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Citations for Bio-Rad :
6251 - 6300 of 6467 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The 1st strand cDNA was synthesised from 1 µg of RNA for each sample using iScript™ cDNA Synthesis Kit (Bio-Rad). This cDNA was then used as the template in qRT-PCR reaction mixture after 10 times dilution ...
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bioRxiv - Pathology 2023Quote: ... Heparin Sepharose 6 Fast Flow beads (1 ml) (Healthcare Life Sciences) were loaded into disposable Bio-Spin® chromatography columns (Bio-Rad) and packed with 2 ml of equilibration buffer (0.15 M NaCl in 25 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... cDNA was diluted 1:10 in nuclease free water prior to qPCR analysis using a CFX384 real-time PCR system (Bio-Rad, USA). Reaction mixtures containing 5 μl PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... protein was diluted to an equivalent concentration for each sample in additional 1% SDS solution with 4X Laemmli sample buffer (Bio-Rad, #1610747) and 2-mercaptoethanol ...
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bioRxiv - Neuroscience 2023Quote: ... Complementary DNA (cDNA) synthesis was performed using 1 μg of total RNA with the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). The genes encoding for the zonula occludens-1 (Zo-1) ...
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bioRxiv - Biochemistry 2023Quote: ... Secondary antibody incubation was done at room temperature for 1h (HRP-conjugated goat anti-rabbit IgG, 1:2000 dilution, Bio-Rad, 403005). Membranes were washed with Tris-buffered saline containing 0.05% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... before injecting into 1 mL Hitrap Heparin HP column pre-equilibrated with Wash Buffer C operated in the BioLogic DuoFlow™ FPLC system (Bio-rad). The protein was eluted from the column by running a gradient flow rate that exchanges Wash Buffer C and Elution Buffer D (2000 mM NaCl ...
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bioRxiv - Bioengineering 2022Quote: ... Levels of inflammatory cytokines were quantified using Bio-Plex Pro Human Inflammation Panel 1 37-plex (#171AL001M) magnetic bead-based assay (Bio-Rad Laboratories) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were detected using chemiluminescence using HRP conjugated secondary antibodies of either anti-mouse (Bio-Rad # 170-6516, dilution 1:10,000) or anti-rabbit (GE Healthcare # NA9340 ...
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bioRxiv - Cancer Biology 2023Quote: ... Complementary DNA (cDNA) was synthesized with 1 µg of total RNA by using the iScript cDNA Synthesis Kit (Bio-Rad, Cat# 1708891). cDNA was diluted 20 times and real-time quantitative PCR was performed by using iTaq Universal SYBR Green Supermix (BioRad ...
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bioRxiv - Biochemistry 2023Quote: ... Fraction collection was performed using a Masterflex L/S easy-load II (Cole Parmer, 77200-50) running at approx 1 mL/min and BioFrac™ fraction collector (Bio-Rad) with a fraction time window of 2 min ...
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bioRxiv - Microbiology 2023Quote: ... before destaining with Tris buffered saline Tween-20 (TBST) and incubation for 1 hour with blocking buffer (TBST with 50 g/L blocking agent (Bio-Rad, 1706404)) ...
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bioRxiv - Microbiology 2023Quote: ... before being incubated for 1 h in water with 3X GelRed (Phenix Research Products) and imaged on ChemiDoc XRS imaging system (Bio-Rad Laboratories) and Quantity One imaging software (Bio-Rad Laboratories).
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bioRxiv - Immunology 2023Quote: ... qPCR reactions were conducted using 1 μl of cDNA product in 10 μl total reaction volume using Itaq Universal SYBR green (Bio-Rad, #1725121) with primers at a concentration of 500nM ...
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bioRxiv - Cell Biology 2023Quote: ... wre incubated for 1 h at RT and membranes were revealed by using ECL reagents with the ChemiDoc imager (Bio-Rad Laboratories).
