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Citations for Bio-Rad :
5601 - 5650 of 5853 citations for 6 Chloro 5 6 dihydroimidazo 1 5 a pyrido 3 2 e pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... The cDNA was used as template for real-time PCR (primers in Supplemental table 1) using CFV384 Touch™ Real-Time PCR Detection System (Bio-Rad). Housekeeping genes (AT4G34270 ...
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bioRxiv - Biophysics 2021Quote: The removal of DDM in the protein solutions was done by fixing the protein concentration at 1 mg/ml and using 30 mg of BioBeads SM2 (Bio-Rad, CA) per mg of protein ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 1 μg of RNA was subjected to retrotranscription using iScript™cDNA Synthesis Kit (Bio-Rad, Cat. No. 1708890), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... slices were bombarded with biolistic particles created by coating 1.6 μm gold particles (Bio-Rad, #165-2262; ∼1 μg DNA/mg gold) with constructs in mammalian expression plasmids ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated secondary anti-mouse antibody (Amersham; NA931S) and anti-rabbit antibody (Amersham; NA934VS) at 1:10,000 and Clarity western ECL substrate detection kit (Bio-Rad; 170-5060) were used to detect the signals ...
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bioRxiv - Immunology 2019Quote: ... and 1 µg of RNA was used as input for cDNA synthesis using the iScript cDNA synthesis kit (Bio-rad, cat. #: 1708890). cDNA was diluted to 20 ng/µl and 2.5 µl/reaction was used as input for the qPCR reaction ...
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bioRxiv - Developmental Biology 2020Quote: ... 1-20μg of tissue protein to be analyzed were combined with equal parts 2X Laemmli sample buffer (Bio-Rad, catalog 161-0737) containing 10% β-mercaptoethanol ...
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bioRxiv - Biochemistry 2019Quote: ... Gene silencing efficiencies were performed as described previously18 with the following modifications: cDNA was synthesized from 1 μg total RNA using the iScript cDNA Synthesis Kit (Bio-Rad, #1708891) following the manufacturer’s instructions and qRT-PCR was performed with a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Physiology 2020Quote: ... Gene expression was assessed using commercially available Taqman primer/probe sets (Thermo Fischer) or specific oligonucleotide pairs (Table 1) and SYBR® Green supermix (Bio-Rad). Relative expression of the target genes was calculated with the comparative Ct method [43] using glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Developmental Biology 2019Quote: ... Subsequent analysis was conducted on a 12% SDS-PAGE gel and blots were probed with either mouse α-V5 (SV5-Pk1, 1:1000, Bio-Rad), mouse α-HA (HA.11 ...
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bioRxiv - Immunology 2021Quote: ... in 0.1% BSA in 0.1% TBST and subsequently imaged using HRP-conjugated anti-rabbit antibodies (Bio-Rad Cat#172-1019, 1:10000) and Western Lightning Plus-ECL (PerkinElmer Cat#El103001EA ...
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bioRxiv - Microbiology 2021Quote: ... Gels were stained with ethidium bromide (0.5 mg mL-1) and photographed using the Quantity One electrophoresis analysis system (Bio-Rad, CA, USA). Gel images were digitally normalized to a single DNA marker to reduce gel-to-gel banding pattern variability ...
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bioRxiv - Neuroscience 2020Quote: ... Up to 1 μg of RNA was used to synthesise cDNA using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad, 1725035) and PowerUp SYBR Green (Thermofisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Gel electrophoresis was performed on a 1% agarose gel and imaged using Molecular Imager® Gel Doc™ XR (Bio-Rad, USA).
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bioRxiv - Cell Biology 2021Quote: ... and 1 μg of RNA was used to generate cDNA with the iSCRIPT Clear kit (Bio-Rad, Hercules, CA, cat# 170-8891). For the qRT-PCR ...
