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Citations for Bio-Rad :
6401 - 6450 of 7957 citations for Myeloperoxidase MPO Human ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... and ampR was measured by quantitative Reverse Transcriptase PCR (qRT-PCR) using the iTaq Universal SYBR Green One-Step kit (Biorad, Hercules, CA) and a CFX96 Touch Real-Time PCR detection system (Bio- Rad ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the membrane was washed three times with TBST and detection was performed with the Clarity™ Max ECL kit for pulldown experiments (Bio-Rad) and Clarity™ ECL kit for all other experiments (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... This step was repeated two times and the protein concentration was determined by the DC Protein Assay Kit (Bio-Rad, Munich, Germany) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... and cDNA was synthesized from 500 ng of RNA using the iScript kit according to the manufacturer’s protocol (Bio-Rad, 170-8891).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... DNase treatment was performed prior to cDNA synthesis with the iScript™ gDNA Clear cDNA Synthesis Kit (#1725035, Biorad, Mississauga, ON, Canada). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Biorad ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was then generated using the Superscript™ III Reverse Transcriptase kit and pre-amplified with a mixture of the tested TaqMan® probes (Table S1) using the SsoAdvanced™ PreAmp Supermix (Biorad). Differential levels of cDNA expression were measured using the droplet digital PCR (ddPCR ...
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bioRxiv - Microbiology 2019Quote: ... and FLAG-cMCF-HA recovered as described above were prepped using the Bio-Rad ReadyPrep 2-D Cleanup Kit (Bio-Rad 1632130). The sample was then loaded onto 11 cm pH 3-10 immobilized pH gradient (IPG ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative RT-PCR was performed by using a Universal SYBR® Green kit and the C1000 Touch Thermal Cycler real-time PCR detection system (Bio-Rad). The EF1α (AT5G60390 ...
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bioRxiv - Physiology 2019Quote: ... Protein concentration was determined in triplicate using a commercial colorimetric assay (Bio-Rad Protein Assay kit-II; Bio-Rad, Gladesville, NSW, Australia). Nuclear and cytosolic fraction enrichment was confirmed by blotting the separated fractions against a nuclear (histone H3 ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was prepared from RNA isolated from the cell lysates using a iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA), and qRT-PCR was conducted as described previously (21 ...
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bioRxiv - Cell Biology 2019Quote: ... A total of 100 ng RNA per sample was subjected to Reverse Transcriptase (RT) by using iScriptTM cDNA Synthesis kit (1708890, BioRad, Hercules, CA). For whole liver ...
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bioRxiv - Developmental Biology 2021Quote: ... Five hundred ng of total RNA (from 21EM15 cells or mouse lenses) or 10 ng of total RNA (from explants) were reverse transcribed using the iScript cDNa Synthesis Kit (Bio-Rad, CA) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and 10-14h after egg laying and reverse transcribed using oligo-dT primers with a One-Step RT kit (Bio-Rad 1725140). The coding sequence for each gene encoding a gene of interest was amplified from a cDNA template using Phusion High Fidelity DNA polymerase (Thermo Fisher F-530XL) ...
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bioRxiv - Physiology 2020Quote: ... The total RNA was purified from genomic DNA contamination then converted to cDNA using iScriptTM gDNA Clear cDNA synthesis kit according to manufacturer’s instructions (Bio-Rad, CA, USA). Commercially available SYBR® Green Glucose Metabolism Array consisting of 84 genes were used (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... Densitometry of the blots was detected using the Enhanced Chemiluminescence Kit HRP (SQ101, EpiZyme, China) in the ChemiDocTM MP Imaging System (Bio-Rad, USA). Information on the primary antibodies used has been listed and provided (Table EV4).
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was exposed using the Enhanced Chemiluminescence Kit HRP (SQ101, EpiZyme, China) in the ChemiDocTM MP Imaging System (Bio-Rad, USA).
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bioRxiv - Cancer Biology 2021Quote: ... and 20 μg of proteins per sample were resolved in 10 or 12% gels (TGX™ FastCast™ Acrylamide kit, Bio-Rad) via electrophoresis ...
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bioRxiv - Molecular Biology 2020Quote: ... The product from the final elution and whole cell lysate supernatant were subjected to silver staining using Silver Stain Plus Kit (BIO-RAD, # 1610449) to check pull down efficiency and purity ...
