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Citations for Bio-Rad :
5751 - 5800 of 5853 citations for 6 Chloro 5 6 dihydroimidazo 1 5 a pyrido 3 2 e pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of total RNA was used as the template to synthesize cDNA using the iScript RT-PCR kit (Bio-Rad, Carlsbad, CA). qPCR was performed with the SYBR Green I nucleic acid gel stain (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in cell lysate was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Catalogue #: 12004167; Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
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bioRxiv - Immunology 2023Quote: ... The expression of immune system markers were mostly studied with inmunohistochemistry using the following antibodies: mouse anti rat CD68 (dilution 1:300 BIORAD, ref. MCA 341R) for monocytes/macrophages ...
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bioRxiv - Microbiology 2023Quote: The resulting amplicons and their corresponding lengths were visualized and documented via gel electrophoresis and analysis (Quantity 1 software, Bio-rad, CA, USA).
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bioRxiv - Systems Biology 2023Quote: ... Proteins were transferred to a nitrocellulose membrane (Carl Roth, 200H.1) using a Trans-Blot® Turbo™ Transfer Starter System (BioRad, 1704150). Membranes were stained with Ponceau S (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... and 1 mM DTT) to a final KOAc concentration of 75 mM and incubated with hydroxyapatite Bio gel HTP (Bio-Rad #130-0402) for 45 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Equal concentrations of RNA (measured by NanoDrop and 1 µg for the caecum tissue) were reverse transcribed to cDNA using the iScript™ cDNA Synthesis Kit (Bio-Rad, #1708891) and stored at −20°C ...
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bioRxiv - Molecular Biology 2023Quote: ... at room temperature for 1 h with slight shaking and SP-A-S protein or SP-A-RBD complexes were detected by adding HRP-conjugated Goat Anti-rabbit IgG (1:2000, Bio-rad, Hercules, USA) for an additional 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... The purified RNA was quantified by NanoDrop and 1 µg of RNA for each sample was used to generate cDNA using the iScript cDNA synthesis kit (Biorad cat. no. 1708890). Real-time PCR was performed with PowerUp SYBR Green Master Mix (Applied Biosystems cat ...
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bioRxiv - Immunology 2023Quote: ... where absolute quantification was done by the QX200 Droplet Digital PCR System in combination with the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, United States). The limit of detection was calculated to be 10 copies per reaction.
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... RNA copy numbers were determined by ddPCR (see AAV virus quantification) using Taqman primers for the WPRE element (Sup. Table 1) and Rpp30 (Biorad, assay ID: dMmuCPE5097025) as housekeeping gene.
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bioRxiv - Immunology 2023Quote: Eluted samples were run for about 1 cm on Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (#4569034, Bio-Rad), and the gel was stained with Pierce™ Zinc Reversible Stain Kit (#24582 ...
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bioRxiv - Molecular Biology 2023Quote: ... and optic density of the bands corresponding to mouse PRM1 and PRM2 was quantified using Quantity One 1-D analysis software (BioRad, Hercules, CA, USA) to calculate the PRM1/PRM2 ratio ...
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ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal α-motoneuronsbioRxiv - Neuroscience 2023Quote: ... Gels were run in MOPS buffer at 150 V for 1 hour and 15 min and the proteins transferred for 30 min at 100 V on Millipore PVDF membranes (BIORAD Criterion Blotter apparatus). Membranes were blocked for 1 hour at RT with 4% non-fat dry milk (NFDM ...
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bioRxiv - Genomics 2023Quote: ... 4 μl of 1:10 dilution of cDNA was mixed with SsoAdvanced Universal SYBR Green Supermix (cat no 1725271, Bio-Rad, Hercules, CA) and 500 nM each primer up to a total volume of 10 μl and amplified in a CFX96 Real-Time System (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... total RNA (1 µg) was taken from the cDNA preparation using iScript™ cDNA Synthesis Kit (#1708891, Bio-Rad Laboratories, Hercules, CA, USA). Prior to cDNA synthesis ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C in a humidified chamber with the following primary antibodies: Guinea-pig anti-Insulin antibody (Biorad 5330-0104G, 1:250). Following overnight incubation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We incubated the reaction for 1 hour at 50°C in a thermal cycler (Bio-Rad C1000 Touch Thermal Cycler, Bio-Rad, USA).