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bioRxiv - Systems Biology 2021Quote: Samples were normalized to 1 mg/mL protein in 0.1% Azo by the Bradford assay (Bio-Rad, Hercules, CA, USA, Cat# 5000006) using bovine serum albumin to generate a standard curve ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were washed three times in TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Bio-Rad 1,706,515, 1:10,000) secondary antibodies for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribonucleoprotein (RNP) complexes were made at a 9:1 ratio of sgRNA:Cas9 (90pmol:10pmol) in Gene Pulser® Electroporation Buffer (Bio-Rad, 1652677) and incubated for 10 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using iScript Reverse Transcription Supermix for RT-PCR (Bio-Rad Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... All qPCR amplifications were performed in duplicate and each contained 15 µL total volume of 1 × SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, USA), 0.3 µM of each primer ...
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bioRxiv - Microbiology 2022Quote: ... The melt curve analysis was performed at 65°C for 1 minute in CFX connects TM Real-time PCR detection system (Bio-Rad, USA). The primers used are listed inTable 1 ...
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bioRxiv - Neuroscience 2022Quote: ... 1:4 & 1:8) and loaded in triplicates onto a nitrocellulose membrane assembled in a 96-well dot-blot apparatus (Biorad, Cat#1703938). The membrane was then stained with Ponceau (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... TMEM87A in buffer 1 was exchanged into soybean l-α-phosphatidylcholine (Soy PC, MillaporeSigma) with the addition of Biobeads SM2 (Bio-Rad) and an hour incubation at a protein:lipid ratio of 1:10 (corresponding to 0.4 mg purified TMEM87A and 4 mg of Soy PC lipid or 1:50 in buffer (5 mM HEPES pH 7.2 ...
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bioRxiv - Biophysics 2022Quote: ... Electrophoresis was carried out for 1 hour at 90 V using an electrophoresis system (mini–DNA SUB CELL, Bio-Rad, Mississauga, Canada). The DNA fragments were visualized in a Gel Doc XR+ Gel Documentation System (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized using 1 μg of input RNA with an iScript™ cDNA Synthesis Kit and MyIQ thermocycler (both Bio-Rad) and stored at −20 °C overnight ...
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bioRxiv - Plant Biology 2021Quote: The cDNA synthesis was performed using 1 μg of total RNA and iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: cDNA was synthesized using 1 μl of total RNA from melanocytes or iridophores with iScript™ Advanced cDNA Synthesis Kit (#1725038, Bio-Rad) following by 6 cycles of pre-amplification with pooled 47 pairs of MTE primers designed for NanoString CodeSet (SsoAdvanced™ PreAmp Supermix ...
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bioRxiv - Immunology 2020Quote: ... 40 cycles of 15 s 95°C and 1 min 60°C) was carried out using a CFX 96 (BioRad, Hercules, USA) qPCR machine ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA concentration was estimated by nanodrop and 1 μg of RNA was used to synthesize cDNA with iScript cDNA synthesis kit™.(Biorad 1708890) qPCR was performed using Precision™ 2X qPCR master mix (Primerdesign ...
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bioRxiv - Neuroscience 2022Quote: ... macrophages were plated in a 24-well plate containing cover slips and incubated with the Magic Red cathepsin B substrate (1/250) (Bio-Rad, ICT938) for 2h ...
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bioRxiv - Neuroscience 2022Quote: ... then placed in 4% normal goat serum in 0.01M PBS blocking solution for 60 minutes with agitation followed by incubation with rat anti-CD68 (1:500; BioRad, cat no. MCA1957GA) and rabbit anti-MBP (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1 μg of RNA was reverse transcribed into cDNA using BUNiScriptTM cDNA Synthesis Kit for RT-qPCR (Bio-Rad, 170-8891). For pancreas samples ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Expression levels of ICAM-1 and IL6 mRNAs were quantified using SYBR Green-based primers and the iTaq master mix (Bio-Rad Inc) in the Bio-Rad CFX Real-Time PCR detection system (Bio-Rad Inc) ...
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bioRxiv - Immunology 2022Quote: The collected fractions were then pooled by the expected oligomeric state for both human milk and serum and again IgAs were captured on beads with a 1% blocking buffer background (Bio-Rad, Netherlands) and addition of the 7D8-IgA1 and 5D5v2-IgA1 at 0.2 μg/mL ...
