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Citations for Bio-Rad :
6101 - 6150 of 6467 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of total RNA was reverse transcribed to cDNA using the iScript™ gDNA Clear cDNA synthesis kit (Bio-RAD). qPCR was performed with Applied Biosystems Fast SYBR Green master mix on an Applied Biosystems QuantStudio 6 Flex system ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were detected by using horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-mouse IgG (H+L)-HRP conjugate (1:10,000, Bio-Rad #1706516), goat anti-rabbit IgG (H+L)-HPR conjugate (1:10,000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription was carried out for 1 hour at 42°C using the iSCRIPT cDNA synthesis kit (Bio-Rad, Cat #1708841BUN) and 300 ng - 1 μg of RNA per reaction ...
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bioRxiv - Cell Biology 2022Quote: ... 5 µl HeLa lysate was preincubated with 1 µl of accessory protein for 10 min at room temperature in PCR plates (HSP9601, Bio-Rad). Reaction was started by adding 2 µl reaction mix ...
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bioRxiv - Genetics 2022Quote: ... Membranes were washed three times with PBS-T for 5 min followed by 1 minute incubation with ECL detection reagent (Cytiva, RPN2106) and imaged with ChemiDoc MP Imaging System (Bio-Rad). Membranes were washed twice in TBS and stripped before reprobing according to stripping reagent manufacturer’s protocol (Thermo Scientific ...
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bioRxiv - Genomics 2022Quote: ... and incubated at 65°C for 3 mins before running 1% agarose-Etbr gel electrophoresis to detect the cleavage efficiency by ChemiDoc system (Bio-Rad).
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bioRxiv - Immunology 2022Quote: ... then blocked for 1 to 3 hr at RT with 50 μL/well of blocking buffer (HN: 5% Blotting Grade Blocker, Bio-Rad cat ...
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bioRxiv - Immunology 2022Quote: ... and primers to the editing locus producing amplicons of <500bp (1× assay, 900-nM primers and 250-nM probe) using ddPCR supermix for probes (no deoxyuridine triphosphate [dUTP]) (Bio-Rad). Reference reactions were simultaneously performed using a 5’ HEX labeled probe/ primer combination targeting the houskeeping gene B2M ...
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bioRxiv - Plant Biology 2022Quote: ... SybrGreen real-time quantitative PCR experiments were performed with a 1:20 dilution of cDNA using a CFC384 Real-Time System (Bio-Rad). Data were analyzed with the comparative 2ΔΔCt method using the geometric mean of Ef1α (5’-CTG GAG GTT TTG AGG CTG GTA T-3’ and 5’-CCA AGG GTG AAA GCA AGA AGA-3’ ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated with 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit or anti-mouse secondary antibodies (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... Plates were then washed six times with PBS-T and incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG (H + L) (1:2000) (Bio-Rad) diluted in 2% horse serum/PBS for 1 hr at room temperature with agitation ...
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bioRxiv - Microbiology 2022Quote: ... and the RNA profile was measured by absorbance (254 nm) across the gradient in real time using an EM-1 Econo UV Monitor (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... and probed in triplicate by qPCR for the presence of the 1 kb capsule fragment using iTaq DNA polymerase (BIO-RAD) and primer pair DDL34-DDL35 ...
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bioRxiv - Microbiology 2022Quote: ... the generated cDNA was diluted (1:20) and amplified using stat1a- or stat1b-specific primers and 2x SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) in Hardshell® Low Profile Thin-wall 96-well skirted PCR plates (Bio-Rad ...
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bioRxiv - Physiology 2022Quote: ... diluted 1:2500 in PBS-Tween 5% milk was used to detect primary antibodies bound to the blot by BIO-RAD ChemiDoc imaging system ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA (1 μg) was reverse transcribed (RT) using the iScript Reverse Transcription Supermix for RT-PCR kit (#170±8840 Bio-Rad), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 μL re-suspended Fraction B pellets were run 1 cm onto an Any kD Mini-Protean TGX gel (Bio-Rad) followed by Coomassie Blue staining ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were then spun for 1 min at 20k x g and run on a 10% SDS gel before transferring to nitrocellulose membranes (Bio-Rad) and blocking as described above ...
