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Citations for Bio-Rad :
6151 - 6200 of 7957 citations for Myeloperoxidase MPO Human ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The concentration of the isolated RNA was determined using Nanodrop and 250ng RNA was converted into cDNA using the iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... Blots were developed using the ECL kit (Seven, Beijing, China) and detected using the ChemiDoc imaging system (Bio-Rad, California, USA).
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bioRxiv - Microbiology 2022Quote: ... The blots were then washed three times by PBST and then visualized using an Omni-ECL Femto Light Chemiluminescence Kit (EpiZyme, SQ201) by a ChemiDoc MP Imaging System (Bio-Rad).
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... Complementary DNA (cDNA) was synthesized from RNA according to the iScript cDNA Synthesis Kit protocol (Bio-Rad Laboratories Inc.; Hercules, USA), and the concentration was determined using a Qubit flex fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... The following qRT-PCR thermocycling protocol was used with the iTaq™ universal SYBR® Green One-Step kit (Bio-Rad): 95 ºC for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... 4995 µL of cell lysis buffer and 20 µL of PMSF 500 mM (Bio-Plex® Cell Lysis Kit, Biorad, #171304011). The lysate was frozen at −80°C for 2 minutes and then ...
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bioRxiv - Immunology 2023Quote: ... we developed a digital droplet RT-PCR (RT-ddPCR) employing the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad) and the QX200 Droplet Digital PCR System (Biorad) ...
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bioRxiv - Cell Biology 2023Quote: RNA was extracted from whole Drosophila animals and converted to cDNA using iScript™ cDNA Synthesis Kit (Bio-Rad, cat#1708891). Primers for qPCR were designed using IDT PrimerQuest (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... and the protein concentrations in each tube were measured by Bradford assay using Quick Start Bradford protein assay kit (Bio-Rad). An equal amount of each sample (30-35 μl) ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μg of RNA template was converted to cDNA using iScript™ cDNA Synthesis Kit (1708891, Bio-Rad, Hercules, CA, USA), according to the manufactures’ instructions ...
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bioRxiv - Microbiology 2023Quote: The bacterial and fungal loads in extracted DNA samples were quantified by RT-qPCR using the iTaq Universal SYBR Green One-Step kit (Bio-Rad). Each reaction was prepared in a final volume of 20 µL containing 5 µL of template DNA ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mixtures contained 100 ng RNA template per sample diluted 2-fold with qPCR solution (iTaq Universal SYBR Green One-Step Kit, Bio-Rad) containing the primers D2S and D2C ...
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bioRxiv - Molecular Biology 2023Quote: ... The reverse transcription was conducted using 500 ng of RNA in a 20 µl volume reaction mixture of iScript cDNA Synthesis Kit (Bio-Rad). 2.5 µl of 1:10 diluted cDNA was added to 7.5 µl of Sensi-FAST SYBR No-ROX (Bioline ...
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The aryl hydrocarbon receptor in β-cells mediates the effects of TCDD on glucose homeostasis in micebioRxiv - Molecular Biology 2023Quote: ... DNase treatment was performed prior to cDNA synthesis with the iScriptTM gDNA Clear cDNA Synthesis Kit (Biorad, #1725035; Mississauga, ON, Canada). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Biorad ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration of the supernatant was measured using the detergent compatible (DC Lowry) protein assay kit according to the manufacturer′s directions (Bio-Rad). For each sample ...
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bioRxiv - Neuroscience 2023Quote: ... All lysates were normalized using bicinchoninic acid (BCA; Pierce BCA Protein Assay Kit) and combined with 4x Laemmli buffer (Bio-Rad). We loaded 50 μg of reduced protein per gel lane and performed transfer with Trans-Blot® TurboTM Transfer System (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... Custom primers were designed to meet several criteria using NCBI Primer Blast followed by running of the complementary DNA (cDNA) synthesis product made using RT-PCR (iScript kit, Cat no. 1708890, Biorad, USA) on an electrophoresis gel ...
