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Citations for Bio-Rad :
5651 - 5700 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Bound proteins were eluted by boiling the beads in 2x Laemmli Sample Buffer (BIO-RAD). Samples were then loaded onto a NuPAGE 4-12% Bis-Tris Protein Gel (Thermo ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting protein lysates were resuspended in an equal volume 2X Laemmli (BioRad,161-0737) + dithiothreitol (Fisher BioReagents ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein samples (10 μg) were separated on 4 % - 15 % Bis-tris gels (Bio-Rad Laboratories) and wet-transferred onto a PVDF membrane ...
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bioRxiv - Developmental Biology 2022Quote: ... and loaded on 4–15% Mini-PROTEAN®TGX™ precast protein gels (Biorad, 4561083). Proteins were separated by electrophoresis at 70V for 15 minutes followed by 100V for approximately 1 hour using 10x Tris/Glycine/SDS running buffer (BioRad ...
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bioRxiv - Systems Biology 2022Quote: ... Cell lysates were analyzed using the Bio-Plex Protein Array system (Bio-Rad, Hercules, CA) according to the suppliers protocol as described previously [19] ...
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bioRxiv - Biochemistry 2022Quote: ... Excess dye was separated from the protein using a Biogel-P4 column (Bio-rad laboratories). The concentration of Rtt105 calculated spectroscopically using extinction coefficient ε280= 18,450 M-1cm-1 and labeling efficiency was calculated using the Cy5 absorbance signal and ε650= 250,000 M-1cm-1 ...
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bioRxiv - Neuroscience 2021Quote: ... Protein samples were loaded on pre-cast 4-20% TGX polyacrylamide gels (Criterion, Bio-Rad). Electrophoresis was performed in 1x pre-mixed electrophoresis buffer (10 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: Milk proteins were separated by SDS PAGE on Criterion TGX 4-20% gels (Bio-Rad). Following wet transfer onto a Hybond ECL membrane (Cytiva ...
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bioRxiv - Molecular Biology 2020Quote: The total protein concentration of the soluble fractions was measured by Lowry assay DC (BioRad). For the melt-curve CETSA MS experiments only samples from highest compound concentration (20μM ...
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Integrated requirement of non-specific and sequence-specific DNA binding in MYC-driven transcriptionbioRxiv - Molecular Biology 2020Quote: ... Quantification of protein levels was performed using the Image Lab software (Bio-Rad, version 4.0).
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bioRxiv - Molecular Biology 2020Quote: Proteins were resolved on an SDS-PAGE gel and transferred to a nitrocellulose membrane (BioRad). In L1-ORF2p western blots ...
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bioRxiv - Neuroscience 2021Quote: ... 50 μg of protein was loaded into each lane of Mini-Protean TGX gels (Biorad) and ran at 120 V for 1.5 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Total proteins were visualized via staining with Coomassie Brilliant blue R250 staining solution (Bio-Rad). Densitometric quantification was performed with ImageJ.
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bioRxiv - Molecular Biology 2021Quote: ... purified proteins were loaded on 4–20% Mini-PROTEAN TGX Gel (Bio-rad 456-1093) and Western blotting was performed with anti-GFP (Abcam 290 ...
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bioRxiv - Molecular Biology 2021Quote: Purified SARS-CoV-2 N protein was electrophoresed on 4-10% SDS-PAGE (Bio-Rad) and stained with Coomassie Brilliant Blue G-250 (CBBG-250) ...
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bioRxiv - Cell Biology 2022Quote: ... Quantifications of proteins from intestinal tissues were performed on a ChemiDoc Imaging System (Bio-rad). SUMO2/3-conjugated proteins levels (above 50 kDa ...
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bioRxiv - Microbiology 2021Quote: ... run on a native gel (12% Mini-PROTEAN® TGX™ Precast Protein Gels, BioRad), and transferred to a membrane using BioRad’s Trans-Blot Turbo Transfer System (high molecular weight settings) ...
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bioRxiv - Biophysics 2019Quote: ... Protein ubiquitylation was analyzed by SDS-PAGE using 4-15% Mini-PROTEAN-TGX gels (BioRad) and immunoblotting using anti-MBP antibodies.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Protein bands were quantified using a ChemiDoc™ MP Imaging system (Bio-Rad Co., USA), and analyzed using the Image Lab software (Bio-Rad Co. ...
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bioRxiv - Pathology 2019Quote: ... proteins separated on the gel were transferred to PVDF (polyvinylidene difluoride, 0.2 μm, Bio-Rad) membranes in transfer buffer (0.1 M Tris ...
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Differential turnover of Nup188 controls its levels at centrosomes and role in centriole duplicationbioRxiv - Cell Biology 2019Quote: ... 15-50 μg of protein was separated on a 4-20% gradient gel (Bio-Rad) and transferred onto 0.2 µm nitrocellulose (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... When required proteins were resolved in 4-20% Mini-PROTEAN® TGX Precast Gel (BioRad) and transferred to nitrocellulose membrane overnight in 48mM Tris ...
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bioRxiv - Pathology 2019Quote: ... Extracts with equal amounts of proteins were solubilized by SDS sample buffer (BioRad, Califonia, USA), separated by SDS-PAGE ...
