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Citations for Bio-Rad :
5351 - 5400 of 5853 citations for 6 Chloro 5 6 dihydroimidazo 1 5 a pyrido 3 2 e pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The samples were then subjected to SDS-PAGE at a constant voltage of 100 V for 1 hour at room temperature and transferred to nitrocellulose membrane (BIO-RAD) at a constant voltage of 30 V at 4°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... The product yield for each condition was estimated by resolving 10% of the reaction on a 1% EtBr-agarose gel and quantifying the band intensity of the resulting amplicon with ImageLab software (Bio-Rad). The 1.6-kb amplicon was gel-extracted using the QiaQuick Gel Extraction Kit (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... Signals were developed by incubating with 1/5000 fold dilution of anti-rabbit or anti-mouse secondary antibodies for 1 h at room temperature and quantified using the Image Lab Software on a ChemiDoc XRS system (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed with 1 μg total RNA using iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad 170-8841). cDNA equivalent to 20 ng of total RNA was then amplified in duplicate using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad 172-5274 ...
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bioRxiv - Cell Biology 2020Quote: ... They were immediately transformed by bombardment with plasmid-coated 1-μm gold particles with a helium-driven particle accelerator (PDS-1000/He; Bio-Rad) using 1350 psi rupture disks and a vacuum of 28 inches of mercury ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight at 4 °C with the following primary antibodies (1:100, in blocking buffer): mouse anti-human MBP (BMK-13, antihuman MBP (BMK-13, MCA5751 Bio-RAD), rabbit anti-human PD-L1 (E1L3N ...
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bioRxiv - Developmental Biology 2021Quote: ... and OPN) and control (HPRT) transcripts were amplified (Supplemental Table 1) using SsoAdvanced Universal SYBR Green Supermix (Biorad, Hercules, CA, USA). Established TaqMan assays were used to determine the expression of Lgr6 and TBP intra-well controls ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were separated by electrophoresis at 70V for 15 minutes followed by 100V for approximately 1 hour using 10x Tris/Glycine/SDS running buffer (BioRad, 1610772).
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bioRxiv - Neuroscience 2021Quote: ... analysis was performed through reverse transcription on 0.5-1 μg of total RNA using the iScript cDNA synthesis kit according to the manufacturer’s instructions (Bio-Rad, Hercules, CA). The cDNA was diluted 1:20 in nuclease-free water ...
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bioRxiv - Microbiology 2019Quote: ... The copy number of a host gene RPP30 was determined using 1 μL of HEX-labeled PrimePCR RPP30 (Bio-Rad, 10031243) in the reaction to calibrate the amount of DNA input ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were heated to 50°C for 1-10 min before addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594), vortexing vigorously for 5 s before pipetting in plug mould (Bio-Rad 1703713 ...
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bioRxiv - Molecular Biology 2020Quote: Following electrophoretic separation, oligonucleotide-protein complexes were transferred by electro-blotting (330 mA, 1 h) onto specific positively charged nylon membranes (Bio-Rad) and detected using an anti-digoxigenin antibody conjugated with alkaline phosphatase.
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bioRxiv - Genomics 2020Quote: ... The indexed amplicons were mixed based in relative fluorescence units and run on a 1% agarose gel with Sybr Safe and gel extracted using a freeze and squeeze column (Bio-Rad). The product was quantified using Kapa Illumina Quant kit.
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... supplemented with 1 mM DTT for reduction of disulfide bonds and protein concentration was determined by Quick-start Bradford assay (Bio-Rad). Proteins were alkylated with 5.5 mM chloracetamide (45 min ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA was produced from 1 μg of purified RNA using the iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad Laboratories). qPCR reactions were performed with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2019Quote: ... secondary antibodies were then incubated for 1 hour at room temperature followed by detection of bands by chemiluminescence in ChemiDoc XRS+ (Bio-Rad).