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bioRxiv - Neuroscience 2023Quote: ... Immunoreactivity signals were detected with the SuperSignal West Femto Maximum Sensitivity Substrate kit (Pierce) and a Chemico image system (Bio-Rad). Densitometry quantification of protein band intensities was carried out using ImageJ.
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bioRxiv - Cell Biology 2024Quote: ... RNA isolation was succeeded by cDNA preparation from 1µg of total RNA using iScript™ cDNA Synthesis Kit (#1708891; Bio-Rad Laboratories ...
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bioRxiv - Developmental Biology 2024Quote: ... All mRNAs were transcribed using the SP6 mMessage mMachine kit (Fisher Sci #AM1340) and purified using Biorad Microbiospin columns (Biorad #7326250). Two guide RNAs targeting chordin and Three guide RNAs targeting tyrosinase were transcribed using T7 RNA polymerase (NEB #M0251s ...
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bioRxiv - Cancer Biology 2024Quote: scATAC-seq libraries were performed with the Bio-Rad’s SureCell ATAC-Seq Library Prep Kit according to the manufacturer’s instructions (Bio-Rad, Cat# 17004620). Library concentration and fragment size were checked by Agilent 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2023Quote: ... 35 µg of lysate was loaded on 10% SDS-PAGE after quantifying the protein concentration using Bradford assay kit (Bio-Rad). After transferring bands to nitrocellulose membrane Ponceau S staining was performed for checking equal loading of protein on SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... blots were developed using a SuperSignal West Pico plus chemiluminescence substrate kit (Thermo) and imaged using a ChemiDoc MP imaging system (Bio-Rad). Images were analyzed using Image Lab (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... qRT-PCR were performed following the manufacturer’s instructions for the iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad) using 100 ng of total RNA as template ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription (RT) was performed from 2 to 5 µg of RNA with iScript Adv cDNA synthesis kit (Bio-Rad #1725038). The cDNA was diluted 5 times and 1 μL of this was used for each reaction in real-time PCR ...
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bioRxiv - Plant Biology 2023Quote: ... The cDNAs were prepared in a 10μl volume containing 350 ng of plant total RNA that was reverse-transcribed with an iScriptTM cDNA Synthesis Kit using modified MMLV-derived reverse transcriptase (Bio-Rad), a blend of oligo-d(T ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from PMVECs and Rat lung tissue and subjected to cDNA preparation by the reverse transcription reaction using an iScript cDNA Synthesis Kit (#17088890, Bio-Rad). qPCR analysis was performed in triplicate using iQ SYBR Green Supermix (#1708882 ...
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bioRxiv - Plant Biology 2024Quote: ... 10 μL first-strand cDNA was synthesized using 500 ng RNA with iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad, USA) following the manufactureŕs instructions ...
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bioRxiv - Genetics 2024Quote: 500 ng of RNA was converted to cDNA using the iScript cDNA synthesis kit (#1708891, Bio-Rad, Hercules, CA, United States) containing random hexamers according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... For western blotting the proteins were transferred to polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo RTA Mini PVDF Transfer Kit (Bio-Rad) and the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... Biotin signal was detected and quantified by chemiluminescence according to standard LightShift Chemiluminescent RNA EMSA Kit protocol using a ChemiDoc™ Touch Imaging System (BioRad).
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bioRxiv - Genetics 2024Quote: ... and cDNA was generated from 0.5 micrograms of purified RNA from each sample using an iScript cDNA Synthesis Kit (Bio-Rad #1708890). qPCR was conducted using iTaq Universal SYBR Green Supermix (Bio-Rad #1725151) ...
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bioRxiv - Biophysics 2024Quote: ... A luminol-based chemiluminescence assay kit (Chemi-Lumi One L, Nacalai tesque) and chemiluminescence detection system (ChemiDoc Touch MP imaging system, Bio-Rad) were used to detect chemiluminescence signals.