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bioRxiv - Neuroscience 2019Quote: ... and cDNA was reverse transcribed from 1 ug of the resultant RNA with the Bio-Rad iScript kit (Bio-Rad 170-8891) in a reaction volume of 20 µl ...
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bioRxiv - Synthetic Biology 2019Quote: ... 3-HB and n-butanol were separated with 5 mM sulfuric acid as the mobile phase and one of two column conditions: (1) an Aminex HPX-87H or Fast Acids anion exchange columns (Bio-Rad Laboratories) at 35 or 55 °C and a flow rate of 0.6 ml min-1 or (2 ...
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bioRxiv - Microbiology 2020Quote: ... containing 2 g l-1 glucose and 1.32 mM K2HPO4 by treating prepared media with a universal chelator (Chelex® 100 Chelating Resin, Bio-Rad 1421253), filtering sterilizing away the Chelex resin ...
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bioRxiv - Microbiology 2021Quote: ... the gel was stained with 1 mg/mL ethidium bromide and visualized with the ChemiDoc XRS system (Bio-Rad Laboratories, CA, USA). Genetic similarities were measured using the Dice coefficient and the clustering was carried out by the unweighted-pair group method with arithmetic averages (UPGMA ...
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bioRxiv - Developmental Biology 2021Quote: ... Following 4x 10 min washes with PBS-T the membrane was incubated for 1 min with Clarity Western ECL Substrate (170-5060, Bio-Rad Laboratories) and imaged with Amersham™ Imager 600 (GE ...
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bioRxiv - Microbiology 2019Quote: ... and visualized with rabbit-α-FLAG (1:3,000) primary and goat-α-rabbit-HRP conjugated secondary antibodies on the ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR was performed using 1:10 dilutions of cDNA synthesis products with the iTaq Universal SYBR green supermix (Bio-Rad; 1725121) on a CFX Connect real-time PCR detection system (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... The separated protein was transferred to a nitrocellulose membrane in Towbin buffer 1× using the Mini Trans-Blot system (Biorad, United States) at 120mA for 120 minutes in ice cold conditions ...
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bioRxiv - Molecular Biology 2019Quote: ... reverse transcription (RT) was carried out with 1 µg of total RNA using the iScriptTM cDNA Synthesis Kit (Bio-Rad, Hercules, CA). Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR ...
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bioRxiv - Physiology 2019Quote: ... 1 cOmplete™ protease inhibitor cocktail EDTA free tablet) and protein concentrations determined using the DC protein assay (Bio-Rad, Hercules, CA). Oxygen Consumption Rate (OCR ...
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bioRxiv - Cell Biology 2021Quote: ... 12.5 ng of cDNA of each sample were pre-amplified using 1 μL of all primers mix (500 nM) in a T100 Thermal cycler (BioRad, Hercules, CA), using the following program ...
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bioRxiv - Plant Biology 2021Quote: The cDNA synthesis was performed using 1 μg of total RNA and iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and analyzed for the expression of the exosome production-related genes listed in Appendix Table 1 by RT-PCR on a CFX Connect Real-Time System (Bio-Rad Laboratories).
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bioRxiv - Neuroscience 2021Quote: ... was incubated at 65°C for 10 min and at 85°C for 1 min into a T100™ thermocycler (Bio-Rad). The heated lysate was subsequently used in cDNA synthesis with SuperScript™ III Reverse Transcriptase kit in a total volume of 20 μl following manufacturer protocol (Invitrogen™) ...
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bioRxiv - Physiology 2021Quote: ... were used for real-time PCR with gene-specific primers (Supplemental Table 1) and Tbp as a house keeping gene on a CFX96™ Real-Time PCR Detection System (Bio-Rad).
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bioRxiv - Genomics 2021Quote: ... Reverse transcription (RT) was performed on 1 μg of DNase-treated total RNA using the iScript cDNA synthesis kit (Bio-Rad #1708890), with the same amount of total RNA diluted in water as negative controls for RT-PCR ...