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bioRxiv - Neuroscience 2019Quote: ... 0.02% NaN3 and 1 mM DTT at pH 7.4 at 37°C in a thermal cycler (BioRad DNA Engine PTC-200). In some cases ...
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bioRxiv - Immunology 2020Quote: ... A 1 µg sample of total RNA was reverse transcribed to complementary DNA using the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Alternatively broken cyst walls either before or after digestion with trypsin were reconstituted in 1× reducing SDS/PAGE loading buffer and run on a 4–20% precast polyacrylamide TGX gel (Bio-Rad). Bands stained by colloidal Coomassie blue were excised and washed with 50 mM NH4HCO3/acetonitrile (ACN) ...
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bioRxiv - Genomics 2019Quote: Differentiating and IFC-305 treated HepaRG cells cultured for 1 week were trypsinized and counted using TC20 automated cell counter (Bio-Rad) with dual chamber slides and trypan blue (Bio-Rad 1450003) ...
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bioRxiv - Neuroscience 2019Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4°C and subsequently washed in TBST and incubated for 1 hr at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
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bioRxiv - Physiology 2019Quote: ... Antennal cDNA was synthesized from 1 µg of antennal total RNA from each species using iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions (Clontech ...
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bioRxiv - Cancer Biology 2020Quote: ... Aqueous droplets with a 20-µl volume containing final concentrations of 1× Droplet Digital PCR Supermix for probes (Bio-Rad Laboratories) and 100 ng of the genomic DNA template were generated ...
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bioRxiv - Microbiology 2019Quote: ... 39) and 1 µg of total RNA was reverse transcribed to cDNA using the iScript Select cDNA Synthesis Kit (Bio-Rad) with random hexamers ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The membranes were then incubated with HRP-conjugated secondary antibodies (1:5000, anti-Gt, #61-1620, Invitrogen; anti-Rb, #170-5046, Bio-Rad; or mouse IgG kappa binding protein ...
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bioRxiv - Molecular Biology 2019Quote: ... qPCRs were performed using 2ul of 1:100 diluted first PCRs as a template with qPCR primers (Table S4) and iTaq Universal SYBR Green Supermix (Bio-Rad) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2019Quote: ... A one in 10 dilution of cDNA was used for qPCR reaction mixture with final concentration of 1× SsoAdvanced universal SYBR super mix (Bio-Rad) and 1×PrimePCR assays for the designated target ...
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bioRxiv - Neuroscience 2021Quote: ... of each sample was loaded on an 8-16% (for TSPO, 3b-HSD, and StAR) or 4-20% (for mERα, caveolin-1, and PKA) SDS precast gels (Bio-Rad) and separated by electrophoresis ...
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bioRxiv - Biochemistry 2021Quote: ... The membranes were then incubated with secondary antibodies (1:10000, goat anti-rabbit or goat anti-mouse IgG HRP conjugate (Bio-Rad) for 1 hour and detection was performed using the Immobilon-luminol reagent assay (EMP Millipore).
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bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary antibodies (Supplementary Table 1) overnight at 4°C and visualised with HRP-conjugated anti-IgG secondary antibodies and enhanced chemiluminescence substrates (Bio-Rad). The expression of PDHX or calnexin was used as a loading control.
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bioRxiv - Neuroscience 2021Quote: ... for 10 minutes followed by 15 minutes of tris-buffered saline with 0.25% Triton X-100 then blocked for 1 hour in tris-buffered saline with Tween 20 solution at 0.05% (BioRad #161-0781) supplemented with sterile 2% Normal Donkey serum (Abcam #ab138579 ...
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bioRxiv - Biochemistry 2021Quote: ... The melting curves were measured at 1°C/min at the FRET channel in triplicate measurements and the data analyzed with CFX Manager™ (BioRad).
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bioRxiv - Biochemistry 2021Quote: ... Western blot was performed using 8 or 10% mini gels followed by wet transfer (1 h, 100 V) to Western grade nitrocellulose (Bio-Rad). Blots were incubated in Intercept blocking buffer (LI-COR ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were replenished with compounds every 24 h and counted on days 1-9 using automated cell counter (Bio-Rad). KYSE-520 cells were plated onto 96-well plates (500 cells per well ...