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bioRxiv - Bioengineering 2022Quote: ... After transferring the proteins to nitrocellulose membrane using a transfer buffer (25 mM Tris/192 mM glycine/0.1 (w/v) SDS/20% MeOH) for 1 h at 150 mA in a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad), the membrane was saturated for 2 h at RT with 5% semi-skimmed milk in PBS-Tween (0.1% ...
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bioRxiv - Bioengineering 2023Quote: ... Slides were then washed with 1x TBS before blocking in a 1% bovine serum albumin (5000206; Bio-Rad, Hercules, CA, USA), and either 5% donkey (D9663-10ML ...
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bioRxiv - Bioengineering 2023Quote: ... 1 µL of the formed cDNA was used in 10 µL reactions using the SsoAdvanced universal SYBR green supermix (Bio-rad) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were transferred onto PVDF membranes (activated in methanol for 1 minute) using the Trans-Blot® TurboTM Transfer System (BioRad). Transferred membranes were washed in tris-buffered saline (TBS ...
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Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeastbioRxiv - Genetics 2023Quote: ... the membrane was blotted with the Chk1-pS345 antibody at the 1:3000 dilution for 3 h to detect the phosphorylated Chk1-Ser345 in ChemiDoc (Bio-Rad). The membrane was stripped ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was then diluted 1:100 and 1.5 µL was use per 15 µL reaction with iTaq Universal SYBR Green Supermix (Bio-Rad # 1725122) and 250 nM primers (final ...
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bioRxiv - Biochemistry 2023Quote: ... 20 minutes) and the clear lysate was purified on HisTrap HP 1 ml affinity column (Cytiva) by FPLC system (NGC™, BioRad). The protein was eluted from the column using a linear gradient (Elution buffer ...
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bioRxiv - Biochemistry 2022Quote: An equivalent volume of ∼150 µg FBS was first diluted 1:2 with loading buffer (consisting of 2X Laemmli Sample Buffer (#1610737, Bio-Rad) plus a 5% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... flash frozen in liquid nitrogen and treated with a lysis buffer (150mM NaCl, 50mM Tris pH7.5, 1% Triton X-100 (Bio-Rad #1610407), 1% SDS ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were washed three times (10 min each) and probed by incubation for 1 h with the appropriate secondary antibodies conjugated with horseradish peroxidase (Bio-Rad). The membranes were then washed three times (15 min each at room temperature) ...
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bioRxiv - Genetics 2023Quote: ... China) using TBE (65 mM Tris-HCl, 27 mM boric acid, 1 mM EDTA, pH 9; Bio-Rad, Hercules, CA, USA) as the buffer ...
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bioRxiv - Neuroscience 2023Quote: ... 10-30 μg of protein extracts were run at 100V for 1 hr on 10% pre-cast SDS-PAGE gels (Bio-Rad), then electro-transferred to 0.2 μm nitrocellulose membranes using the Trans-Blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µg of total RNA from each sample was converted to cDNA using iScript™ Reverse Transcription Supermix (Bio-Rad, 1708841). Then qPCR reactions were set up using TaqMan® Fast Advanced Master Mix (ThermoFisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... Membranes were then washed three times with and incubated with StarBright Blue 700 goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... After gel polymerization was performed pre-electrophoresis of empty gel in 5% acetic acid (running buffer) for 1-1.5h/150 V in reverse polarity of electrophoretic BioRad system (Bio-rad, USA). The running buffer was discarded ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were separated on 10 % polyacrylamide gels and blotted onto a nitrocellulose membrane at 100 V for 1 h using the Mini Trans-Blot system (Bio-Rad). Membranes were blocked with 5 % milk powder in PTw (1x PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies were applied for 1 hour at room temperature (1:5,000; CST, #7074) before detecting protein bands at the BioRad ChemiDoc MP Imaging System using chemiluminescence (BioRad, 170-5061).