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bioRxiv - Systems Biology 2023Quote: Proteins were transferred to a nitrocellulose membrane (Carl Roth, 200H.1) using a Trans-Blot® Turbo™ Transfer Starter System (BioRad, 1704150). Membranes were stained with Ponceau S (Sigma ...
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bioRxiv - Immunology 2024Quote: ... cells were lysed in buffer containing 1% NP-40 and proteins were separated using SDS-PAGE (Mini-PROTEAN® TGX™ Precast Gels, Bio-Rad), transferred to low fluorescence PVDF membranes (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: IgE receptor cross-linking (IgECL) on HSMCs was accomplished through sensitization with 1 μg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA) for 24 hours ...
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bioRxiv - Plant Biology 2024Quote: ... Diluted cDNA (1:50) was used as a template for quantitative PCRs using iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, USA). Real-time detection of fluorescence emission was performed on a CFX384 Real-Time System (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... Goat anti-mouse antibody conjugated to horseradish peroxidase (HRP) was used as a secondary antibody (1:2000) for chemiluminescence detection (Clarity Western ECL Substrates, Bio-Rad, Hercules, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Proteins were detected by chemiluminescence following Western blotting using HRP conjugated secondary antibodies of anti-mouse (Bio-Rad # 170–6516; dilution 1:10,000), anti-rabbit (GE Healthcare # NA9340 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Eggs were subjected to experimental electroporation parameters (voltage = 50 V; pulse = 25 ms; number of pulses = 1) using the Gene Pulser Xcell Eukaryotic System (Bio-Rad, cat. 1652661). Immediately ...
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bioRxiv - Cell Biology 2024Quote: ... 60 μl 1x Laemmli buffer (diluted by mixing same volume of 1 x PBS with 2x Laemmli buffer, Bio-Rad, Catalog number: #1610737EDU) was added to the beads and incubated for 10 min at 70°C ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg RNA was synthesized to cDNA by reverse-transcription using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA; item # 1708891). Real-time PCR was performed in 10 µl reactions containing 5 µl SsoFast EvaGreen Supermix with Low ROX (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... The suspended cells were then diluted 1:100 in PBS and counted using a TC20 automated cell counter (Bio-Rad Laboratories, Hercules CA). For the first vaccination with cda1Δ2Δ3Δ (given to CBA/J ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were separated by SDS-PAGE electrophoresis at 100 volts (V) for 1 h on 4–20% Criterion™ TGX Stain-Free™ Protein Gels (1656001, Bio-Rad) and 1x SDS-page running buffer (192 mM glycine ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used (see also Supplementary Table 1 for details): mouse monoclonal IgG1 anti-βCYA (Bio-Rad, clone 4C2, RRID:AB_2571580), mouse monoclonal IgG2b anti-γCYA (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were immunoprobed using an HRP-conjugated secondary antibody (dilution 1:3000) and chemiluminescent substrate (Clarity Western ECL substrate, Bio-Rad, Hercules, CA, USA). Blot image acquisition was performed using a Molecular Imager® Gel Doc™ XR+ system (Bio-Rad ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was incubated with amylose resin for 1 h at 4°C and loaded onto a 20 ml chromatography column (Bio-Rad, Hercules, CA, USA). After washing 3 column volumes with wash buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... RNA template (1 μg) was prepared and reverse transcription reaction was performed with iScript Advanced cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA) according to the procedure described in the product’s instruction manual ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A temperature gradient of 25 to 70°C (heating rate 1°C per min) was applied using a MJ minicycler (Bio-Rad, Hercules, CA, USA). All experiments were performed triplicates.