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bioRxiv - Immunology 2021Quote: ... 30 μg of protein was mixed with an equal volume of 2×Laemmli buffer supplemented with 5% 2-mercaptoethanol (Catalog no. 1610737) then loaded in 10% polyacrylamide precast gel (TGX™ FastCast™ Acrylamide Starter Kit, 10%, catalog no. 1610172) from BioRad Laboratories (Mississauga ...
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bioRxiv - Molecular Biology 2021Quote: ... and quantitative PCR (qPCR) was performed to quantify mRNA abundance using a SsoFast™ EvaGreen® Supermix kit (Bio-Rad, 172-5204,) on a Bio-Rad CFX96 cycler ...
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bioRxiv - Immunology 2020Quote: ... equal protein amounts of the lysates (25 μg) were applied to the Bio-Plex Mouse Cytokine 23-plex Assay Kit (Bio-Rad Laboratories) and measured with the BioPlex200 system and BioPlex-Manager 6.2 according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 30 µg of protein was mixed with an equal volume of 2×Laemmli buffer supplemented with 5% 2-mercaptoethanol (Catalog no. 1610737) then loaded in 10% polyacrylamide precast gel (TGX™ FastCast™ Acrylamide Starter Kit, 10%, Catalog no. 1610172) from BioRad Laboratories (Mississauga ...
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bioRxiv - Cell Biology 2021Quote: ... Cytokine secretion assay was performed using ThermoFisher ProcartaPlex Multiplex Custom Made mouse cytokine 5-plex assay kit following manufacturer’s instructions and analyzed with the Bio- Plex 200 Luminex-based multiplex analysis system (Bio-Rad, Hercules, CA). All samples were analyzed using technical duplicates ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Reverse transcription reactions were performed to synthesize cDNA from 200 ng of total RNA using the iScript™ Select cDNA Synthesis Kit (Biorad, CA). Real-time quantitative PCR (qRT-PCR ...
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bioRxiv - Neuroscience 2021Quote: ... An aliquot was taken to measure radioactivity and protein content using a liquid scintillation counter and a DC protein assay kit (Bio-rad, USA), respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... Gels were stained using the Silver Staining Plus Kit and documented on the ChemiDoc XRS+ system (Bio-Rad Laboratories Inc., Hercules, USA) according to the manufacturers protocol.
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bioRxiv - Genomics 2020Quote: ... the membrane was incubated with peroxidase-conjugated goat anti-mouse IgG and proteins were detected using a chemiluminescence detection kit (Super-Signal West Pico, Pierce) and a ChemiDoc™ MP system (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... and transferred to a 0.20μm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad, Marnes-la-Coquette, France). The membranes were blocked for 30□min in 5% low-fat milk in TBS-0.1% Tween 20 at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The immunoblotting signals were detected using a CCD camera (Intas Biopharmaceuticals, Ahmedabad, India) after application of enhanced chemiluminescence detection kit (Clarity™ Western ECL Substrate; Bio-Rad, Hercules ...
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bioRxiv - Genetics 2022Quote: ... qRT-PCR analysis were performed with iQ-SYBR Green Supermix kit in an iCycler iQ5 system following the instructions of the manufacturer (Bio-Rad, USA). The relative quantifications of mRNA levels were performed using the cycle threshold (Ct ...
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bioRxiv - Immunology 2022Quote: ... Oligo-dT first strand cDNA synthesis was performed from 5 μg total RNA with iScript Advance cDNA Synthesis Kit for RT-qPCR (Bio-Rad; #1725038) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... The blot was then washed in 6x 5 minutes of TBST prior to being developed with Clarity™ Western ECL Substrate kit (Bio-Rad). To examine actin levels as a loading control blot was stripped using Restore™ WesternBlot Stripping Buffer (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription followed by qPCR was performed in the same reaction using the Universal Probes One-Step PCR kit (Bio-Rad Laboratories) and the TaqMan primers/probe mixtures (all from Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... 500 ng of total RNA was used in a 20 µL reaction volume with the Bio-Rad iScript cDNA synthesis kit (Bio-Rad, #1708890) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein measurements were performed using a BCA kit (Pierce) and 150 µg was loaded on a 10-20 % tris/tricine SDS-page gels (Bio-Rad Laboratories) and blotted onto 0,2 µm nitrocellulose membrane (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2022Quote: ... the fluorescence of the EvaGreen dye incorporated into the PCR product was measured at the end of each cycle using SoFast EvaGreen Supermix 2× kit (Bio-Rad, France). A standard curve using gDNA of purified viruses was performed in parallel of each experiment ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein pellet was resuspended in UTC buffer (8 M urea, 2 M thiourea, 4% CHAPS) and quantified using RC DC™ Protein Assay Kit (Bio-Rad). Sample with 80 µg proteins resuspended in rehydration buffer (8 M urea ...
