loading the protein concentration of the supernatant was determined with BioRad DC protein assay kit II (BioRad#5000116, Cressier, Switzerland) and standard curve with Albumin (ThermoFisher #23210 ...