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Citations for Bio-Rad :
6151 - 6200 of 6467 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... the GRB2 protein was eluted by linearly increasing the concentration of Imidazole from 20 to 500 mM with a flow rate of 1 ml/min in buffer A using Econo Gradient Pump (BIO-RAD). The fractions containing the target protein were concentrated to 5 ml with Amicon Ultra-15 10 kDa (Merck) ...
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bioRxiv - Neuroscience 2024Quote: ... Viability of magnetically-captured cells (eluted into a new tube away from the magnet) was assessed by trypan blue staining (1:2 dilution sample in trypan blue; BioRad 1450013). Cell viability was quantified as the proportion of cells that did not take up the dye ...
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bioRxiv - Biochemistry 2024Quote: ... RNA concentration was measured and 1 μg was used to synthesize cDNA using iScript™ Reverse Transcription Supermix (Bio-Rad, 1708891) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... After final wash and supernatant removal, 1x Loading Buffer (40 μL, 3:1 Lysis Buffer:4x Laemmli Loading Buffer (Bio-Rad)) was added to the sample and the sample boiled (10 min ...
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bioRxiv - Microbiology 2024Quote: ... The cell mixture was transferred to a 1 mm electroporation cuvette and pulsed using a Gene Pulser Xcell Electroporation System (Bio-Rad) using 2.1 kV/cm ...
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bioRxiv - Physiology 2024Quote: ... The cells were centrifuged at 1500 rpm for 10 mins at 4°C and the pellet was resuspended in 5 ml single-cell suspension buffer (2 mM EDTA, 0.5 BSA, 1% FBS in DPBS) and the cells were counted (Bio-Rad TC20) to prepare 1×106 aliquots.
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bioRxiv - Neuroscience 2024Quote: Microglia was assessed in brain sections using a monoclonal rat antibody against CD68 (1:200, MCA1957GA, Bio-Rad, Hercules, CA, USA) to label macrophage/microglia and a polyclonal rabbit antibody against Iba-1 (1:1000 ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples from the co-IP and were separated on 8% SDS-PAGE gels for 1 h at 120 V and transferred overnight at 20 V on to PVDF membranes (Bio-Rad). Blots were probed with rabbit anti-NMIIB/Myh10 (1:2000 ...
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bioRxiv - Microbiology 2024Quote: ... was added to the membrane and incubated for 1 minute at room temperature before imaging in the Image Lab software on a ChemiDoc MP Imaging System machine (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... diluted in 1% BSA-TBST (1:10,000) was used in combination with a goat anti-rabbit secondary antibody coupled with horseradish peroxidase (BioRad, 170-6515) diluted in 1% BSA-TBST (1:5,000) ...
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bioRxiv - Microbiology 2024Quote: ... and the RNA profile was measured by absorbance (254 nm) across the gradient in real time using an EM-1 Econo UV Monitor (Bio-Rad). All fractions with large polysomes ...
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bioRxiv - Molecular Biology 2024Quote: ... for 1 hr 30 min followed by 5 washes with PBST and staining with Goat-anti-rabbit IgG StarBright Blue 700 (1:2,500; Bio-Rad, 12004161) for 1 hr ...
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bioRxiv - Neuroscience 2024Quote: ... Then membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-rabbit IgG-HRP, 1:10 000, Bio-Rad, # 170-6515 ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were incubated for 1h with HRP-conjugated goat secondary antibodies against mouse or rabbit immunoglobulin (1:3000, 170-6516, 170-6515, Bio-Rad). Proteins were visualized on an iBright™ CL1000 Imaging System (ThermoFisher ...
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bioRxiv - Genomics 2024Quote: RT□qPCR was performed with a 1:10 dilution of cDNA using the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, US) in a final reaction volume of 10 μL ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized starting from 1-2 μg of total RNA using the iScript cDNA Synthesis Kit (Bio-Rad, catalog #1708891). RT-qPCRs were carried out with the Luna® Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... After 1-hour incubation at room temperature the reactions were terminated by addition of 4x Laemmli sample buffer (without β-mercaptoethanol) (BioRad), subjected to SDS-PAGE ...