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bioRxiv - Plant Biology 2023Quote: ... a total of 20 µL of 1 µg/µL plasmid DNA was combined with 50 µL of gold microparticles (1 µm, 50 mg/ml, suspended in 50% (v/v) sterile glycerol) (#1652263, Bio-Rad, USA), 100 µL 2.5 M CaCl2 and 40 µL 0.1 M spermidine (S0266 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The blocking buffer was removed and 10 ml 5% BSA-PBST with 1 μl RABBIT anti-DYKDDDDK Tag antibody (Bio-Rad, # AHP1074), 1 μl anti-MBP Monoclonal Antibody (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked in 5% BSA for 60min at RT and incubated with the primary antibody (anti-NP, Bio-Rad, 1/5000) for 45-60min shaking ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to a nitrocellulose membrane using the iBlot 2 device and membranes were blocked for 1 hour at RT in 5% (w/v) Blotting Grade Blocker/PBS (Biorad, #170-6404). GCase was detected using rb mAb to hGBA antibody (abcam ...
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bioRxiv - Immunology 2023Quote: ... The reactions were stopped with 1 M H2SO4 after 20 min and the absorbance was measured in a microplate reader (Benchmark; Bio-Rad Laboratories) at 490 nm.
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bioRxiv - Neuroscience 2023Quote: ... Samples were prepared for gel electrophoresis by adding a volume containing 20 ug protein to 3 uL 1 M DTT and 7.5 μL 4X Laemmli sample buffer (BioRad Cat. No. 1610747) and topping up to 30 μL with deionized water ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked in 6% milk in PBS and subsequently stained with mouse anti-V5 (clone SV5-Pk1, BioRad, 1:10 000) and goat anti-mouse (1:100 000 ...
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bioRxiv - Immunology 2024Quote: RNA was isolated (ZymoResearch R2050) from the infected Hsu cells at various passages and 1 μg of RNA was used to prepare cDNA (BioRad 170-8891). The cDNA was used as the template for various PCR reactions targeting the 4 genes of interest - E ...
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bioRxiv - Plant Biology 2024Quote: ... and 1 µl of cDNA was used as a template for qRT-PCR using iQTM SYBR® Green Supermix (BIO-RAD, USA) on a CFX96TM Real-Time PCR machine (BIO-RAD ...
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bioRxiv - Developmental Biology 2024Quote: ... and 0.5 μL of forward and reverse primers (see Supplementary Table 1 for primer sequences) for the genes of interest were combined in a 384-well plate (Bio-Rad, HSP3805). The plate was run on a CFX384 Real Time PCR Detection System using the following program ...
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bioRxiv - Developmental Biology 2024Quote: ... see Supplementary Table 1 for primer sequences) and diluted to 20 μL with nuclease free water in a 384-well plate (Bio-Rad, HSP3805). The plate was run on a Bio-Rad CFX384 Real Time PCR Detection System with the following program ...
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bioRxiv - Immunology 2024Quote: ... Pro-inflammatory and pro-fibrotic mediators were measured in 50µl of cell-free tissue culture supernatants using a 37-Plex Bio-Plex Pro™ Human Inflammation Panel 1 (Bio-Rad #171AL001M) according to manufacturer’s instructions ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... concentrated fractions containing CDT holotoxin were loaded onto a gravity flow size exclusion column (1 g, P-60 resin; #1504164, Bio-Rad, USA) pre-equilibrated with PBS pH 7.4 as the mobile phase and 500 μL elution fractions taken.
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then rinsed 3 times with PBS to ensure the complete removal of glutaraldehyde and blocked for 1 h in blocking buffer (5% milk (Bio-Rad #1706404) in Tris-Buffered Saline (TBS ...
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bioRxiv - Immunology 2024Quote: ... were covalently coupled to carboxyl-functionalized fluorescent magnetic beads (1-3µg/1.25*10^6 beads) (Luminex Corp. MagPlex® Microspheres; Bio-Rad; Temse, Belgium) employing the BioPlex amine coupling kit (Ref. ...
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bioRxiv - Neuroscience 2024Quote: ... slices were bombarded with biolistic particles created by coating 1.6 μm gold particles (Bio-Rad, #165-2262; ∼1 μg DNA/mg gold) with constructs in mammalian expression plasmids ...
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bioRxiv - Neuroscience 2024Quote: ... was carried out with 5 ng of cDNA in a volume of 4 μl with 1 μl of 5 μM forward and reverse primers mix and 5 μl of SsoFast EvaGreen Supermix premix (Biorad, CN172-5204). Triplicate reactions were carried out for each mRNA ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were then blocked for 1 hour in 5% non-fat milk in PBS containing 0.1% Tween 20 (Bio-Rad, Hercules, CA) at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... the membranes were washed with 1× PBS and visualised using Pierce™ ECL Plus Western Blotting Substrate with a ChemiDocTM MP Imaging System (Bio-Rad).