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Dynamic regions allosterically connect the USP14 active site with the proteasome interaction surfacebioRxiv - Biophysics 2024Quote: ... and the units were converted to molar ellipticity based on protein concentration measured in the sample by the DC protein assay kit (Bio-Rad) using WT USP14 as standard ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from Rat lung tissue and subjected to cDNA preparation by the reverse transcription reaction using an iScript cDNA Synthesis Kit (#17088890, Bio-Rad). qPCR analysis was performed in triplicate using iQ SYBR Green Supermix (#1708882 ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from cells using GeneJet RNA Purification kit (Thermo Fischer) and cDNA was synthesized using iScript Reverse Transcription Supermix (Bio-Rad), as previously described (27) ...
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bioRxiv - Microbiology 2024Quote: ... the blots were washed four times again by PBST and visualized using an Omni-ECL Femto Light Chemiluminescence Kit (EpiZyme, SQ201) through a ChemiDoc MP Imaging System (Bio-Rad). To examine the S glycoprotein packaging efficiency ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then blotted onto polyvinylidene difluoride (PVDF) membranes using Trans-Blot Turbo™ Transfer Starter System and Midi PVDF kit (Both are from Biorad). After transfer ...
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bioRxiv - Plant Biology 2024Quote: ... graminicola genomic DNA was quantified by quantitative PCR using Biozym Blue S’Green qPCR Kit (Biozym Scientific, Germany) in a CFX RT-PCR detection system (Bio-Rad, USA) and specific primers for the histone 3 (CgH3 ...
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bioRxiv - Plant Biology 2024Quote: ... higginsianum genomic DNA quantitation was performed by quantitative PCR using Biozym Blue S’Green qPCR Kit (Biozym Scientific, Germany) in a CFX RT-PCR detection system (Bio-Rad, USA) and specific primers for ChTrpC gene (Supplementary Table S1) ...
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bioRxiv - Plant Biology 2024Quote: ... The rRNA depleted was used immediately to prepare the metatranscriptome libraries using the SEQuoia complete RNAseq kit (Bio-Rad cat# 17005726) following the manufacturer’s procedures without any modifications ...
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bioRxiv - Neuroscience 2024Quote: ... the gel was transferred to a LF PVDF membrane using the Trans-Blot Turbo RTA Midi 0.45 µm LF PVDF Transfer Kit (BIO-RAD, 1704275). After blocking in 5% skim milk powder (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were heat at 95°C for 10 min and then separated by SDS-PAGE electrophoresis on 10% acrylamide gels (FastCast™ Acrylamide kit, BioRad) at 110V for 60 min ...
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bioRxiv - Genetics 2024Quote: ... RNA concentrations were measured with the Little Lunatic (Unchained Labs) and cDNA was synthesized using the iScript cDNA synthesis kit (Bio-rad). Primers for skeletal marker genes were designed using NCBI Primer-BLAST (Supplementary Table 3 ...
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bioRxiv - Immunology 2024Quote: ... The following cytokines and chemokines were measured using a Bioplex Express Kit according to the manufacturer’s directions (Biorad, Hercules, CA, USA): GM-CSF ...
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bioRxiv - Microbiology 2024Quote: ... A total of 500 ng of resulting total RNA was used to synthesize cDNA via random priming utilizing the iScript™ cDNA Synthesis Kit (Biorad). Real-time qPCR was performed on QuantStudio 6 Pro (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: RNA extracts were reverse transcribed into cDNA using an iScript™ Select cDNA Synthesis Kit (Bio-Rad, Marnes-la-Coquette, France) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: We carried out reverse transcription with RNA prepared for transcriptome analysis using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). The cDNA product was diluted 10-fold with water and we used 1Lμl per technical replicate for quantitative PCR analysis ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative RT-PCRs (qRT-PCRs) were carried out in triplicate with three technical replicates with the SsoAdvanced Universal SYBR Green kit (Bio-Rad) on a CFX384 Touch Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Immunology 2024Quote: ... from colonic samples of DR3.IL17A-/- (n = 5) and DR3 mice (n = 5) were reverse transcribed to cDNA using the High-Capacity iScript cDNA synthesis kit (Bio-Rad). qPCR reactions were then carried out in triplicate using 50 ng of cDNA and the Applied Biosystems Power SYBR Green PCR Master Mix (Applied Biosystems) ...