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bioRxiv - Microbiology 2019Quote: ... Separated proteins were transferred to polyvinylidene difluoride (PVDF) membranes (Immun-Blot, Bio-Rad, California, USA) in blotting buffer (40 mM Tris ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were transferred to a nitrocellulose membrane using the Trans-blot turbo system (Bio-Rad), mixed molecular weight protocol (2.5A ...
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bioRxiv - Immunology 2019Quote: ... Proteins were transferred to 0.45 um PVDF membranes (Immobilon) in Tris/Glycine buffer (Bio-rad) supplemented with 20% methanol ...
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bioRxiv - Developmental Biology 2019Quote: ... Proteins were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Bio-rad Laboratories).
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bioRxiv - Microbiology 2019Quote: ... Cell proteins were resolved by SDS-PAGE using Mini-PROTEAN TGX Precast Gels (Bio-Rad). Immunoblotting was carried out using the following primary antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... Resolved proteins were transferred to nitrocellulose membranes using the Trans-Blot® Turbo system (BioRad) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... 60 μg of protein lysate was separated by SDS-PAGE (BioRad Miniprotean TGX 4-20%) and transferred onto nitrocellulose membranes ...
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bioRxiv - Immunology 2019Quote: ... proteins were visualized by SuperSignal chemiluminescent reaction (Pierce Biotechnology) in a ChemiDoc station (Bio-Rad).
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bioRxiv - Cell Biology 2019Quote: ... Protein lysates were ran on Mini-Protean TGX 4-20% gradient SDS-PAGE gels (BioRad) and transferred to 0.2 µm PVDF Trans-blot Turbo membranes (BioRad ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein was separated on TGX-stain free pre-cast 4–20% SDS gels (Biorad 4568094) at 200V for 30’ ...
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bioRxiv - Immunology 2020Quote: ... The concentration of total protein obtained was determined using the Bradford assay (Bio-Rad, USA). Thirty micrograms (30µg ...
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bioRxiv - Immunology 2020Quote: ... The concentration of total protein obtained was determined using the Bradford assay (Bio-Rad, USA). Thirty micrograms (30µg ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein supernatant was collected and mixed with 4x Laemmli sample buffer (Bio-Rad, 161-0747). Total 20–40 µg protein was loaded and separated by SDS-PAGE gel electrophoresis ...
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bioRxiv - Genetics 2020Quote: ... 160 μg of protein was separated on 4-15% Mini-Protean TGX gels (BioRad, Inc.), and then transferred to nitrocellulose ...
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bioRxiv - Cancer Biology 2021Quote: ... the proteins were transferred onto nitrocellulose membranes using a commercial transfer kit (Bio-Rad #1704158). After transfer ...
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bioRxiv - Cell Biology 2020Quote: ... Protein lysates were then resolved in SDS-PAGE and transferred onto PVDF membrane (Bio-Rad). The membrane was blocked with 5% non-fat milk in PBST for 1 hr ...
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bioRxiv - Neuroscience 2020Quote: ... supernatant was removed to a new tube and protein concentration assayed by Bradford (Bio-Rad). From each tissue sample ...
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bioRxiv - Neuroscience 2020Quote: ... Loading was 200 µg of protein as determined using a modified Bradford reaction (BioRad Laboratories). Quantification of Shh was performed with Rat Shh ELISA Kit (FineTest ...
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bioRxiv - Neuroscience 2020Quote: ... Loading was 20 µg of proteins as determined using a modified Bradford reaction (BioRad Laboratories). Proteins were separated in 7–15% SDS-PAGE and electrophoretically transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2021Quote: ... Resolved proteins were transferred to PVDF membranes using the Trans-Blot Turbo Transfer System (BioRad) and probed with either a 1:10,000 dilution of α-FLAG HRP-conjugated antibody (Merck ...
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bioRxiv - Neuroscience 2020Quote: ... Forty-eight μg of protein were loaded onto a Mini-Protean TGX gel (Bio-Rad) and electrophoresis was run on an ice-bed ...
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bioRxiv - Cell Biology 2021Quote: ... Protein samples were analyzed by Western blotting and detected using the ChemiDoc MP system (BioRad) as per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... together with the Precision Plus Protein Dual Color Standards molecular weight marker (Bio-Rad; 1610374). The separated proteins were transferred to a PVDF membrane (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein blot bands were visualized using Clarity Western ECL Substrate (Bio-Rad, Hercules, CA; 1705061) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific ...
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bioRxiv - Bioengineering 2019Quote: ... Then 5-10ug protein per sample was loaded onto 4%-20% gradient gel (Bio-rad) and the gel was run for 2 hours at 90mV ...
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bioRxiv - Biochemistry 2021Quote: ... Gel electrophoresis separated proteins were transferred onto a PVDF membrane using a Turboblotter (Bio-Rad). The membrane was first blocked with B-TBST (TBS buffer with 0.05% Tween-20 and supplemented with 5% blotting grade non-fat dry milk ...
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bioRxiv - Biochemistry 2021Quote: ... protein bands were visualised colorimetrically using the AP conjugate substrate kit (Bio-Rad, Munich, Germany) according to manufacturer’s instructions.