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bioRxiv - Evolutionary Biology 2020Quote: ... touchdowns from 56 °C to 50°C for 1 min and 50 cycles with 95 °C for 30 s and 50 °C for 1 min in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Physiology 2019Quote: ... The remaining supernatant was diluted to 1.5 µg × µl−1^ total protein in lysis buffer and 4X Laemmli sample buffer (Bio-Rad Laboratories AB, Oslo Norway) containing 2-Mercaptoethanol ...
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bioRxiv - Plant Biology 2020Quote: Colonies from TAP agar plates were scraped and suspended in 600 μL liquid TAP media and adjusted to an optical density of 1 at 600 nm using a visible light spectrophotometer (SmartSpec 3000, Bio-Rad, Hercules, CA, USA). The Chlamydomonas suspension was used to inoculate TAP liquid media at a 1:250 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then washed 3X in PBST (10 min) and incubated for 1 h with horseradish peroxidase conjugated anti-rabbit (BioRad 170-6515, lot #: 64033820) or anti-mouse (GE Healthcare UK Limited NA931V ...
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bioRxiv - Neuroscience 2020Quote: ... protein extracts were diluted into a final concentration of SDS 1% and were subjected to vacuum filtration through a 96-well dot blot apparatus (Bio-Rad Laboratories, Hercules, USA) containing a cellulose acetate membrane with pores with a 0.2 μm diameter (Whatman ...
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bioRxiv - Microbiology 2020Quote: ... was performed to detect viral RNA using the CDC standard N2-based SARS-CoV-2 detection assay [35] that was validated for use with the qScript XLT 1-Step RT-qPCR ToughMix (Quantabio, Beverly, MA, USA) on a CFX96 real-time thermocycler (Bio-Rad, Hercules, CA, USA) using a 20 minute RT step and 45 cycle qPCR in a 20 μL reaction volume ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescent dye-conjugated secondary antibodies were used at 1:3000 and the proteins were detected using ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Biochemistry 2022Quote: ... the membrane visualization was facilitated by 1:1 chemiluminescence luminol enhancer solution and peroxide solution using the Bio-Rad imaging system (Molecular Imager® Chemidoc XRS and Bio-Rad imaging system).
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bioRxiv - Immunology 2022Quote: ... Amplification reactions contained a target specific fraction of cDNA and 1 μM forward and reverse primers in iQ™ SYBR® Green Supermix (Bio-Rad, 1708882). Fluorescence was monitored and analyzed in a CFX connect real-time PCR system (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... separated by 8% SDS-PAGE gels at 100V for 1 hour and then transferred to a 0.45 μm pore size nitrocellulose membrane (Bio-Rad, catalog number: 162-0115) at 0.2 A for 1.5 hours in an ice bath ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated for 1 h with either anti-mouse or anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Bio-Rad Laboratories, Hercules, CA). Bands were visualized and subjected to densitometric analysis using an Odyssey Fc system (LI-COR Biotechnology ...
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bioRxiv - Neuroscience 2020Quote: ... protein extracts were diluted into a final concentration of SDS 1% and were subjected to vacuum filtration through a 96-well dot blot apparatus (Bio-Rad Laboratories, Hercules, USA) containing a 0.2 μM cellulose acetate membrane (Whatman ...
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bioRxiv - Plant Biology 2021Quote: ... was performed with gene-specific primers (Supplementary Table 1) using EF1α as internal reference using the CFX Connect Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA) as previously described in (Zandalinas and Mittler ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... After overnight incubation membranes were washed three times with 1x TBST for ten minutes per wash and then incubated with a secondary goat anti-rabbit antibody (BioRad Cat#1706515, 1:10000) for one hour with rocking at room temperature ...
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bioRxiv - Immunology 2022Quote: ... cell debris was removed by centrifugation and clear supernatants were diluted 10-fold and subjected to anion exchange chromatography on gravity fed columns using AG-1 9 8 resin (formate form, 100–200 mesh size, Bio-Rad Laboratories, Hercules, CA). Fractions containing inositol mono ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR was subsequently performed using the iTaq Universal SYBER Green Supermix using the primers listed in Supplemental Table 1 on a CFX96 Real-Time PCR Detection System (Bio-Rad, Richmond, CA, USA). Data were normalized using GAPDH as internal control.