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bioRxiv - Bioengineering 2024Quote: ... After transferring the proteins to nitrocellulose membrane using a transfer buffer (25 mM Tris/192 mM glycine/0.1 (w/v) SDS/20% MeOH) for 1 h at 150 mA in a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad), the membrane was saturated for 2 h at RT with 5% semi-skimmed milk in PBS-Tween (0.1% ...
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bioRxiv - Cell Biology 2024Quote: ... Electroporation was performed twice (1-minute interval) using 10 μg of each plasmid DNA and a Gene Pulser X cell electroporator (Bio-Rad) with a 2-mm gap cuvette ...
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bioRxiv - Cell Biology 2024Quote: ... First-strand cDNA was synthesized from 1 µg of total RNA using iScriptTM Reverse Transcription Supermix for RT-qPCR (Bio-Rad) and diluted 1:20 in Milli-Q H2O ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were detected using Pierce ECL Western Blotting Substrate for 1 min before being imaged on a molecular imager (ChemiDoc XRS+, Bio-Rad) and analyzed by Image Lab.
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bioRxiv - Plant Biology 2020Quote: ... The cDNA was used as template for real-time PCR (primers in Supplemental table 1) using CFV384 Touch™ Real-Time PCR Detection System (Bio-Rad). Housekeeping genes (AT4G34270 ...
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bioRxiv - Biophysics 2021Quote: The removal of DDM in the protein solutions was done by fixing the protein concentration at 1 mg/ml and using 30 mg of BioBeads SM2 (Bio-Rad, CA) per mg of protein ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 1 μg of RNA was subjected to retrotranscription using iScript™cDNA Synthesis Kit (Bio-Rad, Cat. No. 1708890), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... slices were bombarded with biolistic particles created by coating 1.6 μm gold particles (Bio-Rad, #165-2262; ∼1 μg DNA/mg gold) with constructs in mammalian expression plasmids ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated secondary anti-mouse antibody (Amersham; NA931S) and anti-rabbit antibody (Amersham; NA934VS) at 1:10,000 and Clarity western ECL substrate detection kit (Bio-Rad; 170-5060) were used to detect the signals ...
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bioRxiv - Developmental Biology 2020Quote: ... 1-20μg of tissue protein to be analyzed were combined with equal parts 2X Laemmli sample buffer (Bio-Rad, catalog 161-0737) containing 10% β-mercaptoethanol ...
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bioRxiv - Physiology 2020Quote: ... Gene expression was assessed using commercially available Taqman primer/probe sets (Thermo Fischer) or specific oligonucleotide pairs (Table 1) and SYBR® Green supermix (Bio-Rad). Relative expression of the target genes was calculated with the comparative Ct method [43] using glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Immunology 2021Quote: ... in 0.1% BSA in 0.1% TBST and subsequently imaged using HRP-conjugated anti-rabbit antibodies (Bio-Rad Cat#172-1019, 1:10000) and Western Lightning Plus-ECL (PerkinElmer Cat#El103001EA ...
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bioRxiv - Microbiology 2021Quote: ... Gels were stained with ethidium bromide (0.5 mg mL-1) and photographed using the Quantity One electrophoresis analysis system (Bio-Rad, CA, USA). Gel images were digitally normalized to a single DNA marker to reduce gel-to-gel banding pattern variability ...
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bioRxiv - Neuroscience 2020Quote: ... Up to 1 μg of RNA was used to synthesise cDNA using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad, 1725035) and PowerUp SYBR Green (Thermofisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Gel electrophoresis was performed on a 1% agarose gel and imaged using Molecular Imager® Gel Doc™ XR (Bio-Rad, USA).
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bioRxiv - Cell Biology 2021Quote: ... and 1 μg of RNA was used to generate cDNA with the iSCRIPT Clear kit (Bio-Rad, Hercules, CA, cat# 170-8891). For the qRT-PCR ...