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesised from 1 μg of total RNA from each sample following the manufacturer’s instructions of the iScript™ cDNA synthesis kit (Biorad, California, USA). For RNA-Seq validation by RT-qPCR ...
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bioRxiv - Neuroscience 2024Quote: ... Blots were then reprobed using the primary anti-actin 20-33 antibody (MilliporeSigma #A5060; 1:10,000 dilution) and an HRP-linked goat anti-rabbit secondary antibody (Bio-Rad #172-1019).
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bioRxiv - Plant Biology 2024Quote: ... The qPCR reaction was carried out on 2μL of 1/5-diluted cDNA using 2X SSoAdvance SYBR Super Mix (Bio-Rad, Hercules CA) with locus-specific primers (Table S2).
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bioRxiv - Immunology 2024Quote: ... the SVF cell pellets were resuspended in 1 mL and cells were counted using an automated cell counter (Bio-Rad TC-10).
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bioRxiv - Molecular Biology 2024Quote: ... 200 μL aliquots were mixed with 1 μg of the plasmid and transferred to 0.2 cm electro cuvettes (BIO-RAD, Hercules, USA), chilled for 15 min on ice ...
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bioRxiv - Cell Biology 2024Quote: ... The stain-free gel was UV-activated for 1 minute and imaged for total protein (BioRad Chemi Doc XR+, Hercules, CA, USA) with ImageLab software (BioRad Image Lab v6) ...
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bioRxiv - Synthetic Biology 2024Quote: ... the membrane was washed using TBST and incubated for 1 h in a 1:20,000 dilution in 5 % milk of Immun-Star Goat Anti-Mouse (GAM)-HRP conjugate from Bio-Rad (Ontario Canada). Three washes were done ...
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bioRxiv - Systems Biology 2024Quote: Protein concentration of the cell extracts was determined using the Bradford-Lowry method (Quick Start™ Bradford Protein Assay Kit 1, Bio-Rad). For western blotting ...
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bioRxiv - Genomics 2024Quote: ... 1 μg of total RNA was converted into cDNA using an iScript™ gDNA Clear cDNA Synthesis Kit (BioRad, Hercules, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... BPH-1 cell viability was assessed using Trypan Blue staining quantified on a Biorad TC 20 (Bio-Rad, Marnes-la-Coquette, France) after 4 days of treatment with appropriate drugs.
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bioRxiv - Cell Biology 2024Quote: A total of 10 µg of protein was combined with 5 µL of Laemmli buffer (1:9 β-mercaptoethanol [PCS 1610710, Bio-Rad] to Laemmli sample buffer [PCS 161-0737 ...
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bioRxiv - Cell Biology 2024Quote: ... 60°C 1 min]) was then performed using a CFX 384 Touch Real-Time PCR detection system (Bio-Rad, Hercules, CA, USA). Relative quantification was performed using the calibration curve method ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were then washed with TBS-T followed by exposure to the appropriate horseradish peroxidase-conjugated secondary antibody for 1 hr at room temperature and visualized on X-ray film using the enhanced chemiluminescence (ECL) detection system (Bio-Rad Laboratories) or with ChemiDoc Imager followed by acquisition with Image Lab Software (Version 5.2.1).
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bioRxiv - Biochemistry 2024Quote: ... lysates were prepared by diluting samples to contain 20-30 μg of total protein in a 2:1 ratio with 4X LaemmLi sample buffer (Bio-Rad, #1610747) enriched with 100 mM Dithiothreitol (DTT ...
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bioRxiv - Biochemistry 2024Quote: ... The reactions were initiated by adding ATP to a final concentration of 1 mM and incubated in a thermocycler (Bio-Rad T100) at 30°C for 10 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting cDNA was diluted to 20ng/ul and mixed at 1:10 ratio with the SsoAdvanced Universal SYBR Green Supermix (BIO-RAD, 1725271). The qRT-PCR reactions were performed using the CFX96 Real-Time PCR System [C-1000 Touch Thermal Cycler] (BIO-RAD ...