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bioRxiv - Neuroscience 2021Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4°C and subsequently washed in TBST and incubated for 1 hr at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
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bioRxiv - Neuroscience 2021Quote: ... USA) that were diluted 1/5000 in TBS-T and revealed by enhanced chemiluminescence plus (ECL) solution (Biorad Laboratories, ON, Canada). β-actin was used to ensure equal protein loading ...
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bioRxiv - Cancer Biology 2021Quote: ... were run on a 1 mm thick 10% SDS-PAGE gel and stained with CBB G-250 staining solution (Bio-Rad) at RT for 1.5 h ...
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bioRxiv - Cancer Biology 2020Quote: ... Revelation was performed using secondary antibodies conjugated to horse-radish peroxidase (GE-Healthcare) diluted 1:2,000 in saturation solution and Opti-4CN™ colorimetric kit (Bio-Rad). ImageJ software was used to measure the relative intensity of protein bands.
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription of 1 μg of RNA in a total volume of 20 μl was performed with iScript cDNA Synthesis Kit (Bio-Rad) prior to real time PCR (iCycler ...
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bioRxiv - Physiology 2020Quote: ... We synthesized a total of 500 ng (pituitary) and 1 μg (gonad) of RNA into cDNA using the iScript cDNA synthesis kit (Bio-Rad) in 20 μl reactions according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Electrophoresis of PCR products was done on EtBr-stained 1% agarose gels and photographed on a Gel Doc XR instrument (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... The melting curves were measured at 1°C/min at the FRET channel and the data analyzed with CFX Manager™ (BioRad).
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibody ALP conjugate (1:40,000 dilution) was also used and its blots were analysed on Gel doc system (BioRad, CA, USA).
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bioRxiv - Plant Biology 2021Quote: ... benthamiana leaves was infiltrated with 1 mM luciferin 40 h after inoculation of the Agrobacteria and then imaged under the Gel Doc XR+ System (Bio-Rad) with the Bolt mode ...
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bioRxiv - Synthetic Biology 2021Quote: ... We performed cDNA synthesis with 0.5-1 μg of total RNA using the iScript Select cDNA Synthesis Kit (Bio-Rad Laboratories). We performed Real Time quantitative PCR (RT-qPCR ...
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bioRxiv - Microbiology 2020Quote: ... RNA purity and integrity was verified by 1% (w/v) agarose gel electrophoresis in an Experion Automated Electrophoresis System (Bio-Rad). One µg of RNA was reverse-transcribed into cDNA (iScript Kit ...
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bioRxiv - Microbiology 2020Quote: ... or anti-PfADF1 (1:2000, (Wong et al, 2011)) and HRP-coupled goat anti-mouse or -rabbit secondary antibody (1:5000, STAR120P/STAR121P, Bio-Rad). sBlots were washed in PBST and detected using ECL reagent (Amersham ...
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bioRxiv - Systems Biology 2020Quote: ... and used 500 ng - 1 ug RNA to make cDNA using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA). 0.1 of cDNA (i.e ...
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bioRxiv - Plant Biology 2021Quote: ... SybrGreen real-time quantitative PCR experiments were performed with a 1:20 dilution of cDNA using a CFC384 Real-Time System (Bio-Rad). Data were analyzed with the comparative 2ΔΔCt method using the geometric mean of Ef1α and PP2A as housekeeping genes ...
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bioRxiv - Plant Biology 2021Quote: ... ∼1 ug of RNA from resulting extraction was used as template in standard cDNA synthesis reaction (BioRad iScript cDNA Synthesis Kit). 200 ng of resulting cDNA was used in qRT-PCR reactions to quantify POL expression levels (PowerUp SYBR Green 5x Master Mix-Thermo) ...