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bioRxiv - Systems Biology 2021Quote: Samples were normalized to 1 mg/mL protein in 0.1% Azo by the Bradford assay (Bio-Rad, Hercules, CA, USA, Cat# 5000006) using bovine serum albumin to generate a standard curve ...
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bioRxiv - Plant Biology 2020Quote: ... or 1 μl (for the rest) of a 1/2 dilution of the cDNA was added to a reaction containing 1X of SsoFast EvaGreen (Bio-Rad, USA), 0.5 μM Forward and Reverse primers (Table 2) ...
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bioRxiv - Plant Biology 2020Quote: ... we used 2 µl of a 1/5 dilution of the cDNA obtained as above in a reaction containing 5 µl of SsoFast EvaGreen (Bio-Rad, USA), 0.5 µl of Forward primer (10 µM ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were washed three times in TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Bio-Rad 1,706,515, 1:10,000) secondary antibodies for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribonucleoprotein (RNP) complexes were made at a 9:1 ratio of sgRNA:Cas9 (90pmol:10pmol) in Gene Pulser® Electroporation Buffer (Bio-Rad, 1652677) and incubated for 10 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using iScript Reverse Transcription Supermix for RT-PCR (Bio-Rad Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... All qPCR amplifications were performed in duplicate and each contained 15 µL total volume of 1 × SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, USA), 0.3 µM of each primer ...
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bioRxiv - Cancer Biology 2021Quote: ... 60 of High Capacity NeutrAvidin slurry (Thermo PI29204) were washed three times with 1 mL 2X RIPA/1mM EDTA buffer in 2-mL chromatography columns (Bio-Rad 7326008) attached to a vacuum manifold (Promega A7231) ...
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bioRxiv - Microbiology 2022Quote: ... The melt curve analysis was performed at 65°C for 1 minute in CFX connects TM Real-time PCR detection system (Bio-Rad, USA). The primers used are listed inTable 1 ...
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bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
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bioRxiv - Microbiology 2022Quote: ... 300 nM concentration of each primer targeting the viral DNA substrate (5’- AGCGTGGGCGGGAAAATCTC-3’) and the indicated target DNA (table 1) and 1X iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). The qPCR cycling conditions for quantifying INS activity included an initial incubation at 95°C for 3 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... 4 ul of diluted cDNA (1:40 dilution rate) were used for each reaction with 5 ul SsoAdvanced Universal SYBR® Green Supermix (BIO-RAD) and 1 ul of primer mix (5uM) ...
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bioRxiv - Bioengineering 2022Quote: ... The PVDF membrane was then washed 3 × 10 min with TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse (diluted 1:20,000; Bio-Rad, Hercules, CA) secondary antibody for one hour at room temperature followed by 6 × 10 min washes with TBST and detection using the Radiance Plus chemiluminescence substrate (Azure Biosystems ...
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bioRxiv - Biophysics 2022Quote: ... TMEM87A in buffer 1 was exchanged into soybean l-α-phosphatidylcholine (Soy PC, MillaporeSigma) with the addition of Biobeads SM2 (Bio-Rad) and an hour incubation at a protein:lipid ratio of 1:10 (corresponding to 0.4 mg purified TMEM87A and 4 mg of Soy PC lipid or 1:50 in buffer (5 mM HEPES pH 7.2 ...
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bioRxiv - Biophysics 2022Quote: ... Electrophoresis was carried out for 1 hour at 90 V using an electrophoresis system (mini–DNA SUB CELL, Bio-Rad, Mississauga, Canada). The DNA fragments were visualized in a Gel Doc XR+ Gel Documentation System (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized using 1 μg of input RNA with an iScript™ cDNA Synthesis Kit and MyIQ thermocycler (both Bio-Rad) and stored at −20 °C overnight ...
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bioRxiv - Plant Biology 2021Quote: The cDNA synthesis was performed using 1 μg of total RNA and iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) following manufacturer’s instructions ...