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bioRxiv - Bioengineering 2024Quote: RNA was diluted in nuclease-free water and combined with the reagents from the One Step RT-ddPCR Advanced Kit for Probes (Bio-Rad, USA) and eGFP primer/probe set with final primer concentrations of 900 nM and probe concentrations of 250 nM to measure transgene expression ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of RNA was taken to synthesize complementary DNA (cDNA) conversion reactions were done using an iScript™ Advanced cDNA Synthesis Kit (catalog no. 18080085, Biorad, USA) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription of the RNA was performed to synthesize cDNA following the standard manufacturer’s instructions of the iScript Reverse Transcription Supermix for RT-qPCR kit (Bio-Rad PN 1708841). cDNA was submitted to the University of North Carolina Chapel Hill Advanced Analytics Core (UNCAAC ...
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bioRxiv - Immunology 2024Quote: ... RT-qPCR was conducted using HiScript II One Step qRT-PCR SYBR Green Kit (Q221-01, Vazyme) with gene-specific primer pairs on a CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratories). The thermal cycling protocol was as follow ...
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bioRxiv - Cell Biology 2024Quote: cDNA was synthesized by reverse transcription from 500 ng of total RNA according to manufacturer’s instructions (iScript™ cDNA Synthesis Kit, Bio-Rad Laboratories). Real-time PCR reactions using primers hybridizing with ERG25 or ACT1 transcripts were performed in an CFX96TM Real-Time System C100TM Thermal Cycler (Bio-Rad Laboratories ...
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bioRxiv - Bioengineering 2023Quote: ... After RNA isolation the respective complementary DNA (cDNA) was then synthesized using the Bio-Rad iScript cDNA synthesis kit (Bio-Rad, USA) as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... and luciferase activities were normalized to total protein levels in the WCEs extracts determined using the Bradford assay kit (Bio-Rad Laboratories).
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bioRxiv - Immunology 2023Quote: ... Proteins in the gels were transferred to the PVDF membrane using the Trans-Blot Turbo Kit by the Trans-Blot Turbo transfer system (Bio-Rad, 1704156). Membranes were then incubated in a blocking buffer (Nacalai Tesque ...
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bioRxiv - Microbiology 2024Quote: ... the fluorescence of the EvaGreen dye incorporated into the PCR product was measured at the end of each cycle using SoFast EvaGreen Supermix 2× kit (Bio-Rad, France). A standard curve using the gDNA of purified viruses was performed in parallel with each experiment ...
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bioRxiv - Pathology 2023Quote: ... and the other one was subjected to the western blot assay using the Trans-Blot Turbo RTA Midi 0.45 µm LF PVDF Transfer Kit (Bio-Rad Laboratories, CA). After transferring the total proteins to the PVDF membrane ...
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bioRxiv - Microbiology 2023Quote: ... for real-time PCR analysis of SARS-CoV-2 E genes using the iTaq Universal Probes One-Step RT-PCR Kit (Bio-Rad, USA) and the QuantStudio™ 5 Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged 10 minutes at 13,000 x g and the supernatant was quantified using a DC assay kit (Bio-Rad, Hercules, California). 200 µg of total protein were diluted into a total of 1.5 mL Array Buffer and rocked over the pre-blocked array membrane containing various total RTK antibodies overnight at 4°C ...
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bioRxiv - Genetics 2023Quote: ... Whole cell extracts were sonicated and cleared by centrifugation and protein concentration estimated using the Bio-Rad DC protein assay kit (Bio-Rad Laboratories).