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bioRxiv - Molecular Biology 2024Quote: ... The HRP-linked secondary antibodies (CST: 7076S) were used by 1:4000 dilution and the blots were visualized with the ECL reagents (Bio-Rad).
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bioRxiv - Physiology 2024Quote: ... Blotted bands were detected using HRP conjugated secondary antibodies goat anti-rabbit conjugated with HRP (#1706515, Bio-Rad, 1:15, 000). ImageJ was used to calculate the fluorescence density of each band ...
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bioRxiv - Molecular Biology 2024Quote: ... was added to all samples and the products were separated on 15% polyacrylamide denaturing urea gels (19:1 acrylamide-bisacrylamide, Bio-Rad). The gels were fixed in fixing solution (40% methanol ...
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bioRxiv - Neuroscience 2024Quote: ... Signal was developed with application of SuperSignal West Dura ECL substrate (Fisher, cat# PIA34075) (1 mL per blot) and visualized using a ChemiDoc XRS+ Imaging System (Bio-rad). Consistent exposure times were acquired for each sample set and probe ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR Products were gel isolated from a 1% TAE agarose gel using Freeze ‘N Squeeze DNA gel extraction columns (Bio-Rad), following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of total RNA was DNase treated and reverse transcribed using the iScript gDNA clear cDNA Synthesis kit (BioRad, 1725035). CDSs were then amplified with sequence specific primers annealing at the start (forward ...
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bioRxiv - Molecular Biology 2024Quote: ... ChIP/Input DNA samples were analysed using primer sets for specific genomic regions (Table 1) using iQ SYBR-Green Super mix (Bio-Rad) on a CFX384 Real-Time PCR system (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed five times with PBS-T and incubated with HRP-coupled secondary antibody for 1 h at room temperature and imaged with the ChemiDoc Imaging System from Bio-Rad. Relative protein levels were quantified with Image Lab (BioRad ...
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bioRxiv - Immunology 2024Quote: ... Membranes were washed then incubated for 1 hour at room temperature with secondary antibodies (StarBright blue 520 goat anti-mouse IgG and StarBright blue 700 goat anti-rabbit IgG, each at 1:2500, Bio-Rad) and tubulin (hFAB Rhodamine ...
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bioRxiv - Microbiology 2024Quote: ... The abundance of PtfoR in the input DNA (diluted 1:100) and output DNA was then quantified using quantitative PCR with iTaq Universal SYBR Green Supermix (Bio-Rad). A fold enrichment value was then generated by measuring the abundance of the tfoR promoter in the output (following immunoprecipitation ...
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bioRxiv - Immunology 2024Quote: ... membranes were repeatedly washed with TBST and incubated for 2 h with the appropriate HRP-conjugated secondary mouse antibody (1/1500, BioRad Laboratories) or secondary goat anti□rabbit IgG HRP (1/5,000 Cusabio Cedarlane ...
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bioRxiv - Immunology 2024Quote: ... In the case of purified protein 1 μg protein was loaded per condition and the Precision Plus Dual Color protein marker (Bio-Rad) was used as size standard ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 ng template library DNA[21] in a total reaction volume of 20 µL using T100 Thermal Cycler (BIO-RAD). The PCR amplification cycle was as follows ...
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bioRxiv - Neuroscience 2024Quote: ... The gel was electrophoresed for 10 minutes at 50V then 1 hour at 120V in Tris-Glycine SDS buffer (Bio-rad). The gel and nitrocellulose membrane were then equilibrised in transfer buffer (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... was added to each sample and heated at 95 °C for 1 hour in a thermal cycler (S1000 with 384-well reaction module, Bio-Rad) set to a constant lid temperature of 110 °C ...